• Mass Spectrometry Letters
• /
• v.13 no.4
• /
• pp.139-145
• /
• 2022

Protein glycosylation is a common post-translational modification by non-template-based biosynthesis. In fungal biotechnology, which has great applications in pharmaceuticals and industries, the importance of research on fungal glycoproteins and glycans is accelerating. In particular, the importance of quantitative analysis of fungal glycans is emerging in research on the production of filamentous fungal proteins by genetic modification. Reliable mass spectrometry-based techniques for quantitative glycomics have evolved into chemical, enzymatic, and metabolic stable isotope labeling methods. In this study, we intend to expand quantitative glycomics by metabolic isotope labeling of glycans in Aspergillus niger, a filamentous fungus model, by the MILPIG method. We demonstrate that incubation of filamentous fungi in a culture medium with carbon-13 labeled glucose (1-¹³C₁) efficiently incorporates carbon-13 into N-linked glycans. In addition, for quantitative validation of this method, light and heavy glycans are mixed 1:1 to show the performance of quantitative analysis of various N-linked glycans simultaneously. We have successfully quantified fungal glycans by MILPIG and expect it to be widely applicable to glycan expression levels under various biological conditions in fungi.

• Journal of the Korean Society of Systems Engineering
• /
• v.20 no.spc1
• /
• pp.1-11
• /
• 2024

Today, the importance of test and evaluation of defense systems is increasing day by day. In performing efficient defense systems test works, process improvement based on quantitative management is essential. The purpose of this paper is to present the results of process improvement for the defense systems test works of the test organization based on quantitative management activities. As a methodology to confirm process improvement performance, the 'MPM(Managing Performance and Measurement)' practice area of the CMMI model was applied. The quantitative management model for defense systems test works was developed so that it could be practically applied not only to the entire test organization but also to the organization at the department level that actually performs the test work. Finally, the application cases of the quantitative management model for defense system test works and the results of process improvement were described.

• Proceedings of the KIEE Conference
• /
• 2008.04a
• /
• pp.129-130
• /
• 2008

In this study, we implement the color image processing algorithm to analyze the quantitative assessment of denticulus by computing spectral characteristics in HGB color domain. We can observe that we can utilize the normalized HGB color feature to assess the quantitative formation of denticulus.

• Environmental Analysis Health and Toxicology
• /
• v.4 no.1_2
• /
• pp.11-17
• /
• 1989

A quantitative analytical method of highly concentrated PCB is established in the research. With the quantitative analytical method PCB of insulating oil was examined. The following conclusions are derived from this research. 1. The recovery ratio was 95.7% with the quantitative analytical method, which indicates it can be used for the analysis of PCB. 2. PCB concentration of insulation oil in waste condenser was found to be 21.2% thru 13.85% , which was highly concentrated. 3. PCB insulation oil in waste condenser was same as PCB-42.

• 한국방재학회:학술대회논문집
• /
• 2008.02a
• /
• pp.645-648
• /
• 2008

This study provides considerations for quantitative risk assessment of landslide on GIS technology. It shows how the landslide possibility analysis is linked by GIS modeling to provide loss estimation tools for landslide hazards in support of socio-economic loss reduction efforts. Those risk assessment results can deliver factual damage situation prediction to policy making for the landslide damage mitigation.

• KIPS Transactions on Software and Data Engineering
• /
• v.1 no.2
• /
• pp.75-80
• /
• 2012

There are many problems that cause the process improvement of software and hardware, personality errors during software development. This paper propose the quantitative analysis of error that removes and manages the system problems as well.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.19 no.39
• /
• pp.219-227
• /
• 1996

For the equitable and objective evaluation of the performances of R & D (Research and Development) Projects, the quantitative evaluation of research products was performed. In this research, the AHP(Analytic Hierarchy Process) technique was used to calculate the relative importance(weight) of research products such as patents, thesis, conferences and man-power with make up individual questions. With calculated relative weight of research products, the model that gives the quantitative grades of research products was studied.

