• 한국미생물·생명공학회지
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• 제18권4호
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• pp.331-337
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• 1990

토양에서 새로히 분리한 곰팡이를 배양하고 그의 배양액 중에 함암활성을 갖는 물질을 screening한 결과 시험균 B.subtilis에 대해 항균활성을 갖고 glutathoine에 의해 항균활성이 길항되는 물질을 생산하는 B-1123 균주를 얻었다. 생산균주의 형태학적, 배양학적 성상의 관찰 결과 B-1123 균주는 Aspergillus terreus로 동정되었다. 대두분을 질소원으로 한 배지에서 $30^{\circ}C$, 5일간 진탕배양하고 배양여액 중의 활성물질을 ethyl acetate로추출하고 silica gel column chromatography에 의해 정제 하여 연황색 판상결정을 얻었으며 B-1123 물질이라 명명했다.

• 한국연초학회지
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• 제17권2호
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• pp.103-108
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• 1995

Antibiotic and physico-chemical properties of an active compound from actinomycetes isolate G-37, of which the culture filtrate had an inhibitory effect against tobacco mosaic virus(W) infection, were examined. The active compound, which was purified by ethylacetate extraction, silica gel column chromatography, preparative thin layer chromatography, and high performance liquid chromatography, showed strong antibacterial activities especially against Gram-positive bacteria including Bacillus subtillis, Sarcina lutea and Staphylococcus aureus. From the IH-NMR, FAB/RfS, UV spectral data, and physicochemical properties, the active compound of G-37 appears to belong to a peptide antibiotic group. Among the known peptide antibiotics in the antibiotic group, No. 280, A-30912, and Taitomycin showed molecular weights and ultra violet spectrum similar to those of the active compound from G-37, but was not identical to the compound, which suggests that it may be a new peptide antibiotics.

• 생약학회지
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• 제4권4호
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• pp.167-172
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• 1973

Five crystalline substances, which are positive to$L_{IEBERMAN}-B_{URCHARD}$ reaction, were isolated from the unsaponifiable fraction of the fresh leaves of Betula latifolia $K_{OMAROV}$ (Betulaceae)by silica gel column chromatographic purification. Compound $A\;(C_{29}H_{50}O,\;mp\;136^{\circ}), \;B\;(C_{30}H_{52}O_3,\;mp\;165^{\circ}), \;C\;(C_{30}H_{52}O_4,\;mp\;237^{\circ}), \;D\;(C_{30}H_{52}O_3,\;mp\;196^{\circ})\;and\; E\;(C_{30}H_{52}O_4,\;mp\;121^{\circ})$ were isolated. Compound B was characterized as a new tetracyclic triterpenoid. Compounds A, C and D were identified as ${\beta}-sitosterol$, betulatriterpene C, and betulafolienetriol, respectively.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2001년도 가을 학술발표회 논문집
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• pp.143-150
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• 2001

In this paper, the effects of vehicle loads on flat slab system are investigated based on the previous studies on beam-girder parking structural system. The influence surfaces of flat slab for typical design section are determined for the purpose of obtaining member forces under vehicle loads. In addition, the equivalent vehicle load factors for flat slab parking structures are suggested using neural network. It has been found that vehicle load effects of flat slab system are dominant for the center positive moment in both column and middle strip, as like beam-girder parking structural system.

• Journal of Welding and Joining
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• 제9권4호
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• pp.69-74
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• 1991

The Plasma Arc Cutting Method using high density and hight temperature beam is well applicable to the cutting of the nonferrous metal (Al alloy ) and stainless steel which are unable to be cut by the use of the oxy-fuel gas. This study focalizes on the cutting phenomena of the plate of (mm) thickness, since the cutting phenomena of thick plates have been rather thoroughly studied. In this study the cutting groove, adhesive phenomena of dross, surface roughness were measured according to the variation of cutting speed and compared with the case of mild steel plates. The result showed that the kerf width variation of Al alloy was similar to the case of mild steel, while that of the stainless steel differed from the mild steel. In the adhesive phenomena of dross, 6(mm) thick plates of Al alloy showed a difference from those of thick plates, but the stainless steel was similar to thick plates. The surface roughness variation of Al alloy wias minimum at 67 cm/min, while that of stainless steel was at 30cm/min.

