• Mycobiology
• /
• 제38권3호
• /
• pp.180-183
• /
• 2010

This study was conducted out to select a silkworm variety suitable for synnemata production of Isaria tenuipes. Four kinds of the mulberry silkworm varieties, Bombyx mori, were hybridized using a Japanese parental line and a Chinese parental line, and used to test for synemata formation in I. tenuipes. The larval period of normal silkworms was 22 hr longer than the silkworms inoculated with this fungus. Among the silkworm varieties tested, Hachojam had the shortest larval period with 23.02 days. The non-cocooning silkworm had a shorter larval period than the cocoon producing silkworms. The pupation rate of normal silkworms was about 9% higher than that of silkworms sprayed with I. tenuipes. Hachojam had the highest infection rate at 99.8%, but no significant difference was observed for the infection rate by silkworm variety. The production of synnemata was the best in JS171 $\times$ CS188 with an incidence rate of 99.3%, followed by Hachojam, and Chugangjam. The synnemata produced from Hachojam were the heaviest and showed white or milky-white in color.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권2호
• /
• pp.287-292
• /
• 2018

Objective: The aim of present study was to assess the anti-oxidant potential of water-soluble peptides (WSPs) extract derived from buffalo and cow milk Cheddar cheeses at different stages of ripening. Methods: The antioxidant potential of WSPs extract was assessed through 2,2'-azinobis-3-ethylbenzothiazoline-6sulfonic acid (ABTS)-radical scavenging activity. In addition, impact of WSPs extract on cell viability and production of reactive oxygen species (ROS) in human colon adenocarcinoma Caco-2 (tert-butylhydroperoxide-induced) cell lines was also evaluated. Results: The ABTS-radical scavenging activity increased progressively with ripening period and dose-dependently in both cheeses. However, peptide extract from buffalo milk Cheddar cheese demonstrated relatively higher activity due to higher contents of water-soluble nitrogen. Intracellular ROS production in Caco-2 cells decreased significantly (p<0.05) till 150th day of cheese ripening and remained constant thereafter. Additionally, dose-dependent response of WSPs extract on antioxidant activity was noticed in the Caco-2 cell line. Conclusion: On the basis of current in vitro study, the Cheddar cheese WSPs extract can protect intestinal epithelium against oxidative stress due to their antioxidant activity.

• 대한용접접합학회:학술대회논문집
• /
• 대한용접접합학회 2002년도 Proceedings of the International Welding/Joining Conference-Korea
• /
• pp.181-186
• /
• 2002

Laser welding application for car body manufacturing has many advantages in the stiffness and the lightness of vehicle, the productivity of assembly line, and the degree of freedom in design. This presentation will express the innovation of car body manufacturing including parameter optimization, process modeling, and system integration. In this application the investment for systems was cut down dramatically by real time switching over the laser path between two welding stations. Points of technical discussion are as follows: optimization of parameters such as laser power, robot speed and trajectory, compact and useful design of jig & fixture to assure welding quality for 3 sheet-layer zinc-coated steel, system integration between 4kW Nd:YAG laser device and the other systems, on-line real time welding quality monitoring system, perfect safety standards for high power laser, minimization of consumption costs such as arc lamp, protective glass for optic, etc. Laser welding has found a place on Hyundai's production plant in conjunction with the startup of mass production of new sports car, and this production system is the result of a collaboration of its engineers. Outer side sheets are joined to inner side sheets by 122 stitch welds totally. And the length is about 2.4meter.

• 대한산업공학회지
• /
• 제33권2호
• /
• pp.246-253
• /
• 2007

All of the machines in a production line can be classified into bottleneck and non-bottleneck machines. A bottleneck is a resource whose capacity limits the throughput of the whole production facility. This paper addresses a batch sizing problem at the bottleneck machine. Traditionally, most batch sizing decisions have been made based on the EOQ (economic order quantity) model where setup and inventory costs are considered while throughput rate is assumed to be given. However, since batch size affects the capacity of the bottleneck machine, the throughput rate may not be constant. As the batch size increases, the frequency of the setup decreases. The saved setup time can be transferred to processing time, which results in higher throughput. But, the larger batch size may also result in longer lead time and larger WIP inventory level. This paper presents an alternative method to determine batch size at the bottleneck machine in a manufacturing line. A linear search algorithm is introduced to find optimal throughput rate and batch size at the same time. Numerical examples are provided to see how the proposed method works and to investigate the effects of some parameters.

