• 임상간호연구
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• 제15권1호
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• pp.55-65
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• 2009

Purpose: The purpose of this study was to compare the 1% chlorhexidine gluconate/61% ethanol (CHG/ethanol), 45% ethanol/18% 1-propanol (ethanol/propanol) and 7.5% povidone-iodine (PVI) scrub with brush to evaluate their antimicrobial effect. Method: Utilizing repeated measures design, 9 nurses participated in the study. Glove juice sampling procedure was used to evaluate microbial hand counts before the surgical hand antisepsis, one minute after hand wash, and after the surgery. Results: Waterless rub using CHG and ethanol combination resulted in a 3.94 log reduction at 1 min and 2.78 log reduction at 3 hrs. Ethanol/propanol resulted in a 2.42 at 1 min and 2.22 at 3 hrs. The traditional scrub using PVI with brush resulted in a 0.94 at 1 min and 0.95 at 3 hrs (p=.003) and 3 hrs (p=.026) after the surgical hand antisepsis. Repeated measures ANOVA results showed that there was a statistically significant difference among group (p=.002). Duncan post hoc test result showed that the PVI was less effective (p<.05) in sterilizing microbials on hands than CHG/ethanol or ethanol/propanol. Conclusion: Both of the two alcohol-based antiseptic rubs are acceptable alternatives to the PVI with brush for surgical hand antisepsis.

• Korean Chemical Engineering Research
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• 제55권5호
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• pp.668-678
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• 2017

Intermolecular interactions were studied for binary mixtures of 2-propanol + cyclohexane, n-hexane, benzene, toluene, o-, m- and p-xylenes by measuring ultrasonic speeds (u) over the entire range of composition at 298.15 K and 308.15 K. From these results the deviation in ultrasonic speed was calculated. These results were fitted to the Redlich-Kister equation to derive the binary coefficients along with standard deviations between the experimental and calculated data. Acoustic parameters such as excess isentropic compressibility ($K_s^E$), intermolecular free length ($L_f$) and available volume ($V_a$) were also derived from ultrasonic speed data and Jacobson's free length theory. The ultrasonic speed data were correlated by Nomoto's relation, Van Dael's mixing relation, impedance dependence relation, and Schaaff's collision factor theory. Van Dael's relation gives the best prediction of u in the binary mixtures containing aliphatic hydrocarbons. The ultrasonic speed data and isentropic compressibility were further analyzed in terms of Jacobson's free length theory.

• Korean Chemical Engineering Research
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• 제43권1호
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• pp.181-185
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• 2005

인화점은 가연성 물질의 화재 및 폭발의 잠재위험성을 결정하는데 가장 중요한 연소 특성치 가운데 하나이다. 인화점의 정확한 지식은 산업 화재의 방호 평가 및 적절한 예방에 중요하다. 본 연구에서 n-butanol+n-propionic acid계와, n-propanol+n-propionic계의 개방계 인화점을 Tag식 개방계 장치(ASTM D 1310-86)를 이용하여 측정하였다. 실험값은 라울의 법칙과 van Laar식에 의해 계산된 값과 비교하였다. 그 결과, van Laar식에 의한 예측값이 라울의 법칙에 의한 예측값 보다 실험값에 더욱 근사함을 확인하였다.

• 한국식품위생안전성학회지
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• 제14권3호
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• pp.233-237
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• 1999

Lactobacillus sp. GM7311을 MRS broth에 배양하여 배양 상등액으로부터 n-propanol/acetone처리 , ion-exchange chromatography, gel filtration 및 SDS-PAGE를 이용해 박테이로신을 부분 정제하였다. 정제 과정 중 가장 큰 문제점은 n-propanol 과 acetone등의 유기용매 처리 단계에서의 급격한 활성 감소로서 회수율은 48.0%를 나타내었다. 거러나 이후 CM-cllulose를 통한 ion-exchange chromatography와 Sephacryl HR-100에 의한 gel filtration 과정을 거치면서 활성이 크게 증가하여, 비활성 뿐만 아니라 정제도에서도 493배의 증가를 얻을 수 있었으며 최종 회수율은 8.3%였다. SDS-PAGE로 정제도를 확인했을 때 분자량 약 8,200과 2,500의 2개의 band를 관찰할 수 있었으며, 2개의 band를 각각 나누어 활성을 측정한 결과 두 부분 모두 활성이 있는 것으로 나타나 Lactobacillus sp. GM7311이 2가지 성분의 박테리오신을 생산하는 것으로 추정할 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1465-1469
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• 2001

A sensitive high-performance liquid chromatographic method was developed to determine the content of cholesterol in milk and dairy products. To optimize separation of cholesterol, mobile phases including acetonitrile:2-propanol (8:1, v/v), acetonitrile:methanol (3:1, v/v), and acetonitrile:methanoI:2-propanol (7:3: I, v/v/v) were compared. Acetonitrile/methanol/2-propanol was superior to the other mobile phase systems for separating cholesterol. Liquid-liquid extraction (LLE) of cholesterol was simplified using a non-polar solvent, hexane, to remove interfering compounds, and had an excellent recovery $(100{\pm}1.0%)$ of cholesterol. A solid phase extraction (SPE) method using Sep-pak $C_{18}$ was developed and compared with LLE. The SPE method was rapid and highly reproducible. Both extraction methods were useful when used in combination with saponification of esterified cholesterol to facilitate total cholesterol determination. The detection limit of cholesterol was $0.01{\mu}g$. The newly developed HPLC method was rapid, simple, and accurate, and has advantages over the many methods commonly used.

