• Title/Summary/Keyword: R-loop

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Genetic Diversity and Phylogenetic Analysis of South-East Asian Duck Populations Based on the mtDNA D-loop Sequences

  • Sultana, H.;Seo, D.W.;Bhuiyan, M.S.A.;Choi, N.R.;Hoque, M.R.;Heo, K.N.;Lee, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.12
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    • pp.1688-1695
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    • 2016
  • The maternally inherited mitochondrial DNA (mtDNA) D-loop region is widely used for exploring genetic relationships and for investigating the origin of various animal species. Currently, domestic ducks play an important role in animal protein supply. In this study, partial mtDNA D-loop sequences were obtained from 145 samples belonging to six South-East Asian duck populations and commercial duck population. All these populations were closely related to the mallard duck (Anas platyrhynchos), as indicated by their mean overall genetic distance. Sixteen nucleotide substitutions were identified in sequence analyses allowing the distinction of 28 haplotypes. Around 42.76% of the duck sequences were classified as Hap_02, which completely matched with Anas platyrhynchos duck species. The neighbor-joining phylogenetic tree also revealed that South-East Asian duck populations were closely related to Anas platyrhynchos. Network profiles were also traced using the 28 haplotypes. Overall, results showed that those duck populations D-loop haplotypes were shared between several duck breeds from Korea and Bangladesh sub continental regions. Therefore, these results confirmed that South-East Asian domestic duck populations have been domesticated from Anas platyrhynchos duck as the maternal origins.

The Relationship of Structural Properties, Subjective Textures and Sensibilities of Knit Fabrics - Wool/Rayon Fiber Contents and Loop Length - (니트 소재의 구성 특성과 주관적 질감 및 감성의 관계 - 양모/레이온 혼용률 및 편환장 변화를 중심으로 -)

  • Ju, Jeong-Ah;Ryu, Hyo-Seon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.8 s.145
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    • pp.1158-1167
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    • 2005
  • The purpose of this study is to examine the effect of structural properties of plain knit fabrics on the subjective textures and sensibilities of consumers. We investigated the relationship of subjective textures and sensibilities according to fiber contents of wool/rayon and stitch loop length. We made 20 plain knit fabrics, as specimens, with a combination of 5 kinds of fiber contents and 4 kinds of stitch loop length. A factor analysis showed that subjective textures are classified into 3 categories with $R^2=70.32\%$: 'surface-rough', 'drapable', 'bulky' and 'elastic' and subjective sensibilities into 3 categories with $R^2=68.12\%$: 'stable/neat', 'feminine/elegant' and 'natural/comfortable'. The fiber contents of wool/rayon had a positive and linear relation with 'surface-rough', but had a relatively non-linear one with 'bulky' and 'elastic' categories of textures, and 'feminine/elegant' of sensibilities. The stitch loop length had a linear influence on 'drapable' and 'stable/neat', but had a non-linear influence on other subjective textures and sensibilities.

The Low Height Looping Technology for Multi-chip Package in Wire Bonder (와이어 본더에서의 초저 루프 기술)

  • Kwak, Byung-Kil;Park, Young-Min;Kook, Sung-June
    • Journal of the Semiconductor & Display Technology
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    • v.6 no.1 s.18
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    • pp.17-22
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    • 2007
  • Recent new packages such as MCP(Multi-Chip Package), QDP(Quadratic Die Package) and DDP(Dual Die Package) have stack type configuration. This kind of multi-layer package is thicker than single layer package. So there is need for the low height looping technology in wirebonder to make these packages thinner. There is stiff zone above ball in wirebonder wire which is called HAZ(Heat Affect Zone). When making low height loop (below $80\;{\mu}m$) with traditional forward loop, stiff wire in HAZ(Heat Affected Zone) above ball is bended and weakened. So the traditional forward looping method cannot be applied to low height loop. SSB(stand-off stitch) wire bonding method was applied to many packages which require very low loops. The drawback of SSB method is making frequent errors at making ball, neck damage above ball on lead and the weakness of ball bonding on lead. The alternative looping method is BNL(ball neckless) looping technology which is already applied to some package(DDP, QDP). The advantage of this method is faster in bonding process and making little errors in wire bonding compared with SSB method. This paper presents the result of BNL looping technology applied in assembly house and several issues related to low loop height consistence and BNL zone weakness.

