• Journal of Applied Biological Chemistry
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• 제57권1호
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• pp.33-40
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• 2014

Polysaccharides have attracted great attention for their wide range of applications in biological and medical fields. In this paper, the interaction of polysaccharides with human serum albumin (HSA) was systematically investigated by fluorescence (FL) spectroscopy and circular dichroism (CD) spectra under different conditions. The Stern-Volmer quenching constants ($K_a$) at different ionic strength and pH were calculated, and information of the structural features of HSA was discussed. FL and CD results indicate that both hydrophobic and electrostatic interactions play important roles during the binding process. The quenching of the fluorescence resulting the binding of polysaccharides and HSA is static.

• 항공우주시스템공학회지
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• 제2권3호
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• pp.19-23
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• 2008

We have fabricated pressure sensitive paint films consisting of PtTFPP activators dispersed in fluorinated polymer matrix. This matrix material is unique in its composition and structure. We evaluated the oxygen quenching effects, and thus the pressure sensitivity, of the fabricated films in terms of various processing parameters. Based on the experimental results, we anticipate that a good but new pressure sensitive paint would be presented through further elaboration of the polymer compositions and optimization of the processing conditions.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 1997년도 춘계학술대회논문집
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• pp.240-243
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• 1997

The Quenching and Self Tempering (QST) rolling is treated in terms of an advance process on Controlled Rolling and Cooling Technology (CRCT). In the analysis, the effect of this process is governed by both quenching and finishing conditions in the related with temperature. The objective of the QST model is to simulate the temperature gradient of the stock being rolled in the rolling mill. A comparison of computer simulated and manufactured micro structure as well as mechanical properties shows a good consistency. The micro structure of this high-strength round bar consists of tempered martensite and ferrite + pearlite phases.

• 열처리공학회지
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• 제2권2호
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• pp.11-16
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• 1989

Understanding and control of thermal history of heat-treated components are very important in heat treating process. A computer program was developed for prediction of thermal history in quenching process with various cooling media and agitation conditions. Computer simulation of heat flow in quenching treatment of spur gear of SCM 22 H was carried out by two and three dimensional finite difference method. Distributions of microstructure and hardness in heat-treated spur gear were predicted by computer simulation, and the results showed a good agreement with the experiments. It was concluded that the

• 한국표면공학회지
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• 제57권3호
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• pp.155-164
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• 2024

Physical properties of carbon nanomaterials are dependent on their nanostructures and they are modified by diverse synthesis methods. Among them, thermal plasma method stands out for synthesizing carbon nanomaterials by controlling chemical and physical reactions through various design and operating conditions such as plasma torch type, plasma gas composition, power capacity, raw material injection rate, quenching rate, kinds of precursors, and so on. The method enables the production of carbon nanomaterials with various nanostructures and characteristics. The high-energy integration at high-temperature region thermal plasma to the precursor is possible to completely vaporize precursors, and the vaporized materials are rapidly condensed to the nanomaterials due to the rapid quenching rate by sharp temperature gradient. The synthesized nanomaterials are averagely in several nanometers to 100 nm scale. Especially, the thermal plasma was validated to synthesize low-dimensional carbon nanomaterials, carbon nanotubes and graphene, which hold immense promise for future applications.

• 한국산학기술학회논문지
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• 제18권5호
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• pp.252-261
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• 2017

본 연구에서는 유정용 강관의 열처리 조건에 따른 미세조직 및 기계적 성질을 조사하였다. 실험에 사용된 유정용 강관의 종류는 J55 강재를 사용 하였고, 열처리 조건은 각각 오스테나이트 처리온도 ($880^{\circ}C$, $910^{\circ}C$, $940^{\circ}C$), 냉각방식 (수냉, 유냉), 템퍼링 온도 (미실시, $550^{\circ}C$, $650^{\circ}C$) 이다. 열처리 조건에 따라 얻어지는 미세조직을 예측하기 위해 J55 강재의 화학적 성분을 기준으로 평형상태도와 CCT 곡선을 시뮬레이션 하였으며, 그 결과 A1, A3 온도가 약 $20^{\circ}C$ 감소 하는 결과를 얻을 수 있었다. 냉각속도에 따라 마르텐사이트, 베이나이트, 페라이트등 예상되는 미세조직을 정성적으로 알 수 있었고 이 결과는 실제 실험값과 유사한 양상을 나타내었다. 오스테나이트 처리온도가 증가 함에 따라 구 오스테나이트 결정립이 조대화 되었으며, 특히 $920^{\circ}C$ 이상에서 결정립 크기가 급격히 증가하였다. 따라서 결정립 미세화 효과에 따라 경도와 강도는 감소 하였다. 열처리 조건이 변화함에 따라 마르텐사이트, 베이나이트, 페라이트 등 다양한 조직이 생성되었으며, 이는 경도, 강도 및 연신율에 큰 영향을 미쳤다. 수냉의 경우 마르텐사이트 조직이, 유냉의 경우 베이나이트와 페라이트 조직이 형성되었으며 수냉한 시편이 더 우수한 기계적 성질을 나타내었다. 템퍼링 처리후 마르텐사이트 조직 내에 FeC 석출물이 생성되었고 템퍼링 처리온도가 증가함에 따라 FeC석출물이 조대화되면서 인성이 향상되었다.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제3권1호
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• pp.11-21
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• 1999

