• Title/Summary/Keyword: Protein quality

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Evaluation of antioxidant property of heat shock protein 90 from duck muscle

  • Zhang, Muhan;Wang, Daoying;Xu, Xinglian;Xu, Weimin
    • Animal Bioscience
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    • v.34 no.4
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    • pp.724-733
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    • 2021
  • Objective: The objectives of this study were to investigate the direct antioxidative effect of 90 Kda heat shock protein (Hsp90) obtained from duck muscle. Methods: The interaction of Hsp90 with phospholipids and oxidized phospholipids was studied with surface plasmon resonance (SPR), and their further oxidation in the presence of Hsp90 was evaluated with thiobarbituric acid reactive substances (TBARS) assay. The scavenging effect on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS) was measured, and the electron paramagnetic resonance (EPR) spectroscopy in combination with 5-tert-Butoxycarbonyl-5-methyl-1-pyrroline-N-oxide and 2-phenyl-4,4,5,5,-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) was utilized to determine the abilities of Hsp90 in scavenging hydroxyl and PTIO radicals. Results: SPR showed Hsp90 could bind with both phospholipids and oxidized phospholipids, and prevent their further oxidation by the TBARS assay. The DPPH and ABTS scavenging activity increased with Hsp90 concentration, and could reach 27% and 20% respectively at the protein concentration of 50 μM. The EPR spectra demonstrated Hsp90 could directly scavenge ·OH and PTIO· radicals. Conclusion: This suggests that Hsp90, a natural antioxidant in meat, may play an important role in cellular defense against oxidative stress, and may have potential use in meat products.

Characteristics of high β-glucan resources quality of Barley

  • Seul-Gi Park;Young-Mi Yoon;Jin-Cheon Park;Chang-Hyun Lee;Tae-Il Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.308-308
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    • 2022
  • β-glucan, a nonstarch polysaccharide, is one of the main functional component in the cell wall of barley. This study was quality characteristics to use a korean variety with a high β-glucan as an original material for developing functional food. The high β-glucan 'Jeonju528, and 'Betaone' were compared with 'Hyeyang', 'Dahyang', 'Hwanggeumchal' and 'Glacier AC38' total 6 varieties. Seed section dyed to classify of Waxy/non-waxy type, starch granule was tested and moisture, protein, amylose, and β-glucan of whole grains and pearl barleys were experiment. Whole grains were the average protein of 13.2% and were the average starch 50.1%. β-glucan of whole grains were 5.3-10.0%, and amylose were 3.0-23.4%. Pearl barleys were the average protein of 11.7% and were the average starch of 65.0%. β-glucan of pearl barleys were 6.5-12.3%, and amylose were 3.6-31.1%. As a results of the correlation analysis were recognized significance among varieties for protein, starch and β-glucan but there was no difference in other traits. It was concluded that amylose showed a positive correlation with starch and β-glucan showed a negative correlation with amylose.

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Studies on Rice Quality of Chucheongbyeo Cultivated on Gyeonggi and Other Province (경기지역과 다른 지역에서 생산된 추청벼의 특성에 관한 연구)

  • 이병영;손종록
    • The Korean Journal of Food And Nutrition
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    • v.8 no.4
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    • pp.301-306
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    • 1995
  • This study was carried out to find out rice quality of Chucheongbyeo cultivated on Gyeonggj and other province. Rice type, color difference, amylograph of milled rice and texturograph of cooked rice were not significant between two cultivated region. And fat, protein, Mg and Ca in milled rice were not significant but p, K and Na were significantly different at l% the level between them as 2, 210~2, 420ppwt 1, 304~1, 538ppm and 212~236ppm in Gyeonggi province cultivated rice, and 2, 130~2, 180ppm, 1, 133~1, 180ppm and 197~217ppm in other province, respectively. Also amylose content, alkali distribution and gel consistency were not significant between them.