• 한국미생물·생명공학회지
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• 제27권2호
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• pp.124-128
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• 1999

TK6 was able to produce NAD+ dependent cyclohexanol dehydrogenase(CDH). The production of CDH was increased rapidly at the logarithmic phase and maintained constantly after that. In order to investigate the inductive production of CDH by various substrates, the bacteria were grown in the media containing alicyclic hydrocarbons and various alcohols as a sole crabon souce. CDH was induced most actively by cyclohexanol. Cyclohexanone and cyclohexane-1,2-diol also induced remarkable amount of CDH but it was induced weakly by 1-propanol, 1-butanol, 1-pentanol, 1-hexanol, 2-propanol, and 2-methyl-1-propanol. The dehydrogenase of the bacteria grown in the media containing cyclohexanol were weakly active for various alcohols, but the dehydrogenase activity for cyclohexane-1,2-diol was twice as much as that for cyclohexanol. Activity staining on PAGE of the cell free extract of Rhodococcus sp. TK6 grown in the media containing cyclohexanol reveals at least sever isozyme bands of CDH and we nominated the four major activity bands as CDH I, II, III, and IV. CDH I was strongly induced by cyclohexanol, cyclohexane-1,2-diok, but its activity was specific to cyclohexane-1,2-diol and 1-pentanol. CDH IV was strongly induced by cyclohexanol and cyclohexane-1,2-diol, and its activity was very specific to cyclohexane-1,2-diol.

• Korea-Australia Rheology Journal
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• 제20권1호
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• pp.1-6
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• 2008

Absorption rate of carbon dioxide was measured in the aqueous xanthan gum (XG) solution in the range of 0-0.15 wt% containing 2-amino-2-methyl-1-propanol (AMP) of $0-2\;kmol/m^3$ in a flat-stirred vessel with an impeller of 0.05m and agitation speed of 50rpm at $25^{\circ}C$ and 0.101 MPa. The volumetric liquid-side mass transfer coefficient ($k_La$) of $CO_2$, which was correlated with the viscosity and the elastic behavior of XG solution containing Deborah number as an empirical formula, was used to estimate the chemical absorption rate of $CO_2\;(R_A)$. $R_A$, which was estimated by mass transfer mechanism based on the film theory using the physicochemical properties and the kinetics of reaction between $CO_2$ and AMP, was compared with the measured rate. The aqueous XG solution with elastic property of non-Newtonian liquid made $R_A$ increased compared with Newtonian liquid based on the same viscosity of the solution.

• 대한화학회지
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• 제37권8호
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• pp.753-756
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• 1993

디메틸술록시드와 물 및 몇가지 알칸올간의 분자 상호작용에 관한 연구를 증기압 삼투법으로 40$^{\circ}C$에서 수행하였다. 디메틸술폭시드는 물, 메탄올, 에탄올, 1-프로판올, 2-프로판올과 2-메틸-1-프로판올에서는 라울의 법칙에서 부의 벗어남을 나타내었으며, 1-부탄올과 1-펜탄올에서는 라울의 법칙에서 정의 벗어남을 나타내었다. 이 결과를 이종분자간의 상호작용과 DMSO 분자의 자체회합으로 각각 해석하였다. 측정한 물과 알칸올과의 히드록실 양성자의 화학적 이동도 이 결과를 뒷받침하였다.

• Bulletin of the Korean Chemical Society
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• 제26권10호
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• pp.1549-1554
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• 2005

Chiral separation of arylalcohols such as 1-phenyl-1-propanol, 1-phenyl-2-propanol, and 2-phenyl-1-propanol by capillary electrophoresis was studied using sulfonated $\beta$-cyclodextrin (CD) as a chiral selector and Ag colloids as an additive. The optimum separation condition of arylalcohols was found to be the chiral selector concentration of 6.5 mM, applied voltage of 15 kV, and pH of 7.0. In order to improve chiral separation, an Ag colloid was mixed with a running buffer. The resolution in the Ag colloid-mixed running buffer was considerably superior to that obtained with the sulfonated $\beta$-CD alone. The molar ratio of sulfonated $\beta$-CD to Ag colloid, which is one of critical parameters affecting resolution, was found to be optimum at 65 : 1. In order to elucidate the resolution mechanism, an inclusion-complex of the arylalcohols with sulfonated $\beta$-CD was prepared by mixing and shaking in solution, and then characterized by cyclic voltammetry (CV). The inclusion mechanism was also discussed using experimental results.

• 한국가스학회지
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• 제18권5호
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• pp.60-65
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• 2014

가연성 액체 혼합물의 화재 위험성을 특징짓는 주된 물리적 성질은 인화점이다. 본 연구는 이성분계 가연성 혼합물의 인화점을 측정하고 예측하고자 한다. Seta flash 밀폐식 장치를 이용하여 n-propanol+propionic acid 계의 인화점을 측정하였다. van Laar 식과 NRTL 식을 이용한 최적화 기법을 통해 인화점을 예측하였다. 이 계산치와 라울의 법칙에 의한 계산치를 비교하였다. 그 결과, 최적화 기법에 의한 계산치가 라울의 법칙에 의한 계산치 보다 측정값에 보다 근사하였다.