• YAKHAK HOEJI
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• v.44 no.3
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• pp.257-262
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• 2000

The roots of Cynanhum caudatum (Asclepiadaceae) have been used in folk medicine in Japan and China for the prevention and treatment of various geriatric diseases and also as a cardiotonic agent. Constituents of this plant have mainly been examined for glycosides: besides two steroidal alkaloids, gagaminine and gagamine which was firstly isolated by us, more than 35 polyoxypregnane glycosides and aglycones have also been identified. Gagaminine inhibits potently the hepatic aldehyde oxidase activity and lipid peroxidation in vitro. The present work deals with the comparison of antioxidative activities of gagamine, a new pregnane alkaloid, three isolated polyoxypregnanes containing a keto group at C-20 with those of gagaminine, a potent antioxidant, in order to explain the structure-activity relationships. The results of this study further prove that the cinnamoyl group of gagaminine is very important for the inhibition on the aldehyde oxidase activity while the nicotinoyl group is necessary for anti-lipid peroxidation. Besides that, the keto compounds having no ester group at C-12 were found to be more active than the others except gagaminine.

• Journal of the Korean Society of School Health
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• v.27 no.3
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• pp.181-188
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• 2014

Purpose: The purpose of this study was to investigate the influence of physical activity on smoking experience and smoking intensity in Korean high school students. Methods: This cross sectional study used secondary data from the 2013 Adolescent Health Behavior Online Survey. A total of 35,905 high school students were included in the study. Logistic regression was used for analysis of the influence of moderate and vigorous physical activity on smoking experience in a month and smoking intensity. Results: Adolescents with low (Adjusted OR: 1.242, 95% CI: 1.075~1.436) and moderate level (Adjusted OR: 1.094, 95% CI: 1.005~1.189) of moderate physical activity had more smoking experience than reference group, high level of physical activity. Also, smoking experience was more prevalent adolescents with low (Adjusted OR: 1.191, 95% CI: 1.064~1.333) and moderate level (Adjusted OR: 1.134, 95% CI: 1.039~1.237) of vigorous physical activity. Heavy smoking was more prevalent among adolescents who had low level of vigorous physical activity (Adjusted OR: 1.912, 95% CI: 1.347~2.712). Conclusion: Based on the results of this study, physical activity has significant influence on smoking experience and smoking intensity. Therefore, physical activity should be included smoking cessation and smoking prevention program for High School Students.

• Korean Journal of Environmental Biology
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• v.30 no.3
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• pp.157-163
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• 2012

Oxidative stress has been reported to be one of causes of neuritis. This study examined antioxidative activities of methanol extracts of six amphibian species known to be medicinal animals (Rana catesbeiana, R. coreana, R. rugosa, R. dybowskii, R. nigromaculata, and Hyla japonica) and investigated their effects of inhibiting nitric oxide (NO) production and cytotoxicity on the murine macrophage RAW264.7 cells. As inflammation is closely associated with reactive oxygen species, assays on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, xanthine oxidase inhibitory activity, superoxide anion radical scavenging activity and NO scavenging activity of the extracts of the six species were performed to investigate their antioxidative activity. The results obtained were as follows; All extracts showed antioxidative activity, and the activity of R. dybowskii was the highest in comparison among those. Anti-inflammatory effects of the extracts were also examined, the five extracts except that of R. rugosa did not show cytotoxicity for RAW264.7 cells at the maximal concentration ($1,000{\mu}g\;mL^{-1}$). Selectivity index, meaning NO scavenging activity compared to cytotoxicity, showed the highest level in the extract of R. dybowskii. These results will be very useful basic data for future studies on prevention and treatment of human diseases to understand the biological roles of amphibian extracts throughout the antioxidative or anti-inflammatory pathways.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.6
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• pp.895-899
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• 2002

Lysozyme activity in buffalo milk in relation to the period of lactation, parity of animal, weather conditions and udder infections was studied. Effect of storage and heat processing of milk on lysozyme activity was determined. Lysozyme activity was higher in buffalo milk than in cow milk. Buffalo colostrum showed lysozyme activity 5 times of that in mature milk. Lysozyme activity in buffalo milk was not influenced by the parity of animal and the stage of lactation, however, it increased during extreme whether conditions (winter and summer). Lysozyme in both cow and buffalo milk exhibited maximum activity at pH 7.4. Buffalo milk lysozyme was fully stable while the cow milk lysozyme was partly inactivated by pasteurization (low temperature-long time as well as high temperature-short time treatments). Lysozyme in buffalo milk was more stable than in cow milk during storage and heat treatment. A 10 to 50-fold increase in milk lysozyme activity was observed in mastitic cows. An assay of lysozyme activity in milk can be used to diagnose mastitis in cattle but not in buffaloes. Some buffaloes exhibited 1000 fold greater lysozyme activity and moderately raised somatic cell count in milk, but there was no sign of mastitis in these animals. A possible role of milk lysozyme in prevention of mastitis in buffaloes is discussed.

