• 한국환경복원기술학회지
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• 제5권2호
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• pp.34-38
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• 2002

자생 양치식물중 실내식물로서 개발가치가 높은 가는쇠고사리(Arachniodes aristata )와 석위(Pyrrosia lingua)의 실내 습도조건에 따른 생육 반응을 살펴보고자 수행하였으며, 그 결과는 다음과 같다. 1. 가는고사리와 석위는 상대습도 50%, 90% 보다 25%와 같은 저습도시 식물체내수분함량, 엽록소함량, 관상가치가 저하되었다. 2. 상대습도에 대한 용토별 생육에 있어 고습도인 90%일시 용토별 생육에는 큰 차이가 나타나지 않았으나, 중습도인 50%일 경우 일반 토양이 가장 좋은 것으로 나타났다. 저습도인 25%의 경우 가는쇠고사리는 인공배합토에서, 석위는 일반토양에서 생육이 좋은 것으로 나타났다.

• 생약학회지
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• 제23권4호
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• pp.276-279
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• 1992

Two flavonoid glycosides, astragalin(l) and liquiritin(2), were isolated from the fronds of Pyrrosia lingua.

• Natural Product Sciences
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• 제20권4호
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• pp.285-289
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• 2014

Pyrrosia lingua has been widely used as a traditional medicine for the treatment of lots of diseases including pain management. However pharmacological and phytochemical studies on its anti-nociceptive properties are extremely limited. In this work, we investigated the effects of methanol extract of Pyrrosia lingua (MPL, 250 and 500 mg/kg) on the both of central and peripheral nociceptive pain. The results from tail-immersion test and hotplate test revealed that MPL has potent anti-nociceptive effects on thermal nociception. In addition, MPL efficiently reduced the acetic acid-induced chemical nociception compared to indomethacin. We also carried out formalin test and MPL reduced formalin-induced pain response on both phases, suggesting MPL has antinociceptive activities on the central and peripheral pain. In combination test using naloxone, anti-nocicpetive activity of MPL was reduced, indicating that MPL acts as a partial opioid receptor agonist. These results suggest that MPL may be possibly used as a valuable natural product-derived painkiller.

• 생약학회지
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• 제31권3호
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• pp.288-294
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• 2000

The Korean crude drug 'Suk Wi' has been used as the folk remedy for diuretic and gonorrhea. The botanical origin of the crude drug has never been studied pharmacognostically. To clarify the botanical origin of Suk Wi, studied on the morphological and anatomical characteristics of Pyrrosia species growing wild in Korea i.e., P. linearifolia, P. lingua, P. petiolosa, P. tricuspis and Suk Wi from Korea on Korean market. As a result, it was made clear that Suk Wi from Korea was derived from Pyrrosia lingua and P. petiolosa.

• Journal of Applied Biological Chemistry
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• 제63권3호
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• pp.181-188
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• 2020

In this study, Tongue fern (Pyrrosia lingua) plants that have been used traditionally as medicines. Their traditional medicinal uses, regions where indigenous people use the plants, parts of the plants used as medicines. This study was designed to assess the antioxidant and inhibition activities of extracts from P. lingua. In the P. lingua extracts was measured ethanol activity, 80.0% ethanol was high activity. The antioxidant activity was measured in 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), assays. DPPH and ABTS radical in this experiment, solid and phenolic of extract were tested, but only an average concentration of 100 ㎍/mL was used. However, the phenolic extract is shown phenolic activity reached a peak. Also, phenolic extracts ware reached peak water and ethanol extracts. As a result, using the phenolic extracts did other antioxidant assays such as DPPH, ABTS, protection factor, and thiobarbituric acid reactive substances at 50-200 ㎍/mL concentrations. The activity of elastase and collagenase, inhibiting their activities may retard skin aging. α-Glucosidase and α-amylase, inhibitors need to be explored for the benefit of postprandial hyperglycemia in diabetic patients. Activities of tyrosinase, hyaluronidase and xanthine oxidase inhibitors of these enzymes are increasingly important ingredients in cosmetics and medications to protect the skin against hyperpigmentation and skin aging. Inhibition effects were investigated using the P. lingua extracts at 50-200 ㎍/mL concentrations. The expression levels of enzyme inhibitions activities were decrease in dependent-concentrations manner when P. lingua extracts were treated.

