• Journal of Science Education
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• v.45 no.3
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• pp.292-303
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• 2021

This study identifies the terms frequently used together with energy in online science news articles and topics of the news reports to find out how the term energy is used in everyday life and to draw implications for science curriculum and instruction about energy. A total of 2,171 online news articles in science category published by 11 major newspaper companies in Korea for one year from March 1, 2018 were selected by using energy as a search term. As a result of natural language processing, a total of 51,224 sentences consisting of 507,901 words were compiled for analysis. Using the R program, term frequency analysis, semantic network analysis, and structural topic modeling were performed. The results show that the terms with exceptionally high frequencies were technology, research, and development, which reflected the characteristics of news articles that report new findings. On the other hand, terms used more than once per two articles were industry-related terms (industry, product, system, production, market) and terms that were sufficiently expected as energy-related terms such as 'electricity' and 'environment.' Meanwhile, 'sun', 'heat', 'temperature', and 'power generation', which are frequently used in energy-related science classes, also appeared as terms belonging to the highest frequency. From a network analysis, two clusters were found including terms related to industry and technology and terms related to basic science and research. From the analysis of terms paired with energy, it was also found that terms related to the use of energy such as 'energy efficiency,' 'energy saving,' and 'energy consumption' were the most frequently used. Out of 16 topics found, four contexts of energy were drawn including 'high-tech industry,' 'industry,' 'basic science,' and 'environment and health.' The results suggest that the introduction of the concept of energy degradation as a starting point for energy classes can be effective. It also shows the need to introduce high-tech industries or the context of environment and health into energy learning.

• Journal of Bio-Environment Control
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• v.31 no.1
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• pp.14-21
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• 2022

Uniform scions and rootstocks should be produced to ensure grafting success. Light quality is an important environmental factor that regulates seedling growth. The effects of warm- and cool-white light emitting diode (LED) ratios on seedling growth were investigated. Scions and rootstocks of cucumber, tomato, and watermelon were grown in a closed transplant production system using LED as the sole lighting source. The LED treatments were W1C0 (only warm-white), W1C1 (warm-white: cool-white = 1:1), W3C1 (warm-white: cool-white = 3:1), and W5C2 (warm-white: cool-white = 5:2). The seedlings grown in W1C1 had the shortest hypocotyls, and the seedlings grown in W1C0 had the longest hypocotyls among the three tested vegetables. The hypocotyls of watermelon scions, watermelon rootstocks, and tomato rootstocks were shortest in W1C1, followed by those in W3C1, W5C2, and W1C0, but there was no significant difference between W3C1 and W5C2, which remained the same as the ratio of cool-white LEDs increased. In addition, tomato scions had the first and second longest hypocotyls in W1C0 and W3C1, respectively, and the shortest hypocotyls in W5C2 and W1C1, along with W5C2 and W1C1, although the difference was not significant. The stem diameter was highest in W1C0 except for tomato seedlings and rootstocks of watermelon. The shoot fresh weight of scions and rootstocks of cucumber and watermelon and the root fresh weight of cucumber scions were lowest in W1C1. These results indicated that different ratios of LED lighting sources had a strong effect on the hypocotyl elongation of seedlings.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.40 no.3
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• pp.1-9
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• 2022

