• Archives of Pharmacal Research
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• 제17권2호
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• pp.76-79
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• 1994

The concentration of styrne in blood of the occupationally syrene-exposed workers was checked by gas chromatographic headspace analysis. Mandelic acd in urine, that is a major metabolite of styrene, and hippuric acid wre also analyzed by high performance liquid chromatography. For the biological monitoring of styrene-exposed workers, the routine method of the quantitative determination of styrene nad its metabolites in the biolgical samples were studied.

• 생약학회지
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• 제30권1호
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• pp.48-53
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• 1999

Isolation and quantitative determination of costunolide from Saussurea lappa Clarke (Compositae) has been conducted by using HPLC method. Costunolide in an acetone extract from the crude drug was separated on a RP-18 column using a $MeOH-H_20$ mixture (65:35) as an eluent and the average content is about $1.32{\sim1.42%.$ The content of costunolide in dried extract was decreased by about 24% in seven days. However it showed a slight decrease in solution. It is highly recommended that quantitative determination of costunolide from Saussureae Radix should be conducted as early as possible after solvent extraction.

• 생약학회지
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• 제29권1호
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• pp.18-21
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• 1998

A method for isolation and quantitative determination of patchouli alcohol from Pogostemonis Herba (Pogostemon cablin) has been developed. Isolation of patchouli alcohol was achieved by column chromatography employing the normal-phase gradient separation system n-hexane-ether on silica gel column. The GC method for quantitative determination of patchouli alcohol provided a method for standardization of the raw drug. It suggested that the average content of patchouli alcohol in Pogostemonis Herba is about 0.26%.

• 생약학회지
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• 제30권2호
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• pp.163-167
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• 1999

A method for isolation and qantitative determination of anemarsaponin B from the rhizomes of Anemarrhena asphodeloides has been developed. Isolation of anemarsaponin B was achieved by silica gel and RP-18 column chromatography. The HPLC method for quantitative determination of anemarsaponin B provided a method for standardization of the crude drug. It suggested that the content of anemarsaponin B in Anemarrhena asphodeloides is about 0.12-1.48%.

• 생약학회지
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• 제32권2호통권125호
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• pp.153-156
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• 2001

The methods for isolation and quantitative determination of dioscin, a spirostanol triglycoside, from the roots of Smilax china have been developed. Isolation of dioscin was achieved by silica gel and RP-18 chromatography. The HPLC method used for quantitative determination of dioscin enabled the standardization of the crude drug. It suggests that the content of dioscin in Smilax china should be above 0.01%.

• Journal of Pharmaceutical Investigation
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• 제15권3호
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• pp.151-159
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• 1985

For the determination of aliphatic carbamates, the quantitative analysis of carisoprodol was investigated by gab chromatography (GC) and spectrophotometry. All the methods studied were found to be very quantitative. The minimum experimental amounts of GC method, spectrophotometric method I and II were approximately $10^{-9},\;10^{-5}$ and $10^{-8}$ mole, respectively. The obtained results showed that GC method I was much more sensitive and rapid than spectrophotometric method II.

• Applied Biological Chemistry
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• 제17권1호
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• pp.49-53
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• 1974

Mohan's aniline procedure for the quantitative determination of niacin in feed additives containing various vitamines and minerals was compared with microbiological procedure utilizing growth rate of Lactobacillus arabinosus 17-5. Microbioassay was more sensitive than chemical procedure to the detection of standard niacin and was applicable to the determination of minute amounts of niacin. while both microbioassay and chemical proce dure were discovered to be unsatisfactory by the recovery test for the determination of niacin in feed additives containing much interfering substances. But the possibility of the determination of niacin in feed additives on chemical prccedure together with microbioassay was proved.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.772-776
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• 2004

The paper describes the quantitative determination of out-of-plane displacement from result of Shearogrpahy, which can measure the first-order partial derivative of out-of-plane displacement directly. However, the differential sensitivity of Shearography is related to the amount of shearing, which is manually adjustable in optical interferometer and affects the quantitative determination. The relationship between those is inspected by comparing ESPI with Shearography. From the result, the amount of shearing plays a modulation factor of out-of-plane displacement and small amount of shearing gives good agreement with out-of-plane displacement.

• Natural Product Sciences
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• 제19권2호
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• pp.145-149
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• 2013

A simple reversed phase HPLC method was established for quantitative determination of liquiritigenin (1), genistein (2), isoliquiritigenin (3), and 7-hydroxyflavanone (4) from stems of Spatholobus suberectus Dunn (Leguminosae) using a binary gradient of $H_2O$ and MeOH as a mobile phase with UV detection at 280 nm. All calibration curves showed good linear regression ($r^2$ > 0.998) within test ranges. The detection limits of the four compounds were $0.43{\sim}1.63{\mu}g/mL$. The contents of four flavonoids (1 - 4) from the stem of S. suberectus were 6.54 mg/g, 1.66 mg/g, 6.65 mg/g, and 1.93 mg/g, respectively.

• 생약학회지
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• 제30권3호
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• pp.332-334
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• 1999

Corydalis Tuber has been used in traditional medicine for an analgesic, antispasmodic and gastric ulcers. For the quality control on this drug, isolation and quantitative determination of berberine and coptisine from Corydalis ternata Nakai (Papaveraceae) has been conducted by using HPLC method. Berberine and coptisine in quarternary alkaloidal fraction from the crude drug were separated on silicagel column using a $CHCl_3:MeOH\;(85:15)$ and $CHCl_3:MeOH:H_2O\;(70:30:4)$ as an eluent, and the average contents were about 0.93 and 0.36%.