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Lycoctonum 속식물성분의 약리학적연구 I (Pharmacological Study of Components of L. pseudo-laeve Nakai.)

  • 김재완
    • 약학회지
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    • 제7권4호
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    • pp.86-91
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    • 1963
  • Crystal A and B were isolated from L. pseudo-leave Nakai and their pharmacological action was observed. Crystal A, in dose of 5 * $10^{-4}$ - 5 * $10^{-5}g/ml$, stimulated excised frog heat but at 2.5 * $10^{-6}$ - 0.5 * $10^{-5}g/ml$ of crystal B, depressant effect was observed. Mild stimulation of rhythmicity of exercised rabbit intestine was produced by 1 * $10^{-4}$ g/ml of crystal A and 2 * $10^{-4}g/ml$ of crystal B. Marked depression of tone was produced by 2 * $10^{-4}g/ml$ of crystal A, antagonizing by pilocarpine. Crystal B(4 * $10^{-4}gm/l)produced$ mild stimulation, followed by depression of tone. No curare-like action on excised frog abdominal muscle was elicited with both substances.

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레이저플라즈마의 제특성의 계측 (Measurement of properties of laser-produced plasmas)

  • 강형부
    • 전기의세계
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    • 제29권2호
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    • pp.118-128
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    • 1980
  • The properties of plasmas produced by high power glass laser were investigated with various methods of diagnostics. Electron temperature was estimated by measurement of soft X-ray, and ion temperature was estimated by measurement of the time-of-flight of ion. The measurement of incident and reflected laser light, and Schlieren and shadowgragh methods were also used. No influence of laser pulse duration on the temperature was observed in the case of durations 2, 4 and 10 nsecs. The effective heating of plasma occurred in about 2 nsec of beginning of incident laser pulse. The experimental results for fast rising laser pulse were discussed and the influence of resetime of laser pulse on the heating of plasma was described. Neutrons produced by irradiating laser beam to solid deuterium target were detected.

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재조합 Lactobacillus paracasei로부터 Bacillus subtilis의 Mannanase 생산과 효소특성 (Production and Properties of a Bacillus subtilis Mannanase from Recombinant Lactobacillus paracasei)

  • 윤기홍
    • 한국미생물·생명공학회지
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    • 제40권3호
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    • pp.186-189
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    • 2012
  • A gene coding for mannanase (manA) from Bacillus subtilis was introduced into a shuttle vector pGK12 between Escherichia coli, B. subtilis and Lactobacillus paracasei. As a result of transferring the resultant plasmid, designated pGK12M3, into three different strains, the manA gene was found to be expressed in L. paracasei as well as in B. subtilis and E. coli. In a 4 L fermentor culture, the production of mannanase by recombinant L. paracasei (pGK12M3) reached a maximum level of 5.4 units/ml in an MRS medium with a fixed pH 6.5. Based on the zymogram of mannanase, it is assumed that mannanase produced by recombinant L. paracasei is not maintained stably with proteolytic degradation. The optimal temperature and thermostability of mannanase produced by recombinant L. paracasei were also found to be different from those of enzymes produced by B. subtilis.

가압형태와 발포제가 분말성형 발포법에 의해 제조된 알루미늄 발포체의 미세구조에 미치는 영향 (The Effect of Pressing Type and Foaming Agent on the Microstructural Characteristic of Al Foam Produced by Powder Compact Processing)

  • 최지웅;김혜성
    • 열처리공학회지
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    • 제34권2호
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    • pp.60-65
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    • 2021
  • In this study, the effect of pressure type and foaming agent on the microstructural change of Al foam produced by powder compact processing was investigated. Better foaming characteristic is easily obtained from extrusion process with strong plastic deformation and preheating than that by uniaxial pressing with preheating. In current powder compact foaming process using TiH2/MgH2 mixture as a foaming agent, a temperature of 670℃ and addition of 30% MgH2 in TiH2 foaming agent was chosen as the most suitable foaming condition. The aluminum (Al) foams with maximum porosity of around 70%, relatively regular pore size and distribution were successfully produced by means of the powder metallurgy method and extrusion process.

