• Journal of Ginseng Research
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• v.45 no.6
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• pp.734-743
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• 2021

Background: The underlying mechanisms of the potential tumor-suppressive effects of ginsenoside Rh2 are complex. N6-methyladenosine (m6A) RNA methylation is usually dysregulated in cancer. This study explored the regulatory effect of ginsenoside Rh2 on m6A RNA methylation in cancer. Methods: m6A RNA quantification and gene-specific m6A RIP-qPCR assays were applied to assess total and gene-specific m6A RNA levels. Co-immunoprecipitation, fractionation western blotting, and immunofluorescence staining were performed to detect protein interactions and distribution. QRT-PCR, dual-luciferase, and ChIP-qPCR assays were conducted to check the transcriptional regulation. Results: Ginsenoside Rh2 reduces m6A RNA methylation and KIF26B expression in a dose-dependent manner in some cancers. KIF26B interacts with ZC3H13 and CBLL1 in the cytoplasm of cancer cells and enhances their nuclear distribution. KIF26B inhibition reduces m6A RNA methylation level in cancer cells. SRF bound to the KIF26B promoter and activated its transcription. SRF mRNA m6A abundance significantly decreased upon KIF26B silencing. SRF knockdown suppressed cancer cell proliferation and growth both in vitro and in vivo, the effect of which was partly rescued by KIF26B overexpression. Conclusion: ginsenoside Rh2 reduces m6A RNA methylation via downregulating KIF26B expression in some cancer cells. KIF26B elevates m6A RNA methylation via enhancing ZC3H13/CBLL1 nuclear localization. KIF26B-SRF forms a positive feedback loop facilitating tumor growth.

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.11a
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• pp.27.2-27.2
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• 2006

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.3
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• pp.161-170
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• 1975

Status of agriculture in Jeju province were investigated to find out problems and solutions in view of weather, soils and crop productions. Two hundred harmful windy days per year force to build strong wind-break forest near to farm. Somewhat severe drought situation in croping could be completely avoided by good irrigation system using abundant underground water resources. Poor fertility of soils should be improved by using much compost, lime, fused phosphate, and high dose fertilizers. Optimum fertilizer level for each crop should be determined with special consideration on characteristics of volcanic ash soil.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.1
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• pp.61-66
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• 1993

Sixteen Korean native bulls weighing about 211 kg were randomly allotted to investigate the effect of different dietary calcium levels on performance. Feeding period was divided into growing period and fattening period for 180 days. Four calcium levels (0.44, 0.58, 0.72 and 0.86%) were used to each period. Bulls fed the diet containing 0.72% calcium had greater body weight gain and consumed more feed than bulls fed diets containing 0.44, 0.58 and 0.86% calcium and improved in feed efficiency. Bulls fed the diet containing 0.44% calcium were worst in performance. It can be concluded that Korean native bulls in growing and fattening period required no more than 0.72% calcium in diet that contained 0.55% phosphorus, and appeared that when ratio of calcium to phosphorus intake was 1.45, performance of Korean native bulls was most beneficial.

• Journal of Korean Society of Rural Planning
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• v.13 no.4
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• pp.53-67
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• 2007

This study analyzed the probability that experiment stations, agricultural technology and extension centers, provincial agricultural research and extension services, central government organs, or civilian and other related organs will be the first choice of the compositional subjects of local innovation networks. While gender effect was statistically insignificant, educational level, income, main acquired information, sources of necessary information, and frequency of information acquisition sessions were significant, and the preference ranking model was highly relevant. According to the analysis, highly academic and business-related information was most likely to be acquired from the civilian sector; agricultural technology such as technology, crops/plants, storage, and circulation was most likely to be acquired from experiment stations and provincial agricultural research and extension services; and information on agricultural production was most likely to be acquired from agricultural technology centers.

• Korean Journal of Environment and Ecology
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• v.36 no.3
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• pp.318-326
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• 2022

The Convention on Biodiversity (CBD) recommends that 17% of the land be designated as a protected area to counter global environmental problems. Korea also realized a need to designate protected areas according to the international level and explain the significance of designating protected areas. Accordingly, studies on ecosystem services are required. In Korea, the protected areas are designated as national parks, provincial parks, and county parks by hierarchy under the Natural Parks Act. However, as priority was on political and administrative aspects, research on ecosystem service value evaluation and habitat management were concentrated in national parks, and provincial and county parks were relatively neglected. Therefore, more studies on provincial and county parks are necessary. In this study, habitat quality for Gajisan Provincial Park, where there were few studies on habitat management and ecosystem service valuation, was evaluated using the InVEST Habitat Quality model among the InVEST models. The analysis results were compared with 16 mountainous national parks. The results showed that the habitat quality value of Gajisan Provincial Park was 0.83, higher than that of the surrounding areas. The analysis of habitat quality in three districts showed 0,84 for the Tongdosa and Naewonsa districts and 0.83 for the Seoknamsa district. By use district, the nature conservation district, the natural environment district, the cultural heritage district, and the park village district had the highest habitat quality value in that order. Compared with the existing habitat quality analysis results of national parks, Gajisan Provincial Park showed naturalness at the level of Mudeungsan National Park. These results can be used as objective data for establishing policies and management plans to preserve biodiversity and promote ecosystem services in provincial parks.

