Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Loop-mediated isothermal amplification assay for the rapid detection of swine influenza virus

등온증폭법을 이용한 돼지인플루엔자바이러스 신속 진단법 개발

  • Kim, Eun-Mi (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Jeon, Hyo-Sung (Mmonitor Inc.) ;
  • Kim, Ji Jung (Mmonitor Inc.) ;
  • Kim, Hee-Jung (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Shin, Yeun-Kyung (Virology Division, Animal and Plant Quarantine Agency) ;
  • Song, Jae-Young (Virology Division, Animal and Plant Quarantine Agency) ;
  • Yeo, Sang-Geon (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Park, Choi-Kyu (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University)
  • 김은미 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 전효성 ((주)엠모니터) ;
  • 김지정 ((주)엠모니터) ;
  • 김희정 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 신연경 (농림축산검역본부 바이러스과) ;
  • 송재영 (농림축산검역본부 바이러스과) ;
  • 여상건 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 박최규 (경북대학교 수의과대학 & 수의전염병제어센터)
  • Received : 2015.05.19
  • Accepted : 2015.06.22
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that accidentally infected in pig population. The RT-LAMP was completed in 40 min at $58^{\circ}C$ and the sensitivity of the RT-LAMP ($1copy/{\mu}L$) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) ($10copy/{\mu}L$) and the same to real time RT-PCR ($1copy/{\mu}L$). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diagnostic laboratory in the future.

