• 상하수도학회지
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• 제31권2호
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• pp.177-186
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• 2017

This study concerned the analysis on the efficiency of the conversion of water tank type supply system to direct water supply system to examine the feasibility of the conversion, as well as the calculation of optimal conversion range that enables the supply of safe, high-quality water at stable pressure in accordance with the standards of water supply facility. The results of this research showed that when converting water supply system from water tank type supply system to direct water supply system, more nodal points could be properly converted and more reduction of electricity usage was expected in case water pressure rather than residence time was fixed. This means that higher efficacy can be obtained by fixing water pressure when converting water supply system. However, since the number of the locations that received on-spot inspection was small and the electricity usage measured was not exclusively by water supply facility, it is difficult to judge that such reduction of electricity usage accurately represents reduced electricity usage by water supply facility alone. therefore, after having secured on-spot information about a larger number of locations in apartment complexes that have converted water supply system, and utilizing information about electricity usage exclusively by water supply facility, the proposed method of this research could be applied to accurately deducing expected reduction of electricity usage by water supply facilities of various other apartment complexes. It is also considered possible to deduce an effective operation method of water supply system by finding out an area that shows low pressure or low residual chlorine concentration in the optimal conversion range of water supply, followed by estimating the proper location of pumping station or the proper chlorine dosage at the power purification plant that supply water to the target area.

• KSBB Journal
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• 제24권5호
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• pp.469-476
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• 2009

The antioxidant activities of two crude extracts ($CH_2Cl_2$ and MeOH) and their solvent fractions (n-hexane, 85% aq. MeOH, n-BuOH, and $H_2O$ fractions) from Corydalis heterocarpa were determined by evaluating authentic $ONOO^-$ and $ONOO^-$ generated from SIN-1 (3-morpholinsydnonimine) in vitro as well as by measuring the degree of occurrence of intracellular reactive oxygen species (ROS) and nitric oxide (NO). Scavenging activities of solvent fractions on authentic $ONOO^-$ increased in the order of n-BuOH > 85% aq. MeOH > $H_2O$ > n-hexane fractions, while those on $ONOO^-$ generated from SIN-1 increased in the order of n-BuOH > 85% aq. MeOH > $H_2O$ > n-hexane fractions. In addition, all solvent fractions effectively inhibited the intracellular ROS and NO levels. The n-BuOH fraction especially exhibited the strongest ROS scavenging effect. Further purification of n-BuOH fraction led to the isolation of cnidimoside A, which presented the potent ROS scavenging effect at $10\;{\mu}M$. From these results, extracts of C. heterocarpa and its component, cnidimoside A, were predicted to be potentially useful as ingredients for protecting against oxidation.

• 환경영향평가
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• 제7권2호
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• pp.53-64
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• 1998

This study was performed to build up the ecological guidelines to grasp the structure of the vegetation change which is due to river rehabilitation. Anyway, river ecosystem and function has been destroyed owing to river development. It is important that river vegetation supplies ecological corridor and biotope. Two survey sites(Wonsungcheon and Pungseocheon)were investigated in the aspect of plant ecosystem and structure to settle the practical concept of river ecosystem. Each survey site was subdivided to five plots. The site was surveyed through the belttransect method. Wonsungcheon gets more seriously polluted as it runs to the urban area. In other words, there are On the other hand, Pungseocheon has more naturality but its downstream is under the pressure of various wood plants in the upstream area, but downstream area is dominated by naturalized plants such as Bidens frondosa, Panicum dichotomiflorum, etc. Riverbank of downstream has been changed into farm and parking lot. development. It should be preserved definitely because it still has abundant naturality and wetland which formed a biotope. The objective of the research is to find out the river retrogression and maintenance methods based on the riparian vegetation structure. To manage the river ecologically, hydrophytes should be induced partly for natural purification after the riverside is rehabilitated. The vegetation should be induced step by step to restore natural river and steady monitoring and research are required.

• 생약학회지
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• 제31권3호
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• pp.273-279
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• 2000

