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Measurement of Microbial Protein Supply in Murrah Buffaloes (Bubalus bubalis) Using Urinary Purine Derivatives Excretion and PDC Index

  • Dipu, M.T.;George, S.K.;Singh, P.;Verma, A.K.;Mehra, U.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.3
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    • pp.347-355
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    • 2006
  • A study was conducted to predict the rumen microbial protein production based on urinary excretion of purine derivatives in buffaloes fed a diet of wheat straw and concentrate (40:60) at four fixed levels of feed intake. (95, 80, 60 and 40% of preliminary voluntary feed intake) following experimental protocol of IAEA (Phase I). The buffaloes were allocated according to a $4{\times}4$ latin square design. The urinary allantoin, uric acid, total PD excretion (mmol/d) in treatments L-95, L-80, L-60 and L-40 was 20.13, 16.00, 12.96 and 9.17; 1.88, 2.12, 2.11 and 2.15; 22.01, 18.12, 15.07 and 11.32, respectively and were significantly (p<0.05) different among treatments except for uric acid. The rate of PD excretion (mmol/d) was positively correlated with the digestible organic matter intake. Variations were observed in PD and creatinine concentration in spot samples collected at 6-hour interval. However, daily PD:Creatinine ratio (PDC index) appears to be a reasonably good predictor of microbial-N supply. The contribution of basal purine excretion to total excretion of purine derivatives (PD) was determined in pre-fasting period followed by a fasting period of 6 d (Phase II). Daily PD and creatinine excretion (mmol/kg $W^{0.75}$) during fasting averaged 0.117 and 0.456 respectively for buffaloes. The excretion rates of PD decreased significantly (p<0.01) during fasting compare to pre-fasting period, the urinary creatinine excretion remained almost similar. Except for creatinine, plasma concentration of target parameters significantly (p<0.01) declined during fasting. Likewise, glomerular filtration rate (GFR) and renal clearance of allantoin and uric acid also decreased. Based on the PD excretion rates during fasting and at different levels of feed intake obtained in this study, a relationship between daily urinary PD excretion (Y-mmol) and microbial purine absorption (X-mmol) was developed for buffaloes as Y = 0.74X+0.117 kg $W^{0.75}$. The microbial N supply (g/kg DOMI) remained statistically similar irrespective of dietary treatment. The results showed that excretion of urinary purine derivatives is positively correlated with the levels of feed intake in Murrah buffaloes and thus, estimation of urinary purine derivatives and PDC index could be used to determine microbial nitrogen supply when there is large variation in level of feed intake.

Evaluation of Binder jetting 3D Printed Specimens Using Vacuum Impregnation (진공함침을 적용한 바인더젯 3D 프린팅 출력물의 성능 평가)

  • Park, Kwang-Min;Park, Su-Hyeon;Lee, Jun;Lee, Bong-Chun;Roh, Young-Sook
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.24 no.2
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    • pp.103-110
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    • 2020
  • This study examined the applicability of the vacuum impregnation post-processing to enhance the strength of binder jet 3D printed output. In addition, permeability, bulk density, and compressive strength of 10 mm, 20 mm, 30 mm, and 40 mm cubic specimens were examined to check the strength limit depending on the 3D prined output size. In result, as the maximum pressure increased, the post-processing storage solution permeated to the inside of the 3D printed sample and thus the permeation area ratio was improved. The compressive strength and the permeation area indicate the correlation between the exponential function of the adjusted R-square factor 0.992. In addition, the bulk density was increased, which can be inferred as the post-processing solution permeated to the inside. In conclusion, in order to enhance the compressive strength of the binder jet 3D printed output, it is essential to permeate the post-processing solution to the inside of the output, and vacuum impregnation can be proposed as an effective method.

A STUDY ON THE THERMAL DIFFUSION THROUGH VARIOUS THICKNESS OF BASE AND RESTORATION MATERIALS (수복물(修復物)과 양장재(襄裝材)의 열확산(熱擴散)에 관(關)한 연구(硏究))