• 복식문화연구
• /
• 제11권4호
• /
• pp.562-577
• /
• 2003

The object of my research is to inquiry into the immediate constituent and techniques of underwear after looking into basic theory of underwear, techniques by materials classified according to the matter of elasticity, and studying flat pattern and draping which are basis of corset and brassiere structure focusing on corsetry among underwears and production techniques. Various papers are referenced for theoretical study and the elements and techniques of underwear are analyzed based on photographical materials. This paper concludes as follows. To make the flat pattern of corset and brassiere fit to the body tightly, design dart two times without any space and draw smaller than the body. To support the breast sufficiently, move the side seam 1¼″ to the front panel and the seam should pass the apex in all occasions. For draping, dart should be added into short of elasticity materials in the past but dart is no use these days since the material today is extremely elastic. It is possible now to show the body line with only the seam or a pattern. In a case of elastic material, set the elastic direction to the grain line and have a draping as pulling from the center to the out side. As production technic, for an hem, put a zigzag stitch in elastic materials with a 10-20% short elastic band, pulling the band. Then make 3/16″-1/4″ short inseam.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2002년도 생물공학의 동향 (X)
• /
• pp.309-312
• /
• 2002

GS system을 이용하여 재조합 EPO를 생산하는 새로운 CHO-K1 세포주를 확립하였으며, 이 세포주를 이용하여 Zn 이온과 Mn 이온이 EPO의 생산에 미치는 영향에 관해서 연구하였다. 형질 전환에 있어서 DNA 3 ${\mu}g$ 올 사용하고, 세포군 선별을 위한 MSX의 농도는 100 11M 을 사용한 경우에만 세포군이 발견되었다. 200 ${\mu}M$의 MSX를 처리한 경우에서는 세포군이 생성되지 않았고, 이는 고농도의 MSX에 의해 세포 생장이 저해되었기 때문이다. 형질 전환된 CHO-K1 세포 배양에 Zn 이온을 첨가한 결과 세포의 생장은 크게 저해되지 않은 반연에, EPO의 생산은 대조구에 비해 40% 이상 증가함을 알 수 있었다. 이러한 결과는 Mn 이온을 처리한 실험에서도 관찰할 수 있었다.

• 한국경영과학회지
• /
• 제27권4호
• /
• pp.41-65
• /
• 2002

We study a multi-component production/inventory system in which individual components are made to meet various demand types. We assume that the demands arrive according to a Poisson process, but there is a fixed probability that a demand requests a particular kit of different components. Each component is produced by a flow line with several stations in which the processing times of each station follow a two-stage Coxian distribution. The production of each component is operated by an independent base-stock policy with blocking. We assume that the time needed to assemble final products follows a general distribution and the capacity of an assembling facility is sufficiently large. The objective of this study is to obtain key performance measures such as the distribution of the number of each orders for each final product and the mean time of fulfilling a customer order. The basic principle of the proposed approximation method is to decompose the original system into a set of subsystems, each subsystem being associated with a flow line. Each subsystem is analyzed in isolation using a Marie's method. An iterative procedure is then used to determine the unknown parameters of each subsystem. Numerical results show that the accuracy of the approximation method is acceptable.