Leukotriene $B_4\;(LTB_4)$ is a pro-inflammatory mediator synthesized in myeloid cells from arachidonic acid. Elevated levels of $LTB_4$ have been found in a number of inflammatory diseases and levels are related to disease activity in some of these. Because $LTB_4$ interacts with cells through specific cell surface receptors, $LTB_4$ receptor blockade is the most specific approach to reduce the pathogenic role of $LTB_4$. In order to find $LTB_4$ receptor antagonist from plants, we screened the $LTB_4$ receptor antagonistic activity of the methanol extract and solvent fractions of herbal drugs. The ability of samples to inhibit specific binding of $[^3H]-LTB_4$ to human peripheral neutrophils was used as assay to evaluate the antagonistic activity of plant materials. Among the tested methanol extracts of herbal drugs, Mori Radicis Cortex, Perillae Semen, Armeniacae Semen and Sophorae subprostratae Radix showed potent inhibitory activity above 70% at the concentration of $100\;{mu}g/ml$. The inhibitory activities of $LTB_4$ binding to human neutrophils were evaluated for several solvent fractions at three different concentrations. Especially, hexane soluble fractions of Anemarrhenae Rhizoma and Embeliae Radix, and ethyl acetate soluble fractions of Aristolochiae Fructus, Magnoliae Cortex and Zingiberis Rhizoma crudus showed moderate activity at $25\;{mu}g/ml$. These fractions were promising candidates for the study of the activity-guided chromatographic purification of active compounds. Silica gel column chromatography of hexane soluble fractions of Anemarrhenae Rhizoma and Embeliae Radix gave very active sub-fractions, AA-4 and ES-4, and their inhibition activities of $LTB_4$ binding to human neutrophil at $30\;{mu}g/ml$ were 78% and 62%, respectively. From these results we could anticipate new $LTB_4$ receptor antagonist from herbal drugs, and the block of $LTB_4$ effects may provide beneficial in neutrophil mediated diseases such as inflammation and bronchial asthma.

• Natural Product Sciences
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• 제21권3호
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• pp.162-169
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• 2015

Hamamelis japonica (Hamamelidaceae), widely known as Japanese witch hazel, is a deciduous flowering shrub that produces compact clumps of yellow or orange-red flowers with long and thin petals. As a part of our ongoing search for phenolic constituents from this plant, eleven phenolic constituents including six flavonol glycosides, a chalcone glycoside, two coumaroyl flavonol glycosides and two galloylated compounds were isolated from the flowers. Their structures were elucidated as methyl gallate (1), myricitrin (2), hyperoside (3), isoquercitrin (4), quercitrin (5), spiraeoside (6), kaempferol 4'-O-β-glucopyranoside (7), chalcononaringenin 2'-O-β-glucopyranoside (8), trans-tiliroside (9), cis-tiliroside (10), and pentagalloyl-O-β-D-glucose (11), respectively. These structures of the compounds were identified on the basis of spectroscopic studies including the on-line LCNMR-MS and conventional NMR techniques. Particularly, directly coupled LC-NMR-MS afforded sufficient structural information rapidly to identify three flavonol glycosides (2 - 4) with the same molecular weight in an extract of Hamamelis japonica flowers without laborious fractionation and purification step. Cytotoxic effects of all the isolated phenolic compounds were evaluated on HCT116 human colon cancer cells, and pentagalloyl-O-β-D-glucose (11) was found to be significantly potent in inhibiting cancer cell growth.

• Molecules and Cells
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• 제28권1호
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• pp.19-24
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• 2009

The goal of this study was to create a novel baculovirus expression system that does not require recombinant virus purification steps. Transfection of insect cells with transfer vectors containing barnase under control of the Cotesia plutellae bracovirus (CpBV) promoters ORF3004 or ORF3005 reduced cell growth. Co-transfection with bApGOZA DNA yielded no recombinant viruses and nonrecombinant backgrounds. To further investigate the detrimental effects of barnase on insect cells, two recombinant bacmids harboring the barnase gene under control of the CpBV promoters, namely bAcFast-3004ProBarnase and bAcFast-3005ProBarnase, were constructed. While no viral replication was observed when only the recombinant bacmids were transfected, recombinant viruses were generated when the bacmids were co-transfected with the transfer vector, pAcUWPolh, through substitution of the barnase gene with the native polyhedrin gene by homologous recombination. Moreover, no non-recombinant backgrounds were detected from unpurified recombinant stocks using PCR analysis. These results indicate that CpBV promoters can be used to improve baculovirus expression vectors by means of lethal gene expression under the control of these promoters.

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2005년도 춘계 학술대회
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• pp.145-150
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• 2005

방사성 폐기물을 처분장에 처분하기 위해서는 처분 안전성을 확보하여야 한다. 본 연구는 간접측정 방법 중 하나인 물질수지 기법을 이용하여 방사성폐기물의 핵종재고량 평가 프로그램을 개발하였다. 개발 기법의 현장 적용평가를 위하여 고리4호기(9차계획예방정비)를 대상으로 선정하였다. 개발한 평가방법의 검증을 위해 정지수화학처리시 정화계통 내 핵종 제거량 평가자료를 바탕으로 비교평가를 수행하였다. 평가대상 핵종은 Co-60이며, 평가결과 상대오차 $1\%$미만으로 나타났다. 이와 같은 평가결과를 바탕으로 상용원전에서 제시하고 있는 해당기간 발생된 폐기물의 직접 측정 결과와 비교하였고, 그 결과 직접측정 방법에 의한 Co-60의 함유량은 본 연구의 개발기법에서 산출한 값보다 약 $50\%$ 작은 것으로 평가 하였다.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.296-304
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• 1994

