• Korean Journal of Veterinary Service
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• v.38 no.2
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• pp.107-116
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• 2015

In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that accidentally infected in pig population. The RT-LAMP was completed in 40 min at $58^{\circ}C$ and the sensitivity of the RT-LAMP ($1copy/{\mu}L$) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) ($10copy/{\mu}L$) and the same to real time RT-PCR ($1copy/{\mu}L$). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diagnostic laboratory in the future.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.65-65
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• 2012

The air texturing공정은 노즐에서 전달되는 초음속 에어기류에 의해 overfeed를 수반하여 yarn속 fiber가 뒤얽혀 loop와 crimp를 발달시키는데, 고강력 고탄성율 고내열성 내절단성 등의 특성을 가지는 아라미드섬유를 에어 가공사로 가공 할 경우 가공 전 필라멘트 상태일 때 보다 표면에 생기는 loop로 인하여 촉감이 좋아지고 또한 타 소재와 접착 시 접착제 담지 성능이 향상 되어 접착력이 상승되고, 이를 통해 보강재로서의 기능이 강화되는 반면 역학물성이 기존의 아라미드 보다 저하되는 약점을 가지고 있어 최근 ATY 공정조건이 ATY 사의 구조와 물성변화에 미치는 영향에 대한 많은 연구결과가 발표되고 있다. 본 연구에서는 Aiki air jet texturing machine에서 $Heracron^{(R)}$ para-aramid(840, 1000d, 1500d)를 사용하여 ATY nozzle의 직경을 0.6, 0.75, 1, 1.2mm로 변화를 주어 12개의 para-aramid ATY 시료를 제조하여 이들의 섬도, 강신도, 초기탄성률, 열수축률 그리고 형태불안정성(instability)등의 물성변화를 분석하여 다음과 같은 결과를 얻었다. 노즐의 직경이 증가함에 따라 사 내의 filament간의 움직임이 자유로워 교락이 증가하고 루프가 형성되어 단위길이 당 mass가 커지므로 섬도가 미세하게 증가하는 것을 볼 수 있다. 또한 직경이 증가할수록 절단강도와 초기탄성률은 감소하고 절단신도가 증가하는 경향을 볼 수 있는데 이는 축 방향으로의 배열이 적어져 하중을 분담하는 portion이 감소하고 사의 loop형성이 많아짐으로서 상대적으로 인장력에 대응하는 fiber의 수가 적어지기 때문으로 사료된다. 이는 현미경 관찰로 확인할 수 있는데 직경이 증가함에 따라 사의 loop의 엉킴이 증가하고 filament가 조밀한 것을 확인할 수 있다. 직경 변화에 따른 건 습열 수축률은 1% 미만의 매우 낮은 값으로 영향을 받지 않는 것을 확인 할 수 있는데 para-aramid의 열적특성의 안정성에 기인하는 것으로 사료되며 ATY의 불안정성은 노즐 직경 증가에 따른 어떤 경향성을 찾아볼 수 없었지만 840d, 1000d, 1500d로 섬도가 증가함에 따라 사의 불안정성이 증가하였다.

• Journal of the Korean Institute of Gas
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• v.12 no.1
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• pp.48-53
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• 2008

The condensation heat transfer coefficients of R-22 and R-410A in a small diameter tube were investigated. The main components of the refrigerant loop consist of a receiver, a variable-speed pump, a mass flowmeter, an evaporator (preheater), and a condenser (test section). The test section consists of smooth, horizontal copper tube of 3.38 mm outer diameter and 1.77 mm inner diameter. The refrigerant mass fluxes varied from 450 to $1050\;kg/(m^2s)$ and the average inlet and outlet qualities were 0.05 and 0.95. The main results were summarized as follows : the condensation heat transfer coefficient also increases with increasing mass flux and quality. The condensation heat transfer coefficient of R-410A was slightly higher than that of R-22. Most of correlations proposed in the large diameter tube showed significant deviations with experimental data except for the ranges of low quality and low mass flux.

• 전기의세계
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• v.29 no.9
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• pp.594-598
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• 1980

This paper describes an analytical method on the starting torque of hysteresis motor, taking account of penetration effects of magnetic-field and eddy-current into the rotor, to the elliptical approximation method of hysteresis-loop. By the above method, it have obtained the torque of rotor ring with non-magnetic and non-conductive material arbor, and the results are concerned and compared with that of computed by aid of callibration factor, k=1+exp(-2t$_{r}$/.delta.).)

• Journal of the Korean Society of Propulsion Engineers
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• v.19 no.2
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• pp.9-16
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• 2015

The control law for simultaneous pressure and thrust control of solid DACS(Divert Attitude Control System) is suggested. To regulate the two variables effectively, the control structure of sequential loop closer is applied to the system considering the physical characteristics of each variable and the weighted pseudo-inverse method is suggested to allocate effective command for indeterminate system. Also, the pressure guidance law for safe and high acceleration is applied to the homing stage to verify the effectiveness of the command distribution.