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Determination of the MYB Motif Interacting with WD40 and Basic Helix Loop Helix Proteins

  • Kim, Ji-Hye;Kim, Bong-Gyu;Ahn, Joong-Hoon
    • Journal of Applied Biological Chemistry
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    • v.55 no.1
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    • pp.67-70
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    • 2012
  • Plant MYB transcription factors regulate secondary metabolism, cellular morphogenesis, and plant hormone signaling pathway. MYB proteins in plants consist of two repeats of 50 amino acid residues, which are referred to as R2R3 and they interact with WD40 or basic helix loop helix (bHLH) proteins. Yeast two hybrid assay was determined whether rice MYB protein interacts with either OsTTG1, which contains a WD40 domain, or with OsGL3, which contains a bHLH domain. Among 30 OsMYB proteins, three interacted with OsTTG1 and five interacted with OsGL3. A series of MYB mutants were created to determine the MYB domain important for the interaction with OsTTG1 or OsGL3. By using the yeast two hybrid assay, we found that the R3 motif of OsMYB10 and the R2 motif of OsMYB16 were required for interaction with OsTTG1 and OsGL3 proteins, respectively.

A Fast Configuration Method for Load Balancing in Distribution System (배전계통의 부하평형을 고려한 고속 계통구성방법)

  • Nam, Kee-Young;Choi, Sang-Bong;Jeong, Seong-Hwan;Ryoo, Hee-Suk;Kim, Ho-Yong
    • Proceedings of the KIEE Conference
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    • 2003.07a
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    • pp.381-383
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    • 2003
  • The authors have developed a solution method based on the incremental algorithm. The optimal load balance between feeders is found through the incremental algorithm with allowing the loop configuration. Therefore loops can be configured in the resulting configuration, and then we must eliminate all loops to satisfy radiality of distribution system because the distribution system is operated under radial status. But the optimal load balance through incremental algorithm is deteriorated when the loops are eliminated. In this paper, authors propose a new method which is to improve the searching procedure of path in the incremental algorithm, that is, so as not to make loop in the path configuration.

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A Study on the Characteristics of Boiling Heat Transfer of Two-Phase Loop Thermosyphons (루우프형 2상 유동 열사이폰의 비등열전달 특성에 관한 연구)

  • Cho, Dong-Hyun
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.3
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    • pp.639-646
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    • 2014
  • A heat exchanger using two-phase loop thermosyphons was developed as a waste heat recovery system. An experimental study was carried out on the heat transfer characteristics of two-phase loop thermosyphons heat exchanger and the results from the experiments were used to see the possibility which the two-phase loop thermosyphons could be an alternate solution for waste heat recovery system. In the present work, R134a has been used as the working fluid and the filling rate do working fluid and heat flux have been used as the experimental parameters. The results show that the filling rate of working fluid and heat flux are very important factors for the operation of two-phase loop thermosyphons. The experimental results showed the provisional results as a waste heat recovery system.

A Convenient Radiolabeling of [$^{11}$C](R)-PK11195 Using Loop Method in Automatic Synthesis Module ($^{11}$C 표지 자동합성장치에서 루프법을 이용한 ($^{11}$C)(R)-PK11195의 간편한 합성법)

  • Lee, Hak-Jeong;Jeong, Jae-Min;Lee, Yun-Sang;Kim, Hyung-Woo;Choi, Jae-Yeon;Lee, Dong-Soo;Chung, June-Key;Lee, Myung-Chul
    • Nuclear Medicine and Molecular Imaging
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    • v.43 no.4
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    • pp.337-343
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    • 2009
  • Purpose: ((R)-1-(2-chlorophenyl)-N-1-[$^{11}$C]methyl-N(1-propyl)-3-isoquinoline carboxamide ((R)-PK11195) is a specific ligand for the peripheral type benzodiazepine receptor and a marker of activated microglia, used to measure inflammation in neurologic disorders. We report here that a direct and simple radiosynthesis of [$^{11}$C](R)-PK11195 in mild condition using NaH suspension in DMF and one-step loop method. Materials and Methods: (R)-N-Desmethyl-PK11195 (1 mg) in DMSO (0.1 mL) and NaH suspension in DMF (0.1 mL) were injected into a semi-prep HPLC loop. [$^{11}$C]methyl iodide was passed through HPLC loop at room temperature. Purification was performed using semi-preparative HPLC. Aliquots eluted at 11.3 min were collected and analyzed by analytical HPLC and mass spectrometer. Results: The labeling efficiency of [$^{11}$C](R)-PK11195 was 71.8$\pm$8.5%. The specific activity was 11.8:$\pm$6.4 GBq/$\mu$mol and radiochemical purity was higher than 99.2%. The mass spectrum of the product eluted at 11.3 min showed m/z peaks at 353.1 (M+1), indicating the mass and structure of (R)-PK11195. Conclusion: By the one-step loop method with the [$^{11}$C]CH3l automated synthesis module, [$^{11}C$](R)-PK11195 could be easily prepared in high radiochemical yield using NaH suspension in DMF.

