• Journal of Chest Surgery
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• 제17권2호
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• pp.257-262
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• 1984

Acute purulent pericarditis, though not common in incidence after introduction of antibiotics, is still potentially life treating isease. Since 1971, we have experienced 14 cases of acute purulent pericarditis with successful treatment. Among these 14 cases, 9 cases were male and they had high occurrence on their third to fifth decades in age distribution. Isolation of causative organisms were obtained in 11 cases through the bacterial culture of infectious source which was mainly pericardial effusion or blood, and the most frequently recovered organism was the staphylococcus aureus. Pre-existing inflammatory disease preceding to pericarditis, named as antecedent disease, were proved in 12 cases, and among which contiguous extension from the intrathoracic infection such as pneumonia or empyema accounted for the majority of antecedent disease. Pericardiocentesis with administration of antibiotics were tried in all cases, but result in recovery in 1 patient only. Remaining 13 cases had persistent picture of pericarditis and necessitated surgical drainage procedure. Ten of these 13 cases were underwent the open pericardial window using a mode of anterior approach in 4 and subxiphoid approach in 6 cases respectively. Two cases of subxiphoid group were reoperated by the anterior interphrenic pericardiectomy, due to insufficient drain of too thick effusion. In remaining 3 cases, anterior interphrenic pericardiectomy was performed initially because of purulent effusion already changed into fibrinopurulent peel with thickened pericardium. Through the experience of this series, we recommended that pericardiectomy should not be reluctant in purulent pericarditis as a initial surgical procedure for advantage of complete removal of infected space and avoidance of late constrictive pericarditis.

• 한국보건간호학회지
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• 제30권1호
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• pp.70-82
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• 2016

Purpose: The purpose of this study was to develop a multicultural education program for nursing students and to examine its effects on multicultural sensitivity and efficacy. Methods: A research design for this study was a non-equivalent control group, non-synchronized pre-post test. The experimental group (n=34) participated in a 13-week multicultural education program while the control group (n=35) attended regular classes having no relation with multiculture for the same period as that of the experimental group. Multicultural sensitivity and efficacy were measured before and after the program. Data were collected using self-administered questionnaires and analyzed using the PASW 18.0 program. Results: Total scores for multicultural sensitivity and efficacy were significantly increased in the experimental group compared with the control group after the program. The subcategories of multicultural sensitivity; 'respect for cultural differences', 'interaction enjoyment' and multicultural efficacy; 'general functions', 'considering multicultural families' were increased respectively, whereas others were not changed. Conclusion: Based on these results, it can be suggested that a 13-week multicultural education program is effective for improving nursing students' level of multicultural sensitivity and efficacy.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2009년도 추계학술발표논문집
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• pp.937-941
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• 2009

현재 가장 일반적으로 사용되는 미생물 배양 용기인 petri dish를 통해 제조된 미생물 배양 배지의 보관은 냉장과 실온 보관 시 수분 증발에 대한 보호 효과가 극히 낮아 한 달 이상으로 배지 품질을 유지하기 어려운 단점이 있다. 따라서 본 연구는 petri dish를 이용하여 미생물 배양 배지를 제조할 때 glycerol 등 7종의 보습성분을 첨가하여 이에 따른 미생물 배양 배지의 수분 증발 억제 효과와 표준균주별 증식반응을 비교, 분석한 것으로 결과적으로 sodium lactate를 제외한 6종의 보습성분이 배지의 수분 증발을 억제하는 효과가 있으며 이들에 대한 표준균주별 증식반응에서도 양성의 결과를 보여주었다. 앞으로 본 연구의 결과는 즉시 사용 배지(PPM, Pre-Plated Media)의 유통기한 연장 효과 및 품질향상에 유용한 자료로 활용될 수 있을 것으로 기대된다.

• Journal of Chest Surgery
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• 제28권8호
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• pp.731-741
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• 1995

Cardiac valve allografts have been used as replacements for diseased valves and right ventricular outflow tract reconstruction, the long term follow-up of which has been reported satisfactory. For a good long-term result, it is essential that the allograft be viable at implantation. In this study, we aimed at preparing the cardiac valve allografts aseptically, preserving them at cold- and cryo-conditions, and testing the viability of the allografts after preservation by four methods. We tested the viability of the cardiac valve allografts preserved in cold refrigerated state[4$^{\circ}$C in nutrient media & in liquid nitrogen tank[cryopreservation under -149$^{\circ}$C for pre-planned time periods. The testing methods were 1 glucose utility test 2 tissue culture 3 thymidine uptake test and 4 histologic evidence by light microscopy. We observed no differences in the viability between cold- & cryo-groups and similar results among the methods for testing the viability. In conclusion, there was no difference in the viability between cold- and cryopreserved-allografts at least for 14 days of preservation. And glucose utility test and thymidine uptake test were satisfactory in the evaluation of the allograft viability, since they were easy and rapid with relatively quantitative results.