• 생약학회지
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• 제48권3호
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• pp.179-186
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• 2017

Pyrrosia lingua which belongs to Polypodiaceae has been used as a traditional medicine for the treatment of urinary system inflammation, urination disorder, and bronchitis. However, there are not enough phytochemical and pharmacological studies of P. lingua up to now. Here in this study, the protective effect of MeOH extract of whole plant of Pyrrosia lingua (MPL) against 2% glucose-induced toxicity was investigated using Caenorhabditis elegans (C. elegans) model system. We found that MPL significantly extended the lifespan of wild-type nematode under normal culture condition. MPL also effectively recovered the decreased lifespan caused by 2% glucose-toxicity. In addition, MPL efficiently attenuated the increased glucose concentration inside of nematode. Further studies evaluating diabetes-related factors revealed that MPL reduced both intracellular ROS and lipid accumulation which were up-regulated under 2% glucose supplement condition. Our data also showed that MPL improved the 2% glucose-induced shortened body movement of nematode. Lastly, we carried out genetic studies using several single gene knockout mutants to establish the possible target of MPL. Our results demonstrated that genes such as daf-2 and daf-16 were responsible for the protective activity of MPL against 2% glucose-induced toxicity. These results indicate that MPL exerts protective action against 2% glucose via regulation of insulin/IGF-1 sinaling pathway and FOXO activation.

• The Korean Journal of Ecology
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• 제12권3호
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• pp.145-160
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• 1989

Daehuksan Island (125°23'-125° 28' E and 34°37'-34°42' N, ca 19.7㎢) in the southwestern part of Mokpo, Korea, is a part of the Dadohae National Marine Park. Most of the actual vegetation of the island is substitutional vegetation under various human impact such as grazing, cutting for firewood. And natural vegetation is partially distributed in the tutelary shrine forests, deep valleys, etc. During 1987, phytosociological investigation of this was carried out on the basis of Braum-Blanguet's method. The forest vegetation was classified into 1 association and 7 communities, Camellia japonica, Machilus thunbergii, Ardisio-Castanopsietum sieboldii, Daphniphyllum macropodum-Quercus acuta, Pyrrosia lingua-Pinus densiflora. Rhododendron mucronulatum-Pinus thunbergii, Carpinus coreana, and Psedosasa japonica community. Grassland vegetation was classified into Gleichenia japonica, Gleichenia dichotoma, Arundinella hirta-Miscanthus sinensis, Artemisia princeps var. orientalis. Imperata cylindrica var. koenii Artemisia princeps var. orientalis-Pennisetum alpecuroides, Carex boottiana, and salt marsh vegetation. Thypa orientalis, Spergularia marina, Phragmites communis, Carex scabrifolia, and Triglochin maritimum community. Based on them, the actual vegetation map was made in 1:50,000 scale.

• 한국자원식물학회지
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• 제26권4호
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• pp.411-416
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• 2013