• Molecules and Cells
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• v.42 no.12
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• pp.906-918
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• 2019

MicroRNA-223-3p (miR-223-3p) is one of the potential microRNAs that have been shown to alleviate inflammatory responses in pre-clinical investigations and is highly encased in exosomes derived from bone mesenchymal stem cells (MSC-exosomes). MSC-exosomes are able to function as carriers to deliver microRNAs into cells. Autoimmune hepatitis is one of the challenging liver diseases with no effective treatment other than steroid hormones. Here, we examined whether MSC-exosomes can transfer miR-223-3p to treat autoimmune hepatitis in an experimental model. We found that MSC-exosomes were successfully incorporated with miR-223-3p and delivered miR-223-3p into macrophages. Moreover, there was no toxic effect of exosomes on the macrophages. Furthermore, treatments of either exosomes or exosomes with miR-223-3p successfully attenuated inflammatory responses in the liver of autoimmune hepatitis and inflammatory cytokine release in both the liver and macrophages. The mechanism may be related to the regulation of miR-223-3p level and STAT3 expression in the liver and macrophages. These results suggest that MSC-exosomes can be used to deliver miR-223-3p for the treatment of autoimmune hepatitis.

• Geomechanics and Engineering
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• v.36 no.6
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• pp.563-573
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• 2024

In this study, various countermeasures used to mitigate tunnel deformations due to nearby multi-propped basement excavation in soft clay are explored by three-dimensional numerical analyses. Field measurements are used to calibrate the numerical model and model parameters. Since concrete slabs can constrain soil and retaining wall movements, tunnel movements reach the maximum value when soils are excavated to the formation level of basement. Deformation shapes of an existing tunnel due to adjacent basement excavation are greatly affected by relative position between tunnel and basement. When the tunnel is located above or far below the formation level of basement, it elongates downward-toward or upward-toward the basement, respectively. It is found that tunnel movements concentrate in a triangular zone with a width of 2 H_e (i.e., final excavation depth) and a depth of 1 D (i.e., tunnel diameter) above or 1 D below the formation level of basement. By increasing retaining wall thickness from 0.4 m to 0.9 m, tunnel movements decrease by up to 56.7%. Moreover, tunnel movements are reduced by up to 80.7% and 61.3%, respectively, when the entire depth and width of soil within basement are reinforced. Installation of isolation wall can greatly reduce tunnel movements due to adjacent basement excavation, especially for tunnel with a shallow burial depth. The effectiveness of isolation wall to reduce tunnel movement is negligible unless the wall reaches the level of tunnel invert.

• Korean Journal of Environmental Agriculture
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• v.29 no.3
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• pp.282-286
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• 2010

The present studies were carried out for three years from 2007 until 2009 to control nematode and to increase yield of cucumber by crashed-rape seed application at polyethylene film house. Crashed-rape seed has a lot of glucosinolate. Myrosinase decompose gulcosinolate into isothiocyanate and thiocyanate when crashedrape seed go to decay at soil. Those chemical compounds act on poison to nematode at soil. When the crashedrape seed treated at soil, an amount of thiocyanate at soil was risen up. Thiocyanate of plot treated with 200 and 400 kg crashed-rape seed per 10a was 30 and 40 mg/kg, respectively. Nematode(meloidogyne spp) population at soil was 13 to 17 nematodes per dried soil 300g. Yield of cucumber increased 6 to 15 percent to be compared with control. While, Nematode(meloidogyne spp) population of control plot were 463 nematodes per dried soil 300 g. This level was much higher than 150 nematodes which can be brought about injury to plant. Even if the more an amount of crashed-rape seed application, the higher yield of cucumber and control effect of nematode. Consider economical efficiency, 200 kg of crashed-rape seed per 10a was the most effective. Therefore, we suggest applying 200 kg of crashed-rape seed per 10a to control soil nematode when culture cucumber at plastic film house.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.8
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• pp.1265-1272
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• 2020

Objective: To evaluate the effect of feeding acidified milk on the growth and fecal microbial diversity of dairy calves. Methods: Twenty healthy 3-day-old female Holstein calves with similar body weights were selected and randomly divided into two groups. One group was fed pasteurized milk (PM, Control), while the other was fed acidified milk (AM) ad libitum until weaned (day 60). The experiment lasted until day 180. Results: There was no difference in the nutritional components between PM and AM. The numbers of Escherichia coli and total bacteria in AM were lower than in PM. At 31 to 40 and 41 to 50 days of age, the milk intake of calves fed AM was higher than that of calves fed PM (p<0.05), and the solid feed intake of calves fed AM was higher than that of calves fed PM at 61 to 90 days (p<0.05). The average daily gain of calves fed AM was also higher than that of calves fed PM at 31 to 60, 61 to 180, and 7 to 180 days (p<0.05). The calves fed AM tended to have a lower diarrhea rate than those fed PM (p = 0.059). Bacteroides had the highest abundance in the feces of calves fed AM on day 50, while Ruminococcaceae_UCG_005 had the highest abundance in the feces of calves fed AM on day 90 and calves fed PM on days 50 and 90. At the taxonomic level, the linear discriminant analysis scores of 27 microorganisms in the feces of calves fed AM and PM on days 50 and 90 were higher than 4.0. Conclusion: Feeding AM increased calf average daily gain and affected fecal bacterial diversity.