• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.20 no.2
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• pp.87-96
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• 2008

Flow condensation heat transfer coefficients(HTCs) of R123 and R245fa are measured in a horizontal plain tube. The main test section in the experimental flow loop is made of a plain copper tube of 9.52 mm outside diameter and 530 mm length. The refrigerant is cooled by passing cold water through an annulus surrounding the test section. Tests are performed at a fixed saturation temperature of $50\;{\pm}\;0.2\;^{\circ}C$ with mass fluxes of 50, 100, $150\;kg/m^2s$ and heat flux of $7.3{\sim}7.7\;kW/m^2$. Heat transfer data are obtained in the vapor quality range of $10{\sim}90%$. Test results show that the flow condensation HTCs of R245fa are overall 7.9% higher than those of R123 at all mass fluxes. The pressure drop of R245fa is smaller than that of R123 at the same heat flux. In conclusion, R245fa is a good candidate to replace ozone depleting R123 currently used in chillers from the view point heat transfer and environmental properties.

• Journal of Life Science
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• v.11 no.2
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• pp.120-125
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• 2001

The pyrR gene of the pyrimidine biosynthesis (pyr) operon of the thermophile Bacillus caldolyticus, encoding a uracil phosphoribosyltransferase (UPRTase), turned to rely as a pyr operon regulator. It has been proposed that PyrR mediates transcriptional termination-antitermination at three intercistronic regions of the par operon (S.-Y Ghim and J. Neuhard, J. Bacteriol.,176, 3698-3707, 1994). In this research, a plasmid carrying the pyrR region of B. caldolyticus could restore a pyrimidine regulation in a pyrR mutant of B. subtilis. Expression of pyrR was found to increase 6-7 fold during pyrimidine starvation. Additionally, a highly conserved nucleotide sequence which may constitute the binding site for a PyrR protein (PyrR-binding loop) in transcript was staggested. Alternative antiterminator and terminator structures involving three conserved motifs in front of the pyrR, pyrP and pyrB genes, respectively, are proposed to account for the observed regulation pattern.

• Journal of Power System Engineering
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• v.18 no.4
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• pp.23-28
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• 2014

In order to increase the performance of conventional hot water floor heating system, the bubble jet loop heat pipe for the system was developed. This experiment was conducted under next conditions : Working fluid was R-134a, charging ratio was 50%. A temperature of hot water, room temperature and flow rate were $60^{\circ}C$, $15^{\circ}C$ and 0.5~1.5 kg/min, respectively. The experimental results, show that bubble jet loop heat pipe had a high effective thermal conductivity of $4714kW/m^{\circ}C$ and a sufficient heat flux of $73W/m^2$ to heat the floor to $35^{\circ}C$ in case of the 1.5 kg/min of flow rate. So the bubble jet loop heat pipe has a possibility for appling of the floor heating system. Additionally, the visualization of bubble jet loop heat pipe was performed to understand the operating principle. Bubbles made by the narrow gap between inner tube and outer tube of evaporating part generate pulsation at liquid surface of working fluid. The pulsation had slug flow and wavy flow. So working fluid circulates in the bubble jet loop heat pipe as two phase flow pattern. And large amount of heat is transferred by the latent heat from evaporating part to condensing part.

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.125-128
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• 2005

A 16S ribosomal RNA gene from S. chungbukensis DJ77 has been sequenced. This sequence had a length of 1,502 bp and was extended for 29 bp at 5' and for 37 bp at 3' from the partial sequence (1,435 bp) registered in 2000 year. Besides, 1 bp was newly added near to the 3' end. We made the secondary structure of the 16S rRNA based on E. coli model and found four specific regions. We found constant and variable regions in genus Sphingomonas as the result of multiple alignment of 16S rRNA gene sequences from Sphingomonas spp. and S. chungbukensis DJ77. We found a stem loop structure in S. chungbukensis DJ77, which was only discovered in C. jejuni to date. It showed the structural agreement despite the difference of the sequences from the both organisms. Finally, S. chungbukensis DJ77 belonged to cluster II (Sphingobium) group, after the classification using phylogenetic analysis and nucleotide signature analysis.

• Korean Chemical Engineering Research
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• v.55 no.3
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• pp.279-286
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• 2017

This review paper evaluates different kinds of synthesis methods for calcite precipitated calcium carbonates by using different materials. The various processing routes of calcite with different compositions are reported and the possible optimum conditions required to synthesize a desired particle sizes of calcite are predicted. This paper mainly focuses on that the calcite morphology and size of the particles by carbonation process using loop reactors. In this regard, we have investigated various parameters such as $CO_2$ flow rate, Ca $(OH)_2$ concentration, temperature, pH effect, reaction time and loop reactor mechanism with orifice diameter. The research results illustrate the formation of well-defined and pure calcite crystals with controlled crystal growth and particle size, without additives or organic solvents. The crystal growth and particle size can be controlled, and smaller sizes are obtained by decreasing the Ca $(OH)_2$ concentration and increasing the $CO_2$ flow rate at lower temperatures with suitable pH. The crystal structure of obtained calcite was characterized by using X-ray diffraction method and the morphology by scanning electron microscope (SEM). The result of x-ray diffraction recognized that the calcite phase of calcium carbonate was the dominating crystalline structure.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.11
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• pp.1571-1581
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• 1998

