• 대한약침학회지
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• 제22권3호
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• pp.166-170
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• 2019

Objectives: Attentional and memory functions are important aspects of neural plasticity that, theoretically, should be amenable to pharmacopuncture treatments. A previous study from our laboratory suggested that quantitative electroencephalographic (qEEG) measurements of theta/beta ratio (TBR), an index of attentional control, may be indicative of academic performance in a first-semester medical school course. The present study expands our prior report by extracting and analyzing data on frontal theta and beta asymmetries. We test the hypothesis that the amount of frontal theta and beta asymmetries (fTA, fBA), are correlated with TBR and academic performance, thereby providing novel targets for pharmacopuncture treatments to improve cognitive performance. Methods: Ten healthy male volunteers were subjected to 5-10 min of qEEG measurements under eyes-closed conditions. The qEEG measurements were performed 3 days before each of first two block examinations in anatomy-physiology, separated by five weeks. Amplitudes of the theta and beta waveforms, expressed in ${\mu}V$, were used to compute TBR, fTA and fBA. Significance of changes in theta and beta EEG wave amplitude was assessed by ANOVA with post-hoc t-testing. Correlations between TBR, fTA, fBA and the raw examination scores were evaluated by Pearson's product-moment coefficients and linear regression analysis. Results: fTA and fBA were found to be negatively correlated with TBR (P<0.03, P<0.05, respectively) and were positively correlated with the second examination score (P<0.03, P=0.1, respectively). Conclusion: Smaller fTA and fBA were associated with lower academic performance in the second of two first-semester medical school anatomy-physiology block examination. Future studies should determine whether these qEEG metrics are useful for monitoring changes associated with the brain's cognitive adaptations to academic challenges, for predicting academic performance and for targeting phamacopuncture treatments to improve cognitive performance.

• 한국컴퓨터정보학회논문지
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• 제26권6호
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• pp.145-154
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• 2021

현재 추진하고 있는 교육부의 권한이 시·도교육청으로 배분되는 것을 환영하면서도 이것이 오히려 중앙 일개 기관에 집중되어 있던 권한을 현장에 더욱 가까운 지방 17개 기관으로 확대 증폭시키는 것이 되지 않을까 본인은 굉장히 우려하고 있다. 그간 학교교육을 저해하는 중요원인으로 교육부와 교육청이 공히 공동원인 제공자로 기능해 왔다. 교육부의 권한 이양은 기존 교육부의 기능과 역할의 양적 축소와 시·도 교육청 역할과 기능의 양적 확대로 귀결되는 순간 개악이 될 가능성이 높아진다. 학교자치에 대한 법적 장치도 마련되지 않은 현실에서 더욱이 수직적 관료행정 문화의 뿌리가 깊은 우리 교육계 풍토에서 학교자치 원칙을 강조하는 것은 말 뿐인 조치로 끝나거나 시늉내기 수준에서 멈춰버릴 가능성이 농후하다. 따라서 교육부의 권한을 넘겨받을 시·도교육청과 교육감의 권한을 견제할 수 있는 보다 안정적인 장치가 필요하다. 이는 학교자치가 법제화되어도 여전히 요구되는 제도이기도 하다. 따라서 독립적인 교육위원회 부활, 시·도 교육청에 지방교육위원회 설치, 주민참여제도(주민소환제, 주민직접발안제, 주민소송제, 주민투표제, 주민감사청구제도)의 활성화가 필요하다고 생각한다.

• 대한한의학회지
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• 제24권2호
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• pp.1-11
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• 2003

Objectives : The aim of this study was to characterize the effect of Injinchunggan-tang on $TGF-{\beta}1-induced$ hepatic fibrosis. Methods : mRNA and protein expression levels of $TGF-{\beta}1$ in Injinchunggan-tang-treated HepG2 cells were compared to untreated cells using quantitative RT-PCR and ELISA assay, respectively. mRNA expression levels of the TGF-1 pathway genes (TR-1, TR-II, Smad2, Smad3, Smad4, and PAI-1) and fibrosis-associated genes (CTGF, fibronectin, and collagen type 1) were evaluated by quantitative RT-PCR. The effect of Injinchunggan-tang on cell proliferation of T3891 human fibroblast was evaluated using [$^3H$]thymidine incorporation assay. Results : Expression of $TGF-{\beta}1$ mRNA and protein was inhibited by Injinchunggan-tang in a dose- and time-dependent manner. Whereas $TGF-{\beta}1-mediated$ induction of PAI-1 was suppressed by Injinchunggan-tang, expression of the $TGF-{\beta}1$ pathway genes such as TR-1, TR-II, Smad2, Smad3, and Smad4 was not affected by Injinchunggan-tang treatment. Injinchunggan-tang was found to inhibit $TGF-{\beta}1-induced$ cell proliferation of T3891 human fibroblast, and also abrogated $TGF-{\beta}1-mediated$ transcriptional up-regulation of CTGF, fibronectin, and collagen type I. Conclusions : This study strongly suggests that the liver cirrhosis-suppressive activity of Injinchunggan-tang may be derived at least in part from its inhibitory effect on $TGF-{\beta}1$ functions, such as blockade of $TGF-{\beta}1$ stimulation of fibroblast cell proliferation and fibrosis-related gene expression as well as expression of $TGF-{\beta}1$ itself.

