• Journal of Microbiology and Biotechnology
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• v.3 no.4
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• pp.217-223
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• 1993

To investigate the nature and location of the nisin-resistance determinant of Lactococcus lactis subsp. lactis 7962 (L. lactis 7962), a total plasmid DNA prepared from L. lactis 7962, a nisin producer, was used to transform L. lactis subsp. lactis LM0230, a plasmid-free and nisin-sensitive strain, by protoplast mediated transformation procedures. All of the nisin-resistant transformants acquired the ability to utilize sucrose at the same time, confirming the close linkage between these two determinants in L. lactis 7962. The plasmid DNA profiles of a few selected nisin-resistant transformants were examined by agarose gel electrophoresis. No common plasmid was found among the transformants and some small plasmids previously not present in L. lactis 7962 were detected. These transformants were named as L. lactis KL1, KL2, KL3, KL4, or KL5, respectively based on their plasmid profiles. Growth curves of all transformants were similar to that of L. lactis LM0230, but different from that of L. lactis 7962. L. lactis KL5 showed the highest level of resistance to nisin, growing up to 1, 200 IU nisin/ml after 40 hr incubation. Some nisin-sensitive derivatives of KL1 or KL2 were obtained by plasmid curing experiments. The plasmid DNA profiles of the nisin-sensitive KL1 derivatives were apparently the same as that of the KL1. All of the nisin-sensitive KL2 derivatives were plasmid-free, but a nisin-resistant strain with no apparent plasmid was also obtained. These results indicate that the nisin-resistance of the $Nis^r$ transformants is presumably mediated by the chromosomally located gene(s) rather than plasmid-encoded gene(s).

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.201-210
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• 2007

Soybean [Glycine max(L.) Merr.] oil is versatile and used in many products. Modifying the fatty acid profile would make soy oil more functional in food and other products. The ideal oil with the most end uses would have saturates(palmitic + stearic acids) reduced from 15 to < 7%, oleic acid increased from 23 to > 55%, and linolenic acid reduced from 8 to < 3%. Reduced palmitic acid(16:0) is conditioned by three or more recessive alleles at the Fap locus. QTLs for reduced palmitic acid have mapped to linkage groups(LGs) A1, A2, B2, H, J, and L. Genes at the Fad locus control oleic acid content(18:1). Six QTLs($R^2$=4-25%) for increased 18:1 in N00-3350(50 to 60% 18:1) explained four to 25% of the phenotypic variation. M23, a Japanese mutant line with 40 to 50% 18:1 is controlled by a single recessive gene, ol. A candidate gene for FAD2-1A can be used in marker-assisted breeding for high 18:1 from M23. Low linolenic acid(18:3) is desirable in soy oil to reduce hydrogenation and trans-fat accumulation. Three independent recessive genes affecting omega-3 fatty acid desaturase enzyme activity are responsible for the lower 18:3 content in soybeans. Linolenic acid can be reduced from 8 to about 4, 2, and 1% from copies of one, two, or three genes, respectively. Using a candidate gene approach perfect markers for three microsomal omega-3 desaturase genes have been characterized and can readily be used in for marker assisted selection in breeding for low 18:3.

• Journal of Ginseng Research
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• v.20 no.4
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• pp.389-415
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• 1996