• 한국미생물·생명공학회지
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• 제32권3호
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• pp.238-242
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• 2004

D. bryoniae를 원인균으로 하는 참외 만고병에 대해서 항만고병 활성물질을 생산하는 미생물을 방선균으로부터 선별 분리한 후 배양액으로부터 항균활성물질의 규명을 시도하였다. 항진균 물질과 같은 이차대사산물의 생산에 증가시키는 $K_2$$HPO_4$와 칼슘이온이 포함된 GSS배지에서 방선균 SKM338 균주를 180 rpm, $30^{\circ}C$, 5일 동안 배양하여 얻어진 배양 상등액으로부터 물리화학적인 방법으로 항진균 활성이 있는 물질을 분리 정제한 결과 참외의 만고병에 대한 생물농약으로 개발 가능한 방선균 유래의 항진균성 물질은 NMR, IR, UV 및 Mass spectral data 분석 등을 통해 polyene macrolide계에 속하는 항생물질인 Flavofungin, Fungichromin, Filipins로 밝혀졌으며 이들의 응용을 기대해 본다.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1161-1168
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• 2009

Ethanol production by the simultaneous saccharification and fermentation (SSF) of low-value rice wine cake (RWC) without cooking was investigated. RWC is the filtered solid waste of fermented rice wine mash and contains 53% raw starch. For the SSF, the RWC slurry was mixed with the raw-starch-digesting enzyme of Rhizopus sp. and yeast, where the yeast strain was selected from 300 strains and identified as Saccharomyces cerevisiae KV25. The highest efficiency (94%) of ethanol production was achieved when the uncooked RWC slurry contained 23.03% starch. The optimal SSF conditions were determined as 1.125 units of the raw-starch-digesting enzyme per gram of RWC, a fermentation temperature of $30^{\circ}C$, slurry pH of 4.5, 36-h-old seeding culture, initial yeast cell number of $2{\times}10^7$ per ml of slurry, 17 mM of urea as the nitrogen additive, 0.25 mM of $Cu^{2+}$ as the metal ion additive, and a fermentation time of 90 h. Under these optimal conditions, the ethanol production resulting from the SSF of the uncooked RWC slurry was improved to 16.8% (v/v) from 15.1% (v/v) of pre-optimization.

• 한국항공운항학회지
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• 제18권4호
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• pp.80-85
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• 2010

The study of the safety perceptions between two groups, trained and untrained student pilots were compared as pre-studies of that how the safety perceptions of the flight instructors affect that of the student pilots. As a results, the factors of the communication and the safety procedures shows higher values on the one-year trained group than the other because the trained students get used to the safety procedures which are necessary to the practical training. In reliability for the flight instructor, the factors of two groups show the high tendency without regard to groups. Despite of the lack of the specific research, the result implies that the student pilots are influenced by the safety perceptions of the flight instructors. In addition, the factors of the accident report were investigated as that the trained group has lower mean, however the factors of the receiving penalties of the trained group were higher than the other. These results imply that the trained group feels concern for the penalties and the punishments by reporting the accidents in spite of amounts of the training.

• 한국균학회지
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• 제8권3호
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• pp.159-166
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• 1980

검정곰팡이(Aspergillus niger)의 동조적(同調的) 분화(分化)를 시행(施行)하면서 acid-phenol 용성(溶性)인 세포단백질(細包蛋白質)을 추출(抽出)하여 포자형성(胞子形成) 전후(前後)의 단백상(蛋白像)의 변화(變化)를 polyacrylamide gel 전기영동법(電氣泳動法)으로 추구(追究)하였다. 결과(結果)에서 1종(種)의 단백질(蛋白質)(분자량(分子量) 약(約) 27,000 정도(程度))이 포자형성기(胞子形成期)에 신생(新生)함을 알았다. 포자(胞子)의 발아시(發芽時)에는 18개의 단백질(蛋白質)밴드가 있으며 포자형성시(胞子形成時)에는 19개가 있었다. Amido black 색소(色素)의 염색도(染色度)는 분화시기(分化時期)에 따라서 각(各) 밴드마다 각각 많이 달랐다.

• Mycobiology
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• 제38권4호
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• pp.286-294
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• 2010

Epicoccum purpurascens stain 5615 AUMC was investigated for its biocontrol activity against root rot disease caused by Pythium irregulare. E. purpurascens greenhouse pathogenicity tests using three leguminous plants indicated that the fungus was nonpathogenic under the test conditions. The germination rate of the three species of legume seeds treated with a E. purpurascens homogenate increased significantly compared with the seeds infested with P. irregulare. No root rot symptoms were observed on seeds treated with E. purpurascens, and seedlings appeared more vigorous when compared with the non-treated control. A significant increase in seedling growth parameters (seedling length and fresh and dry weights) was observed in seedlings treated with E. purpurascens compared to pathogen-treated seedlings. Pre-treating the seeds with the bioagent fungus was more efficient for protecting seeds against the root rot disease caused by P. irregulare than waiting for disease dispersal before intervention. To determine whether E. purpurascens produced known anti-fungal compounds, an acetone extract of the fungus was analyzed by gas chromatography mass spectrometry. The extract revealed a high percentage of the cinnamic acid derivative (trimethylsiloxy) cinnamic acid methyl ester. The E. purpurascens isolate grew more rapidly than the P. irregulare pathogen in a dual culture on potato dextrose agar nutrient medium, although the two fungi grew similarly when cultured separately. This result may indicate antagonism via antibiosis or competition.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8211-8214
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• 2016

Background: Having a conscience is one of the main pre-requisite of providing nursing care. The knowledge regarding levels of conscience among nurses in eastern countries is limited. So, the purpose of this study was to examine the level of conscience and its related factors among Iranian oncology nurses. Materials and Methods: This descriptive-correlational study was conducted in 3 hospitals in Tabriz, Iran. Overall, 68 nurses were selected using a non-probability sampling method. The perceptions of conscience questionnaire was used to identify the levels of conscience among nurses. The data were analyzed using SPSS version 13.0. Results: The mean nurses' level of conscience scores was 72.7. In the authority and asset sub-scales nurses acquired higher scores. The mean of nurses' scores in burden and depending on culture sub-scales were the least. Also, there were no statistical relationship between some demographic characteristics of participants and their total score on the perceptions of conscience questionnaire. Conclusions: According to study findings Iranian nurses had high levels of conscience. However, understanding all the factors that affect nurses' perception of conscience requires further studies.