This study compared and reviewed the recognition determinants by applying the OECMs criteria, focusing on old big trees, plant of natural monument that are natural heritage under the national heritage system of the Cultural Heritage Administration, and the results are as follows. First, among the protected areas designated and managed by government agencies according to each protection purpose, it is necessary to actively introduce new conservation measures, OECMs, to fulfill the Biodiversity strategy for 2030 while the land area is already saturated. Second, the OECMs are geographically defined areas(CBD, 2018), not currently recognized as a protected areas, governed and managed in a way that achieves positived sustained and effective contribution to in situ conservation of biodiversity. Since the selection of term, the scope of application criteria, and the context of interpretation are inevitably different, it is necessary to separately legislate and establish related laws of the OECMs suitable for each country's situation. Third, as a result of reviewing the OECMs criteria for plant of natural monument, the final 58 potential resources were recognized. Important elements among the OECMs criteria are that buffer zones should be spaced apart from designated zones to secure a certain area, and that economic activities through commercial production should not occur and meet biodiversity standards. Among the potential candidates, 23 areas were analyzed to be geographically isolated and independent, such as Forest of Oriental Arborvitae in Do-dong, Daegu, and forest types such as Carstor Aralia of Gungchon-ri, Samcheok and Forest of Common Camellias in Maryang-ri, Seocheon. As a result of reviewing the application of OECMs criteria for plant of natural monument, it was confirmed that the functions as a traditional uses were specialized among the values of biodiversity, and ecosystem services and cultural and spiritual values were inherited through Korea's unique culture of old big trees and Dangsan ritual. In terms of biodiversity criteria, it can be used as an important factor in connecting human and natural ecosystem networks without the discovery of new species.

• Journal of fish pathology
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• v.35 no.1
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• pp.1-25
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• 2022

The aquaculture industry has developed rapidly over the last three decades and is an important industry that supplies over 15% of humans' animal protein intake; therefore, there is a need to increase production to meet the continuous demand. The fish cage farms on the southern coast (Kyengsangnam-do and Jeollanam-do) of Korea are critical resources in aquaculture because they account for approximately 90% of the national total fish cage farms by water area ratio. However, the current aquaculture environment is being gradually affected by climate change, which is a global issue, and its effects are expected to intensify in the future. Therefore, it is urgently imperative to accurately evaluate the effects of climate change on South Korean aquaculture industries and to develop social and national strategies to minimize damage to the fishing industry. The damage to fish farmed in cage farms on the southern coast is increasing annually and the leading causes are high and low water temperature and red tides, which are directly or indirectly related to climate change. At present, global warming can provide opportunities for aquaculture industrialization of fish or other novel species, with economic implications. However, despite such opportunities, the influx of new species can also cause problems such as ecological disturbances, increase in the reproduction frequency of microalgae such as red tide, increase in disease incidence, and occurrence and periods of high water temperatures in summer. The scale of farmed fish mortality is increasing due to the complex effects of these factors. Increased damages due to fish mortality not only have severe economic impacts on the aquaculture industry, but the social costs of responding to the damage and follow-up measures also increase. various active responses can reduce the mortality damage in fish farms such as improving the management skills in aquaculture, improved species breeding, efficient food management, disease prevention, proactive responses, and system-wide improvements. This review article analyzes the large-scale mortality cases occurring in fish cage farms on the southern coast of Korea and proposes measures to mitigate mortality and enhance responses to such scenarios.

• The Korean Journal of Archival Studies
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• no.73
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• pp.161-188
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• 2022

This article explores the current status of arrangement of the archives relating to the Korean Provisional Assembly held by the National Assembly Library and suggests the direction of rearrangement focusing on the principles of arrangement. The Korean Provisional Assembly had records management regulations, and records were produced and stored according to them. However, the archives lost their original order at some point. The National Assembly Library collected and managed them in the 1960s. The National Assembly Library did not fully consider the records management system at the time of record production and various situations that may occur during the storage process while organizing the collected archives. At that time, the National Assembly Library did not follow the records management regulations of the Korean Provisional Assembly. In addition, the hierarchical structure of archives was not applied during the arrangement, and the National Assembly Library arranged without considering the Principal of Provenance and the Principle of original order. As a result, it became difficult to understand the structure and context of the archives. In order to solve these problems and come up with a plan for rearranging the archives, first of all, it is necessary to examine the characteristics of the records related to the Korean Provisional Assembly in accordance with the principles of record arrangement. First, according to the Principal of Provenance, it is necessary to identify the organization, function, and records and classify the records item, records file, creators, dates of creation, types of records etc. Second, by applying the Principle of original order, it is necessary to understand what the order of records was at the time when records were created and preserved. Third, it is necessary to examine whether the records are completely created and valid. It is impossible to completely arrange the archives related to the Korean Provisional Assembly as it was in the past. However, by examining the current state of arrangement and the direction of rearrangement, it will be possible to newly understand the contents, structure, and context of the archives and create a basis for effective reference service.