• Ocean and Polar Research
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• 제42권1호
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• pp.15-20
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• 2020

The marine sponge Hyrtios sp. collected in the Philippines was extracted and partitioned. The resulting organic layer was purified by C₁₈ reversed-phase column chromatography and HPLC to achieve the separation of nine scalarane-type sesterterpenes, including one new compound with eight known scalarane analogs. The chemical structures of the isolated compounds 1-9 were elucidated by 1D and 2D NMR and MS data analysis. All nine compounds were evaluated for their antibacterial activities against three Gram-positive and three Gram-negative bacteria. The compound 3 exhibited potent antibacterial activities against Bacillus subtilis and Micrococcus luteus. The compounds 7 and 9 displayed considerable activities against Bacillus subtilis and the others had moderate results.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2002년도 봄 학술발표회 논문집
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• pp.233-240
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• 2002

In this paper, the effects of vehicle loads on flat slab system are investigated on the basis of the previous studies for beam-girder parking structural system. The influence surfaces of flat slab for typical design section are developed for the purpose of obtaining maximum member forces under vehicle loads. In addition, the equivalent vehicle load factors for flat slab parking structures are suggested using artificial neural network. The network responses are compared with the results by numerical analyses to verify the validation of Levenberg-Marquardt algorithm adopted as training method in this paper. Many parameter studies fur the flat slab structural system show dominant vehicle load effects at the center positive moments in both column and middle strips, like the beam-girder parking structural system.

• 약학회지
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• 제37권4호
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• pp.356-361
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• 1993

A sensitive method for the determination of methamphetamine(MA) and amphetamine(AM) in hair was developed by gas chromatography/mass spectrometry using stable isotope-labeled internal standards, amphetamine-d$_{5}$ and methamphetamine-d$_{5}$. Hair sample was washed with MeOH, incubated with MeOH(I% HCI) overnight at $37^{\circ}C$ while stirring and extracted using solid phase extraction column on a vacuum manifold. The extract obtained was pentafluoropropionated, and applied to GC/MS. The calibration curves of MA and AM were linear from 2.5 to 250 ng (r>0.99 for both). The limit of detection was 0.1 ng/mg in hair and cut-off level was set at 0.25 ng/mg for both. Hair samples of 27 MA abusers showed positive results in the range 0.7 to 106.8 ng/mg. AM, its metabolite, was detected in 20 out of 27 samples. The ratio of MA versus AM was 4.6~38.3 in specimens. Hair analysis for methamphetamine by GC/MS is an effective method for identifying long-term drug abusers.

• 대한전기학회논문지
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• 제34권3호
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• pp.107-114
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• 1985

This paper presents the iterative algorithm for obtaining the ME PSE(Maximum Entropy Power Spectral Estimation) of 2-dimensional signals. This problem involves a correction matching power spectral estimate that can be represented as the reciprocal of the spectral of 2-dimensional signals. This requires two matrix inversion every iterations. Thus, we compensate the matrix to be constantly positive definite with relaxational parameters. Using Row/Column decomposition Discrete Fourier Transform, we can decrease a calculation quantity. Using Lincoln data and white noise, this paper examines ME PSE algorithms. Finally, the results output at the graphic display device. The 2-dimensional data have the 3-dimensional axis components, and, this paper develops 3-dimensional graphic output algorithms using 2-dimensional DGL(Device Independent Graphic Library) which is prepared for HP-1000 F-series computer.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 추계학술대회 논문집 전기물성,응용부문
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• pp.11-14
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• 2004

In this paper we report on development of our original mercury-less fluorescent lamps for general lighting. These new-type lamps have multi-pairs of electrodes to maintain multi-discharge paths and to keep positive column diffused state. The omission characteristics oft the lamps were investigated. These discharge lamps were operated by pulsed discharge. By using multi-pairs of electrodes for xenon pulsed discharge fluorescent lamps, higher intensity of luminance was obtained than the conventional type of lamps using single pair of electrodes. Recently this type of lamp achieved over $9000\;cd/m^2$ and 50 lm/W.