• Proceedings of the Botanical Society of Korea Conference
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• 1985.08b
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• pp.51-57
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• 1985

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1, 700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.

• Proceedings of the IEEK Conference
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• 2002.07a
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• pp.269-272
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• 2002

This work describes the use of neural networks (NNs) for interpreting voltammetric data, i.e., current-voltage spectra that obtained from the electrochemical reaction of analyte species at a gold electrode. Current-voltage spectra of glucose, fructose and ascorbic acid in mixtures obtained from dual-pulse staircase voltammetry (DPSV) was in the form of the mixed responses contain characteristics of the individual analytes approximately in proportion to their concentration. Extraction of individual analyte concentration from combined data was subsequently achieved using NNs. The combination of DPSV and NNs opens a possibility for simultaneous determination of mixtures of the species for fruit juices quality monitoring.

• Proceedings of the KIPE Conference
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• 2009.11a
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• pp.237-239
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• 2009

This paper presents a new developed 160kV-120kW Class MPC (magnetic pulse compressor) power supply for DeNOx, DeSOx system. The circuit consists of N-series connected CCPS (capacitor charging power supply) and MPC Tank. The MPC power supply developed compared to the conventional LC resonant type has many advantage, it was verified reliability of a product by module, simulator and tank connection test. Now, the developed MPC power supply is installed POSCO sintering plant for DeSOx, DeNOx system.

• Proceedings of the KIEE Conference
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• 2009.07a
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• pp.2019_2020
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• 2009

A recurrent surge oscillograph(RSO) test was performed at the Taean thermal power plant on #5 turbine generator. The test was conducted using a rotor reflectometer and digital oscilloscope. A DC voltage step is applied to each end of the rotor winding in turn. Each reflected wave, at the input end of the winding, is monitored and the two waveforms are superimposed automatically and monitored on a single channel oscilloscope. As the half windings in a rotor are identical, the two waveforms monitored at each end of the rotor will also be identical for a healthy winding. A winding with a fault will cause different voltages to be monitored at the two ends.

• Journal of Institute of Control, Robotics and Systems
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• v.17 no.2
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• pp.171-175
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• 2011

In this paper, I have developed a high-speed and high-resolution measuring device in order to check the performance of drycell. The system is developed for the drycell manufacturing plant. Measuring time is one of key factors to inference on the production speed. So the developed system is designed to generate the classified result up to 1200ea/min. In the other words, each product can be classified within 25ms. There have been many studies to estimate both state of charge as well as state of health, such as OCV (Open Circuit Voltage), SC (Short Circuit) and measuring impedance with frequency pulse. But those methods take a few second due to surface discharge. To overcome the phenomenon, I developed the method to engage the reverse current to two electrodes of battery. As a result, I could achieve to measure the indigenous capacity without the problem of surface discharge.

• Journal of the Korean Society for Nondestructive Testing
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• v.9 no.2
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• pp.42-49
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• 1989

It has not been performed to inspect the underclad cracking in Korea nuclear plant since there is no Code Requirements for inspection. However, underclad cracks in nuclear pressure vessels were reported firstly in 1970. The objection of this study is to be established the ultrasonic inspection techniques for underclad cracking. The ultrasonic inspection of bimetalic stainless steel weld is very difficult by high attenuation and multiple scattering at weld surface and weld/base metal interface. The various inspection methods using $70^{\circ}$ refracted longitudinal wave, 50/70 tandem transducer, $45^{\circ}\;and\;60^{\circ}$ single shear wave are compared. Experiments on limited specimens applied same condition to nuclear pressure vessels shows that $70^{\circ}$ refracted longitudinal wave method is the best one for the detection of underclad cracks. 50/70 tandem transducer using SPOT(Satellite Pulse Observation Technique) is more effective for underclad crack sizing than other sizing methods.

• Proceedings of the IEEK Conference
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• 2002.06e
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• pp.261-264
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• 2002

In this study, we used a 16-bit microprocessor, 80C196KC for a control part in order to develop a multi-functional wheel-chair system, and implemented a joy-stick to control this system. For the complete system, we used a commercial electromotive wheelchair as a basic plant, and applied an encoder to get the rotating number of the motor to transfer data to the MCU to control the motor. We used PWM (Pulse Width Modulation) method to control the wheel-chair motor where a H-bridge circuit was configured. We used the fuzzy control algorithm for the operation of DC motor, which was attached to the electromotive wheelchair and manipulated following the change of the joystick position while a user was controlling the joystick. He also could control the speed and direction of DC motor as well as control position information.

• Proceedings of the KIEE Conference
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• 1997.11a
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• pp.77-79
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• 1997

This paper describes a nonlinear state observer to identify the rotor position and velocity of a hybrid-type linear pulse motor(LPM). Since the observer's error dynamics of a hybrid-type LPM is highly nonlinear, the stability of the proposed observer is analyzed using an approximated error model. The optimal choices of the proposed observer gains are also discussed. Our extensive simulation study shows that the proposed observer can identify rotor position and velocity and is robust with respect to uncertainties in mechanical parameters and load force.

• Proceedings of the KWS Conference
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• 2003.05a
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• pp.155-158
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• 2003

Orbital GTA welding is used in the pressure pipe line of auto-mobile, LNG and plant piping. To weld the pipe line safely, to some extent, the back bead must be formed in the root pass. In the plate welding the back bead can be observed, but in the pipe welding, the back bead can not be observed directly. In the welding around circumference, the parameters such as gravity, surface tension and arc force are different at each position. And then D/B for welding condition at each position are required. We also studied about the setting of the optimum orbital welding condition by controlling heat input.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.24 no.9
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• pp.79-84
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• 2010

This paper discusses the control of generator field using dual IGBT PWM regeneration method to target brushless synchronous generator. If one of PWM bridges happens to fault, it transfers automatically and can be in charge of full load. Also it has an advantage of the operation which UPS connected in parallel with PWM bridge can supply power to excitation system in condition of main power loss. This PWM system supplies field current to generator in one quadrature operation, regenerates field coil energy to main power supplier in four quadrature operation. We designed, manufactured and applied the first trial product at J-power plant.