• Korean Journal of Ichthyology
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• v.12 no.1
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• pp.14-19
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• 2000

The sequences of two fragments of 18s ribosomal DNA were determined to elucidate relationship between Pungitius sinensis and P. kaibarae. The proportion of G+C pair is 54.85% to 55.15% in P. kaibarae populations and 52.76% in P. sinensis. Number of substitutions ranges from 10~18 among the populations of P. kaibarae and up to 165 between P. sinensis and P. kaibarae. The value of sequence divergence were 0.0118~0.0195 among the populations of P. kaibarae and 0.2136~0.2306 between P. sinensis and P. kaibarae. The result of pairwise comparison of the sequences indicate that phylogenetic relationship between P. sinensis and P. kaibarae was differentiated to specific level.

• The Korean Journal of Zoology
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• v.33 no.3
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• pp.260-265
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• 1990

Pungitius sinensis specimens were collected from the Namdae River and Chusu River drained into Sea of Japan, and their morphological characters were investigated to determine if this species shows sexual dimorphism. The ratio of the head length to the body length revealed marked sexual difference; the head length in males was longer than in females. The snout length and body depth at anus showed a tendency to be larger in males than in females. This dimorphism seems to be related to breeding habits. The ethological significance of the larger head of males was discussed.

• Animal cells and systems
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• v.5 no.2
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• pp.127-132
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• 2001

The object of this study is to differentiate the reproductive behaviors of Pungitius sinensis and P. kaibarae inhabiting the southern part of the Korean Peninsula. We collected P. sinensis from Jusu stream, Okkyemyeon and P. kaibarae from Sacheon stream, Sacheon-myeon both in Gangwon province and subsequently raised and observed them in an aquarium. At the beginning of the reproductive season, male P. sinensis got tinged with dark green, made a territory, and built nests on the bottom. On the other hand, male P. kaibarae became black all over, its white ventral spines became conspicuous and built nests on the stems of waterweed off the bottom. In the courtship dance, male P. sinensis made frequent attempts to entice females into their nests after many bitings, while male P. kaibarae mostly did this with conspicuous jumpings. In courtship behaviors, the body's angle of male P. kaibarae with his head down was larger than that of male P. sinensis by 50-60 degrees. During courtship, the biting frequency as an index of aggressive behavior was greater in P. sinensis and the jumping frequency as an index of sexual behavior was greater in P. kaibarae. During the courtship dance, bitings tended to suppress jumpings, for P. sinensis, but not for P. kaibarae.

• Korean Journal of Ichthyology
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• v.28 no.3
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• pp.186-191
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• 2016

Distribution status and threatened assessment of endangered species, Pungitius sinensis (Pisces: Gasterosteidae), were investigated from March to October 2015 in Gangwon-do and Gyeongsangbuk-do etc., Korea. During the study period, a total of 854 individual P. sinensis were collected from 21 of 71 sampling stations using skimming nets. P. sinensis appeared in 23 streams during the past 30 years. However, it was only collected in 12 streams. Therefore, its habitats and populations were drastically reduced. The preferred habitat of P. sinensis was pool of the lower stream with lots of aquatic plants. Given these evidences of small occupying areas ($84km^2$) and drastic reduction in its habitats and populations, P. sinensis is considered as an endangered (EN) [B2ab(ii,iii,iv,v)] species based on IUCN Red List categories and criteria.

• The Korean Journal of Zoology
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• v.33 no.4
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• pp.499-508
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• 1990

Surveys of elecirophoretic variations in isozymes and proteins encoded by 25 persumtive loci were conducted to estimate the degree of genic variation and to assess species recognition for three species of the Family Gasterosteidae (Pisces) in Korea. The average degree of genic variation of Gasterosteus aculeatus was HD = 0.086, HG = 0.084, and P =36% and it was similar to Pungitius sinensis (HD = 0.080, HG = 0.091, P = 29%). But P.kaibarae was less variable than these species (HD = 0.034; HG = 0.034, P = 13%). The average genetic similarities among the populations in each species of Gasterosteus aculeatus pungitius sinensis and P. kaibarae were S = 0.963, S = 0.953 and S = 0.800 respectively.Yongchon and Kyongiu populations of P. kaibarae were genetically remote from the rest of the conspecific populations (S = 0.690). Chae (1988) also observed morphological differences between them. The value of genetic similariiy coefficients of S = 0.606 between P. sinensis and P. kaibarae is low enough to consider as specific level of genetic difference, but moderate rate of hybridization (44%) between them at a synpatric area of Kangnung indicates that isolating mechanism is incomplete. Therefore it is concluded that P. sinensis and P. kaffarae are semispecitic status. Intergeneric genetic similarities between the genus Gasrerosteus and Pungitius were S = 0.052 and this value is the lowest estimate up to date in freshwater fishes.

