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Aberrant Expression of Cx Isoforms in the Adult Caput Epididymis exposed to Estradiol Benzoate or Flutamide at the Weaning

  • Lee, Ki-Ho (Dept. of Biochemistry and Molecular Biology, College of Medicine, Eulji University)
  • Received : 2017.12.01
  • Accepted : 2017.12.22
  • Published : 2017.12.31

Abstract

Connexin (Cx) involves in the regulation of various physiological functions of tissue by forming a channel, a gap junction which allows direct cell-cell communication, between adjacent cells. The effect of a single subcutaneous treatment of estradiol benzoate (EB) or flutamide (Flu) at the weaning age on the expression of Cx isoforms in the adult caput epididymis was evaluated in this research. Using quantitative real-time PCR analysis, a low-dose of EB [$0.015{\mu}g/kg$ body weight (BW)] caused significant decreases of Cx30.3, Cx32, Cx40, Cx43, and Cx45 mRNA levels and no change of Cx26, Cx31, Cx31.1, Cx37 transcript levels. The treatment of a high-dose EB ($1.5{\mu}g/kg\;BW$) resulted in reduced expression of Cx30.3, Cx31, Cx43, and Cx45 but increased expression of Cx37 and Cx40. Expression of all Cx isoforms examined, except Cx31, was significantly increased by the treatment of a low-dose Flu ($500{\mu}g/kg\;BW$). However, the treatment of a high-dose Flu (5 mg/kg BW) led significant expressional suppression of Cx30.3, Cx31, Cx31.1, Cx32, Cx40, Cx43, and Cx45 but an increase of Cx37 transcript level. With the comparison of previous findings, the expression of Cx isoforms in the adult epididymis after the exposure to EB or Flu is likely differentially regulated in regional-specific and/or exposed postnatal age-specific manner.

