Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Evaluation of bacteriophages for prevention and treatment of diarrhea due to experimental enterotoxigenic Escherichia coli K88 infection of weaned piglets

이유자돈의 실험적 enterotoxigenic Escherichia coli K88 감염에 의한 설사증에 대한 박테리오파지의 예방과 치료에 대한 효능 평가

  • Kim, Sung-Jae (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kim, Jin-Dong (Bio Business Unit, CJ ChilJedang Corp.) ;
  • Yang, Si-Yong (Bio Business Unit, CJ ChilJedang Corp.) ;
  • Kim, Nam-Hee (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Lee, Chang-Hee (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Yang, Don-Sik (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Han, Jeong-Hee (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 김성재 (강원대학교 수의과대학.동물종합연구소) ;
  • 김진동 (CJ 제일제당(주) 바이오사업부) ;
  • 양시용 (CJ 제일제당(주) 바이오사업부) ;
  • 김남희 (강원대학교 수의과대학.동물종합연구소) ;
  • 이창희 (강원대학교 수의과대학.동물종합연구소) ;
  • 양돈식 (강원대학교 수의과대학.동물종합연구소) ;
  • 한정희 (강원대학교 수의과대학.동물종합연구소)
  • Received : 2011.09.15
  • Accepted : 2011.11.08
  • Published : 2011.12.30
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Abstract

Colibacillosis in pigs remain a major swine industry bruden worldwide. Although some progress has been made in treating collibacillosis in pigs by using biosecurity and antimicrobials, it still remain a considerable problem. The use of host-specific bateriophages as a biocontrol is one possible alternative. The purpose of this study was to evaluate the effect of bacteriophage against enterotoxigenic Escherichia coli (ETEC) K88 infection in piglets. Twenty-eight piglets were randomly divided into four groups and each group was allocated with 7 pigs. Group B, C and D were inoculated with 5 ml of ETEC K88 ($1{\times}10^8$ CFU/ml) per head of piglet via oral. Group C and D were fed with bacteriophages (Group C, $1.0{\times}10^6$ PFU/g; Group D, $1.0{\times}10^8$ PFU/g; CJ CheilJedang Corp., Korea) orally as treatment. In piglets administrated bacteriophages and challenged with ETEC K88 (Group C and D), Clinical signs and the growth performance were improved and antibody titers were maintained low level compared with piglets challenged with ETEC K88 (Group B, P<0.05). Group B were shown high pH in the alimentary tracts compared with other piglets (P<0.05). In quantitative analysis by real-time PCR, the results of Group C and D were lower than those Group B in faecal and intestinal samples (P<0.05). Severe villus atrophy and crypt hyperplasia were observed in Group B consequently V/C ratio increased, compared with other piglets. These results indicate that feeding with bacteriophage has effect to prevent ETEC K88 infection in piglets and suggest that use of bacteriophage can be considered a valid antibiotic alternative.

Keywords

References

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