  • Yoon, Dong-Ho;Park, Sang-Jin;Min, Byung-Soon;Choi, Ho-Young
    • Restorative Dentistry and Endodontics
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    • v.11 no.1
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    • pp.77-88
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    • 1985
  • The purpose of this study was to examine the thermal diffusion through bases and restorations. The three principle types of base and two restorative materials were included in this study. They were representive brands of a zinc phosphate cement, a zinc oxide-eugenol cement, a calcium hydroxide paste, an amalgam and a composite resin (table 1). The specimens were prepared by placing the bases or restorative materials in laminated plastic molds. 5-mm diameter holes were prepared in the center of square of plastics which were 0.5, 1.0, 2.0, and 3.0mm thick respectively (fig. 1). All materials were manipulated in accordance with manufacturer's recommended proportions. All experimental procedures were carried out dividing them into eight different groups (table 2). Thermal diffusion was measured by means of digital thermometer (DP-100, RKC. instrument Inc. JAPAN) with the surface thermocouple placed on bottom surface of the specimen applying a constant source of heat and cold to the top surface of the each specimen. The thermal stimulus temperature applied on the each specimen surface was in the range of $60^{\circ}C$, $0^{\circ}C$ and $-50^{\circ}C$ respectively. The thermal change were recorded automatically on the multi-Pen recorder (R-16, Rikadenki, Co. JAPAN) connected with thermocouple tips which were centered on the bottom of the specimen. The following results were as follows, 1. Temperature diffusion was highest through amalgam and slowest through the composite resin. 2. As the thickness of restorations increased, the temperature change was decreased. 3. Thermal diffusion was slowest in the presence of zinc oxide-eugenol bases, followed by calcium hydroxide and zinc phosphate cement. 4. The efficiency of the cement bases in providing thermal insulation was dependent on their thickness beneath the restorations. 5. Thermal change was great in the range of $60^{\circ}C$ and $-50^{\circ}C$, but little in the range of $0^{\circ}C$.

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Clinical study of Bangpoongtongsungsan on body weight change in subjects with obesity (방풍통성산이 비만인에 미치는 영향에 대한 임상적 연구)

  • Shin, Dae-Hee;Cho, Guk-Hyun;Lee, Hyuck;Moon, Mi-Kyung;Kang, Dae-Gill;Yun, Young-Gab;Park, Do-Shim;Juhng, Seon-Kwan;Lee, Ho-Sub
    • Herbal Formula Science
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    • v.16 no.2
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    • pp.133-144
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    • 2008
  • Recently, people are becoming fatter in most parts of the world. Obesity is regarded as an important risk factor to adversely affect the health of humans, associated with hypertension, hyperlipidemia, diabetes, and cardiovascular diseases. In the present study, therefore, we tested the short time efficiency and safety of Bangpoongtongsungsan as a drug to decrease body weight in subjects with obesity. A total 48 volunteers (24 man, 24 woman) with body mass index (BMI : weight in kg divided by square of height in meters) of $23㎏/m^2$ participated in clinical study for 12 weeks, from 2008, March 01 to 2008, Jun 30. The subjects was received a dietary supplement of 12 pieces hard capsules per day (4 pieces in one, 3 times in a day) during the clinical study. At the end of clinical study, body weight of subjects was significantly decreased from $72.21{\pm}11.44\;kg$ to $70.53{\pm}11.67\;kg$ (p<0.05). Waist circumferences of subjects were reduced $91.58{\pm}6.43\;cm$ to $85.25{\pm}7.70\;cm$ (p<0.05). Also, a BMI of subjects was significantly decreased $26.07{\pm}2.35\;kg/m^2$ to $25.63{\pm}2.63\;kg/m^2$ (p<0.05). Particularly, a decrease in body weight, waist circumferences, and BMI was more significant in woman than man. But, other biochemical levels including lipid and glucose in plasma had no changes at the end of clinical study. In conclusion, administration of Bangpoongtongsungsan in subjects with high body weight fat could inhibit obesity associated with a decrease in body weight, waist circumferences, and BMI.

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Analysis of the Characteristics of Subway Influence Areas Using a Geographically Weighted Regression Model (지리가중회귀모델을 이용한 역세권 공간구조 특성 분석)

  • Sim, Jun-Seok;Kim, Ho-Yong;Nam, Kwang-Woo;Lee, Sung-Ho
    • Journal of the Korean Association of Geographic Information Studies
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    • v.16 no.1
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    • pp.67-79
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    • 2013
  • For the sake of the Transit-Oriented Development that has been prominent recently, an analysis of the spatial structures of transit centers, above all, should be carried out at a local level. This study, thus, analyzes the spatial structures of subway influence areas by applying a Geographically Weighted Regression (GWR) model to individual parcels. As a result of the validity analysis of the model, it has turned out that the subway influence areas have different characteristics respectively, and there is spatial heterogeneity even in the same single area. Also, the result of the comparison among models has proved that the GWR model is more adequate than the Ordinary Least Square (OLS) model and $R^2$ has been also increased in the GWR model. Then, the results have been mapped by means of the GIS, which have made it possible to understand the spatial structures at a local level. If the Transit-Oriented Development is fulfilled in consideration of the spatial structural characteristics of the subway influence areas drawn respectively from the model analysis, it will be helpful in adopting effective policies.