The Analysis of System Thinking on the Commercialization of Public R&D Research Institute (공공 R&D 기관의 기술 상용화 과정에 관한 시스템 사고 분석)

  • Kim, Soon-Sun;Kim, Dong-Hwan
    • Korean System Dynamics Review
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    • v.8 no.2
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    • pp.191-207
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    • 2007
  • Nowadays almost nations try to promote the competition of nation through the development of national science technology. Also Korea has been engaging in this race of through public research&development institute. But recently the commercialization of R&D products appears a poor result in the royalty of technology according weak technology transfer toward IT small and medium-sized enterprises. Even If we have been trying to find the problems and causes, as yet it is true that we don't solve clearly the problem, make a diagnosis about it. This paper analyzes the process of commercialization in public R&D research institute(ETRI: Electronics and Telecommunications Research Institute) through system thinking and researches an interrelationship among government agency, public R&D research institute, and enterprise. This one can find the fact that between research fund and R&D products, transfer technology and royalty, and enterprise's operating profit and active technology transfer like adding technology development is a positive feedback loop. This positive feedback loop in the commercialization of public R&D research institute carries out very important role in examining problems in R&D product's commercialization. Also this paper looks forward to being a guide in the commercialization of public R&D research institute.

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Genetic Variation of Mitochondrial DNA in Duroc (Sus Scrofa) Using Single Stranded Conformation Polymorphism Analysis (Single Stranded Conformation Polymorphism 분석에 의한 돼지 Duroc 품종의 미토콘드리아 DNA 유전적 변이)

  • Cho, I.C.;Jung, Y.H.;Jung, J.K.;Seong, P.N.;Kim, B.W.;Lee, J.G.;Jeon, J.T.
    • Journal of Animal Science and Technology
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    • v.45 no.6
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    • pp.911-916
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    • 2003
  • The mitochondrial DNA(mtDNA) D-loop region was amplified from Duroc(Sus scrofa) by polymerase chain reaction(PCR). The oligonucleotide primer used to amplify the Sus scrofa mtDNA D-loop region was designed using tRNA-Pro and tRNA-Phe sequence in mtDNA regions highly conserved in many other animal species. There were 1,145 base pairs(bp) in the D-loop region. The middle of the region contained 10 tandem repeat of an 10-bp Sus scrofa-specific sequence, TACACGTGCG. We designed primers for PCR-mediated single stranded conformation polymorphism(SSCP) analysis that amplified a 345 bp fragment, which contained the most variable region according to our sequencing data. SSCP analysis of denatured amplification products was carried out by polyacrylamide(8%) gel electrophoresis followed by ethidium bromide staining. The SSCP analysis identified two band patterns(A and B) and comparision of these two nucleotide sequences identified 21 base substitutions. These results show that SSCP analysis of the D-loop region is useful for detecting the genetic polymorphism.

Analysis of Higher Order Structure of 5S rRNA from Pseudomonas Alcaligenes by using($\eta^{6}$-mesitylene) manganese-(Ⅰ) Tricarbonyl hexafluorophosphate (($\eta^{6}$-Mesitylene) manganese-(Ⅰ) Tricarbonyl hexafluorophosphate를 사용한 Pseudomonas Alcaligenes 5S rRNA의 고차원 구조 분석)

  • Kim, Sang Bumn;Park, In Won
    • Journal of the Korean Chemical Society
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    • v.42 no.2
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    • pp.209-213
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    • 1998
  • The higher order structure of Pseudomonas alcaligenes 5S rRNA has been investigated by using ($\eta^{6}$-mesitylene) manganese (Ⅰ) tricarbonyl hexafluorophosphate[MTH-Mn (Ⅰ)], dimethylpyrocarbonate, potassium permanganate as chemical probes. The sequences cleaved strongly by MTH-Mn (Ⅰ) on the tertiary structure of the 5S rRNA are $G_{12}AUGG_{16}$ of loop a, $G_{51}AAGUGAAGC_{60}$ of the region b-C, $U_{65}-AGCG_{69}$. of the region B-a, and $G_{72}AUGG_{76}$ of loop d. Based on such cleavage patterns of 5S rRNA by MTH-Mn(Ⅰ) and other chemical probes, we presume that the sequences strongly cleaved form pocket-like structure as in the the corner of L structure of $tRNA^{Phe}$. We also presume that the region b-C and loop d together play a role of hinge in forming the pocket-like structure in the 5S rRNA.

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