• 한국표면공학회지
• /
• 제53권4호
• /
• pp.138-143
• /
• 2020

In this study, we produced a coil of micro-pattern that can be used for electromagnetic wave absorber, heating material, wireless charging, sensor, antenna, etc. by using electrochemical additive manufacturing method. Currently, it contains research contents for manufacturing a micro pattern coil having practicality through control of process control variables such as applied voltage, distance between electrode, and nozzle injection. Circulation of the electrolyte through the nozzle injection control can significantly contribute to improving the surface characteristics of the coil because of minimizing voltage fluctuations that may occur during the additive manufacturing process. In addition, by applying the pulse method in the application of voltage, the lamination characteristics of the plated body were improved, which showed that the formation of a fine line width plays an important role in the production of a micro pattern coil. By applying the pulse signal to the voltage application, the additive manufacturing characteristics of the produced product were improved, and it was shown that the formation of a fine line width plays an important role in the production of a micro pattern coil.

• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 2001년도 봄 학술발표논문집 Vol.28 No.1 (A)
• /
• pp.643-645
• /
• 2001

B2C 쇼핑몰 시스템의 경우 사용자 인터페이스에 관련된 부분에서만 차이를 보일 뿐 시스템의 기능적인 요구사항과 아키텍처는 유사하며 재사용 가능한 컴포넌트나 COTS 제품을 사용하여 시스템의 많은 부분을 구축할 수 있다. 따라서 쇼핑몰 시스템 구축 시 매번 각 기능들을 설계하고 구현하는 것은 중복 투자를 하는 것과 같다. 이에 본 논문에서는 B2C 소핑몰 시스템의 공통 요구사항을 기반으로 B2C 쇼핑몰 시스템 구축을 위한 프로덕트 라인을 제시한다. 쇼핑몰 시스템 구축에 프로덕트 라인을 적용한다면 중복 투자를 줄이고 미리 준비한 COTS 제품이나 컴포넌트를 사용하여 좋은 품질의 시스템을 빠르게 구축할 수 있다. B2C 쇼핑몰 시스템의 프로덕트 라인을 제시하기 위해 프로덕트 라인의 Core Asset Development 프로세스를 수행하여 쇼핑몰 시스템에 특화된 Product Line Scope, Core Assets, Production Plane을 정의하였다. B2C 쇼핑몰 시스템의 Product Lien Scope는 웹 상에서 물건 판매, 주문 정보 접수, 고객 정보 관리 등의 공통점과 가격의 고정 여부, 검색 등 기타 기능, 공동구매 기능 등의 차이점으로 정의할 수 있다. Core Asset는 B2C 쇼핑몰 시스템의 아키텍처와 시험 및 설계 방법, 재사용 가능한 컴퍼넌트 목록 등이 있다. 마지막으로 Production Plan은 각각의 Core Asset의 부착 프로세스를 연결하여 제시한다. B2C 쇼핑몰 시스템의 Production Plan은 프로젝트 계획, 아키텍처 정체, 사용자 인터페이스 프로토타핑, 컴포넌트 획득, 구현, 통합 및 테스팅, 운영 단계로 구성된다.

• 생약학회지
• /
• 제52권3호
• /
• pp.143-148
• /
• 2021

This study was performed to elucidate the inhibitory effects of Alveopora japonica extract on melanin synthesis by measuring the levels of cell viability, mRNA expression, tyrosinase activity, and melanin production in the B16F10 cell line. The effects of A. japonica extract on tyrosinase-related protein 1 (TYRP1), TYRP2, tyrosinase (TYR), and microphthalmia-associated transcription factor (MITF) mRNA expression levels and melanin content were determined. Quantitative real-time RT-PCR show that A. japonica extract decrease the mRNA expression levels of TYRP1, TYRP2, TYR, and MITF in B16F10 cell line, resulting in lower levels of melanin production compared to α-MSH-treated B16F10 cells. Tyrosinase activity assays reveal that A. japonica extract decrease melanin production in B16F10 cells. These results demonstrate the whitening effects of A. japonica extract on B16F10 cells; thus, A. japonica extract is a potent ingredient for skin whitening. Further research is needed on the mechanism of action of A. japonica extract. Such research will benefit not only cosmetics, but also the health food and medical industries.