Antibiotic substances were produced by Bacillus subtilis YB-70, a potential biocontrol agent found to suppress root-rot of eggplant (Solanum melonggena L) caused by Fusarium solani, in a dextrose glutamate medium and isolated by isoelectric precipitation. Partial purification was performed by column chromatography on silica gel with two solvent systems: chloroform-methanol and methanol-chloroform-water as eluting solvents, This active fraction YBS-1 s contained antifungal activity were soluble in ethanol, methanol, and water, but were not soluble in other solvents including acetone, butanol, ethyl ether, dimethylformamide, propanol, and etc. High performance liquid chromatography and thin layer chromatographic separation of YBS-1s showed that they have been composed of three biological active bands that were named YBS-1A, -1B, and -1C. The substances were stable to heat and resistant to protease. YBS-1s were active against a wide range of plant pathogenic fungi but did not inhibit the growth of bacteria and yeasts. They were not only fungicidal but also fungistatic against chlamydospores of F. solani. The $ED_{50}$ values for the chlamydospore germination and the germ-tube growth of F. solani were $O.725\mu\textrm{m}/ml\;and\;O.562\mu\textrm{m}/ml$, respectively. Microscopic observations proved the substances restricted the growth of phytopathogenic fungus F. solani by spore burst followed by dissolving of its germ-tube, and caused abnormal hyphal swelling after application to chlamydospores or growing hyphae. Cultural filtrate of B; subtilis YB-70 also suppressed the development of root-rot of eggplant in pot tests.

• 한국세라믹학회지
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• 제51권4호
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• pp.317-323
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• 2014

People could not imagine living without tab water. However, the water filtering process at a purification plant used to produce tab water creates tons of sludge, which is generally wasted. This sludge waste consists of (1) mineral elements, such as sand, (2) organic materials, and (3) a coagulant, which agglomerates the two. As an enormous amount of sludge waste is generated every year, numerous studies have been carried out to identify how to deal with this problem. Currently, however, most of the sludge waste is directly discarded in landfills. In the present study, water-purified sludge waste received a heat treatment at $1300^{\circ}C$ and was then ground into particles to be used as a ceramic material. Next, the resultant particles were compounded with chamotte substitutes to produce grogged clay that is suitable for wheel-throwing ceramics. Consequently, the plasticity of the sludge waste decreased as the content of calcination increased. Thus, it is considered that wheel throwing is available only up to PBF-3. Thus, it is available for wheel throwing and has a high strength of 864 $kgf/cm^2$ with less than 0.2 percent of porosity and absorption ratio were displayed in PBF-2 at $1280^{\circ}C$ with 20 percent of calcination from the purified sludge. Therefore, the PBF-2 body produced in this study was considered to be capable of replacing grogged clay in the market.

• 대한본초학회지
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• 제23권3호
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• pp.27-32
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• 2008

Objectives : In this paper, we describe that $^1H-NMR$ spectroscopy may be superior to the conventional HPLC for the quantitative analysis of hesperidin from Citrus unshiu. Methods : $^1H-NMR$ spectra (400 MHz) were recorded in $DMSO-d_6$ using a Varian UNITY Inova AS 400 FT NMR spectrometer. One hundred milligram of powdered Citrus unshiu was weighed out and mixed with 1 ml of $DMSO-d_6$ with sonication for 30 min (room temperature). The extracts were filtrated through a 0.45 ${\mu}m$ PVDF filter and 0.5 ml of filtrated extract used for quantitative $^1H-NMR$ measurement (added 1 mg of dimethyl terephthalate as internal standard). The quantity of hesperidin was calculated by the ratio of the intensity of the compound to the known amount of internal standard. For HPLC analysis, the half gram of plant material was extracted with 60 ml of MeOH for 2 hours. The extracts were made 100 ml volume and analyzed by a Waters HPLC system using a YMC ODS column. The total flow rate was 1.0 ml/min with a sample volume 10 ${\mu}l$ and UV detection at 280nm. Results : The contents of hesperidin in Citrus unshiu was determined $5.33{\pm}0.06$% in the quantitative $^1H-NMR$ method and $5.15{\pm}0.12%$ in HPLC method. Using the quantitative $^1H-NMR$ the contents of hesperidin can be determined in much shorter time than the conventional HPLC measurements. Conclusions : From those results, the advantages of quantitative $^1H-NMR$ analysis are that can be analyzed to identify and quantify, and no reference compounds required for calibration curve. Besides, it allows rapid and simple quantification for hesperidin with an analysis time for only 10 min without any pre-purification steps.