Prediction of Crude Protein, Extractable Fat, Calcium and Phosphorus Contents of Broiler Chicken Carcasses Using Near-infrared Reflectance Spectroscopy

  • Kadim, I.T.;Mahgoub, O.;Al-Marzooqi, W.;Annamalai, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.1036-1040
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    • 2005
  • Near-infrared reflectance spectroscopic (NIRS) calibrations were developed for accurate and fast prediction of whole broiler chicken carcass composition. The Feed and Forage Foss systems Model 5000 Reflectance Transport Model 5000 with near-infrared reflectance spectroscopy (NIRS)-WinISI II windows software was used for this purpose. One equation was developed for the prediction of each carcass component. One hundred and fifty freeze dried broiler whole carcass samples were ground in a Cyclotech 1,093 sample mill and analyzed for dry matter, protein, fat, calcium and phosphate. Samples were divided into two sets: a calibration set from which equations were derived and a prediction set used to validate these equations. The chemical analysis values (mean${\pm}$SD) were calculated based on dry matter basis as follows: dry matter: 33.41${\pm}$2.78 (range: 26.41-43.47), protein: 54.04${\pm}$6.63 (range: 36.20-76.09), fat 35.44${\pm}$8.34 (range: 7.50-55.03), calcium 2.55${\pm}$0.65 (range: 0.99-4.41), phosphorus 1.38${\pm}$0.26 (range: 0.60-2.28). One hundred and three samples were used to calibrate the equations and prediction values. The software used was modified to obtain partial least square regression statistics, as it is the most suitable for natural products analysis. The coefficients of determination ($R^2$) and the standard errors of prediction were 0.82 and 1.83 for the dry matter, 0.96 and 1.98 for protein, 0.99 and 1.07 for fat, 0.90 and 0.30 for calcium and 0.91 and 0.11 for phosphorus, respectively. The present study indicated that NIRS can be calibrated to predict the whole broiler carcass chemical composition, including minerals in a rapid, accurate, and cost effective manner. It neither requires skilled operators nor generates hazardous waste. These findings may have practical importance to improve instrumental procedures for quick evaluation of broiler carcass composition.

A Study on Energy-saving Landscape Facilities through a Park User's Attitude Survey - Focused on Urban Parks in Gimpo - (공원이용자 의식조사를 통한 에너지 절약형 조경시설물 도입에 대한 연구 - 김포시 근린공원을 중심으로 -)

  • Doo, Chul-Eon;Lee, Jae-Keun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.15 no.3
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    • pp.17-28
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    • 2012
  • This study aims to present the ground for the introduction of energy-saving landscape facilities which can meet the requirements of the age and create a new space by introducing the facilities which saves energy and that users prefer at the space where urbanites enjoy leisure activities in the urban parks. Thus, in addition to a spatial analysis of the parks for 10 urban parks in Gimpo, through an attitude survey on the users' behaviors, the following conclusions were drawn out. First, as a result of putting all facilities in the urban parks in Gimpo, on average, 6.9 out of the standards of 9 (76.6%) have been installed, and as a result of an analysis on the total number of the installed facilities, on average, about 28 kinds of facilities have been installed, so only 22.4% of the total 125 facilities were installed. Second, many urbanites are using the parks for the purpose of taking a walk and exercise to improve their health; however, the legal standards regulate only 2.9 exercise facilities on average in the target area, which are somewhat insufficient. Third, regarding the citizens' intent to participate in responding to climate change, 76% of them showed their intention of participation. Thus, if the energy-saving facilities, the purpose of this study, are introduced, many users will use them, and it is judged that costs for existing street lights and electric power plants will decrease. Fourth, as a result of an analysis of their satisfaction with the facilities, the value of R-square meaning the explanatory power of the independent variables put in a linear model for dependent variables was 0.860, approximately 81% of the total satisfaction, which was a high explanatory power. This study conducted an attitude survey on the users of urban parks in Gimpo at the dimensions of the introduction of energy-saving facilities in the urban parks, the national policies and responses to climate change. It is significant that it identified the appropriateness of developing much more energy-saving landscape facilities needed for low-carbon green growth in various types, and as a subsequent project, a more precision study on this is necessary continuously.