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Genetic Ana1ysis for Rice Grain Properties Using a Doubled Haploid Population

  • Qin, Yang;Kim, Suk-Man;Sohn, Jae-Keun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.2
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    • pp.123-128
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    • 2007
  • Demand for high quality rice has always been a major factor in the international rice marketing. In the present study, doubled haploid (DH) population derived from anther culture of a Tongil/japonica hybrid was used for genetic analysis of rice grain quality. The average values of DH lines for grain weight, grain length and the ratio of grain length to width were near the mid-parent value. More than 40% DH lines showed transgressive segregation for grain weight, length, amylose and lipid content, but less than 10% DH lines observed on ratio of length to width and grain thickness were transgressive segregation. Correlation analysis between appearance qualities and physicochemical characters indicated that grain width and grain thickness both significantly and negatively correlated to protein and lipid content. A highly significant negative correlation between protein content and amylose content was observed.

Quality Characteristics of Beef Patty Containing Gums (Gum류를 첨가한 우육 Patty의 품질 특성)

  • 정인철;김도완;이경수
    • Journal of the East Asian Society of Dietary Life
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    • v.10 no.5
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    • pp.403-410
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    • 2000
  • In order to investigate the possibility of lwo fat meat products, beef patties were prepared with gums such as carrageenan, methyl cellulose, and xanthangum. The quality characteristics of beef patties were examined. Moisture contents of raw and cooked patties of control were lower than those of patties containing gums, and fat content was higher than those of containing gums. There was no significant difference in the protein contents of patties. In case of L-value(lightness) of raw patty, the control was higher than those patties containing gums. a-(redness) and b-value(yellowness) among patties were not significantly different. Cooking yield, fat retention, and water holding capacity of beef patties containing gums were higher than control beef patty, but salt soluble protein and gel strength were not significantly different. Significant difference did not exist among beef patties in hardness, but the chewiness and gumminess of patties containing gums were higher than control. In case of sensory evaluation, there was no significant difference among patties in aroma and palatability. Texture of control was higher than patties containing gums, and juiciness of control was higher than patties containing carrageenan.

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Change of Physicochemical Quality According to Its Storage Temperature in Garlic (Allium sativum L.) (마늘의 저장온도에 따른 이화학적 품질변화)

  • 장현세;홍경훈
    • Food Science and Preservation
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    • v.5 no.2
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    • pp.119-122
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    • 1998
  • This study was conducted to blow the effect of postharvest physiological changes on garlic quality according to its ecotypes and storage temperatures. The changes of water, total soluble solids, crude stein, md total fructans were measured and the rates of respiration and sprouting were analyzed during storage at 20$^{\circ}C$ and 30$^{\circ}C$. The decrease of water content and the increase of total soluble solids were reversely appeared during garlic storage. The crude protein content was gradually increased during storage but total fructan content was decrased. The respiration late was maximized at 60days after storage and the spouting rate was gradually increased. In the aspect of ecotypic characteristics, the water content, fructan content and sprouting rate were higher in 'Namdo' cultivar than those of southern type. The high storage temperature (30$^{\circ}C$) controlled spouting and loss of fructan, and it was effective to maintain the garlic quality.

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Quality Characteristics of Soy Ice Creams as Affected by Enzyme Hydrolysis Times and Added Calciums (효소처리시간과 칼슘의 종류를 달리한 칼슘강화 콩아이스크림의 품질특성)

  • 김지영;이숙영
    • Korean journal of food and cookery science
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    • v.19 no.2
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    • pp.216-222
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    • 2003
  • The effects of hydrolysis times and calcium source additions (calcium lactate, calcium carbonate), on the qualify characteristics of soy ice cream prepared with soy protein isolate(SPI), were studied. Increasing the hydrolysis time decreased the viscosity and overrun of soy ice creams, but increased the melt-down property. The addition of calcium lactate increased the viscosity of the soy ice cream mix, but no changes were observed from the calcium carbonate addition. The overrun of calcium lactate samples was higher than on addition of calcium carbonate. The addition of calcium lactate and calcium carbonate resulted in decreased melt-down properties, although these effects were more evident in the calcium lactate samples. However, calcium carbonate addition resulted in higher scores in the overall quality of the soy ice creams. In conclusion, better soy ice cream could be prepared by treating the SPI with Flavorzyme for 50 min, along with calcium fortification in the form of calcium carbonate.

