• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제30권3호
• /
• pp.186-192
• /
• 2004

Purpose: Human tooth proteins are highly heterogeneous, comprising diverse proteins derived from a number of genes. The attempts to identify protein for activity of tooth matrix proteins have been defied by several factors. First, the amount of proteins within teeth is very small relative to many extracellular matrix proteins of other tissues. Second, the bioassay system is tedious and needed for long time. Therefore we tried to find easy techniques, which increase the product rate, and an assay of small proteins, with which amino acid sequence is possible without additional procedures. Materials and Methods: Total protein were extracted from 300 g enamel removed teeth and 600 g teeth with 4 mol/L guanidine HCl and purified by gel chromatography. Aliquot of proteins was implanted into muscle pouches in Sprague-Dawley rats for bioassay. By SDS-PAGE and membrane blotting, molecular weight of each protein was estimated and a partial amino acid sequence was obtained. Each fraction blotted on the membrane was cut out and inserted in rat ectopic model. Results: In dissociative method, total tooth proteins were obtained 1mg/ml from enamel removed teeth and 3.5 mg/ml from teeth. In SDS-PAGE, four clear bands at the sites corresponding to 66, 40, 20 and 18 kD. Especially The 66 kD band was clearly exhibited. Amino acid sequencing from tooth could be possible using PVDF membrane blotting technique. In amino acid sequencing, 66 kD protein was identified as albumin. Conclusion: Compared with conventional method for extraction of teeth protein and bioassay of proteins, the methods in this study were easy, time-saving and more productive technique. The matured tooth proteins omitting additional procedure of mechanical removal of enamel were simply analyzed using blotted PVDF membrane. This method seems to make a contribution as a technique for bioassay and amino acid sequencing of protein.

• Mycobiology
• /
• 제46권3호
• /
• pp.269-277
• /
• 2018

The production of water-soluble pigments by fungal strains indigenous to South Korea was investigated to find those that are highly productive in submerged culture. Among 113 candidates, 34 strains that colored the inoculated potato dextrose agar medium were selected. They were cultured in potato dextrose broth and extracted with ethanol. The productivity, functionality (radical-scavenging activities), and color information (CIELAB values) of the pigment extracts were measured. Five species produced intense yellowish pigments, and two produced intense reddish pigments that ranked the highest in terms of absorbance units produced per day. The pigment extracts of Penicillium miczynskii, Sanghuangporus baumii, Trichoderma sp. 1, and Trichoderma afroharzianum exhibited high radical-scavenging activity. However, the S. baumii extract showed moderate toxicity in the acute toxicity test, which limits the industrial application of this pigment. In conclusion, P. miczynskii KUC1721, Trichoderma sp. 1 KUC1716, and T. afroharzianum KUC21213 were the best fungal candidates to be industrial producers of safe, functional water-soluble pigments.

• 디지털융복합연구
• /
• 제11권11호
• /
• pp.499-508
• /
• 2013

본 연구는 빠른 속도로 증가하고 있는 농촌 노인의 성공적 노화를 위한 중재 프로그램 개발의 기초자료를 얻고자 농촌 노인들의 성공적 노화의 영향요인을 조사한 서술적 조사연구이다. 자료 수집은 2012년 9월부터 12월까지 W군에 거주하는 65세 이상 노인 208명을 편의추출하여 설문지를 통해 자료를 수집하였다. 자료분석은 SPSS 21.0 program을 사용하였으며, independent t-test, one-way ANOVA, Pearson's correlation coefficient, Multiple regression으로 분석하였다. 연구결과 농촌 노인의 성공적 노화에 영향을 주는 설명요인으로 사회적 지지, 우울, 인지기능, 생산적 활동, 일상생활활동 순서로 나타났으며, 설명력은 42.1%였다. 농촌 노인의 성공적 노화를 위해서는 지역사회와의 연계를 통한 보건의료서비스제공 및 사회적 활동 등 사회적 지지체계 구축이 가장 필요하며, 이에 따른 맞춤형 간호 중재 프로그램 개발의 필요성을 제언한다.

• Molecules and Cells
• /
• 제40권1호
• /
• pp.24-36
• /
• 2017

The stability of peptide-MHC complex (pMHC) is an important factor to shape the fate of peptide-specific T cell immune response, but how it influences on T cell activation process is poorly understood. To better understand that, we investigated various T cell activation events driven by $L^d$ MHCI loaded with graded concentrations of P2Ca and QL9 peptides, respectively, with 2C TCR Tg T cells; the binding strength of P2Ca for $L^d$ is measurably weaker than that of QL9, but either peptides in the context of $L^d$ interact with 2C TCR with a similar strength. When their concentrations required for early T cell activation events, which occur within several minutes to an hour, were concerned, $EC_{50}s$ of QL9 were about 100 folds lower than those of P2Ca, which was expected from their association constants for $L^d$. When $EC_{50}s$ for late activation events, which takes over several hours to occur, were concerned, the differences grew even larger (> 300 folds), suggesting that, due to weak binding, $L^d/P2Ca$ dissociate from each other more easily to lose its antigenicity in a short time. Accordingly, fixation of $L^d/P2Ca$ with paraformaldehyde resulted in a significant improvement in its immunogenicity. These results imply that binding strength of a peptide for a MHC is a critical factor to determine the duration of pMHC-mediated T cell activation and thus the attainment of productive T cell activation. It is also suggested that paraformaldehyde fixation should be an effective tool to ameliorate the immunogenicity of pMHC with a poor stability.