Panax ginseng C.A. Meyer(Araliaceae) has been traditionally used as an expensive and precious medicine in oriental countries for more than 5, 000 years. Ginseng saponin isolated from the root of Panax ginseng have been regarded as the main effective components responsible for the pharmacological and biological activities. Such as antiaging effects. antidiabetic effects anticancer effects. Protection against physical and chemical stress. Analgesic and antipyretic effects. Effects on the central nervous system, tranquilizing action and others. Thirty kinds of ginsenosides have been so far isolated from ginseng saponin and their chemical structures have been elucidated since 1960's. Among which protopanaxadiol type is 19 kinds. protopanaxatriol type. 10 kinds and oleanane type, one. Since ginsenosides are generally labile under acidic conditions ordinary acid hydrolysis is always accompanied by many side reactions, such as epimerization. hydroxylation and cyclization of side chain of the sapogenins Especially. it is well known that C-20 glycosyl linkage of ginsenoside was hydrolysed on heating with acetic acid to give an equilibrated mixture of 20(S) and 20(R) epimers. And also, the chemical transformations of the secondary metabolites have appeared during the steaming process to prepare red ginseng. Indicating demalonylation of malonyl ginsenosides, elimination of glycosyl residue at C-20 and isomerization of hydroxyl configuration at C-20. But these studies have not provided a comprehensive picture in explaning how these ginsenosides showed val'iotas pharmacological activities of ginseng. Though some of them have been involved in the mechanism of pharmacological actions. Recently, non-saponin components have received a great deal of attention for their antioxidant, anticancer antidiabetic, immunomodulating. anticomplementary activities and so on. To meet the demand for such wide applications, studies on the non-saponin components play an important role in providing a good evidence of pharmacological and biol ogical activities. Among the non-saponin constituents of Korean ginseng, polyacetylenes, phenols. Sesquiterpenes, alkaloids. polysaccharides oligosaccharides, oligopeptides and aminoglycosides together with ginsenosides of terrestrial part are mainly described.

• Journal of the Economic Geographical Society of Korea
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• v.1 no.1
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• pp.81-112
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• 1998

This study reviews the industrialization process of the United States-Mexico Borderlands and the economic relations between the U.S. and Mexico and examines their impact on the borderlands. Main factor in the industrialization of the borderlands was the U.S. investment on the maquiladora program of Mexico since 1965. It contributed to the increase in employment and population of borderlands and the development of service industries. Low wage level of Mexico induced not only standardized labor-intensive industries but also the high-tech automated industries because they still use a lot of labor in manufacturing and assembly process, while the functions of management. R & D, and distribution remained in the U.S. This is a typical case of international division of labor and satellite industrial district. The rules of origin in NAFTA, however forced branch Plants of multinational companies to form the local linkages between firms.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.30 no.5
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• pp.945-956
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• 2020

The publication of data is essential in order to receive high quality services from many applications. However, if the original data is published as it is, there is a risk that sensitive information (political tendency, disease, ets.) may reveal. Therefore, many research have been proposed, not the original data but the synthetic data generating and publishing to privacy preserve. but, there is a risk of privacy leakage still even if simply generate and publish the synthetic data by various attacks (linkage attack, inference attack, etc.). In this paper, we propose a synthetic data generation algorithm in which privacy preserved by applying differential privacy the latest privacy protection technique to GAN, which is drawing attention as a synthetic data generative model in order to prevent the leakage of such sensitive information. The generative model used CGAN for efficient learning of labeled data, and applied Rényi differential privacy, which is relaxation of differential privacy, considering the utility aspects of the data. And validation of the utility of the generated data is conducted and compared through various classifiers.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.3
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• pp.341-349
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• 2019

Objective: Internal organs indirectly affect economic performance and well-being of animals. Study of internal organs during later layer period will allow full utilization of layer hens. Hence, we conducted a genome-wide association study (GWAS) to identify potential quantitative trait loci or genes that potentially contribute to internal organ weight. Methods: A total of 1,512 chickens originating from White Leghorn and Dongxiang Blue-Shelled chickens were genotyped using high-density Affymetrix 600 K single nucleotide polymorphism (SNP) array. We conducted a GWAS, linkage disequilibrium analysis, and heritability estimated based on SNP information by using GEMMA, Haploview and GCTA software. Results: Our results displayed that internal organ weights show moderate to high (0.283 to 0.640) heritability. Variance partitioned across chromosomes and chromosome lengths had a linear relationship for liver weight and gizzard weight ($R^2=0.493$, 0.753). A total of 23 highly significant SNPs that associated with all internal organ weights were mainly located on Gallus gallus autosome (GGA) 1 and GGA4. Six SNPs on GGA2 affected heart weight. After the final analysis, five top SNPs were in or near genes 5-Hydroxytryptamine receptor 2A, general transcription factor IIF polypeptide 2, WD repeat and FYVE domain containing 2, non-SMC condensin I complex subunit G, and sonic hedgehog, which were considered as candidate genes having a pervasive role in internal organ weights. Conclusion: Our findings provide an understanding of the underlying genetic architecture of internal organs and are beneficial in the selection of chickens.