Permeability features of concretes produced with aggregates coated with colemanite

  • Bideci, Ozlem Salli;Bideci, Alper;Oymael, Sabit;Gultekin, Ali Haydar;Yildirim, Hasan
    • Computers and Concrete
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    • 제15권5호
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    • pp.833-845
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    • 2015
  • In the world total boron reserve rating, Turkey is taken place on the first rank, meeting the demand of refined mineral and main boron chemicals. Development of the new boron products and production technologies, spreading the using area of the boron are the study topics which must be finically discussed. In this study, with the help of colemanite taken in ratio as (0%, 7.5%, 12.5%, and 17.5%) by being mixed by the cement, surfaces of the pumice aggregates have been covered. Permeability of the samples has been investigated by producing lightweight concrete with 400 dose with the help of aggregates covered with colemanite. For this, the experiments of water absorption, capillary water absorption, depth of penetration of water under pressure and rapid chloride permeability have been performed. In addition, analyses of the thin section of covered and uncovered pumice aggregates and SEM (Scanning Electron Microscope) have been investigated. When the control samples produced with the covered aggregates and concretes produced with colemanite covered aggregates are compared each other, it has been determined that special lightweight concretes whose values of capillary water absorption experiment, depth of penetration of water under pressure experiment and rapid chloride permeability are low can be produced.

Assessment of Potential Probiotic and Starter Properties of Pediococcus spp. Isolated from Turkish-Type Fermented Sausages (Sucuk)

  • Yuksekdag, Z. Nur;Aslim, Belma
    • Journal of Microbiology and Biotechnology
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    • 제20권1호
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    • pp.161-168
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    • 2010
  • In this study, the metabolic activities of five strains of Pediococcus spp., in terms of the quantities they produced of lactic acid, hydrogen peroxide, exopolysaccharides, and proteolytic activity, were determined. Lactic acid levels produced by these strains were found to be in the range of 2.5-5.6 mg/ml. All strains produced hydrogen peroxide. The P. pentosaceus Z13P strain produced the maximum amount (0.25 mg/ml) of proteolytic activity. Exopolysaccharide (EPS) production by the Pediococcus strains during growth in MRS (de Man, Rogosa, and Sharpe) medium was in the range 25-64 mg/l. The susceptibility of 10 different antibiotics against these strains was also tested. All strains were found to be resistant to amoxicillin, gentamicin, and vancomycin. Antimicrobial effects of the Pediococcus spp. on pathogens were also determined by an agar diffusion method. All of the strains were able to inhibit L. monocytogenes. The tolerance of the strains to low pH, their resistance to bile salts of strains, and their abilities to autoaggregate and coaggregate with L. monocytogenes were also evaluated.

Helminthosporium sativum가 생성하는 독소물질에 대한 phytotoxicity 및 Dilution end-Points 측정 방법 개발 (Measurement of Dilution End-Points and Phytotoxicity of Toxic Metabolites Produced by Helminthosporium sativum in Barley, Wheat and Lettuce Roots)

  • 이상선
    • 한국식물병리학회지
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    • 제3권3호
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    • pp.198-202
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    • 1987
  • 밀 보리에서 crown root rot 및 leaf blotch를 이르키는 Helminthosporium sativum의 독소물질을 분리하였다. 이는 독소물질에 대한 어떤 손상도 없이 C-18 column으로 분리되었으며, 실험적으로 가능한 inhibitor를 제거하였다. Lettuce 성장과 독소물질의 농도의 반응에는 semi-log 표에서 비례하였다. 또한 pathogen host인 밀 보리의 뿌리 성장에 사용한 결과 Lettuce의 반응과 동일한 결과를 얻었다. 또한, pathogen host인 밀 보리의 뿌리 성장에 사용한 결과 Lettuce의 반응과 동일한 결과를 얻었다. 독소물질과 식물의 반응을 측정하여 dilution end-points.의 결과로, 직접적으로 독소물질의 농도를 측정하는 방법을 개발하였다. (Y=a log x+b)의 공식과 통계처리로 사용하여 얻은 a와 b의 의미를 토론하였다.

