• 한국정보전자통신기술학회논문지
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• 제10권2호
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• pp.176-183
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• 2017

대용량 EEPROM 메모리를 테스트하는 경우 erase time과 program time이 많이 걸리는 문제가 있다. 또한 신뢰성 테스트를 진행하면서 각 스텝마다 EEPROM 셀의 문턱전압 VT를 테스트할 필요가 있다. 본 논문에서는 512kb EEPROM 셀 검증용 모듈 회로를 설계하였으며, negative VTE를 갖는 split gate EEPROM의 VT 측정을 위한 CG(Control Gate) 구동회로를 제안하였다. 제안된 CG 구동회로는 erase VT를 측정하기 위해 -3V~0V의 negative 전압이 인가될 수 있도록 asymmetric isolated HV (High-Voltage) NMOS 소자를 사용하였다. 그리고 test time reduction 모드에서는 even page, odd page, chip 단위로 erase나 program 수행이 가능하도록 회로를 설계하므로 512Kb EEPROM 전체 메모리를 erase하거나 program할 때 시간을 even page와 odd page를 이용하는 경우는 4ms, chip 전체로 하는 경우는 2ms로 테스트 시간을 줄일 수 있었다.

• 대한영양사협회학술지
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• 제14권1호
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• pp.1-12
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• 2008

The Purposes of this study were to assess high school students' nutrient consumption at the ordinary time and to analyze nutrient contents and nutrition consumption of lunch. The questionnaires for self-reported food consumption were distributed to 210 students enrolled in a boy's high school and a girl's high school located in Seoul area. A final response rate was 87.1%, excluding responses that had significant missing data. Data of self-reported food consumption and BMI(Body Mass Index) were analyzed with descriptive analysis and t-test using SPSS Win(ver. 12.0). To measure the serving size and the waste amount, data were collected for three meals in a three day period at each school. A weighed plate method was employed to measure plate wastes and consumption of the menus served. Nutrient analyses for the served and consumed menus were performed using CAN-PRO. The result of nutrient intake determined by self-reported food consumption demonstrated 74.2% of boys and 70.0% of the girls did not meet EAR(Estimated Average Requirement) for calcium. In addition, the data collection showed that 60.2% boys and 26.7% girls did not consume EAR for Vitamin B$_1$. Nutrient analyses of the served and consumed menus at school lunch were compared with 1/3 of the Dietary Reference Intakes(DRIs) for this age group. The served menus did not meet 1/3 of the Recommended Intake(RI) for calcium, iron, and vitamin B$_2$. In contrast, the menu provided to students exceeded almost five times(490%) more than the sodium needed for 1/3 of the Adequate Intake(AI). Considering the amount of the students' plate waste, intake of vitamin A and vitamin C were below 1/3 of the RI, and calcium, iron, and vitamin B$_2$ intake were also reduced. Students' sodium consumption still exceeded 1/3 of the AI at 340.0%.

• Journal of Biosystems Engineering
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• 제34권1호
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• pp.50-56
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• 2009

Surface plasmon resonance (SPR) biosensor has been used to detect many biochemical reactions, because this label-free sensor has high sensitivity and rapid response. The reactions are monitored by refractive index changes of the SPR biosensor. Iprovalicarb is protective, curative, and eradicative systemic fungicide introduced by Bayer AG in 1999. It has potential far control of downy mildew infesting onion, cucumber, grape and melon, late blight infesting tomato and potato, and anthracnose infesting watermelon and pepper. It is strictly limited to the maximum residue limit. In this study, the applicability of a portable SPR biosensor (Spreeta, Texas instrument, TX, USA) to detect the iprovalicarb residue was examined. The sensor chip was adopted to detect the reaction of iprovalicarb to immobilized iprovalicarb-antibody. The binding of the iprovalicarb onto the biosensor surface was measured by change of the refractive index (RI). Characteristics of the sensor chip including specificity, sensitivity, stability, and reusability were analyzed. In calibration test for seven levels of iprovalicarb concentration (0.32 to 5,000 mg/L) with three replications, a Sigmoidal model with Hill function was obtained between relative RI value and the iprovalicarb concentration with R-square of 0.998. It took 30 minutes to complete a set of detecting assay with the SPR biosensor.