• Journal of the Korean Institute of Landscape Architecture
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• v.50 no.4
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• pp.20-36
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• 2022

This study aims to provide basic data for high-quality street tree management by setting reasonable management items and appropriate unit prices by reviewing the adequacy of current street tree management. Currently, street tree management items, except for street tree pruning, use general landscape tree quantity per unit for the street tree management quantity per unit. KEPCO (Korea Electric Power Corporation) applied pruning items from standard electric production infrastructure and carried out the activities at an average unit price of 51% lower for heavy pruning and 39% lower for light pruning than the standard estimate. This was judged to be a level that could not maintain or increase the quality of street tree management. It was determined that an appropriate standard unit price for street tree management was necessary. To improve the quantity per unit for the proper management of street trees, it was necessary to review costs in the field. However, due to the absence of data on actual construction costs in the domestic landscape field, detailed items of the US RSMeans Building Construction Cost Data (RSMeans) were reviewed, and the actual construction costs were calculated by applying personal domestic expenses. As a result, the standard of the estimated unit showed a good ratio of 107% for heavy pruning of street tree pruning compared to the actual construction cost, but light pruning was underestimated with a 59% ratio. Shrub pruning was 82%, weeding was 92%, tree fertilization was 87%, and windbreak wall installation was 91% under-engineered. In addition, it was also confirmed that the watering by sprinkler trucks and chemical spraying were over-designed compared to the actual construction cost at the rates of 118% and 124%, respectively. Due to the specificity of the street trees, the increase in personal expenses and the input cost of equipment, such as road safety controls, were judged to be the main cause of the underestimation of items. Therefore, it is necessary to add items related to street trees and general landscape trees to the landscape maintenance items of the standard of the estimated unit.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.4
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• pp.253-264
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• 2022

In this study, we investigated the occurrence of viral diseases in the early growth stage of soybean to establish management practices. We collected 83 soybean samples showing abnormal symptoms, approximately 3-4 weeks after seeding in the breeding field of the National Institute of Crop Science. Viruses were detected in the collected samples using reverse transcription polymerase chain reaction (RT-PCR) and metatranscriptome analysis of all those samples. The incidence of viral diseases in the field was less than 1% overall and up to 50% in certain cultivars and lines. RT-PCR and metatranscriptome analysis detected Soybean yellow mottle mosaic virus (SYMMV), Soybean mosaic virus (SMV), Soybean yellow common mosaic virus, Peanut stunt virus, and soybean geminivirus A (SGVA). Among these detected viruses, SYMMV and SMV were identified as major viruses causing infection in the early growth stage of soybean, with detection rates of 53.7% and 42.6%, respectively. Soybeans infected with SYMMV showed typical mosaic symptoms, whereas those infected with SMV showed a variety of symptoms such as mosaic, mottle, stunt, and chlorotic spots. Transmission characteristics of these viruses are variable, such that SMV is primarily transmitted by seeds, whereas SYMMV could be transmitted by insects, soil, and seeds. In this study, SGVA was detected in the early growth stage of soybean, and research on the current status and its effects on soybean after the early growth stage should be conducted.

• Journal of the Korea Organic Resources Recycling Association
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• v.31 no.3
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• pp.73-82
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• 2023

This study was conducted to investigate the effect of soil physico-chemical properties and crop growth responses for application of biochar derived from substrate with post harvest of oyster mushroom. The biochar was produced at 450~600℃ using a top-light up draft gasifier (TLUD) production system. As a result of elemental analysis, the biochar used was C 76.2%, H 2.5%, N 3.2%, and H/C was 0.39, which met the international certification standards for biocarbons (IBI) below 0.7. The chemical properties were 10.1 for pH, 1.0% for P₂O₅, 1.8% for K₂O, and 2.5% for CaO. The application rates of biochar were 0, 100, 200, 300, and 500 kg/10a. For cultivation of chinese cabbage and welsh onion, soil organic matter (OM), total nitrogen (T-N), total carbon (T-C), Ex.cation K contents and cation exchange capacity (CEC) in the treatments were increased compared to the no treatment. In addition, the bulk density was lowered and the porosity was increased, improving the soil physical properties in the treated soil. The growth of chinese cabbage and green onion increased with the application of biochar, but the yields of chinese cabbage and green onion did not significantly different among the treatments. Soil carbon sequestration in the treatments enhanced with increasing the amount of biochar application. It is expected to apply the biochar derived from spent oyster mushroom substrate in the eco-friendly farm soil management, improving soil physico-chemical properties.

