Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Development of loop-mediated isothermal amplification method for the rapid and sensitive detection of bovine tuberculosis in Korea native cattle

한우 결핵의 신속 감별진단을 위한 등온증폭법 개발

  • 황은숙 (전북대학교 수의과대학 및 생체안전성연구소) ;
  • 이태욱 (전라남도축산위생사업소) ;
  • 정대영 (전라남도축산위생사업소) ;
  • 조호성 (전북대학교 수의과대학 및 생체안전성연구소)
  • Received : 2011.11.24
  • Accepted : 2011.12.05
  • Published : 2011.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Loop-mediated isothermal amplification (LAMP) was developed to detect Mycobacterium tuberculosis complex (MTC) and non-tuberculous mycobacterium (NTM) genomic DNA in blood samples of Korea native cattle. A set of four primers, two outer and two inner, were designed from M. bovis and M. avium genomic DNA targeting the IS6110 and 16S rRNA gene, respectively. Based on 85 Intradermal Tuberculin Test (ITT) positive blood sample and using conventional PCR and LAMP, the agreement quotient (kappa), which measures agreement beyond chance were 0.93 (conventional PCR) and 0.97 (LAMP), respectively. The detection limit of the LAMP method was $2.0{\times}10^2$ copy/ml M. bovis and M. avium cells, compared to $2.0{\times}10^3$ copy/ml M. bovis and M. avium cells for conventional PCR. These results suggest that the LAMP is a powerful tool for rapid, sensitive, and practical detection of MTC and NTM in blood samples of Korea native cattle.

Keywords

References

  1. 고바라다, 김현중, 박덕웅, 박성도, 김재익, 박종태, 김용환. 2007. PCR 기법을 이용한 도축 소의 결핵병 신속진단. 한국가축위생학회지 30: 393-406.
  2. 변현섭, 이현주, 이상명, 한성태, 곽학구, 최해연, 조윤상, 안병우. 2007. 도축 한우에서 발견된 결핵병. 한국가축위생학회지 30: 407-414.
  3. 신승원, 신민경, 차승빈, 우종태, 이성모, 구복경, 조윤상, 정석찬, 유한상. 2011. 국내 우군에서 소 결핵 진단을 위한 피내검사법과 Interferon-$\gamma$ 생성 검사의 비교. 대한수의학회지 51: 117-122.
  4. 유한상. 2007. 소 결핵병의 국제 연구 동향. 한국수의공중보건학회지 31: 69-82.
  5. Altman DG. 1999. Practical statistics for medical research. Chapman & Hall/CRC Press, Boca Raton/London/New York/Washington, DC.
  6. Biet F, Boschiroli ML, Thorel MF, Guilloteau LA. 2005. Zoonotic aspects of Mycobacterium bovis and Mycobacterium avium-intracellulare complex (MAC). Vet Res 36: 411-436. https://doi.org/10.1051/vetres:2005001
  7. Cho HS, Kang JI, Park NY. 2006a. Detection of canine parvovirus in fecal samples using loop-mediated isothermal amplification. J Vet Diagn Invest 18: 81-84. https://doi.org/10.1177/104063870601800111
  8. Cho HS, Kim YH, Park NY. 2006b. Disseminated mycobacteriosis due to Mycobacterium avium in captive Bengal tiger (Panthera tigris). J Vet Diagn Invest 18: 312-314. https://doi.org/10.1177/104063870601800318
  9. LoBue PA, Enarson DA, Thoen CO. 2010. Tuberculosis in humans and animals: an overview. Int J Tuberc Lung Dis 14: 1075-1078.
  10. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28: E63. https://doi.org/10.1093/nar/28.12.e63
  11. Ryoo SW, Shin S, Shim MS, Park YS, Lew WJ, Park SN, Park YK, Kang S. 2008. Spread of nontuberculous mycobacteria from 1993 to 2006 in Koreans. J Clin Lab Anal 22: 415-420. https://doi.org/10.1002/jcla.20278
  12. Schiller I, Oesch B, Vordermeier HM, Palmer MV, Harris BN, Orloski KA, Buddle BM, Thacker TC, Lyashchenko KP, Waters WR. 2010. Bovine tuberculosis: a review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transbound Emerg Dis 57: 205-220.
  13. Shin JB, Park NY, Kim YH, Cho HS. 2007. Dual priming oligonucleotide system for the multiplex detection of tuberculosis in Hanwoo. Kor J Vet Serv 30: 527-532.

Cited by

  1. Loop-mediated isothermal amplification assay for differentiation of Mycobacterium bovis and M. tuberculosis vol.36, pp.2, 2013, https://doi.org/10.7853/kjvs.2013.36.2.79
  2. Loop-mediated isothermal amplification assay for the rapid detection of swine influenza virus vol.38, pp.2, 2015, https://doi.org/10.7853/kjvs.2015.38.2.107
  3. Visual detection of porcine circovirus 2 by loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue dye vol.38, pp.3, 2015, https://doi.org/10.7853/kjvs.2015.38.3.145
  4. 구제역바이러스 신속진단을 위한 pan-serotype reverse transcription loop-mediated isothermal amplification (RT-LAMP) 진단법 vol.41, pp.1, 2011, https://doi.org/10.7853/kjvs.2018.41.1.29
  5. Mycobacterium genavense induced mycobacteriosis in an Indian peafowl (Pavo cristatus) vol.44, pp.2, 2011, https://doi.org/10.7853/kjvs.2021.44.2.119