Keywords

References

  1. Chander Y, Koelbl J, Puckett J, Moser MJ, Klingele AJ, Liles MR, Carrias A, Mead DA, Schoenfeld TW. 2014. A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification (LAMP). Front Microbiol 5: 395.
  2. Cho AR, Dong HJ, Cho SB. 2013. Rapid and Sensitive Detection of Salmonella spp. by Using a Loop-Mediated Isothermal Amplification Assay in Duck Carcass Sample. Korean J. Food Sci. An 33: 655-663. https://doi.org/10.5851/kosfa.2013.33.5.655
  3. Cho HS and Park NY. 2005. Detection of canine distemper virus in blood samples by reverse transcription loop-mediated isothermal amplification. J Vet Med B 52: 410-413. https://doi.org/10.1111/j.1439-0450.2005.00886.x
  4. Cong YL, Pu J, Liu QF, Wang S, Zhang GZ, Zhang XL, Fan WX, Brown EG, Liu JH. 2007. Antigenic and genetic characterization of H9N2 swine influenza viruses in China. J Gen Virol 88: 2035-2041. https://doi.org/10.1099/vir.0.82783-0
  5. Dhama K, Karthik K, Chakraborty S, Tiwari R, Kapoor S, Kumar A, Thomas P. 2014. Loop-mediated isothermal amplification of DNA (LAMP): a new diagnostic tool lights the world of diagnosis of animal and human pathogens: a review. Pak J Biol Sci 17: 151-166. https://doi.org/10.3923/pjbs.2014.151.166
  6. Dong HJ, Cho AR, Hahn TW. 2014. Seongbeom Cho. Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle. J Vet Sci 15: 317-325. https://doi.org/10.4142/jvs.2014.15.2.317
  7. Gandelman O, Jackson R, Kiddle G, Tisi L. 2011. Loop-mediated amplification accelerated by stem primers. Int J Mol Sci 12: 9108-9124. https://doi.org/10.3390/ijms12129108
  8. Gu H, Qi X, Li X, Jiang H, Wang Y, Liu F, Lu S, Yang Y, Liu F. 2010. Rapid and specific detection of H3 swine influenza virus using reverse transcription loop-mediated isothermal amplification method. J Applied Microbiol 108: 1145-1154. https://doi.org/10.1111/j.1365-2672.2009.04520.x
  9. Hass J, Matuszewski S, Cieslik D, Haase M. 2011. The role of swine as "mixing vessel" for interspecies transmission of he influenza A subtype H1N1: a simultaneous Bayesian inference of phylogeny and ancestral hosts. Infect Genet Evol 11: 437-441. https://doi.org/10.1016/j.meegid.2010.12.001
  10. Hoffmann, E, Stech J, Guan Y, Webster RG, and Perez DR. 2001. Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol 146: 2275-2289. https://doi.org/10.1007/s007050170002
  11. Hwang ES, Lee TU, Jung EY, Cho HS. 2011. Development of loop-mediated isothermal amplification method for the rapid and sensitive detection of bovine tuberculosis in Korea native cattle. Korean J Vet Serv 34: 333-339. https://doi.org/10.7853/kjvs.2011.34.4.333
  12. Karasin AI, Anderson GA, Olsen CW. 2000. Isolation and characterization of H4N6 avian influenza viruses from pigs with pneumonia in Canada. J Virol 74: 9322-9327. https://doi.org/10.1128/JVI.74.19.9322-9327.2000
  13. Karasin AI, West K, Carman S, Olsen CW. 2004. Characterization of avian H3N3 and H1N1 influenza A viruses isolated from pigs in Canada. J Clin Microbiol 42: 4349-4354. https://doi.org/10.1128/JCM.42.9.4349-4354.2004
  14. Kim HR, Oem JK, Bae YC, Kang MS, Lee HS, Kwon YK. 2013. Application of real-time reverse transcription polymerase chain reaction to the detection the matrix, H5 and H7 genes of avian influenza viruses in field samples from South Korea. Virol J 10: 85. https://doi.org/10.1186/1743-422X-10-85
  15. Kim SH, Moon OK, Lee KK, Song YK, Yeo CI, Bae CW, Yoon H, Lee OS, Lee JH, Park CK. 2011. Outbreak of pandemic influenza (H1N1) 2009 in pigs in Korea. Vet Rec 169: 155a.
  16. Kim SH, Roh IS, Lee KK, Park CK. 2014. A novel reassortant H1N2 virus related to the pandemic H1N1 2009 influenza virus isolated from Korean pigs. Virus Genes 48: 193-198. https://doi.org/10.1007/s11262-013-0996-x
  17. Koh BRD, Kim JM, Sung CM, Ji TK, Na HM, Park SD, Kim YH, Kim ES. 2013. Loop-mediated isothermal amplification assay for differentiation of Mycobacterium bovis and M. tuberculosis. Korean J Vet Serv 36: 79-86. https://doi.org/10.7853/kjvs.2013.36.2.79
  18. Lekcharoensuk P, Lager KM, Vemulapalli R, Woodruff M, Vincent AL, Richt JA. 2006. Novel swine influenza virus subtype H3N1, United States. Emerg Infect Dis 12: 787-794. https://doi.org/10.3201/eid1205.051060
