• KSBB Journal
• /
• v.11 no.1
• /
• pp.92-98
• /
• 1996

A chitinase-producing bacterium was isolated from seashore mud around Beobseongpo in Chunnam province by selective enrichment culture, and among it, one isolate which was the best in producing of chitinase was selected. Nutrient or MacConkey medium was confirmed with secreting of prodigiosin pigment by Serratia sp. JM, and it was performed by the production of clear zone on medium containing chitin. Serratia sp. JM was almost same compared with Serratia marcescens ATCC 27117 in respect of its morphological, physiological and biochemical characteristics except succinic, urea and pyruvic acid. Serratia sp. JM was resistant to tetracycline but was not resistant to kanamycin and chloramphenicol. The optimal temperature and pH for the production of chitinase from Serratia sp. JM were $30^{\circ}C$ and 7.5, respectively. Production of chitinase and pH in the medium increased until the cultivation of 120 hours, but after 120 hours, they were decreased due to the acetic acid accumulated from degradation of chitin by Serratia sp. JM.

• Applied Biological Chemistry
• /
• v.3
• /
• pp.1-7
• /
• 1962

In order to study the rate control step in the protein metabolic course of the chlorophyll formation, the transaminase activities which are obtained freely in the extracts of cotyledons of germinating peas at the light and the dark places, are measured in Beckman mopel D.U, spectrophoto meter at 490 mu. In this case, of two enzymatic reaction products; oxalacetic acid and pyruvic acid, the former is converted to pyruvic acid by aniline citrate and after each pyruvate phenyl hydrazones are extracted by toluenes: when this is treated with strong alcoholic alkali, a colored hydrazone is formed and it is measured by above apparatus. The estimated G.O.T. and G.P.T. in the germinated cotyledons at dark and light places considerably differ in their activities; G.O.T. and G.P.T. activities which are formed at the light are more increased than at the dark and also they differ in their rates through germination, though G.O.T. activity increment is smoothly but that of G.P.T. is more sharply, and they are considered to be directly affected to the chlorophyll formation and indirectly to the growth. G.O.T. and G.P.T. in each fractions of cell in the cotyledons should be formed by dissociation of zymogens in the microsomal fractions and it seems to promoted by light. In the formation of the chlorophyll, the protein metabolism occurred mainly in the microsomal fractions and the rate determining step is found at the point where the zymogene that is able to produce G.P.T. is activated, and this activation is promoted by light as noted above.

• Journal of Bio-Environment Control
• /
• v.4 no.2
• /
• pp.144-151
• /
• 1995

This experiment was carried out to investigate the effect of different nutrient solution temperatures on the growth and quality of welsh onion in winter season. The fresh weight increased with the increase of temperature within the range of 13-23$^{\circ}C$. The top length was longer 18 and 23$^{\circ}C$ than at 13$^{\circ}C$, the dry weight was the highest at 18$^{\circ}C$. The potassium content in plant was the highest at 18$^{\circ}C$, calcium and magnesium contents were higher at 13 and 18$^{\circ}C$ than at 23$^{\circ}C$. The vitamin C content decreased with the increase of nutrient solution temperature, but there was no statistical significance within treatments. The pyruvic acid was higher at 13 and 18$^{\circ}C$ than at 23$^{\circ}C$, while nitrate content was lower at 13 and 18$^{\circ}C$ than 23$^{\circ}C$. Therefore, for growth and quality of welsh onion, it was concluded that 18$^{\circ}C$ may be suitable nutrient solution temperature in winter season.