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한국산 고추와 참깨에 발생하는 Alternaria의 종류와 이들의 진균독소 생성능력 (Incidence of Altermaria Species in Red Pepper and Sesame from Korea and Their Ability to Produce Mycotoxins)

  • 이향범;유승헌
    • 한국식물병리학회지
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    • 제11권1호
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    • pp.1-8
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    • 1995
  • Alternaria alternata and A. solani were identified from 130 Alternaria isolates obtained from red pepper fruits, and three species including A. alternata, A. sesami and A. sesamicola were detected from 150 isolates from sesame seeds. Among the 4 Alternaria species, A. alternata was the predominant fungus from both plants, having incidence of 95.4% in red pepper and 56.0% in sesame. Of the total 280 isolates, cultures on autoclaved rice of 75 isolates were tested for toxicity to 21-day-old virgin female rats. Out of 50 isolates of A. alternata, 17 were lethal to rats, inducing congestion and hemorrhage of stomach and intestine and kidney enlargement, and 8 caused lack of weight gain or weight loss. The other 25 isolates of A. alternat and all the isolates of A. sesami, A. sesamicola and A. solani, showed no significant indication of toxicity. Production of mycotoxins in the rice cultures of the above 75 isolates belonging to 4 species was analyzed. Alternaria cultures were extracted with methanol and purified by using solvent partition, thin-layer chromatography, and high performance liquid chromatography. Of the four species tested, all produced alternariol (AOH) and alternariol monomethyl ether (AME), three (A. alternata, A. sesami and A. sesamicola) produced alternuene (ALT) and altertoxin-I (ATX-I), and only A. alternata produced tenuazonic acid (TA). TA was produced by all of the highly toxic (lethal to rats) isolates of A. alternata, but not by any nontoxic isolates.

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돼지에서 분리한 Staphylococcus hyicus subsp hyicus의 protein A (Protein A of Staphylococcus hyicus subsp hyicus isolated from pigs)

  • 김도경;여상건
    • 대한수의학회지
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    • 제30권2호
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    • pp.187-192
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    • 1990
  • 돼지로부터 분리한 Staphylococcus hyicus subsp hyicus 489주의 protein A 존재여부와 함량을 indirect hemagglutination 및 enzyme-linked immunosorbent assay(ELISA)법으로 조사하였다. Indirect hemagglutination text에 의하여 cell-bound protein A 및 extracellular protein A 보유균은 489주 중 각각 87.7% 및 36.0%로 나타났다. ELISA법에 의한 이들 균의 protein A함량 측정에서 전균주의 extracellular protein A는 1ng/ml미만이었으며, cell-bound protein A함량은 대부분의 균주에서 1ng/ml 미만이었고 11주가 25~108ng/ml 수준이었다.

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화학 탐구 맥락에서 중등 과학 교사가 제시한 주장과 증거 분석 (An Analysis of Written Claim and Evidence Produced by Secondary Science Teachers in a Context of Chemistry Inquiry)

  • 김다은;최애란
    • 대한화학회지
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    • 제61권6호
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    • pp.359-368
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    • 2017
  • 본 연구에서는 중등 과학 교사가 화학 탐구 맥락의 결과를 해석하여 제시하는 주장과 증거의 수준이 어떠한지 알아보고자 하였다. 본 연구에 참여한 53명의 과학 교사가 화학 탐구의 결과를 해석하여 제시한 주장과 증거를 분석한 결과 3가지 문항 중에서 여러 개념이 통합된 화학 주제의 경우 1-2수준의 주장과 증거가 상당수 있었고, 화학 교과서에 명시적으로 제시된 화학 개념과 직접 관련된 내용의 경우 3-4수준의 주장과 증거가 많았다. 본 연구의 결과는 과학 탐구의 핵심인 논증, 즉 주장과 증거 제시에 대하여 과학 교사가 적절한 지식과 이해를 함양할 수 있도록 현장 과학 교사 교육이 필요함을 시사한다.