Various cryoprotective agents (CPA) were tested to establish the best conditions for the cryopreservation of sperm from black porgy Acanthopagrus schlegeli acclimated and raised in freshwater (BFW). Survival rates of frozen/thawed sperm from BFW were higher in the order of dimethy sulfoxide (DMSO), glycerol, ethylene glycol (EG) and methanol. Sperm motility was higher in the order of glycerol, DMSO, EG and methanol. These effects were the same in thawed sperm from black porgy raised in seawater (BSW). Thus, optimum CPA for sperm cryopreservation of BFW and BSW were DMSO and glycerol where the highest survival rates and sperm motility were found at the concentration of 10%. In particular, the survival rates and motility of thawed sperm from BFW and BSW after cryopreservation using 10% DMSO were better than when cryopreserved using 10% glycerol. On the other hand, for the thawed sperm from both BFW and BSW, the longer the preservation period was, the lower the survival rates and sperm motility were. Notably, the higher the concentration of CPA was, the lower the survival rates and sperm motility were.
Lee, Dong-Sun;Koo, Young-Jo;Shin, Dong-Hwa;Thorpe, Roy H.
Korean Journal of Food Science and Technology
/
v.13
no.3
/
pp.219-226
/
1981
To enable off-season peach canning, peaches were halved and processed into solid packs. Following variables were compared in relation to storage stability of solid packs. First as pretreatment-dipping in citric and ascorbic solution after lye peeling; second as pasturization in pouch and hot fill process; third as packaging method-2 ply(PET/PE) and 3 ply(PET/Al/PE) plastic film pouch and metal container; fourth as storage condition $-20^{\circ}C\;and\;-5^{\circ}C$. After 25 weeks storage test, all peaches in different packs were processed into canned peaches just like commercial products, and then quality was organoleptically evaluated from the viewpoint of processing and storage variable of solid packs. The results are follows; 1. Ascorbic acid dipping in 3 ply pouch and citric acid dipping in 2 ply pouch had better quality retention effect than any other pretreatments in respective packing. 2. Pasteurization method gave great effect on the color and texture of the solid pack during storage, but not on the quality of the final canned peach. 3. Can and 3 ply packing were better than 2 ply pouch in storage of solid pack and quality of final canned peach. 4. The $5^{\circ}C$ storage gave better quality than $20^{\circ}C$.
The variety and density of entomopathogenic nematodes from the polluted soils of heavy metals were examined. In order to investigate the pollution of heavy metals in soil, 300 sites in kyungsangbuk-do were collected from March to October in 2001. We measured the contents of seven heavy metal elements (Cd, Cu, As, Hg, Pb, Cr^{6+}$, CN) and than cheesed soil of 25 sites with high concentration of heavy metals. The seven strains of nematodes were isolated from seven samples by silkworm host (Bombyx mori mori) and white trap. Isolated nematodes are composed of two families, one order. The members of Rhabditida were isolated in the soil with mean Cd content of 0.870 ppm. And they were isolated in the soil samples with As content less than 0.745 ppm. However they were isolated regardless of concentration of Cu and Pb. The members of Cylindrocorpidae were isolated in the soil samples with Cr^{6+}$ content less than 0.05 ppm. Any entomopathogenic nematode was not detected in the CN polluted soil. Isolated nematodes successfully cultured on the silkworm host and were confirmed the pathogenicity, multiplicity, and tolerance against various condition of preservation. Which proved its potential usefulness as biological agent.
This study examined visitors' perceptions and attitudes about establishing cable car in Mudeungsan Provincial Park (MPP). Data were collected from 401 respondents by a field questionnaire survey in MPP during the September of 2010. 61.7% of respondents opposed to establishing cable car in MPP while 20.6 percent supported it. Respondents showed high level of belief to possible consequences that establishing cable car will result in 'deterioration of ecosystem and natural landscape', 'crowding problem by increased number of visitors', 'disappearance of naturalness or previous nature experiences in MPP'. Respondents, however, presented low belief to 'positive effects on increasing economic income and local economic growth by attracting tourists'. 32.4 percent of the survey respondents evaluated the present MPP as a place of 'Nature Preservation Area (NPA)' and 10.5% as 'Developed Park Area (DPA)'. 48.8% of respondents answered that MPP should be managed as 'NPA' with only 2.5% as DPA. Respondents' socio-demographic characteristics (i.e., gender, age, education level, length of residence in Gwang-ju city), use patterns (number of visits to MPP or to Jungbong and surrounding areas), and normative place condition of MPP showed relationship with the overall support rates about establishing cable car. The results and their implications were discussed.