• Journal of Bio-Environment Control
• /
• v.3 no.2
• /
• pp.99-105
• /
• 1994

The objective of this study was conducted for elucidation of effects of the NO$_3$$^{[-10]}$ -N. NH$_4$$^{+}$-N ratio in the nutrient solution on the growth and quality of welsh onion(Allium fistulosum L.). The pH of nutrient solution increased in NO$_3$$^{[-10]}$ -N : NH$_4$$^{+}$-N ratio of 9 : 1 treatment, decreased in 1 : 1, 1 : 3 treatment, but was stable in 3 : 1 treatment during cultivation. The apparent growth of welsh onion was best in the treatment of 9 : 1(NO$_3$$^{[-10]}$ -N : NH$_4$$^{+}$-N ratio), however the treatment of 1 : 3 resulted in poor growth. The NO$_3$$^{[-10]}$ -N content of the plants increased in proportion to that content of nutrient solution. Pyruvic acid content of welsh onion was highest at 9 : 1, 3 : 1 treatment, and lowest at 1 : 3 treatment.tment.

• Journal of Life Science
• /
• v.28 no.1
• /
• pp.1-8
• /
• 2018

To confirm the function of lactate dehydrogenase (LDH) (EC 1.1.1.27, LDH), its metabolism was studied by activity, kinetics, and isozyme analysis in tissues of Ldh testis-specific C expressing mice (Mus musculus) maintained in a state of starvation for 48 hr and 96 hr. In skeletal muscle, liver, and eye tissues, LDH and LDH $A_4$ activity increased and anaerobic metabolism predominated. While LDH activity in the heart and kidney tissues decreased, LDH $B_4$ activity increased and aerobic metabolism predominated, producing pyruvic acid. In the testis tissue, LDH $C_4$ activity decreased. In the brain tissue, LDH activity increased, but the isozyme change was small and the amount of pyruvic acid decreased. $K{_m}^{PYR}$ increased in tissues other than kidney tissue, and the affinity for pyruvic acid decreased. Consequently, in Ldh-A and B-expressing tissues, the activities of isozymes with higher concentrations increased. However, in Ldh-A, B, and C-expressing tissue, $C_4$ decreased and the function of the tissue also decreased. In particular, LDH in brain tissue played a role as a pyruvate reductase. Therefore, this process might be the mechanism for producing energy in the state of starvation.

• Journal of Bio-Environment Control
• /
• v.14 no.4
• /
• pp.254-262
• /
• 2005

This experiment was carried out to select the desirable welsh onion (Allium fistulosum L.) varieties for the year-round cultivation in water culture. Ten varieties were tested, of which five were Korean's and the other five were Japanese's. The germination percentage and germinative energy of most varieties we.e more than 90 percent. The fastest germination speed were 'Geumjanguedaepa', 'Gujopa' and 'Fuyune'. The best growth of the varieties were 'Geumjanguedaepa' and 'Tokyokuro' in spring, 'Geumjanguedaepa', 'Gujopa', 'Saesaeksisilpa' and 'Tokyokuro' in summer, 'Geumjanguedaepa', 'Gujopa' and 'Tokyokuro' in fall, and 'Geumjanguedaepa', 'Midorigawa' and 'Tokyokuro' in winter cultivation. Suitable varieties of growth fur all seasons were 'Geurnjanguedaepa' and 'Tokyokuro', though 'Gujopa' was good in summer and fall. In the view of nutritional content, nitrate content was significantly lower in 'Geumjanguedaepa', 'Tokyokuro' and 'Fuyune' than that in'Saesaeksisilpa' and 'Starziam'. Vitamin C content was higher in 'Aqua Green', 'Midorigawa' and 'Seoulbaekpa' than that of the others. Pyruvic acid content was the highest in 'Geumjanguedaepa' and 'Suckchanguedaepa'. Based on the above results, 'Geumjanguedaepa', 'Gujopa' and 'Tokyokuro' were selected as the most desirable young welsh onion varieties for the year-round water culture.