본 연구는 자생 양치식물 9종의 성엽과 근경을 재료로 ${\alpha}$-glucosidase 억제활성을 분석하여 천연 ${\alpha}$-glucosidase 저해제로서 개발 가능한 식물 소재를 선발하기 위하여 수행하였다. 수확된 성엽과 근경은 수세 후 동결건조 하였으며, 건조시료를 분쇄하여 100% 메탄올 용매로 30분 동안 초음파 추출을 하였고, 추출 후 감압여과 하여 ${\alpha}$-glucosidase 억제활성을 측정하였다. 양성 대조구로는 acarbose를 사용하였다. 추출물 $50{\mu}L$에 0.7 unit ${\alpha}$-glucosidase 효소액 $100{\mu}L$를 넣고 혼합하여 $37^{\circ}C$에서 10분간 반응시킨 후, 1.5 mM ${\rho}$-NPG 기질용액을 $50{\mu}L$ 넣고 $37^{\circ}C$에서 20분간 반응시켰다. 1 M $Na_2CO_3$ 1 mL로 반응을 정지시키고 405 nm에서 흡광도를 측정하였다. 회귀분석을 이용하여 0.7 unit ${\alpha}$-glucosidase 용액의 활성을 50%를 억제하는데 필요한 시료의 농도($IC_{50}$ 값)를 구하였다. 양치식물 9종의 ${\alpha}$-glucosidase 억제활성은 성엽($IC_{50}=14.00{\sim}913.33{\mu}g{\cdot}mL^{-1}$)과 근경 추출물($IC_{50}=12.93{\sim}205.84{\mu}g{\cdot}mL^{-1}$)에서 공히 acarbose($IC_{50}=1413.70{\mu}g{\cdot}mL^{-1}$)에 비해 높았다. 양치식물의 추출물은 acarbose에 비해 성엽은 1.55~100.98배, 근경은 6.87~109.33배 높은 것으로 나타났다. 특히 성엽에서는 석위의 억제활성이, 근경에서는 꿩고비의 ${\alpha}$-glucosidase 억제활성이 가장 높았다. ${\alpha}$-Glucosidase 활성의 50%를 억제하기 위한 성엽과 근경의 필요 생체량은 공히 꿩고비(각 0.35, 0.27 mg)에서 가장 적은 것으로 나타났다. 성엽의 경우는 석위의 $IC_{50}$ 값이 가장 높았으나 가용성 고형분의 함량이 꿩고비에 비해 2.4배 낮아, 오히려 꿩고비의 경제성이 더 높은 것을 알 수 있었다. 본 연구의 결과 꿩고비는 적은 생체량으로도 높은 ${\alpha}$-glucosidase 억제활성을 나타내기 때문에 경제적인 천연 ${\alpha}$-glucosidase 저해제 소재로써 개발 가치가 매우 높은 것을 알 수 있었다.

• 한국자원식물학회지
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• 제20권2호
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• pp.185-192
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• 2007

8종의 식용 및 약용 양치식물에서 80% 에탄올을 이용하여 부위별 추출물을 조제한 후 각각의 생리활성물질 및 항산화활성을 조사하였다. 총 폴리페놀 함량은 쇠고비의 근경에서 $57.32mg{\cdot}g^{-1}$로 가장 높았고, 그 다음은 쇠고비의 뿌리>석위 지상부>야산고비 지상부의 순으로 나타났다. 총 플라보노이드 함량은 야산고비의 지상부에서 $27.51mg{\cdot}g^{-1}$로 가장 높았고 야산고비 근경에서 $1.95mg{\cdot}g^{-1}$로 가장 낮았다. DPPH 및 ABTS 라디칼 소거능은 쇠고비의 뿌리 및 근경 추출물에서 가장 높게 조사되었다. 부처손 지하부와 고비 지상부를 제외한 모든 추출물에서 linoleic acid의 산화를 87% 이상 강하게 억제할 수 있는 것으로 확인되었다.

• 동의생리병리학회지
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• 제30권1호
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• pp.40-46
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• 2016

Many medicinal plants have been used for the treatment of edema, jaundice, and gonorrhea in traditional Oriental medicine. This screening study was designed to search the positive inotropic effects of herbal extracts in beating rabbit atria. Aquarius extracts of twenty six herbs were examined in atrial mechanical dynamics such as pulse pressure and stroke volume and atrial natriuretic peptide (ANP), one of the main hormones involved in the regulation of the body fluid and blood pressure homeostasis in perfused beating rabbit atria. Sophora flavescens Ait., Rheum officinale Baill., Acorus gramineus Sol., Chelidonium majus L., Pulsatilla koreana Nakai., Reynoutria japonica Houtt., Euphorbia lathyris L., Pyrrosia lingua (Thunb.) Farwell, Poncirus trifoliata Rafin., Anemarrhena asphodeloides Bunge, Kochia scoparia Schrad. significantly increased stroke volume and pulse pressure. However, those herbal extracts were not induced ANP secretion. We clarified the eleven herbal extracts for the positive inotropic effect independent of ANP secretion in beating rabbit atria. Thus these results provide a beneficial data for the treatment of the impairment of body fluid and blood pressure in traditional Oriental medicine.