Agronomic and Silage Traits of Corn over Time (수확시기에 따른 사일리지용 옥수수의 생육특성 및 수량 비교)

  • Chung, Eui-Soo;Jo, Mu-Hwan;Kim, Jong-Keun;Nam, Deuk-Woo;Jin, Sung-Joo;Jang, Suek-Yoon;Kang, Hee-Seol
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.4
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    • pp.325-332
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    • 2010
  • Pioneer 31N27 variety was planted on April 19 at Suwon to determine agronomic and silage traits over time. Ten harvest times was made at intervals of 10 days beginning July 1 to September 20, 2010. Stalk dry matter (DM) content increased with maturity, the highest by 58.5% on September 10 and then decreased (p<0.05). Ear and whole-plant DM content increased linearly with maturity, and whole-plant DM reached by 30% on August 11. Ear DM to whole-plant DM increased with maturity and reached by 63.2% on September 20. Ear DM yield increased with maturity, and stalk, whole-plant and TDN yield were the highest on September 10 and then decreased (p < 0.05). A regression coefficient for DM yield with maturity were 0.57 for stalk, 0.92 for ear, 0.90 for whole-plant and 0.93 for TDN yield.

Development and Validation of Predictive Models of Esherichia coli O157:H7 Growth in Paprika (파프리카에서 병원성 대장균의 성장예측 모델 개발 및 검증)

  • Yun, Hyejeong;Kim, Juhui;Park, Kyeonghun;Ryu, Kyoung-Yul;Kim, Byung Seok
    • Journal of Food Hygiene and Safety
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    • v.28 no.2
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    • pp.168-173
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    • 2013
  • This study was carried out to develop and validate predictive models of E. coli O157:H7 growth. Growth data of E. coli O157:H7 in Paprika were collected at 12, 24, 30 and $36^{\circ}C$. The population increased into 3.0 to 3.8 log10 CFU/g within 4 days, then continued to increase at a slower rate through 10 days of storage at $12^{\circ}C$. The lag time (LT) and maximum specific growth rate (SGR) obtained from each primary model was then modeled as a function of temperature using Davey and square root equations, respectively. For interpolation of performance evaluation, growth data for a mixture of E. coli O157:H7 were collected at time intervals in paprika incubated at the different temperatures, which was not used in model development. Results of model performance for interpolation data demonstrated that induced secondary models showed acceptable goodness of fit. Relative errors in the LT and SGR model for interpolation data (18 and $27^{\circ}C$) was 100%, which show acceptable goodness of fit and validated for interpolation. The primary and secondary models developed in this study can be used to establish tertiary models to quantify the effects of temperature on the growth of E. coli O157:H7 in paprika.

Estimation of Stand-level Above Ground Biomass in Intact Tropical Rain Forests of Brunei using Airborne LiDAR data (항공 LiDAR 자료를 이용한 브루나이 열대우림의 임분단위 지상부 생체량 추정)

  • Yoon, Mihae;Kim, Eunji;Kwak, Doo-Ahn;Lee, Woo-Kyun;Lee, Jong-Yeol;Kim, Moon-Il;Lee, Sohye;Son, Yowhan;Salim, Kamariah Abu
    • Korean Journal of Remote Sensing
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    • v.31 no.2
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    • pp.127-136
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    • 2015
  • This study aims to quantify the stand-level above ground biomass in intact tropical rain forest of Brunei using airborne LiDAR data. Twenty four sub-plots with the size of 0.09ha ($30m{\times}30m$) were located in the 25ha study area along the altitudinal gradients. Field investigated data (Diameter at Breast Height (DBH) and individual tree position data) in sub-plots were used. Digital Surface Model (DSM), Digital Terrain Model (DTM) and Canopy Height Model (CHM) were constructed using airborne LiDAR data. CHM was divided into 24 sub-plots and 12 LiDAR height metrics were built. Multiple regression equation between the variables extracted from the LiDAR data and biomass calculated by using a allometric equation was derived. Stand-level biomass estimated from LiDAR data were distributed from 155.81 Mg/ha to 597.21 Mg/ha with the mean value of 366.48 Mg/ha. R-square value of the verification analysis was 0.84.