• Mass Spectrometry Letters
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• 제1권1호
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• pp.25-28
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• 2010

The high-throughput identification and accurate quantification of proteins are essential strategies for exploring cellular functions and processes in quantitative proteomics. Stable isotope tagging is a key technique in quantitative proteomic research, accompanied by automated tandem mass spectrometry. For the differential proteome analysis of mouse neuronal cell lines, we used a multiplexed isobaric tagging method, in which a four-plex set of amine-reactive isobaric tags are available for peptide derivatization. Using the four-plex set of isobaric tag for relative and absolute quantitation (iTRAQ) reagents, we analyzed the differential proteome in several stroke time pathways (0, 4, and 8 h) after the mouse neuronal cells have been stressed using a glutamate oxidant. In order to obtain a list of the differentially expressed proteins, we selected those proteins which had apparently changed significantly during the stress test. With 95% of the peptides showing only a small variation in quantity before and after the test, we obtained a list of eight up-regulated and four down-regulated proteins for the stroke time pathways. To validate the iTRAQ approach, we studied the use of oxidant stresses for mouse neuronal cell samples that have shown differential proteome in several stroke time pathways (0, 4, and 8 h). Results suggest that histone H1 might be the key protein in the oxidative injury caused by glutamate-induced cytotoxicity in HT22 cells.

• 디지털융복합연구
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• 제12권11호
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• pp.407-414
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• 2014

본 연구의 목적은 컴퓨터 방사선영상에서 X-선 초점 크기와 디지털영상후처리에 따른 공간분해능을 정량적으로 분석하고자 하였다. 초점의 크기는 소초점(0.6 mm)와 대초점(1.2 mm)을 이용하였다. 공간분해능의 정량적 분석은 엣지 측정법의 변조전달함수(MTF)를 이용하였다. 디지털영상후처리는 다단계 이미지 대비 증폭 알고리즘을 이용하여 경계면 증강과 대조도 증강에 따른 50%와 10%의 MTF를 평가하였다. 그 결과 모든 초점에서 MTF 50%의 공간분해능이 대조도 증강보다 경계면 증강에서 유의하게 높았다. 또한 대초점에서 획득된 영상은 디지털영상처리를 통해 공간분해능이 향상되었다. 결론적으로 이러한 결과는 컴퓨터 방사선영상에서 골격계 및 흉부영상과 같은 고 공간분해능 임상영상을 얻기 위한 기초자료로 활용할 수 있다.

• Spatial Information Research
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• 제19권2호
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• pp.29-36
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• 2011

본 연구는 표면 파라미터 추정시 고려하는 주요 인자별로 각 조정모델들을 분류하고 그들의 추정정확도를 사전분석함으로써 이들 모델링 인자들이 각 대상파라미터의 추정에 주는 영향을 정량적으로 분석하였다. 현재 지표면형상에 대한 정보를 취득하기 위하여 라이다영상, 항공영상, SAR영상 등 다양한 자료가 활용되고 있고, 이들로부터 지표면 형상을 정량적으로 분석하기 위해서는 임의지점 주위의 관측값들을 이용하여 해당 지점의 형상을 구체적으로 파악하게 된다. 이러한 형상정보는 관측값 범위지정, 가중치방식, 그리고 수학적모델링 등 여러 인자들을 선정하여 산정할 수 있지만, 각 선정인자에 따라 표면의 형상정보는 다르게 산정되고 또한 그 정확도도 상이하게 된다. 따라서, 본 연구에서는 표면의 형상정보추출시 조정계산 인자들 따른 이러한 정확도를 비교함으로써 인자별 추정 정확도 변화 경향에 대한 진단을 실시하였다. 본 연구에서는 표면형상정보로 표고, 경사, 곡면의 2차계수를 대상으로 하고, 수학적함수, 커널크기, 가중유형별로 조정계산모델들을 구성하여 사전통계량을 계산하였고, 이에 따라 전통계량 변화를 비교 분석함으로써, 각 조정모델의 추정성능을 조정계산인자에 따라 정량적으로 비교분석하였다.