• 한국산학기술학회논문지
• /
• 제12권4호
• /
• pp.1833-1843
• /
• 2011

최근 우리사회는 사회경제적으로 급격한 변화를 맞이하고 있다. 세계에서 가장 빠른 속도로 고령화가 진행되는 가운데 베이비붐 세대의 퇴직이 본격화되고 있다. 이러한 베이비붐 세대는 지난 70~80년대 우리경제발전의 주역으로 퇴직 후 재취업의 현실적 어려움과 미흡한 노후준비 및 이들의 복지와 의료문제 등이 국가적 현안으로 대두되고 있다. 이에 정부는 금년부터 시니어의 강점을 적극 활용하여 생산적 경제활동 참여를 위해 시니어창업육성정책을 수립하고 있다. 그러나 이러한 시니어창업의 중요성에도 불구하고 시니어의 욕구와 시니어창업의 구체적인 특성을 규명하는 연구는 전무한 실정이다. 이에 본 연구에서는 시니어의 구체적인 특성과 창업의도 및 성공창업의 핵심역량인 기업가정신에 미치는 영향을 실증적으로 분석하고 시니어창업의 활성화 방안을 제시하였다.

• Animal Bioscience
• /
• 제34권3_spc호
• /
• pp.443-448
• /
• 2021

Objective: Astaxanthin is a natural super antioxidant. The present study was carried out to investigate the effect of astaxanthin rich Phaffia rhodozyma (PR) supplementation in diets on laying production performance, egg quality, antioxidant defenses and immune defenses in laying hens. Methods: A total of five hundred and twelve 60-week-old Lohmann Brown laying hens (2,243±12 g) were randomly assigned to four groups, each including 4 replicates with 32 birds per replicate. Astaxanthin rich PR was added to corn-soybean meal diets to produce experimental diets containing 0 (Control), 800 mg/kg, 1,200 mg/kg, and 1,600 mg/kg PR, respectively. The astaxanthin content in the diet was 0.96 mg/kg, 1.44 mg/kg and 1.92 mg/kg respectively. Results: Results showed that dietary PR supplementation tended to increase daily feed intake (p = 0.0512). There was no effect of astaxanthin rich PR on Haugh units, albumen height, egg shape index, eggshell strength, and eggshell thickness at weeks 6 (p>0.05). However, egg yolk color was significantly improved (p<0.05). In addition, astaxanthin rich PR supplementation significantly increased serum glutathione peroxidase and superoxide dismutase activity (p<0.05), increased serum immunoglobulin G content (p<0.05), and reduced malondialdehyde content (p<0.05) in laying hens. Conclusion: In conclusion, astaxanthin rich PR can improve the color of egg yolk, enhance the antioxidant defenses, and regulate the immune function.

• 한국환경과학회지
• /
• 제32권12호
• /
• pp.955-964
• /
• 2023

This study was conducted to obtain basic data on efficient and eco-friendly crop cultivation for urban residents who enjoy urban agriculture as a hobby or leisure activity. We planted strawberry(Fragaria x ananassa)(S) and kidney bean (Phaseolus vulgaris var. humilis)(K) in different ratios to analyze the differences in soil environment, growth, physiology, and productivity, and to investigate the effects. Strawberry growth was optimal with S1K2 ratio, whereas the S1K1 ratio treatment showed the highest levels of physiology and productivity. In terms of growth and physiology, kidney beans tended to perform best in S1K2 treatment. The average number of productive pods was two, with a highest average value of 2.3 being recorded in S2K1 treatment planted with a high percentage of strawberries. In terms of growth, physiology, and prodctivity our findings indicate that it would be desirable to plant starwberries and kidney bean in a 1:1 ratio. However, considering the environmental characteristics of walls and rooftops, it is necessary to effectively manage crops suitable for these conditions. Furthermore, additional studies should be conducted to analyze the quality of fruits and seeds producted, both qualitatively and quantitatively in the future.