A flow visualization study was carried out for the branch pipe mixing flow in which a jet was issued normally to the fully developed pipe flow. An instantaneous laser tomographic method was used for cross flow Reynolds numbers based on the cross flow diameter D ranged $Re_{cf}=5.26{\times}10^3{\sim}1.13{\times}10^4$, diameter ratios d/D = 0.1 ~ 0.2 and velocity ratios R = 0.5 ~ 10. Oil mist with the size of about $10{\mu}m$ diameter was used for the scattering particle. The main purpose of this study was to reveal the physical mechanism and the structure of vortices formation with varying the velocity ratios and diameter ratios in the branch pipe flow. It was found that the physical mechanism and the structures of vortices formation were quite different depending on the velocity ratios. Particularly in the case of R < 1, the typical vortex shows single loop shape and that for the case of R > 1 depicts mushroom-like structure in the cross flow jet.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.12 no.12
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• pp.1081-1089
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• 2000

Evaporating heat transfer coefficients of R-22 and R-134a were measured in smooth horizontal copper tubes with inner diameters of 1.77, 3.36 and 5.35mm, respectively. The experiments were conducted in a closed loop, which was driven by a magnetic gear pump. Experiments were performed for the following range of variables: mass velocity (200 to 400 kg/$m^2$.s), saturation temperature($0^{circ}C,; 5^{\circ}C$) and quality(0 to 1.0). Main results obtained are as follows: evaporating heat transfer coefficients in the small diameter tubes (ID<7mm) were observed to be strongly affected by various diameters and to differ from those in the large diameter tubers. The heat transfer coefficients of the small diameter tubes were higher than those of the large diameter tubs. And it was very difficult to apply some well-known previous predictions (Shah`s, Gungor-Winterton`s and Kandlikar`s correlation) to small diameter tubes.

• Korean Journal of Microbiology
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• v.42 no.2
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• pp.156-159
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• 2006

A base substitution was introduced at the position 789 in Escherichia coli 16S rRNA, which was previously identified as an invariant residue for ribosome function and the ability of the mutant ribosomes to translate chloramphenicol acetyltransfernse mRNA was measured by determining the degree of resistance to chloramphenicol of cells expressing these mutant ribosomes. As expected, mutant ribosomes containing a base sub-stitution at the position 789 showed significantly reduced protein-synthesis ability and to identify a functional role played by this residue in protein synthesis, we developed an efficient genetic method to select second-site revertants in 16S rRNA that restore protein-synthesis function to these mutant ribosomes.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.1
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• pp.71-75
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• 2015

Nicotinic acetylcholine receptors (nAChRs) are essential for neurotransmission and important therapeutic targets of diseases related to neurotransmission. A recent experimental study identified three residues (Lys185, Asp187, and Ile188) of the ${\alpha}6$ nAChR subunit as determinants of ${\alpha}$-conotoxin BuIA selectivity, yet how these residues confer toxin selectivity remains unclear. In this study, we performed all-atom molecular dynamics simulations with two toxin-bound ${\alpha}4{\beta}2$ nAChR systems: the wild-type ${\alpha}4{\beta}2$ and one in which we replaced the three ${\alpha}4$ subunit residues with three ${\alpha}6$ subunit residues identified in an experimental study (Tyr185Lys, Thr187Asp, and Arg188Ile). After mutation, Asp199 lost the salt bridge formed with Arg188 in the wild type located around loop C. Then, the loop C conformation changed and became more flexible than that of the wild type. We also detected reduced space between the toxin and the binding site in the mutant simulation, resulting in increased binding affinity to the toxin. Therefore, we propose a new Asp199 mutation that breaks the salt bridge and may produce similar selectivity to that of the Arg188 mutation.

• Korean Journal of Veterinary Service
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• v.34 no.4
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• pp.333-339
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• 2011

Loop-mediated isothermal amplification (LAMP) was developed to detect Mycobacterium tuberculosis complex (MTC) and non-tuberculous mycobacterium (NTM) genomic DNA in blood samples of Korea native cattle. A set of four primers, two outer and two inner, were designed from M. bovis and M. avium genomic DNA targeting the IS6110 and 16S rRNA gene, respectively. Based on 85 Intradermal Tuberculin Test (ITT) positive blood sample and using conventional PCR and LAMP, the agreement quotient (kappa), which measures agreement beyond chance were 0.93 (conventional PCR) and 0.97 (LAMP), respectively. The detection limit of the LAMP method was $2.0{\times}10^2$ copy/ml M. bovis and M. avium cells, compared to $2.0{\times}10^3$ copy/ml M. bovis and M. avium cells for conventional PCR. These results suggest that the LAMP is a powerful tool for rapid, sensitive, and practical detection of MTC and NTM in blood samples of Korea native cattle.