• Journal of the Korea Institute of Military Science and Technology
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• v.20 no.5
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• pp.673-680
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• 2017

In the proposed paper, we designed low noise frequency synthesizer with compact size for Multi-Band. The proposed frequency synthesizer consists of fundamental frequency band(2 GHz) and harmonic frequency band(4 GHz). To improve the phase noise and spurious level of frequency synthesizer, we analyze how the configuration of frequency synthesizer affect the phase noise and design the multi-band's structure. The implemented frequency synthesizer reduce both the phase noise and spurious level. The phase noise is -92.17 dBc/Hz at 1 kHz frequency offset in 2 GHz and -90.50 dBc/Hz at 1 kHz frequency offset in 4 GHz. All spurious signals including fundamental frequency are suppressed at least 20 dBc than the second harmonic frequency.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.10
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• pp.1044-1051
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• 2003

In this paper, a compact planar microwave duplexer on the high dielectric substrate is presented. As the Ba(Mg$\sub$1/3/Ta$\sub$2/3/)O$_3$(BMT) has good dielectric performances with a high dielectric constant of $\varepsilon$$\sub$r/=23, it is suitable to apply to a printed circuit board fur reducing its circuit size. The BMT substrate is fabricated by using a tape casting fabrication process, and circuit patterns are screen-printed on it by suing silver paste. The open-loop ring type duplexer is designed and implemented on the BMT substrate, and it achieves the smaller size by 80% than one on a commercial substrate($\varepsilon$$\sub$r/=6.15) without degenerating its performance. Therefore the proposed BMT substrate has provided the miniaturization of the duplexer, moreover it can make a contribution towards reducing the size of microwave passive circuits.

• Applied Microscopy
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• v.10 no.1_2
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• pp.7-17
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• 1980

Electron microscope radioautography introduced by Liquier-Milward (1956) is now used routinely in many laboratories. Most of the technical difficulties in specimen preparation have been overcome. This method is modified from loop method for improvement of EM radioautographic techniques. The advantages of this method are: 1. the use of single specimens on small corks and of a large wire loop, allows the experimenter to avoid the blemishes in the membrane; 2. the surfactant dioctyl sodium sulphosuccinate is added to diluted ILford L4, thus greatly prolonging the period of time over which good emulsion layers can be made; 3. corks can be handled in perspex holder which allows about 20 specimens to be developed simultaneously. The steps of the method comprise: 1. Cut ribbons of ultrathin sections of silver interference colour 2. Pick them up on formvar-coated 200 mesh grids 3. Prestaining of tissues 4. Coat the specimens with a thin layer of carbon by evaporation (30-60A) 5. Mount the specimens on corks (about 1cm apical diameter) using double-sided scotch tape 6. Emulsion coating; a. Take a 250m1 beaker, place it on the pan of a sliding weight balance and weigh it. Add 10 grams extra to the beam. Add pieces of ILford L4 emulsion to the beaker until the balance is swinging freely. Add the 20ml of distilled water that was previously measured out. b. Surfactant dioctyl sodium sulphosuccinate is added to diluted ILford L4. 7. Prepare a series of membranes of gelled emulsion with the wire loop and apply one to each cork-borne specimen. 8. Put the specimens away to expose by pushing the corks into short length of PVC tubing, each tube having a small hole in the side 9. Place the tubes in small boxes together with silica gel. 10. Exposure 11. Developer - Kodak Microdol X for 3 minutes 12. Fixer - A perspex holder can be manufactured which allows 20 specimens to be developed simultaneously. 12. Fixer - 30% sodium thiosulfate for 10 minutes 13. Examination with Siemens Elmiskop 1A electron microscope

• Journal of Microbiology and Biotechnology
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• v.29 no.2
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• pp.244-255
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• 2019

Xylose isomerase (XI; E.C. 5.3.1.5) catalyzes the isomerization of xylose to xylulose, which can be used to produce bioethanol through fermentation. Therefore, XI has recently gained attention as a key catalyst in the bioenergy industry. Here, we identified, purified, and characterized a XI (PbXI) from the psychrophilic soil microorganism, Paenibacillus sp. R4. Surprisingly, activity assay results showed that PbXI is not a cold-active enzyme, but displays optimal activity at $60^{\circ}C$. We solved the crystal structure of PbXI at $1.94-{\AA}$ resolution to investigate the origin of its thermostability. The PbXI structure shows a $({\beta}/{\alpha})_8$-barrel fold with tight tetrameric interactions and it has three divalent metal ions (CaI, CaII, and CaIII). Two metal ions (CaI and CaII) located in the active site are known to be involved in the enzymatic reaction. The third metal ion (CaIII), located near the ${\beta}4-{\alpha}6$ loop region, was newly identified and is thought to be important for the stability of PbXI. Compared with previously determined thermostable and mesophilic XI structures, the ${\beta}1-{\alpha}2$ loop structures near the substrate binding pocket of PbXI were remarkably different. Site-directed mutagenesis studies suggested that the flexible ${\beta}1-{\alpha}2$ loop region is essential for PbXI activity. Our findings provide valuable insights that can be applied in protein engineering to generate low-temperature purpose-specific XI enzymes.

• Journal of Electrical Engineering and Technology
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• v.5 no.2
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• pp.197-208
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• 2010

Unified power flow controller (UPFC) is the most reliable device in the FACTS concept. It has the ability to adjust all three control parameters effective in power flow and voltage stability. In this paper, a linearized model of a power system installed with a UPFC has been presented. UPFC has four control loops that by adding an extra signal to one of them, increases dynamic stability and load angle oscillations are damped. In this paper, after open loop eigenvalue (electro mechanical mode) calculations, state-space equations have been used to design damping controller and it has been considered to influence active and reactive power flow durations as the input of damping controller, in addition to the common speed duration of synchronous generators as input damper signal. To increase stability, further Lead-Lag and LQR controllers, a novel on-line adaptive controller has been used analytically to identify power system parameters. Closed-loop calculations of the electro mechanical mode verify the improvement of system pole placement after controller designing. Suitable operation of adaptive controller to decrease rotor speed oscillations against input mechanical torque disturbances is confirmed by the simulation results.