• Osong Public Health and Research Perspectives
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• v.9 no.6
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• pp.348-353
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• 2018

Objectives: This study was conducted to determine whether essential oils had anti-influenza A/WS/33 virus activity and whether there were specific compounds associated with this activity. Methods: There were 63 essential oils evaluated for anti-influenza (A/WS/33 virus) activity using a cytopathic effect reduction method. The chemical composition of the anti-influenza essential oils was phytochemically analyzed by gas chromatography-mass spectrometry. Results: The antiviral assays demonstrated that 11 of the 62 essential oils ($100{\mu}g/mL$) possessed anti-influenza activity, reducing visible cytopathic effects of influenza A/WS/33 virus activity by > 30%. Furthermore, marjoram, clary sage and anise oils exhibited anti-influenza A/WS/33 virus activity of > 52.8%. However, oseltamivir (the anti-influenza A and B drug), showed cytotoxicity at the same concentration ($100{\mu}g/mL$) as the essential oils. The chemical composition detected by GC-MS analysis, differed amongst the 3 most potent anti-viral essential oils (marjoram, clary sage and anise oils) except for linalool, which was detected in all 3 essential oils. Conclusion: This study demonstrated anti-influenza activity in 11 essential oils tested, with marjoram, clary sage and anise essential oils being the most effective at reducing visible cytopathic effects of the A/WS/33 virus. All 3 oils contained linalool, suggesting that this may have anti-influenza activity. Further investigation is needed to characterize the antiviral activity of linalool against influenza A/WS/33 virus.

• Journal of Microbiology and Biotechnology
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• v.24 no.5
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• pp.704-713
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• 2014

The highly pathogenic avian influenza A (HPAI) viruses of the H5N1 subtype infect poultry and have also been spreading to humans. Although new antiviral drugs and vaccinations can be effective, rapid detection would be more efficient to control the outbreak of infections. In this study, a phage-display library was applied to select antibody fragments for HPAI strain A/Hubei/1/2010. As a result, three clones were selected and sequenced. A hemagglutinin inhibition assay of the three scFvs revealed that none exhibited hemagglutination inhibition activity towards the H5N1 virus, yet they showed a higher binding affinity for several HPAI H5N1 strains compared with other influenza viruses. An ELISA confirmed that the HA protein was the target of the scFvs, and the results of a protein structure simulation showed that all the selected scFvs bound to the HA2 subunit of the HA protein. In conclusion, the three selected scFVs could be useful for developing a specific detection tool for the surveillance of HPAI epidemic strains.

• The Journal of the Korea Contents Association
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• v.18 no.4
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• pp.223-233
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• 2018

This study examined the factors influencing the suicide prevention gatekeeper participation of 299 grassroots organizational leaders in Gangwon province. For this purpose, participation of activities of suicide prevention gatekeeper was classified into performance intention and intervention level. Performed hierarchical multiple regression analysis by setting participation motives which showed a significant correlation with participation of suicide prevention gatekeeper as independent variables. As a result of the analysis, participation motives affecting performance intention were a sense of mission as a leader, benefits or rewards for activities, the meaning and purpose of suicide prevention, government policy. And participation motives affecting intervention level were invitation of public officials, the meaning and purpose of suicide prevention. Based on the results of this study, suggested implications for the participation of suicide prevention activities in community grassroots organizations and suggestions for future activation.

• Journal of the Korea Safety Management & Science
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• v.11 no.3
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• pp.27-32
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• 2009

Human physical activity to lead daily life is performed by a variety of muscular activity strategies. These strategies can be influenced by characteristics, difficulty and muscle fatigue of work. Therefore this study examines muscular strengths (grip and back strength) of workers engaged in electronics industry in Korea, compares them to mean value of muscular strength by sex and age, accumulates related data and then aims to develop improvement methods of working environment and prevention/management program.

• Electronics and Telecommunications Trends
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• v.33 no.1
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• pp.101-110
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• 2018

Based on the main technologies of the 4th Industrial Revolution, industries including the smart home, transportation, agriculture, factory, energy, and medical care industries are rapidly developing. Disaster management technologies and services based on state-of-the-art convergence technologies are being widely applied for the purposes of public safety. State-of-the-art scientific technologies including the Internet of Things (IoT) are expected to offer alternative solutions to pending issues of disaster and safety. Particularly in disaster management, a "prevention activity"to avoid and control disasters in advance is essential, and thus disaster prevention and safety monitoring technologies based on hyper-connected intelligence are fundamental for society during the 4th Industrial Revolution. IoT technologies are being actively applied and utilized in various fields to prevent social and natural disasters. In this article, we introduce the development trends of disaster prevention and safety monitoring technologies based on IoT technologies.

• The Journal of the Korean dental association
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• v.50 no.9
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• pp.552-557
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• 2012

Bamboo salt is a special processed salt by Korean traditional recipe. Recent study results showed that bamboo salt or bamboo salt with some other materials like herbal extracts have the anti-microbial activity, inhibition effects of dental plaque and gingival inflammation. Bamboo salt also showed anti-cariogenic effects; remineralization and acid resistance. Compare to fluoride toothpaste, bomboo salt toothpaste with fluoride showed the more effective remineralization on inner part of the early dental caries lesion. It increased the surface hardness and decreased lesion depth of early dental caries lesion. Thus, it is suggested thai bamboo salt could be used as a anti-microbial, anti-plaque, anti-inflammatory and anti-cariogenic material for oral disease prevention. Especially, bamboo salt dentifrice with fluoride can be recommanded as a useful remineralizing agent.