The effect of sulfite on barley seedlings was investigated through Chl content, the electron transport activity of the photosystem, and Chl fluorescence. Barley leaves were harvested every 12 hrs during greening periods, and were then treated with a sulfite solution in either light or dark conditions. In both cases, the Chl content decreased in comparison with the control at any greening period. After sulfite treatment in the light, the activity of PS I decreased slightly, yet that of PSII showed a decrease of about 15%. The values of Fv, qP and qE decreased, however, the value of ql increased compared with the control. In addition, the value of qE decreased in leaves greened more than 12 hrs compared with that of the control. This indicates that the photosynthetic complex involved in energy dependent fluorescence quenching is undeveloped in a 12 hrs greened leaf, accordingly, it was a hardly affected by sulfite. After sulfite treatment in the dark, the activities of PSII and PSI decreased slightly, there was a small change in the value of Fv, qP decreased, and qE and the ratio of qNP/q increased in comparison with the control. As a result, PSII and PSI were not inhibited, however, the redox of QA was inhibited, and the excited energy was lost through the nonphotochemical pathway. The effects of sulfite in light or dark conditions were not considerably different with the Chl fluorescence quenching analysis method. In both light and dark conditions, the value of qP significantly decreased with sulfite compared to that of the control. This implies that the redox of QA was inhibited by sulfite in both light and dark contions.

• 열처리공학회지
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• 제28권6호
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• pp.300-309
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• 2015

The aim of the present study was to predict the variations in microstructure and deformation occurring during gas carburizing and quenching processes of a SCM420H planetary gear in a real production environment using the finite element method (FEM). The motivation for the present study came from the fact that previous FEM simulations have a limitation of the application to the real heat treatment process because they were performed with material properties provided by commercial programs and heat transfer coefficients (HTC) measured from laboratory conditions. Therefore, for the present simulation, many experimentally measured material properties were employed; phase transformation kinetics, thermal expansion coefficients, heat capacity, heat conductivity and HTC. Particularly, the HTCs were obtained by converting the cooling curves measured with a STS304 gear without phase transformations using an oil bath with an agitator in a real heat treatment factory. The FEM simulation was successfully conducted using the aforementioned material properties and HTC, and then the predicted results were well verified with experimental data, such as the cooling rate, microstructure, hardness profile and distortion.

• 대한기계학회논문집A
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• 제36권10호
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• pp.1221-1226
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• 2012

핫 스탬핑은 오스테나이트 변태 온도 이상에서 프레스 성형 후 급속히 냉각되는 고강도 부품을 제조하는 성형 방법이다. 매우 적은 양의 보론 성분을 가지고 있는 보론강은 핫 스탬핑에 사용되는 재료 중의 하나이다. 본 연구의 목적은 열처리 조건에 따르는 기계적 성질과 에릭슨 커핑 시험에 의하여 성형성을 조사하는 것이다. 다양한 온도에서 다이��칭은 대기 시간을 달리하여 실시하였다. 1173 K-0s에서 ��칭 후 TWB는 1203 MPa의 인장 강도를 얻었다. 이것은 모재 인장강도(1,522 MPA)의 79 %이다. 금형 온도(298, 523, 673 K)에 따른 보론강 TWB의 성형성은 차이가 크지 않았다. 그러나 성형 속도가 증가함에 따라 성형성이 감소하는 것을 확인할 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제31권6호
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• pp.1479-1484
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• 2010

Photosynthesis uses light energy to drive the oxidation of water at an oxygen-evolving catalytic site within photosystem II (PSII). Chlorophyll binding by the photosystem II subunit S protein, PsbS, was found to be necessary for energy-dependent quenching (qE), the major energy-dependent component of non-photochemical quenching (NPQ) in Arabidopsis thaliana. It is proposed that PsbS acts as a trigger of the conformational change that leads to the establishment of nonphotochemical quenching. However, the exact structure and function of PsbS in PSII are still unknown. Here, we clone and express the recombinant PsbS gene from Arabidopsis thaliana in E. coli and purify the resulting homogeneous protein. We used various biochemical and biophysical techniques to elucidate PsbS structure and function, including circular dichroism (CD), fluorescence, and DSC. The protein shows optimal stability at $4^{\circ}C$ and pH 7.5. The CD spectra of PsbS show that the conformational changes of the protein were strongly dependent on pH conditions. The CD curve for PsbS at pH 10.5 curve had the deepest negative peak and the peak of PsbS at pH 4.5 was the least negative. The fluorescence emission spectrum of the purified PsbS protein was also measured, and the ${\lambda}_{max}$ was found to be at 328 nm. PsbS revealed some structural changes under varying temperature and oxygen gas condition.