• Annals of Clinical Neurophysiology
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• 제2권1호
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• pp.8-12
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• 2000

Purpose : The rapid development and wide popularity of Digital EEG(DEEG) is due to its convenience, accuracy and applicability for quantitative analysis. These advantages of DEEG make one hesitate to use analog EEG(AEEG). To assess the advantage of DEEG system utilizing AEEG(DAEEG) over conventional AEEG and the clinical applicability, a DAEEG system was developed and applied to animal model Methods : Sprague-Dawley rat as status epilepticus model were used for collecting the EEG data. After four epidural electrodes were inserted and connected to 8-channel analog EEG(Nihon-Kohden, Japan), continous. EEG monitoring via computer screen was done from two rats simultaneously. EEG signals through analog amplifier and filters were digitized at digital signal processor and stored in Windows-based pentium personal computer. Digital data were sampled at a rate of 200 Hz and 12 bit of resolution. Acquisition software was able to carry out 'real-time view, sensitivity control and event marking' during continuous EEG monitoring. Digital data were stored on hard disk and hacked-up on CD-ROM for off-line review. Review system consisted of off-line review, saving and printing out interesting segment and annotation function. Results: This DAEEG system could utilize most major functions of DEEG sufficiently while making a use of an AEEG. It was easy to monitor continuously compared to Conventional AEEG and to control sensitivity during ictal period. Marking the event such as a clinical seizure or drug injection was less favorable than AEEG due to slowed processing speed of digital processor and central processing unit. Reviewing EEG data was convenient, but paging speed was slow. Storage and management of data was handy and economical. Conclusion : Relatively simple digital EEG system utilizing AEEG can be set-up at n laboratory level. It may be possible to make an application for clinical purposes.

• 한국발생생물학회지:발생과생식
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• 제21권4호
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• pp.379-389
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• 2017

Connexin (Cx) involves in the regulation of various physiological functions of tissue by forming a channel, a gap junction which allows direct cell-cell communication, between adjacent cells. The effect of a single subcutaneous treatment of estradiol benzoate (EB) or flutamide (Flu) at the weaning age on the expression of Cx isoforms in the adult caput epididymis was evaluated in this research. Using quantitative real-time PCR analysis, a low-dose of EB [$0.015{\mu}g/kg$ body weight (BW)] caused significant decreases of Cx30.3, Cx32, Cx40, Cx43, and Cx45 mRNA levels and no change of Cx26, Cx31, Cx31.1, Cx37 transcript levels. The treatment of a high-dose EB ($1.5{\mu}g/kg\;BW$) resulted in reduced expression of Cx30.3, Cx31, Cx43, and Cx45 but increased expression of Cx37 and Cx40. Expression of all Cx isoforms examined, except Cx31, was significantly increased by the treatment of a low-dose Flu ($500{\mu}g/kg\;BW$). However, the treatment of a high-dose Flu (5 mg/kg BW) led significant expressional suppression of Cx30.3, Cx31, Cx31.1, Cx32, Cx40, Cx43, and Cx45 but an increase of Cx37 transcript level. With the comparison of previous findings, the expression of Cx isoforms in the adult epididymis after the exposure to EB or Flu is likely differentially regulated in regional-specific and/or exposed postnatal age-specific manner.

• The Korean Journal of Physiology and Pharmacology
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• 제8권1호
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• pp.21-25
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• 2004

People in tropics have the ability to tolerate heat by residential permanence in the tropics. Previously, we have shown that African and Thai subjects who lived for whole their lives in only their respective countries sweat less under hot conditions than South Koreans who also lived whole their lives in Korea. The difference in sweating responses was attributed to the dissimilar central and peripheral sweating mechanisms operating in people from both groups. In the present study, acetylcholine (ACh), the primary transmitter for the sudomotor functions, was iontophoretically administered to South Koreans and Africans to determine the characteristic sudorific responses of their acclimatized biologic make-up to their respective environments. Using quantitative sudomotor axon reflex test (QSART), direct (DIR) and axon reflex (AXR) responses were evaluated. The findings revealed that the sweat onset-time among South Koreans was 0.91 min earlier than among Africans (P<0.01). The axon reflex sweat volume of nicotine receptor activity AXR(1) and sweat volume of muscarinic receptor activity DIR(2) among South Koreans were 79% and 53% greater (P<0.01), respectively. These results indicate that the reduced thermal sweating among Africans is at least in part attributed to the diminished sensitivity of sweat glands to ACh.

• Parasites, Hosts and Diseases
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• 제58권2호
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• pp.161-171
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• 2020

The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.