• Journal of Convergence for Information Technology
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• v.12 no.1
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• pp.163-170
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• 2022

With the advent of the 4th Industrial Revolution, solutions that apply AI technology are being actively developed. Since 2017, the introduction of business automation solutions using AI-based Robotic Process Automation (RPA) has begun in the financial sector and insurance companies, and recently, it is entering a time when it spreads past the stage of introducing RPA solutions. Among the business automation using these RPA solutions, it is very important how accurately textual information in the document is recognized for business automation using various documents. Such character recognition has recently increased its accuracy by introducing deep learning technology, but there is still no recognition model with perfect recognition accuracy. Therefore, in this paper, we checked how much accuracy is improved when pre- and post-processor technologies are applied to deep learning-based character recognition engines, and implemented RPA recognition engines and linkage technologies.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.26 no.6
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• pp.881-889
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• 2022

As interest in environmental pollution caused by ship emissions is increasing worldwide, many studies are being conducted on the development of systems that increase energy efficiency to reduce ship emissions. In order to test the energy management system controller driven in real time on a large ship, it is necessary to perform the test through the simulator. In this study, addresses were set for each signal according to Modbus TCP/IP so that each control system and energy management system could be linked, and the algorithm was configured according to the signal flow. In addition, the signal generator was designed and manufactured so that each controller could artificially generate the signal collected from the vessel. As a result of the simulator production and interlocking, it was confirmed that each controller operated in real time performed its role appropriately, and that the algorithm of the ship energy management system was properly applied.

• Genomics & Informatics
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• v.19 no.4
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• pp.37.1-37.7
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• 2021

Genome-wide association studies (GWASs) facilitated the discovery of countless disease-associated variants. However, GWASs have mostly been conducted in European ancestry samples. Recent studies have reported that these European-based association results may reduce disease prediction accuracy when applied in non-Europeans. Therefore, previously reported variants should be validated in non-European populations to establish reliable scientific evidence for precision medicine. In this study, we validated known associations with type 2 diabetes (T2D) and related metabolic traits in 125,850 samples from a Korean population genotyped by the Korea Biobank Array (KBA). At the end of December 2020, there were 8,823 variants associated with glycemic traits, lipids, liver enzymes, and T2D in the GWAS catalog. Considering the availability of imputed datasets in the KBA genome data, publicly available East Asian T2D summary statistics, and the linkage disequilibrium among the variants (r² < 0.2), 2,900 independent variants were selected for further analysis. Among these, 1,837 variants (63.3%) were statistically significant (p ≤ 0.05). Most of the non-replicated variants (n = 1,063) showed insufficient statistical power and decreased minor allele frequencies compared with the replicated variants. Moreover, most of known variants showed <10% genetic heritability. These results could provide valuable scientific evidence for future study designs, the current power of GWASs, and future applications in precision medicine in the Korean population.

• Journal of Advanced Navigation Technology
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• v.27 no.2
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• pp.167-172
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• 2023

The P-3C maritime patrol aircraft operated by the Republic of Korea Navy is equipped with various sensor devices (LRUs, line replace units) for tactical data collection. Depending on the characteristics of the sensor device, it operates with various communication protocols such as IEEE 802.3, MIL-STD-1553A/B, and ARINC-429. In addition, the collected tactical data is processed in the tactical station for mission operators, and this tactical station constitutes a clustering network on Gigabit Ethernet and operates in a distributed processing method. For communication with the sensor device, a specific tactical station mounts a peripheral device (eg. ARINC-429 interface card). The problem is that the performance of the entire distributed processing according to the peripheral device control and communication relay of this specific device is degraded, and even the operation stop of the tactical station has a problem of disconnecting the communication with the related sensor device. In this paper, we propose a method to mount a separate gateway to solve this problem, and the validity of the proposed application is demonstrated through the operation result of this gateway.