Screening for Antioxidative Activities of Extracts from Aerial and Underground Parts of Some Edible and Medicinal Ferns

식용 및 악용 양치식물에서 부위별 추출물의 항산화활성 탐색

  • Jeong, Jin-A (Dept. of Horticultural Science & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kwon, Su-Hyun (Dept. of Horticultural Science & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee, Cheol-Hee (Dept. of Horticultural Science & Research Center for Bioresource and Health, Chungbuk National University)
  • 정진아 (충북대학교 원예과학과 & 생물건강산업개발연구센터) ;
  • 권수현 (충북대학교 원예과학과 & 생물건강산업개발연구센터) ;
  • 이철희 (충북대학교 원예과학과 & 생물건강산업개발연구센터)
  • Published : 2007.04.30

Abstract

The different parts of 8 edible and medicinal ferns were extracted with 80% ethanol and the bioactive substances and antioxidant activities were investigated. Total polyphenol content was highest in Cyrtomium fortunei rhizome ($57.32mg{\cdot}g^{-1}$) and followed by C. fortunei root, Pyrrosia lingua aerial part and Onoclea sensibilis var. interrupta aerial part. Total flavonoid contents ranged from 1.95 (Onoclea sensibilis var. interrupta rhizome) to $27.51mg{\cdot}g^{-1}$ (O. sensibilis var. interrupta aerial part). Among the samples tested, C. fortunei root and rhizome were found to be most effective in both DPPH and ABTS radical scavenging assay. Except in Selaginella tamariscina underground part and Osmunda japonica aerial part, most of the tested samples showed strong potential (above 87%) in inhibition rate on linoleic acid oxidation.

8종의 식용 및 약용 양치식물에서 80% 에탄올을 이용하여 부위별 추출물을 조제한 후 각각의 생리활성물질 및 항산화활성을 조사하였다. 총 폴리페놀 함량은 쇠고비의 근경에서 $57.32mg{\cdot}g^{-1}$로 가장 높았고, 그 다음은 쇠고비의 뿌리>석위 지상부>야산고비 지상부의 순으로 나타났다. 총 플라보노이드 함량은 야산고비의 지상부에서 $27.51mg{\cdot}g^{-1}$로 가장 높았고 야산고비 근경에서 $1.95mg{\cdot}g^{-1}$로 가장 낮았다. DPPH 및 ABTS 라디칼 소거능은 쇠고비의 뿌리 및 근경 추출물에서 가장 높게 조사되었다. 부처손 지하부와 고비 지상부를 제외한 모든 추출물에서 linoleic acid의 산화를 87% 이상 강하게 억제할 수 있는 것으로 확인되었다.