  19. Lee MS, Chang PC, Shien JH, Cheng MC, Shieh HK. 2001. Identification and subtyping of avian influenza viruses by reverse transcription-PCR. J Virol Methods 97: 13-22. https://doi.org/10.1016/S0166-0934(01)00301-9
  20. Liu Y, Jiang J, Zhang L, Chen L and Ning Y. 2012. Development and application of a rapid detection system for swine influenza virus by a reverse transcription loop-mediated isothermal amplification assay. J Food Agri Environ 10: 196-200.
  21. Ma XJ, Shu YL, Nie K, Qin M, Wang DY, Gao RB, Wang M, Wen LY, Han F, Zhou SM, Zhao X, Cheng YH, Li DX, Dong XP. 2010. Visual detection of pandemic influenza A H1N1 virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye. J Virol Methods 167: 214-217. https://doi.org/10.1016/j.jviromet.2010.03.027
  22. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino and N, Hase T. 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28: 63-69. https://doi.org/10.1093/nar/28.12.e63
  23. OIE. 2012. Manual of diagnostic test and Vaccines for terrestrial Animals. http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.03.04_AI.pdf
  24. Parida M, Shukla J, Sharma S, Santhosh SR, Ravi V, Mani R, Thomas M, Khare S, Rai A, Ratho RK, Pujari S, Mishra B, Rao PVL, Vijayaraghavan R. 2011. Development and evaluation of reverse transcription loop-mediated isothermal amplification assay for rapid and real-time detection of the swine-origin influenza A H1N1 virus. J Mol Diagn 13: 100-107. https://doi.org/10.1016/j.jmoldx.2010.11.003
  25. Poon LLM, Leung CSW, Chan KH, Lee JHC, Yuen KY, Guan Y, and Peiris JSM. 2005 Detection of human influenza A viruses by loop-mediated isothermal amplification. J Clin Microbiol 43: 427-430. https://doi.org/10.1128/JCM.43.1.427-430.2005
  26. Phipps LP, Essen SC, I.H. Brown IH. 2004. Genetic subtyping of influenza A viruses using RT-PCR with a single set of primers based on conserved sequences within the HA2 coding region. J Virol Methods 122: 119-122. https://doi.org/10.1016/j.jviromet.2004.08.008
  27. Sakurai A and Shibasaki F. 2012. Updated values for molecular diagnosis for highly pathogenic avian influenza virus. Viruses 4: 1235-1257. https://doi.org/10.3390/v4081235
  28. Shin YK, Yoon SS, Song JY, Kim JB, Hang DT, Park JW, Kim MY, Kim SH, Park CK, Li OS. 2011. One-step multiplex reverse-transcriptase PCR for detecting pandemic (H1N1) 2009 influenza virus. J Vet Med Sc 73: 55-63. https://doi.org/10.1292/jvms.10-0218
  29. Van Reeth K, Brown IH and Olsen CW. 2012. Influenza virus. pp. 557-571. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ and Stevenson GW (ed.). Diseases of Swine, 10th ed. Wiley-Blackwell Publishing, Ames, Iowa.
  30. Vincent A, Awada L, Brown I, Chen H, Claes F, Dauphin G, Donis R, Culhane M, Hamilton K, Lewis N, Mumford E, Nguyen T, Parchariyanon S, Pasick J, Pavade G, Pereda A, Peiris M, Saito T, Swenson S, Van Reeth K, Webby R, Wong F, Ciacci-Zanella J. 2014. Review of influenza A virus in swine worldwide: a call for increased surveillance and research. Zoonoses Public Health 61: 4-17. https://doi.org/10.1111/zph.12049
  31. Vijaykrishna D, Poon LL, Zhu HC, Ma SK, Li OT, Cheung CL, Smith GJ, Peiris JS, Guan Y. 2010. Reassortment of pandemic H1N1/2009 influenza A virus in swine. Science 328: 1529. https://doi.org/10.1126/science.1189132
  32. WHO. 2010. Pandemic (H1N1)2009-update100. http://www.who.int/csr/don/2010_05_14/en/index.html
  33. Yoo MS, Noh JH, Yoon BS, Reddy KE, Kweon CH, Jung SC, Kang SW. 2012. Reverse transcription loop-mediated isothermal amplification for sensitive and rapid detection of Korean sacbrood virus. J Virol Methods 186: 147-151. https://doi.org/10.1016/j.jviromet.2012.08.009

Cited by

  1. Visual detection of porcine circovirus 2 by loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue dye vol.38, pp.3, 2015, https://doi.org/10.7853/kjvs.2015.38.3.145
  2. Uracil-DNA glycosylase-treated reverse transcription loop-mediated isothermal amplification for rapid detection of avian influenza virus preventing carry-over contamination vol.17, pp.3, 2016, https://doi.org/10.4142/jvs.2016.17.3.421
  3. 구제역바이러스 신속진단을 위한 pan-serotype reverse transcription loop-mediated isothermal amplification (RT-LAMP) 진단법 vol.41, pp.1, 2015, https://doi.org/10.7853/kjvs.2018.41.1.29
  4. 국화에 발생하는 반쪽시들음병균 Verticillium dahliae 검출용 등온 증폭법 개발 vol.47, pp.4, 2015, https://doi.org/10.4489/kjm.20190048