Genetic Ana1ysis for Rice Grain Properties Using a Doubled Haploid Population

  • Qin, Yang (Department of Agronomy, Kyungpook National University) ;
  • Kim, Suk-Man (Department of Agronomy, Kyungpook National University) ;
  • Sohn, Jae-Keun (Department of Agronomy, Kyungpook National University)
  • Published : 2007.06.30

Abstract

Demand for high quality rice has always been a major factor in the international rice marketing. In the present study, doubled haploid (DH) population derived from anther culture of a Tongil/japonica hybrid was used for genetic analysis of rice grain quality. The average values of DH lines for grain weight, grain length and the ratio of grain length to width were near the mid-parent value. More than 40% DH lines showed transgressive segregation for grain weight, length, amylose and lipid content, but less than 10% DH lines observed on ratio of length to width and grain thickness were transgressive segregation. Correlation analysis between appearance qualities and physicochemical characters indicated that grain width and grain thickness both significantly and negatively correlated to protein and lipid content. A highly significant negative correlation between protein content and amylose content was observed.

Keywords

References

  1. Aluko, G., C. Martinez, J. Tohme, C. Castano, C. Bergman, and J. H. Oard, 2004. QTL mapping of grain quality traits from the interspecific cross Oryza sativa${\times}O$. glaberrima. Theor. Appl. Genet. 109 : 630-639
  2. Choi, H. C., H. C. Hong, Y. G. Kim, and B. H. Nahm, 1999. Varietal variations in physicochemical characteristics and amylopectin structure of grain in glutinous rice. Korean J. Crop Sci. 44(3) : 207-213
  3. Fan, C. C., X. Q. Yu, Y. Z. Xing, C. G. Xu, L. J. Luo, and Q. Zhang, 2005. The main effects, epistatic effects and environmental interactions of QTLs on the cooking and eating quality of rice in a doubled-haploid line population. Theor. Appl. Genet. 110 : 1445-1452 https://doi.org/10.1007/s00122-005-1975-y
  4. He, P., S. G. Li, Q. Qian, Y. Q. Ma, J. Z. Li, W. M. Wang, Y. Chen, and L. H. Zhu, 1999. Genetic analysis of rice grain quality. Theor. Appl. Genet. 98 : 502-508 https://doi.org/10.1007/s001220051098
  5. Hu, Z. L., P. Li, M. Q. Zhou, Z. H. Zhang, L. X. Wang, L. H. Zhu, and Y. G. Zhu, 2004. Mapping of quantitative trait loci (QTLs) for rice protein and fat content using doubled haploid lines. Euphytica. 135 : 47-54 https://doi.org/10.1023/B:EUPH.0000009539.38916.32
  6. Jin, F. X., K. H. Kang, S. J. Kwon, O. Y. Jeong, L. H. Le, H. P. Moon, and S. N. Ahn, 2004. Introgression of Oryza minuta into rice, O. sativa. Korean J. Crop Sci. 49(6) : 533-538
  7. Septiningsih, E. M., K. R. Trijatmiko, S. Moeljopawiro, and S. R. McCouch, 2003. Identification of quantitative trait loci for grain quality in an advanced backcross population derived from the Oryza sativa variety IR64 and the wild relative O. rufipogon. Theor. Appl. Genet. 107 : 1433-1441 https://doi.org/10.1007/s00122-003-1376-z
  8. Tan, Y. F., J. X. Li, S. B. Yu, Y. Z. Xing, C. G. Xu, and Q. Zhang, 1999. The three important traits for cooking and eating quality of rice grains are controlled by a single locus in an elite rice hybrid, Shanyou 63. Theor. Appl. Genet. 99 : 642-648 https://doi.org/10.1007/s001220051279
  9. Tan, Y. F., Y. Z. Xing, J. X. Li, S. B. Yu, C. G. Xu, and Q. Zhang, 2000. Genetic bases of appearance quality of rice grains in Shanyou 63, an elite rice hybrid. Theor. Appl. Genet. 101 : 823-829 https://doi.org/10.1007/s001220051549
  10. Tan, Y. F., M. Sun, Y. Z. Xing, J. P. Hua, X. L. Sun, Q. Zhang, and H. Croke, 2001. Mapping quantitative trait loci for milling quality, protein content and color characteristics of rice using a recombinant inbred line population derived from an elite rice hybrid. Theor. Appl. Genet. 103 : 1037-1045 https://doi.org/10.1007/s001220100665
  11. Xiao, J, J. Y. Li, and S. D. Tanksley, 1996. Identification of QTL affecting traits of agronomic importance in a recombinant inbred population derived from sub-specific rice cross. Theor. Appl. Genet. 92 : 230-244 https://doi.org/10.1007/BF00223380
  12. Xu, C. W., Mo, H. D., Zhang, A. H., Zhu, Q. S., 1995. Genetical control of quality traits of rice grains in indicajaponica hybrids. Acta Genet Sinica 22 : 192-198
  13. Yano, M. and T. Sasaki, 1997. Genetic and molecular dissection of quantitative traits in rice. Plant Molecular Biology. 35 : 145-153 https://doi.org/10.1023/A:1005764209331