Kim, Kwang-Mo;Park, Jung-Hwan;Park, Byoung-Soo;Son, Dong-Won;Park, Joo-Saeng;Kim, Wun-Sub;Kim, Byoung-Nam;Shim, Sang-Ro
Journal of the Korean Wood Science and Technology
/
v.37
no.4
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pp.330-339
/
2009
The material properties of Cedar (Cryptomeria japonica) were evaluated according to heat treatment conditions. The special focus was made on the color control of cedar wood by heat treatment. The difference of color between sapwood and heartwood could be reduced by heat treatment at a temperature above $170^{\circ}C$. Long heating time was more effective in reducing the difference. The Equilibrium Moisture Content (EMC) of heat-treated wood was as low as 50 percent. The result obviously indicates that heat-treated wood is more dimensionally stable in the change of moisture condition than the control. The heat-treated wood was also effective in increasing the durability against wood rotting fungi. However, more study is required to develop heat treatment as an environmentally-friendly technology for wood preservation without chemical. The mechanical properties of heat-treated wood showed relatively higher performance than the control in general. Meanwhile the dramatic decrease in impact bending stress due to the loss of ductility may limit uses of heat-treated wood in certain cases. There were no significant changes in microscopic structure which may cause changes in mechanical properties. Further study on the chemical analysis of heat-treated wood is needed to scrutinize the causes of changes of material properties.
Fresh jujubes (Zizyphus jujuba Miller) of whitish green and red ripe maturities were stored at 5 different temperatures, and quality changes through the storage were measured to find an optimal storage condition. Respiration rates and their temperature dependences for both maturities were not different from each other, which suggested non-climacteric pattern of postharvest respiration. Red ripe fruits showed heavier weight and higher content in soluble solid and ascrobic acid compared with whitish green mature fruits. Through the storage of jujubes in perforated packages tissue softening and decay were main visual quality deteriorations with the former preceding the latter. The whitish green mature jujubes showed slower rate of quality changes in softening and decay than red ripe ones, and are thus more suitable for long term storage. In the storage, the whitish green fruits changed into red color, and showed increase in soluble solid and decrease in acidity and ascorbic acid content. Storing the jujubes at $-2^{\circ}C$ resulted in symptoms of chilling injury, and storing at higher temperatures above $0^{\circ}C$ accelerated the decay and softening. $0^{\circ}C$ was found to be optimal temperature for long term storage, where jujube had the lowest rate of quality changes without chilling injury. Even at optimal temperature of $0^{\circ}C$, however, storage life retaining freshness was only around 40 days which is not enough.
In the 1990s, when the viewpoint that an ecosystem is a single network within a specific region was adopted, the preservation and management of natural ecosystems was proposed. With regard to Northeast Asia, the expansion of trans-boundary pollution due to rapid development and the swift destruction of the natural environment emphasize the necessity for environmental cooperation. The Northeast Asia region made up of South Korea, North Korea, three northeastern prefectures in China, the Russian far-east, and parts of Mongolia were selected to be analyzed for an ecological network. The significance of this study lies in the development of a methodology for building a Northeast Asian ecological network through the use of satellite images. Regarding the methods of analysis, stable habitats for four priority species were selected to be performed using overlay analysis. The result of the analysis of the ecological networks in the whole Northeast Asia region showed that there were key areas partly dispersed in the Korean Peninsula, but whether the key areas would be maintained in the long term is unknown. As for China, key areas were concentrated in the border areas around the Tumen River and in parts of the three northeastern prefectures. Russia had wide-ranging areas that could function as stable habitats for most species. As a result of the actual conditions of the ecological networks, most of the Northeast Asia region, including the Korean Peninsula, was in poor condition, requiring appropriate measures and their operation as soon as possible. Also, it was revealed that further investigation and research was necessary for border areas that were identified to be key areas.