• The Plant Pathology Journal
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• 제15권3호
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• pp.137-143
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• 1999

A novel chromosomal gene tagging technique using a specific fragment of the fatty acid desaturase-like open reading frame (des-like ORF) from the tox-argK gene cluster of Pseudomonas syringae pv. phaseolicola was developed to identify Xanthomonas spp.released into the environment as biocontrol agents. X. campestris pv. convolvuli FB-635, a pathogen of Convolvulus arvensis L., (bindweed), was chosen as the organism in which to develop and test the system. A 0.52 kb DES fragment amplified from P. syringae pv. phaseolicola C-199 was inserted into pGX15, a cosmid clone containing a 10.3 kb Eco RI-HindIII fragment derived from the xanthomonadin biosynthetic gene cluster contained in plasmid pIG102, to create a pigG::DES insertion. The 10.8 kb EcoRI-BamHI fragment carrying the pigG:: DES insertion was cloned into pLAFR3 to generate pLXP22. pLXP22 was then conjugated into X. campestris pv. convolvuli FB-635 and the pigG::DES insertion integrated into the bacterial chromosome by marker exchange. Rifampicin resistant, tetracycline sensitive, starch hydrolyzing, white colonies were used to differentiate the marked strain from yellow pigmented wild-type ones. PCR primers specific for the unique DES fragment were used for direct detection of the marked strain. Result showed the marked strain could be detected at very low levels even in the presence of high levels of other closely related or competitive bacteria. This PCR-based DES-tagging system provides a rapid and specific tool for directly monitoring the dispersal and persistence of Xanthomonas spp.released into the environment.

• Computers and Concrete
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• 제22권5호
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• pp.481-492
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• 2018

In this paper, a new hybrid fiber system (NHFS) is investigated for the application of slab. The steel fiber, polyvinyl alcohol (PVA) fiber and calcium carbonate ($CaCO_3$) whisker is added to form NHFS. The four-point bending test is carried out on the flexural properties of slab with plain, steel fiber, traditional hybrid fiber system (THFS) and NHFS reinforced cementitious composites. The flexural behavior is evaluated by ASTM C1018-97, JCI-SF4 and post-crack strength (PCS) technique. The evaluation parameters of flexural toughness such as toughness index (TI), equivalent flexural strength (EFS) and PCS are determined. The size of slab specimens is $15mm(thickness){\times}50mm(width){\times}200mm(length)$. The results show that adding $CaCO_3$ whisker to THFS can significantly improve the flexural strength, TI, EFS, PCS of the slab. The empirical relation between reinforcing index ($RI_v$) and flexural parameters show that flexural parameters of slabs increase first and then decrease; which indicates that optimum $RI_v$ values can be helpful in the considering the mix design of steel-PVA fibers-$CaCO_3$ whisker composites for achieving the desired flexural-related properties. The scanning electron microscopy is performed to observe the micro-morphological characteristics of the fracture surface, which proved the positive hybrid effect among the different fibers in cementitious composites. The NHFS can arrest the generation and propagation of the crack from micro to macro level.

• 한국지반공학회논문집
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• 제19권3호
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• pp.93-104
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• 2003

간단한 제하/재재하 구성방정식을 이용하여 기존의 교란상태개념(DSC)모델을 수정하였고, 그 수정된 DSC모델을 비선형 동역학적 유한요소 프로그램인 DSC_DYN2D에 수치해석적으로 접목(implementation)하였다. 본 연구에서 이용한 구성방정식 DSC 모델은 상대적으로 손상받지 않은 상태(RI)를 정의하기 위하여 HiSS모델을 이용하였고, 완전파괴상태(FA)는 한계상태모델을 이용하였다. 이렇게 수정된 DSC_DYN2D 프로그램을 이용하여 동하중을 받는 현장파일 및 그 주변 지반의 거동을 수치해석적으로 모사 하였다. 해석 결과를 기존의 HiSS모델을 이용한 해석결과와 비교, 분석하였다. 비교결과, DSC모델을 이용하여 해석한 결과가 HiSS모델로 해석한 결과보다 현장계측과 유사하였다. 이를 통해 DSC모델을 이용한 수치해석이 복잡한 점토-파일 접촉면 상호작용 거동을 실제적으로 잘 모사할 수 있다고 판단된다.