Effects of a Novel p.A41P Mutation in the Swine Myogenic factor 5 (MYF5) Gene on Protein Stabilizing, Muscle Fiber Characteristics and Meat Quality

  • Ryu, Youn-Chul;Lee, Eun-A;Chai, Han-Ha;Park, Jong-Eun;Kim, Jun-Mo
    • Food Science of Animal Resources
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    • v.38 no.4
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    • pp.711-717
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    • 2018
  • Myogenic factor 5 (MYF5) plays an important role in regulating skeletal muscle fiber characteristics, consequently affecting meat production and quality. We identified a novel p.A41P mutation in exon1 of the porcine MYF5 gene by direct sequencing. The mutation was predicted to be destabilizing in protein structure based on the resultant amino acid substitution. We estimated the significant substitution effect of p.A41P on the energy stabilization of Myf5 protein structure. Then, we demonstrated that the mutation in Yorkshire population significantly affected muscle fiber type I composition (p<0.05), loin-eye area of lean meat content (p<0.05) and filter-fluid uptake of meat quality (p<0.01). Furthermore, dominant effects significantly influenced total muscle fiber number (p<0.05). This study suggests that the novel p.A41P mutation in porcine MYF5 may be a valuable genetic marker to affect the muscle fiber characteristics and consequently improve meat production quality and quantity.

Analysis of Paddy Soil Chemical Properties and Rice Quality in Central Area (Sejong) in Korea

  • Choi, Nag-Gor;Park, Jong-Hyun;Kang, Seong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.51 no.1
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    • pp.61-70
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    • 2018
  • This study was carried out to review and compare crop cultivations upon chemical properties of paddy soil and qualitative characteristics of rice in Sejong-city from a point of view of farming extension to rice farmers and to utilize the result of the study as a basic guideline for precise agricultural practice. The pH in soils of Sejong was about 6.1-6.6 and had no difference with an average pH of paddy soils in Chung-Nam with pH 6.1. However, the average of organic matter, calcium (Ca) and available silicate in Sejong was lower than the average of them in Chung-Nam. The yields of rice were higher in 2010 than in 2011 and 2012, and the protein contents of rice were the highest in 2011 while the lowest water contents of rice in 2011. The protein contents upon regions were the highest in 2011 with 6.1%, and the amylose contents were the highest in Yeondong-myun, Jeoneu-myun, and Yeonseo-myun in 2010 while Kumnam-myun and Jeondong-myun were the highest in 2012. With the increase of precipitation, the protein content level in rice was increased while the amylose content level tended to decrease. Correlations between the chemical properties of paddy soil and the quality of rice and between level of organic matter in soil and amylose contents were negative (r = -0.507), and the correlation between the moisture contents and amylose contents (r = 0.419) and between the water contents and whiteness (r = 0.485) were positive. Because the quantity and quality of rice yield is determined by the soil characteristics, the consultation to farmers for the proactive soil analysis and for the maintenance of stable level of pH, organic matter and available silicate based on historical results of analysis is highly recommended. Also, the analysis on the effect of the weather and the soil characteristics affecting the quality and quantity of rice would be another good way.

Effect of Planting Date and Plant Density on Yield and Quality of Soybean Forage in Jeju

  • Kang, Young-Kil;Kim, Hyun-Tae;Cho, Nam-Ki;Kim, Yeong-Chan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.2
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    • pp.95-99
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    • 2001
  • Soybean [Glycine max (L.) Merr.) is known to produce the highest total digestible mutrients (TDN) yield among summer grain legumes in Jeju area but little is known about the effects of cultural practices on forage yield and quality. A determinate soybean cv. Baegunkong was planted on 5 June, 20 June, and 3 July and grown at four plant densities (30, 50, 70 and 90 plants $m^{-2}$ in 1998 in Jeju to evaluate the effects of planting date and plant density on the yield and quality of soybean forage. Days to flowering decreased from 47 to 38 days, average plant height from 61 to 51cm and main stem diameter from 6.31 to 5.00mm as planting was delayed from 5 June to 3 July. Average plant height quadratically increased from 45 to 62cm as plant density increased from 30 to 90 plants $m^{-2}$. Planting date did not affect the average dry matter, crude protein, and TDN yields. The average dry matter and TDN yields displayed a quadratic response to plant density and the optimum plant density for both dry matter and TDN yields was estimated about 60 plants $m^{-2}$. Plant density had no effect on crude protein yield. Planting date did not significantly influence forage quality. The crude protein content was not significantly influenced by plant density. Increasing plant density slightly increased acid detergent fiber content but slightly decreased TDN content.

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