Journal of the Korean Society of Food Science and Nutrition
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v.12
no.2
/
pp.62-65
/
1983
As a new practical method for preserving freshness of fish, partially frozen storage has been reported to be useful in terms of K-value, TBA value, sensory evaluation etc. In order to develop a storage procedure to be used in place of cooled or frozen storage for the preservation of precooked fish food, partial freezing for up to two to three weeks was examined using baked mackerel. The criteria for evaluation were made according to the changes in volatile basic nitrogen (VBN), thiobarbituric acid (TBA) value, viable bacterial number, pH, color difference and sensory evaluation in fish muscle. The changes in TBA value of baked muscle during storage at $-3^{\circ}C$ differed slightly from those obserbed during cooled ($5^{\circ}C$) and frozen storage ($-20^{\circ}C$). Partial frozen storage ($-3^{\circ}C$) was effective in prolong an induction period of lipid oxidation during early storage. VBN of baked muscle tends to increase slowly while pH value was decrease during storage and there was no observed significant differences among three different storage condition. Viable bacterial number of the baked mackerel muscle stored at $-3^{\circ}C$ showed significantly less than that stored at $5^{\circ}C$, and similar to that stored at $-20^{\circ}C$ (the levels of $10^2/g$). Judging from the results of sensory evaluation and experimental data, partial frozen storage at $-3^{\circ}C$ seems to be effective as means of short-period preserving baked mackerel.
This thesis is a try for examining the historical characteristics of Constiutional medicine. For this purpose, I have examined their theory, and made a comparison carefully. Through this study, I have obtained several results as following. Both Chang and Lee lived in the time of change, which was unstable and uncertain. Under the condition, they both rejected following the existing trends of learned circles, and developed new thoughts and clinical techniques. They rejected superstitious beliefs and fatalism, and conducted the pursuit of experimental knowledge and rationale idea. Clinical experience was the very base of their study. They both criticized the bad habits of abuse of tonifing medication and health seeking. Especially Lee regarded control of emotion and regulation of a way of life as the best way for preservation of one's health. Chang regarded pathogenic factors as the ultimate factor of diseases, and strived for eliminating pathogenic factors, but L brought to a conclusion that the final factor of diseases was what is called "heart" itself, and emotional changes were the most essential causes of disease. It can be said that the pathogenesis insisted by Chang can be called The insistence that pathologic factors are the very etiology of all the disease (邪氣致病論), or all the diseases result from pathologic factors. And his whole remedy can be summarized as following, A study on the method of eliminating pathogenic factors. But the purpose of Constitutional medicine is to correct imbalance intrinsic to one's internal organs. In this aspect, Constitutional medicine is a "regulatory medicine". Depending on the classification of six vital substances, Chang classified all disease into six categories. These were based on symptoms and sings represented. While classification of diseases made by Lee was likely to rely upon constitutional characteristics. Chang thought that the three remedies made up of perspiration, purgation, vomiting were the most efficient way of eliminating pathogenic factors. Lee, however, thought those weren't methods of eliminating pathogenic factors but the best ways restoring one's self-regulation power. Chang thought that all the febrile disease essentially has a tendency in properties to belong to "heat", but Lee pointed out that pathologic processes are variable in accordance with constitutional features. They both regarded pathogenesis of diabetes as fire. That is to say, fire is the most essential factor of diabetes. And there are many risk factors such as inappropriate foods, drugs, climate, etc., but Lee thought what is most important is heart. Putting all accounts together, medical characteristics of Chang are similar to those of T aiyinjen and Shaoyangjen, and have no relation to those of those of Shaoyinjen. Therefore we can conclude that Chang understood pathologic processes of disease of T aiyinjen and Shaoyangjen, whether he knew about constitutional features or not.
Groundwater in small islands is used as main water resource but the overuse of groundwater may cause seawater intrusion and temperature decrease in geothermal wells. This study aimed to characterize the hydrogeology of Maeum-ri area in Seokmo Island of Ganghwagun using long-term monitoring at groundwater wells and geothermal wells. In the monitoring period seasonal water level change, consistent drop or increase of water levels are not detected. The groundwater temperature about 10m below ground surface shows year cycle variation having two to five months difference with ambient temperature cycle. The storativity was calculated by tidal method. The storativity estimated by adapting tidal efficiency factor showed some larger values than that by using tidal time lag. The result suggested that the tidal method assuming several assumptions on aquifer condition may produce broad ranges but the calculated ranges at this application are reasonable. The similar shape of groundwater level change and tidal effects was observed at several wells clustered east-south-east direction which may implicate the distribution of vertical fracture system strongly related with groundwater flow channels. The applied methodology and study results will bc valuable to evaluate optimal pumping rate for the preservation of groundwater resources, and to manage geothermal development.
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