• 한국지반공학회논문집
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• 제36권7호
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• pp.29-40
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• 2020

본 연구는 제주도 현무암의 함수상태가 역학적 특성에 미치는 영향을 평가하기 위하여 다양한 실내시험을 수행하였다. 시험편은 제주 북서부의 상가리와 어음리에서 채취한 현무암으로 각각 20개씩 제작하였다. 시험은 포화 및 건조상태로 구별하여 진행하였으며, 이를 바탕으로 함수상태에 따른 물성 및 역학적 특성간의 관계를 고찰하였다. 기존 연구결과들과 함께 분석한 결과, 포화상태의 경우 건조상태보다 일축압축강도, 압열인장강도 및 탄성계수가 비슷한 비율로 감소함을 알 수 있었다. 또한 압열인장강도와 일축압축강도는 선형비례관계에 있으며, 함수상태는 이 관계에 큰 영향을 주지 않았다.

• The Korean Journal of Ecology
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• 제25권4호
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• pp.241-245
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• 2002

During the breeding season of 1998, breeding ecology of the Great Reed-Warbler (Acrocephalus arundinaceus orientalis) was studied at Yangsoo-ri and Yongdam-ri of the Yangpyung-gun, Kyunggi province, Korea. Egg-weight (CV: 6.25) was more variable than either length or breadth, and breadth was the least variable of the measures. Significant variations in overall egg-weight occurred between clutches, and that more of the total variation in egg-weight and shape are due to inter-clutch variation as to intra-clutch variation when the data were pooled. The last egg tends to be larger than the remaining eggs in the clutch of the Great Reed-Warbler, suggesting the Great Reed-Warbler may adopt the brood-survival strategy. When method 3 was used, the most common incubation period is 12 days. In the Great Reed-Warbler, the length of the incubation period was related to clutch-size when method 1 (r=0.485, p<0.05) and method 2 (r=0.621, p<0.01) were employed, but not related to egg weight. The averagee number of days of hatching asynchrony was 2.5, raging 0.5~2.5. Asynchronous hatching was related to the clutch size (r=0.66, p<0.01). Hatching sequence was closely related to the laying sequence (r=0.93, p<0.001), suggesting Great Reed-Warblers incubate their eggs before clutch completion. The effect of egg weight on hatching asynchrony was found in Great Reed-Warblers (t-test, p<0.01).

• 미생물학회지
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• 제24권1호
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• pp.7-11
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• 1986

Pseudomonas의 분해계 플라스미드와 이들에 유용한 벡터를 개발하기 위해서 본 연구실에서 분리, 보존하고 있는 Pseudomonas 중에서 P.putita KU 190으로부터 하나의 플라스미드를 분리하여 그 특성을 해석코저 하였다. size marker로 RP4와 pSy343를 사용하여 플라스미드의 크기를 결정한 바 41Kb로 나타났으며, 이 플라스미드를 pKU 41라 명명하였다. 제한효소 Bglll, BamHl, Eco Rl, HindIII, Sall등으로 이 플라스미드를 소화하여 이들의 제한효소 pattern을 조사한 결과, Bg III가 1. BamHl 3, Hin dIll는 3, EcoRI은 6 그리고 SalI은 13개 이상의 절단부위를 가지고 있었다. 이 제한효소의 절단부위, 토막들의 크기로부터 BamHI, HindIII에 대한 지도를 작성하였다. 플라스미드의 생울학적 기능을 조사하기 위하여 탄화수소 자화능에 대한 큐어링 실험을 하였으나 이로부터 뚜렷한 관련성 여부를 관찰할 수 없었다.

• 미생물학회지
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• 제25권4호
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• pp.282-289
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• 1987

Pseudomonas putida KU816에서 분리한 플라스미드 pKU10의 여러가지 특성을 조사하고 그 제한 효소 지도를 작성하였다. pKU 10은 암펴설런, 테트라사이클린, 클로람페니콜에 대한 내성 유전자를 갖는 작은 R factor로서 마이토마이신 C에 의하여 큐어링 된다. 플라스미드의 크기는 9.4Kb로 측정되었다. pKU 10은 Pseudomonas와 E.coli블 숙주로 하였을 때 안정하게 형질 발현이 되다. 또한 pKU 10의 불화합성균은 IncP-I으로 조사되었다. Eco RI, Xho I. SaiI, BglII, SmaI은 pKU 10 DNA를 한 부위에서 자르고, Pst I은 두 부위, Hind Ill는 여섯 부위에서 자른다. 제한 효소 지도는 제한 효소를 이중, 삼중으로 완전 소화시키거나, 부분 소화시켜서 얻었다. pKU 10은 Pseudomonas속에서 유용한 클로닝 벡터호 이용된 것으로 기대된다.