Keywords

References

  1. Adam, K.P. 1999. Phenolic constituents of the fern Phegopteris connectilis. Phytochemistry 52: 929-934 https://doi.org/10.1016/S0031-9422(99)00326-X
  2. Basile, A., V. Spagnuolo, S. Giordano, C. Sorrentino, A. Lavitola and R. Castaldo-cobianchi. 1997. Induction of antibacterial activity by ${\alpha}$-Doligogalacturonides in Nephrolepis sp. (Pteridophyta). Int. J. Antimicrobial Agents 8: 131-134 https://doi.org/10.1016/S0924-8579(96)00365-2
  3. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26: 1198-1204
  4. Cha, C.C., S.K. Lee, H.W. Lee, E. Lee, M.Y. Choi, T.J. Rhim and H.J. Park. 1997. Antioxidant effect of domestic plants. Kor. J. Pharmacogn. 28: 15-20
  5. Cho, Y.C., Y.B. Han and K.H. Shin. 2001. Screening for antioxidant activity of edible plants. J. Kor. Soc. Food Sci. Nutr. 30: 133-137
  6. Choi, S.Y., S.H. Lim, J.S. Kim, T.Y. Ha, S.R. Kim. K.S. Kang and I.K. Hwang. 2005. Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plants. Kor. J. Food Sci. Technol. 37: 549-556
  7. Choi, U., D.H. Shin, Y.S. Chang and J.I. Shin. 1992. Screening of natural antioxidant from plant and their antioxidative effect. Kor. J. Food Sci. Tech. 24: 142-148
  8. Choi, Y.M., M.H. Kim, J.J. Shin, J.M. Park and J.S. Lee. 2003. The antioxidant activities of the some commercial teas. J. Kor. Soc. Food Sci. Nutr. 32: 723-727 https://doi.org/10.3746/jkfn.2003.32.5.723
  9. Chung, I.M., K.H. Kim and J.K. Ahn. 1998. Screening of Korean medicinal and food plants with antioxidant activity. Kor. J. Med. Crop Sci. 6: 311-322
  10. Gombau, L., F. Garcia, A. Lahoz, M. Fabre, P. Roda-Navarro, P. Majano, J.L. Alonso-Lebrero, J.P. Pivel, J.V. Castell, M.J. Gomez- Lechon and S. Gonzalez. 2005. Polypodium leucotomos extract: Antioxidant activity and disposition. Toxicology in Vitro 20: 464- 471 https://doi.org/10.1016/j.tiv.2005.09.008
  11. Haraguchi H., K. Hashimoto and A. Yagi. 1992. Antioxidative substances in leaves of Polygonum hydropiper. J. Agric. Food Chem. 40: 1349-1351 https://doi.org/10.1021/jf00020a011
  12. Jayaprakasha, G.K., T. Selvi and K.K. Sakariah. 2003. Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Res. Int. 36: 117-122 https://doi.org/10.1016/S0963-9969(02)00116-3
  13. Jung, S.J., J.H. Lee, H.N. Song, N.S. Seong, S.E. Lee and N.I. Baek. 2004. Screening for antioxidant activity of medicinal plant extracts. J. Kor. Soc. Appl. Biol. Chem. 47: 135-140
  14. Kim, E.Y., I.H. Baik, J.H. Kim, S.R. Kim and M.R. Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Kor. J. Food Sci. Tech. 36: 333-338
  15. Kimura, T., M. Suzuki, M. Takenaka, K. Yamagishi and H. Shinmoto. 2004. L-O-Caffeoylhomoserine from Matteuccia struthiopteris. Phytochemistry 65: 423-426 https://doi.org/10.1016/j.phytochem.2003.10.008
  16. Lee, S.E., N.S. Seong, J.K. Bang, C.G. Park, J.S. Sung and J. Song. 2003. Antioxidant activities of Korean medicinal plants. Kor. J. Med. Crop Sci. 11: 127-134
  17. NFRI. 1990. Manuals of quality characteristic analysis for food quality evaluation (2). National Food Research Institute, Tsukuba, Japan. pp. 61
  18. Park, S.H. and K.S. Kim. 2004. Isolation and identification of antioxidant flavonoids from Salicornia herbacea L. J. Kor. Soc. Appl. Biol. Chem. 47: 120-123
  19. Reddy, V.L.N., V. Ravikanth, T.P. Rao, P.V. Diwan and Y. Venkateswarlu. 2001. A new triterpenoid from the fern Adiantum lunulatum and evaluation of antibacterial activity. Phytochemistry 56: 173-175 https://doi.org/10.1016/S0031-9422(00)00334-4
  20. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice- Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol. & Med. 26: 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  21. Song, J.C., N.K. Park, H.S. Hur, M.H. Bang and N.I. Baek. 2000. Examination and isolation of national antioxidants from Korean medicinal plants. Kor. J. Med. Crop Sci. 8: 94-101
  22. Soong, Y.Y. and P.J. Barlow. 2004. Antioxidant activity and phenoleic content of selected fruit seeds. Food Chem. 88: 411-417 https://doi.org/10.1016/j.foodchem.2004.02.003
  23. Velioglu Y.S., G. Mazza, L. Cao and B.D. Oomah. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric. Food Chem. 46: 4113-4117 https://doi.org/10.1021/jf9801973
  24. Wang, M.F., Y. Shao, J.G. Li, N.Q. Zhu, M. Rangarajan, E.J. Lavoie and C.T. Ho. 1998. Antioxidative phenolic compounds from sage (Salvia officinalis). J Agric. Food Chem. 46: 4869-4873 https://doi.org/10.1021/jf980614b