• Biomolecules & Therapeutics
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• v.30 no.2
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• pp.203-211
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• 2022

Melanogenesis is the production of melanin from tyrosine by a series of enzyme-catalyzed reactions, in which tyrosinase and DOPA oxidase play key roles. The melanin content in the skin determines skin pigmentation. Abnormalities in skin pigmentation lead to various skin pigmentation disorders. Recent research has shown that the expression of EMP2 is much lower in melanoma than in normal melanocytes, but its role in melanogenesis has not yet been elucidated. Therefore, we investigated the role of EMP2 in the melanogenesis of MNT1 human melanoma cells. We examined TRP-1, TRP-2, and TYR expression levels during melanogenesis in MNT1 melanoma cells by gene silencing of EMP2. Western blot and RT-PCR results confirmed that the expression levels of TYR and TRP-2 were decreased when EMP2 expression was knocked down by EMP2 siRNA in MNT1 cells, and these changes were reversed when EMP2 was overexpressed. We verified the EMP2 gene was knocked out of the cell line (EMP2 CRISPR/Cas9) by using a CRISPR/Cas9 system and found that the expression levels of TRP-2 and TYR were significantly lower in the EMP2 CRISPR/Cas9 cell lines. Loss of EMP2 also reduced migration and invasion of MNT1 melanoma cells. In addition, the melanosome transfer from the melanocytes to keratinocytes in the EMP2 KO cells cocultured with keratinocytes was reduced compared to the cells in the control coculture group. In conclusion, these results suggest that EMP2 is involved in melanogenesis via the regulation of TRP-2 expression.

• Journal of Ginseng Research
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• v.47 no.3
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• pp.408-419
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• 2023

Background: Ginsenoside compound K (CK), the main active metabolite in Panax ginseng, has shown good safety and bioavailability in clinical trials and exerts neuroprotective effects in cerebral ischemic stroke. However, its potential role in the prevention of cerebral ischemia/reperfusion (I/R) injury remains unclear. Our study aimed to investigate the molecular mechanism of ginsenoside CK against cerebral I/R injury. Methods: We used a combination of in vitro and in vivo models, including oxygen and glucose deprivation/reperfusion induced PC12 cell model and middle cerebral artery occlusion/reperfusion induced rat model, to mimic I/R injury. Intracellular oxygen consumption and extracellular acidification rate were analyzed by Seahorse multifunctional energy metabolism system; ATP production was detected by luciferase method. The number and size of mitochondria were analyzed by transmission electron microscopy and MitoTracker probe combined with confocal laser microscopy. The potential mechanisms of ginsenoside CK on mitochondrial dynamics and bioenergy were evaluated by RNA interference, pharmacological antagonism combined with co-immunoprecipitation analysis and phenotypic analysis. Results: Ginsenoside CK pretreatment could attenuate mitochondrial translocation of DRP1, mitophagy, mitochondrial apoptosis, and neuronal bioenergy imbalance against cerebral I/R injury in both in vitro and in vivo models. Our data also confirmed that ginsenoside CK administration could reduce the binding affinity of Mul1 and Mfn2 to inhibit the ubiquitination and degradation of Mfn2, thereby elevating the protein level of Mfn2 in cerebral I/R injury. Conclusion: These data provide evidence that ginsenoside CK may be a promising therapeutic agent against cerebral I/R injury via Mul1/Mfn2 mediated mitochondrial dynamics and bioenergy.