• Food Science and Preservation
• /
• v.7 no.3
• /
• pp.245-248
• /
• 2000

It was attempted to find a method to prevent greening of crushed garlic. The storage conditions and heat treatments before crushing were tested for the prevention of greening in crushed garlic. 'Namhae' garlic (sub-tropical type), 'Seosan' and 'Danyang' garlic (traditional) type for cool area) were stored in room temperature(20${\pm}5^{\circ}C$, low temperature(0$^{\circ}C$), and CA(O$_2\;3%,\;CO_2$ 5%) storage for five months, and their samples were crushed. The crushed garlic had no significant differences in greening according to the cultivars. Greening did not occur for the crushed garlic from bulbs stored either in room temperature or CA storage. Greening was the unique symptom observed in crushed garlic from bulbs stored in low temperature. For the prevention of greening, heat treatment was conducted at 40, 35 and 30$^{\circ}C$ prior to make the crushed garlic from bulbs which were stored in low temperature. The effective duration of heat treatment before crushing was 7 days at 30$^{\circ}C$, 3 days at 35$^{\circ}C$, and 1 days at 40$^{\circ}C$ Greening was most effectively prevented in 1 day at 40$^{\circ}C$ treatment. During the heat treatments, changes in enzymatic pyruvic acid content and sprouting rate were slightly observed in garlic bulbs treated at 40$^{\circ}C$ for 1 day.

• Korean Journal of Food Science and Technology
• /
• v.24 no.3
• /
• pp.272-278
• /
• 1992

Takju, a Korean traditional wine, was prepared by using nuruk and Koji which were inoculated with Aspergillus kawachii and Aspergillus shirousamii. Those chemical composition such as alcohols and organic acids were determined with gas chromatography to investigate the variation of its content by the fermentation. Alcohol such as methyl, ethyl, n-propyl, i-butyl, i-amyl alcohol were detected in the most takju mash samples. Alcohol contents in the groups fermented with KNR and SNR were slightly higher than KKR, SKR groups. Lactic acid were concentrated and organic acids such as pyruvic, oxalic, malonic, succinic, maleic, malic, ${\alpha}-keto$ glutaric acid were also detected in the most samples. The pH was lowest in the KKR group. The total acid content was slightly decreased at the later fermentation and was highest in KKR. The content of minerals were highest in the WNR and BNR groups. Throughout fermentation the content of potassium and magnesium varied greatly with the tested groups.

• Journal of the Korean Wood Science and Technology
• /
• v.38 no.1
• /
• pp.75-84
• /
• 2010

This study was performed to investigate change in chemical composition of Acer mono saps collected in Hamyang, Inje, Namyangju and Yeongwol depending on storing period. pH of A. mono sap was in the range of 4.43~5.68, and it was decreased rapidly with the increase of storing period. A. mono sap collected in Yeongwol in Feb. 22 contained 2.06% sucrose. Degradation of sucrose was occurred when storing period was extended, and it caused production of organic acid like pyruvic acid, lactic acid, acetic acid and ethanol. Detected minerals in A. mono sap were K, Ca, Na, Mg, P, Si, Al, Mn, Fe, Cu and Zn, however, K and Ca content reached 93%. A. mono sap collected in Inje in Mar. 03 contained 131.72 mg/${\ell}$, which was especially high K content among the A. mono sap. 1.55~3.50 mg/${\ell}$ of Ascorbic acid was found in the A. mono sap. Sap collected in early date was less degraded.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.30 no.6
• /
• pp.944-947
• /
• 1997

Docosahexaenoic acid (DHA) rich oil was obtained from blue fin tuna (Thunnus thynnus orientalis) orbital tissue with centrifugation of 12,000 rpm under vaccum $(10^{-1}\;Torr)\;at\;4^{\circ}C$. The effect of DHA rich oil (DHA content; $27.8\%$) on $CCl_4-induced$ acute injury was investigated biochemically and histopathologically. Dosage of DHA rich oil on 24h before $ CCl_4-administration$ prevented significantly the increase of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GTP) values. No necrosis of hepatocytes was observed in rat livers treated with DHA oil on 24h prior to $CCl_4-administration$. These results suggested that DHA oil controls the accumulation of fat in the liver and prevented the liver injury.