Keywords

References

  1. Arroteia KF, Garcia PV, Barbieri MF, Justino ML, Pereira LAV (2012) The epididymis: embryology, structure, function and its role in fertilization and infertility. In: Embryology-Updates and Highlights on Classic Topics. (Ed: L. A. V. Pereira) In Tech. Croatia pp.41-66.
  2. Atanassova N, McKinnell C, Williams K, Turner KJ, Fisher JS, Saunders PT, Millar MR, Sharpe RM (2001) Age-, cell- and region-specific immunoexpression of estrogen receptor alpha (but not estrogen receptor beta) during postnatal development of the epididymis and vas deferens of the rat and disruption of this pattern by neonatal treatment with diethylstilbestrol. Endocrinology 142:874-886. https://doi.org/10.1210/endo.142.2.7978
  3. Cyr DG, Hermo L, Laird DW (1996) Immunocytochemical localization and regulation of connexin 43 in the adult rat epididymis. Endocrinology 137:1474-1484. https://doi.org/10.1210/endo.137.4.8625926
  4. Dube E, Dufresne J, Chan PT, Cyr DG (2012) Epidermal growth factor regulates connexin 43 in the human epididymis: role of gap junctions in azoospermia. Hum Reprod 27:2285-2296. https://doi.org/10.1093/humrep/des164
  5. Goodenough DA, Paul DL (2009) Gap junctions. Cold-Spring Harb. Perspect. Biol 1:a003061.
  6. Han S-Y, Lee K-H (2013) The expression patterns of connexin isoforms in the rat caput epididymis during postnatal development. J Ani Sci Tech 55:249-255. https://doi.org/10.5187/JAST.2013.55.4.249
  7. Lawrence TS, Beers WH, Gilula NB (1978) Transmission of hormonal stimulation by cell-to-cell communication. Nature 272:501-506. https://doi.org/10.1038/272501a0
  8. Lee K-H (2013) Differential expression of multiple connexins in rat corpus and cauda epididymis at various postnatal stages. J Ani Sci Tech 55:521-530. https://doi.org/10.5187/JAST.2013.55.6.521
  9. Lee K-H (2015) Modification of gene expression of connexins in the rat corpus epididymis by estradiol benzoate or flutamide exposure at the early neonatal age. Dev Reprod19:69-77. https://doi.org/10.12717/DR.2015.19.2.069
  10. Lee K-H (2016a) Changes in expression of connexin isoforms in the caudal epididymis of adult Sprague-Dawley rats exposed to estradiol benzoate or flutamide at the neonatal Age. Dev Reprod 20:237-245. https://doi.org/10.12717/DR.2016.20.3.237
  11. Lee K-H (2016b) Effects of exposure to estradiol benzoate or flutamide at the weaning age on expression of connexins in the caudal epididymis of adult rat. Dev Reprod 20:349-357. https://doi.org/10.12717/DR.2016.20.4.349
  12. Lee K-H (2017) Expressional changes of connexin isoform genes in the rat caput epididymis exposed to flutamide or estradiol benzoate at the early postnatal age. Dev Reprod 21:317-325. https://doi.org/10.12717/DR.2017.21.3.317
  13. Lee S-K, Lee K-H (2015) Aberrant expression of connexin isoforms in the corpus epididymis of the adult rat by exposure to estradiol benzoate or flutamide at the weaning age. Dev Reprod 19:217-226. https://doi.org/10.12717/DR.2015.19.4.217
  14. Lydka M, Kopera-Sobota I, Kotula-Balak M, Chojnacka K, Zak D, Bilinska B (2011). Morphological and functional alterations in adult boar epididymis: Effects of prenatal and postnatal administration of flutamide. Acta Vet Scand 53:12. https://doi.org/10.1186/1751-0147-53-12
  15. Ma Y, Zhang K, Ren F, Wang J (2017) Developmental fluoride exposure influenced rat's splenic development and cell cycle via disruption of the ERK signal pathway. Chemosphere 187:173-180. https://doi.org/10.1016/j.chemosphere.2017.08.067
  16. Mese G, Richard G, White TW (2007) Gap junctions: basic structure and function. J Invest Dermatol 127: 2516-2524. https://doi.org/10.1038/sj.jid.5700770
  17. Robaire B, Hamzeh M (2011) Androgen action in the epididymis. J Androl 32:592-599. https://doi.org/10.2164/jandrol.111.014266
  18. Robaire B, Hermo L (1988) Efferent ducts, epididymis, and vas deferens: structure, functions, and their regulation. In: Knobil E et al (eds.). The Physiology of Reproduction. Raven Press, New York, NY, pp 999-1080.
  19. Robaire B, Hinton BT, Orgebin-Crist MC (2006) The epididymis. In: Knobil E and Neil J (eds.) The Physiology of Reproduction, Elsevier, New York, NY pp 1071-1148.
  20. Schulster M, Bernie AM, Ramasamy R (2016) The role of estradiol in male reproductive function. Asian J Androl 18:435-440. https://doi.org/10.4103/1008-682X.173932
  21. Seo H-H, Seon C-W, Choi I, Cheon Y-P, Cheon T-H, Lee K-H (2010) Expressional profiling of connexin isoforms in the initial segment of the male reproductive tract during postnatal development. Reprod Dev Biol 34:103-109.
  22. Serova LI, Harris HA, Maharjan S, Sabban EL (2010) Modulation of responses to stress by estradiol benzoate and selective estrogen receptor agonists. J Endocrinol 205:253-62. https://doi.org/10.1677/JOE-10-0029
  23. St-Pierre N, Dufresne J, Rooney AA, Cyr DG (2003) Neonatal hypothyroidism alters the localization of gap junctional protein connexin 43 in the testis and messenger RNA levels in the epididymis of the rat. Biol Reprod 68:1232-1240. https://doi.org/10.1095/biolreprod.102.010504
  24. Tan MH, Li J, Xu HE, Melcher K, Yong EL (2015) Androgen receptor: structure, role in prostate cancer and drug discovery. Acta Pharmacol Sin 36:3-23. https://doi.org/10.1038/aps.2014.18
  25. Valiunas V, Polosina YY, Miller H, Potapova IA, Valiuniene L, Doronin S, Mathias RT, Robinson RB, Rosen MR, Cohen IS, Brink PR (2005) Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions. J Physiol 568:459-468. https://doi.org/10.1113/jphysiol.2005.090985
  26. Watanabe Y, Nakajima K, Mizukami S, Akahori Y, Imatanaka N, Woo GH, Yoshida T, Shibutani M (2017) Differential effects between developmental and postpubertal exposure to N-methyl-N-nitrosourea on progenitor cell proliferation of rat hippocampal neurogenesis in relation to COX2 expression in granule cells. Toxicology 389:55-66. https://doi.org/10.1016/j.tox.2017.06.013
  27. You L, Sar M (1998) Androgen receptor expression in the testes and epididymides of prenatal and postnatal Sprague-Dawley rats. Endocrine 9:253-261. https://doi.org/10.1385/ENDO:9:3:253