• Architectural research
• /
• v.12 no.2
• /
• pp.41-52
• /
• 2010

The aim of this paper is to evaluate the methodological strategies for the architectural design field of study mainly focused on qualitative and quantitative research designs. Firstly, this paper addresses the characteristics of the six approaches including their methodological aspects in general. Each strategy is assessed by the different approaches to give full insights in it. Secondly, it distinguishes the differences among six research approaches especially derived from qualitative and quantitative research designs. The differences are discussed in terms of the strengths and weaknesses of each strategy. Finally, this paper attempts to discuss about possible applications for introducing approaches to the research topic with which the exemplified research topic, a design evaluation system, deals. To investigate the applicability of the design methods employed, the following topic has been stated; how to develop a design evaluation system and what to be considered for unfolding the thrown topic in terms of strategic approaches in the field of architectural design researches reviewed through the study.

• Journal of Environmental Science International
• /
• v.3 no.3
• /
• pp.285-296
• /
• 1994

The objective of this study was to examine and compare to transient response to quantitative and hydraulic shocks which produce equal changes in mass rate of organic feed in aerobic fixed-film process. The general experimental approach was to operate the system at several growth rates under steady-state(pre-shock) conditions, then to apply step changes during day 3 in dilution rate(hydraulic shock) , or feed concentration(Quantitative shock) at the same organic mass loading rate. Performance was assessed in both the transient state and the new steady-state (post- shock). Shock load of different type did not produced equivalent disruptions of effluent quality for equal increases in mass loading rate. Based on effluent concentrations, a hydraulic and a Quantitative shock at the same mass loading caused equal increase in total effluent COD, but the increase was primarily a result of suspended solids the hydraulic shock and COD in the quantitative shock. The time which effluent COD came to peak values were about 32~48 hours at the low organic loads and 52 ~ 72 hours at the high organic loads, respectively A quantitative shock produced a much greater increase in effluent COD than did a hydraulic shock at the same mass loading. Mean and peak values of effluent concentration weve increased in 2.8~4.2 times at low organic loading rate, 5.2~6.6 times at the high organic loading rate, respectively. Key words : Aerobic fixed-film reactor, Quantitative shock, hydraulic shock, mass loading rate.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.21 no.3
• /
• pp.57-65
• /
• 2018

The study is conducted to establish the National Protected Areas Integrated System(NPAIS) which includes National Protected Areas(NPAs) and other conservation measures in terms of effective ways for biodiversity conservation. Additionally, it is carried out to evaluate progress toward quantitative expansion goals in Aichi biodiversity Target 11. The NPAIS consists of NPAs and other effective area-based conservation measures(OECMs). There are two different types of OECMs. OECMs type I, including water-source protection Areas(WPA), riparian zones(RZ), fishery-resource protection zones (FPZ), and urban natural park zones(UNPZ), is a potential protected area which is recommended to be incorporated into the NPAs for effective management. OECMs type II means development restriction zones(DRZ), natural recreation forests(NRF), and buffer zones for Korea national arboretum(BKNA). As a result of evaluating the quantitative expansion goals of the NPAIS, terrestrial and inland water protected areas exceed 17% of the quantitative goal in Aichi biodiversity Target 11. The larger the area of individual OECMs and the lower the degree of overlap with NPAs, the higher the contribution of them to the terrestrial and inland water protected areas. DRZ contributes to enlarge more than 3% of quantitative expansion. And RZ and NRF contribute more than 1%. The marine protected areas are expanded by $1,425km^2$ through FPZ, but the expanded area is very small as comparing with the total marine area. It adds only 0.321% to the quantitative expansion. The rest of OECMs also has very poor quantitative expansion contributions in the marine area. Consequently, the NPAIS is effective for quantitative expansion of land areas, but not for marine areas.