• Korean Journal of Ichthyology
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• v.2 no.1
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• pp.88-94
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• 1990

Natural hybrids between Pungitius sisensis sinensis and P. sinensis kaibarae were found in their sympatric area, a streamlet below Chibyon Reservoir in Kangnung city, Kangwondo, Korea. P. sinensis sinensis and P. sinensis kaibarae showed the same electropherograms as reported previously. However, about 10% of the specimens had peculiar banding patterns and they seemed to be hybrid between the two subspecies. They are divided further into two groups: sinensis-type and kaibarae-type hybrid. Two morphometric characters, snout length and depth of caudal peduncle, indicated the hybrid's property well. The color of testis of P. sinensis sinensis and P. sinensis kaibarae was grey and black, respectively, but that of hybrid was intermediate. The problem of reproductive isolation between the two groups raised by the finding of their hybrids was discussed.

• Korean Journal of Ichthyology
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• v.8 no.1
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• pp.74-82
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• 1996

The developmental process of scutes in the eightspine stickleback, Pungitius sinensis kaibarae (Tanaka), was investigated. The scutes appeared first on the caudal peduncle at 13mm and then on the thoracoabdominal part at 16mm. The scutes continued to develop and then the trachurus type of scute arrangement was completed at about 20mm. Finally the number of scutes was stabilized as 32 - 33 in the fish over 22mm. The developmental state of scutes was different according to part of body but most scutes except those of anteriormost part entered into late stage at about 25mm. The scutes of anteriormost part remained as scute of middle stage even though the fish became adults. All scutes were formed around neuromasts of lateral line. Few anterior plates(up to five plates) had additional neuromasts above the plates. But scale material was not accumulated around the additional neuromasts. Relationship between Korean and Japanese eightspine stickleback and the taxonomic meaning of the additional neuromasts were discussed.

• The Korean Journal of Ecology
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• v.22 no.3
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• pp.163-167
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• 1999

In order to investigate territorial behaviour, Pungitius sinensis kaibarae were collected from Sacheon-river, Kangwon-do, Korea. They are reared in aquarium with designed experimental region from March to May 1996. In this study, territorial behaviour was divided into 1) pre-territorial movements of a shoal under varying water-weeds position 2) individual ranking patterns in connection with total length of fish 3) the change in size of territorial maps during the parental stage. The movements of a shoal tend to prefer regions with water-weeds and the lower half of the aquarium. Competition for territory was fierce, and fish that are longer in total length dominate smaller one in occupying territory. Both males and females developed territorial behaviour as they grew. However, male's territory was enlarged according to the hatching and dispersion of fry. At this time, aggressive tendencies reached their peak.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.2
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• pp.262-266
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• 1996

The freshwater fish fauna were surveyed in the Namdae Stream, Kangreung Korea in August and October in 1992. The fish fauna includes 13species representing 5 family and 11 genera, The dominent species were Pungitius pungitius $(61.49\%)$ and Phoxinus lagowskii $(28.26\%)$ and the rare species wrere Hypomesus olidus, Pseudorasnora paws and Channa agus. Based on the previous studies there was a drastic changes not only in number of species but also in species composition.

• Korean Journal of Ecology and Environment
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• v.39 no.2 s.116
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• pp.265-270
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• 2006

The nucleotide sequence variations of mitochondrial DNA were investigated to understand genetic differentiation for five different Pungitius kaibarae populations at five study sites from selected four streams flowing into the East Sea. The complete sequences of mtDNA control regions of them were determined the constant sites 342 bp and variable sites 183 bp including parsimony infromative sites 122 bp. Based on the phylogenetic tree, five populations were monophyletic unit (97% MPand 100% NJ) and separated two groups (Myongpa-Songhuen stream group and others group). The population of Baebong stream (82nd bp, G-A) consistently formed them of the Jasan stream into a monophyletic unit even though it is closer then Myongpa stream (99th bp, T-C) from the Baebong stream. Further studies on the molecular phylogeny for the primary freshwater fish are needed to establish for the fish biodiversity conservation in mountainous and upland streams.