• Journal of Plant Biology
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• v.34 no.1
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• pp.37-43
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• 1991

Mesophyll protoplasts of Nicoliana labacum ($NR^{-}/SR^{+}$) and N glulinosa were electrofused with AC field of 0.5 MHz and 1 kV DC pulse for 2 ms. Fused protoplasts were selected and cultured to the green cell clusters in $MSNO_3$ medium containing 1.2 mg!ml streptomycin sulfate. Four plant lines regenerated from selected colonies showed both parental morphological characteristics of leaf and flower and these plant lines were confirmed as somatic hybrids based on electrophoretic patterns of leaf peroxidase. In XhoI restriction patterns of chloroplast DNA, these hybrid plant lines expressed both parent common restriction sites and parent specific sites. One of these hybrid lines exhibited interspecific pattern of both parental chloroplast genomes. indicating nine both parent common sites, one N labacum specific site and two N glutinosa specific sites. sites.

• Journal of the Korean Society for Nondestructive Testing
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• v.30 no.4
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• pp.317-323
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• 2010

The manual ultrasonic examination for the nuclear power plant piping welds has been demonstrated by using KPD(Korean Performance Demonstration) generic procedure. For automated ultrasonic examination, there is no generic procedure and it should be qualified by using applicable automated equipment. Until now, most of qualified procedures used pulse-echo technique and there is no qualified procedure using phased array technique. In this study, data acquisition and analysis software were developed and phased-array transducer and wedge were designed to implement phased array technique for nuclear power plant in-service inspection. The developed procedure are qualified for performance demonstration for the flaw detection, length sizing and depth sizing. The qualified procedure will be applied for the field examination in the nuclear power plant piping weld inspection.

• Journal of Advanced Navigation Technology
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• v.23 no.6
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• pp.554-560
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• 2019

Excore Nuclear Flux Monitoring System in Nuclear Power Plant monitors continuous reactor power up to maximum 200%. The monitoring method, however, has to be different depending on the reactor power level. Because the logarithmic pulse signals must be counted and processed exactly due to large uncertainty if their levels are low, on the other hand, they must be processed through statistical methodolgies if theirs are high to get exact monitoring values, in point of thermal degradation view. Therefore, we developed thermal degradation input signal selection circuit to transfer low level reactor power monitoring circuit to high level reactor power circuit at rated value in this paper. We proved their validities through testing them using real data used in nuclear power plant and analyzed their results. And, These methods will be used to measure the neutron level of excore nuclear flux monitoring system in nuclear power plant.

• Korean Journal of Environmental Agriculture
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• v.32 no.2
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• pp.123-127
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• 2013

BACKGROUND: The incandescent bulb and compact fluorescent lamp are widely using as a light sources for daylength extension of chrysanthemum. But, these light sources consume a lot of electricity and have short longevity. A light-emitting diode (LED) is a semi conductor light source. LEDs have many advantages over incandescent light sources including lower energy consumption, longer lifetime. In this study, we investigated the intensity of red light to control flowering of chrysanthemum (Dendranthema grandiflorum cv. "Shinma") by using LEDs. METHODS AND RESULTS: The red (660 nm) and far-red (730 nm) light were irradiated subsequently to investigate photo-reversible flowering responses of chrysanthemum. The flowering of chrysanthemum was inhibited by night interruption with red light but subsequently irradiated far-red light induced the flowering of chrysanthemum. This photoreversibility, reversion of the inductive effect of a brief red light pulse by a subsequent far-red light pulse, is a property of photo responses regulated by the plant photoreceptor phytochrome B. Four different intensity of red light of 0.7, 1.4, 2.1, and $2.8{\mu}mol/m^2/s$ (PAR) were irradiated at growth room in order to determine the threshold for floral inhibition of chrysanthemum. Over $1.4{\mu}mol/m^2/s$ of the red lights irradiated chrysanthemums were not flowered. The plant length, fresh weight, number of leaves, and leaf area of chrysanthemum irradiated with red light were increased by 17%, 36%, 11%, and 48%, respectively, compared to those of compact fluorescent lamp. CONCLUSION(S): The red light and subsequential far-red light showed that the photoreversibility on flowering of chrysanthemum. The red light ($1.4{\mu}mol/m^2/s$ of red LEDs) and white light (50 Lux of compact fluorescent lamp) have the same effect on inhibition of flowering in chrysanthemum. Additionally, the red light increased the plant height and dry weight of chrysanthemum.