• Journal of Veterinary Science
• /
• 제25권4호
• /
• pp.42.1-42.12
• /
• 2024

Importance: Bovine mastitis, predominantly associated with gram-positive Staphylococcus aureus, poses a significant threat to dairy cows, leading to a decline in milk quality and volume with substantial economic implications. Objective: This study investigated the incidence, virulence, and antibiotic resistance of S. aureus associated with mastitis in dairy cows. Methods: Fifty milk-productive cows underwent a subclinical mastitis diagnosis, and the S. aureus strains were isolated. Genomic DNA extraction, sequencing, and bioinformatic analysis were performed, supplemented by including 124 S. aureus genomes from cows with subclinical mastitis to enhance the overall analysis. Results: The results revealed a 42% prevalence of subclinical mastitis among the cows tested. Genomic analysis identified 26 sequence types (STs) for all isolates, with Mexican STs belonging primarily to CC1 and CC97. The analyzed genomes exhibited multidrug resistance to phenicol, fluoroquinolone, tetracycline, and cephalosporine, which are commonly used as the first line of treatment. Furthermore, a similar genomic virulence repertoire was observed across the genomes, encompassing the genes related to invasion, survival, pathogenesis, and iron uptake. In particular, the toxic shock syndrome toxin (tss-1) was found predominantly in the genomes isolated in this study, posing potential health risks, particularly in children. Conclusion and Relevance: These findings underscore the broad capacity for antibiotic resistance and pathogenicity by S. aureus, compromising the integrity of milk and dairy products. The study emphasizes the need to evaluate the effectiveness of antibiotics in combating S. aureus infections.

• 한국전자통신학회논문지
• /
• 제8권6호
• /
• pp.933-940
• /
• 2013

우리나라는 지금 고령사회의 도전에 직면해 있다. 젊은 경제활동인구가 감소하고 노인인구가 크게 증가하는 고령사회에서는 전대미문의 노인문제가 제기된다. 노인 인구가 급작스럽고도 빨리 증가하는 사회는 고령화의 쇼크, 고령화 사회의 지진 등으로 불리는 미래사회의 위기를 경고하고 있다. 한국의 노인들은 여러 사회적인 요인으로 인해 제대로 교육을 받지 못한 집단으로 어느 구성원 보다 평생교육이 필요한 집단이며 노인들은 평생교육의 주요 대상자로 부각되고 있다. 현대사회는 문화수준의 향상과 함께 삶이 윤택해지고 삶에 대한 개인적인 욕구가 높아지면서 여가생활의 필연성이 강조되고 있고, 여가스포츠를 통해 단순히 스트레스만을 해소하기 보다는 그것을 통해 교육의 기회를 제공받고자 한다. 또한 노인 여가스포츠 활동은 21세기에 요구되는 노인상, 즉 주체적인 노인, 생산적인 노인, 봉사하는 노인, 정보화된 노인, 통합적인 노인으로 노인의 정체성을 확립하기 위한 중요한 수단이 되어야 하고 여가스포츠를 체계적으로 제공하는 전문 여가스포츠 프로그램이 평생 교육적 차원에서 이루어져야 할 것이다. 그러므로 노인의 평생 교육적 차원으로써 여가스포츠는 건강증진과 사회성 함양, 그리고 자아실현과 함께 노인의 삶의 질을 향상시키는데 큰 의미가 있다고 할 수 있으며, 21세기가 요구하는 자립적인 생활을 영위하며 남에게 의존하지 않고 당당하게 독립적으로 살아가는 디지털 시대의 성공적인 신(新)노인상을 정립 하는데 큰 의미가 있을 것이다.

• 서비스연구
• /
• 제7권2호
• /
• pp.1-16
• /
• 2017

비생산적 활동이며 제조의 보조 활동으로 인식되었던 서비스가 '서비스' 자체로 고객의 수요를 이끌어내는 동력이 되면서 서비스 활동의 저변이 확대되고 서비스가 빠르게 진화하고 있다. 서비스시스템을 이해하기 위해 서비스 개념의 변화를 살펴보는 것은 중요하다. 서비스 시스템 자체가 서비스 개념을 기반으로 한 순환적 성격을 띠고 있으므로, 서비스 개념의 진화에 따라 서비스 시스템이 어떻게 변화하고 있는지를 살펴봄으로써 서비스를 '어떻게' 가치 있게 활용할 수 있는지 연구할 수 있기 때문이다. 관계를 구성하고 활용하는 능력은 서비스경제시대에 경영자에게 더욱 중요한 핵심 요인으로 꼽히고 있으나, 여전히 기업 경영자들의 관심은 내부적 역량에 초점이 맞추어져 있고, 외부자원(고객의 지식과 역량 등) 활용에 익숙하지 않은 실정이다. 이에 본 연구에서는 서비스 유형별 사례연구를 통해 서비스 관계 속에서 상호 작용하는 서비스 공급자-수요자들의 활동을 분석하고, 무형적 가치와 장기적 효과(Outcome)를 강조하는 신 서비스시스템모델을 구축하였다. 본 연구는 오늘날의 서비스경제시대에 맞는 고객의 역할을 재조명하고, 기업의 경영 성과를 위해 신 서비스 모델이 적극 활용될 수 있는 가치가 있다.