• Korean Journal of Environmental Agriculture
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• v.39 no.4
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• pp.312-318
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• 2020

BACKGROUND: Photoreversibility, a reversion of the inductive effect of a brief red light pulse by a subsequent far red light pulse, is a property of photo responses regulated by the plant photoreceptor phytochrome B. Plants use photoreceptors to sense photo signal and to adapt and modify their morphological and physiological properties. Phytochrome recognizes red light and far red light and plays an important role in regulating plant growth and development. METHODS AND RESULTS: The reversal responses of growth and fruiting characteristics were investigated to increase the yield of oriental melon (Cucumis Melo L. var. Kumsargakieuncheon) by means of controlling light quality in a plastic house. Red (R:660nm) and far red (FR:730nm) lights were subsequently irradiated on the whole stems and leaves of the oriental melon plant during growing periods, using red and far red LEDs as light sources, from 9:00 PM daily for 15 minutes. The intensities of R and FR light were 0.322-0.430 μmol m-2s-1 and 0.250-0.366 μmol m-2s-1, respectively. Compared to R light irradiation, combination of R and FR light irradiation increased the length of internode, number of axillary stems, number of female flowers, and fruit number of oriental melons. The results of treatment with R were similar to R-FR-R light irradiation in terms of length of internode, number of axillary stems, number of female flowers, and number of fruits. When FR treatment was considered, R-FR and R-FR-R-FR light irradiation had similarities in responses. These reversal responses revealed that oriental melon showed a photoreversibility of growth characteristics, flowering, and fruiting. CONCLUSION: These results suggested the possibility of phytochrome regulation of female flower formation and fruiting in oriental melon. The fruit weight of the oriental melon was the heaviest with the R light irradiation, while the number of fruits was the highest with the FR light. With the FR light irradiation, the fruit weight was not significantly higher compared to that of the control. Meanwhile, the yield of oriental melon fruits increased by 28-36% according to the intensities of the FR light due to the increases of the number of fruits.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2011.04a
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• pp.648-654
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• 2011

The feed-water piping system constitutes a complex flow impedance network incorporating dynamic transfer characteristics which will amplify some pulsation frequencies. Understanding pressure pulsation waves for the feed-water recirculation piping system with cavitation problem of flow control valve is very important to prevent acoustic resonance. Feed water recirculation piping system is excited by potential sources of the shock pulse waves by cavitation of flow control valve. The pulsation becomes the source of structural vibration at the piping system. If it coincides with the natural frequency of the pipe system, excessive vibration results. High-level vibration due to the pressure pulsation affects the reliability of the plant piping system. This paper discusses the piping vibration due to the effect of shock pulsation by the cavitation of the flow control valves for the recirculation piping of feed-water pump system in combined cycle power plants.

• Nuclear Engineering and Technology
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• v.53 no.6
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• pp.1858-1868
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• 2021

Resisting an accidental impact of large commercial aircrafts is an important aspect of advanced nuclear power plant (NPP) design. Especially after the 9·11 event, some regulations were enacted, which required the design of NPPs should consider the accidental impact of large commercial aircrafts. Normal working of equipment is important for stopping reactor under an impact when an NPP is in operation. However, there is a lack of reliable analysis and research on the impact test of nuclear prototype equipment. Therefore, in order to study the response of the equipment under high acceleration impact, a centrifugal pump is selected as the research object to perform the impact test. A horizontal half-sinusoidal pulse wave was applied to the working pump. The test results show that the horizontal response of the motor and flange is greater compared to other parts, as well as the vertical response of the coupling. The stress response of the pump body support and motor support is high, hence these parts should be considered in the design of the pump. Finally, combined with the damage and stress evaluation results of the pump under different amplitudes, the ultimate impact acceleration that the pump can withstand is given.

• Journal of Animal Science and Technology
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• v.65 no.1
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• pp.32-56
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• 2023

This review explores the factors that improve meat protein digestibility and applies the findings to the development of home meal replacements with improved protein digestion rates in older adults. Various methods improve the digestion rate of proteins, such as heat, ultrasound, high pressure, or pulse electric field. In addition, probiotics aid in protein digestion by improving the function of digestive organs and secreting enzymes. Plant-derived proteases, such as papain, bromelain, ficin, actinidin, or zingibain, can also improve the protein digestion rate; however, the digestion rate is dependent on the plant enzyme used and protein characteristics. Sous vide processing improves the rate and extent of protein digestibility, but the protein digestion rate decreases with increasing temperature and heating time. Ultrasound, high pressure, or pulsed electric field treatments degrade the protein structure and increase the proteolytic enzyme contact area to improve the protein digestion rate.

• Journal of the Korean Society for Nondestructive Testing
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• v.26 no.1
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• pp.12-17
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• 2006

A steam generator of nuclear power plant has thousands of thin tubes. These tubes play an important role in maintaining the pressure boundary between the primary and secondary side of nuclear power plant. The steam generator tube is easy to be damaged because of the severe operating conditions such as the high temperature and pressure. Therefore, tremendous efforts are made to assess the structural integrity of the steam generator tubes. The eddy current test is the most popular non-destructive test to assess the integrity of the tubes. However, the eddy current test has the limitation to size the flaw accurately because the eddy current signal behavior depends on the total volume of flaw. This paper shows the possibility that the ultrasonic test method can be applied to detect the flaws in the steam generator tubes and to measure them quantitatively. From the test results, it is expected that if the ultrasonic test is put to practical use in the steam generator tube inspection, the inspection results will be improved.

• Proceedings of the Botanical Society of Korea Conference
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• 1999.07a
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• pp.11-15
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• 1999

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1,700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.