• Applied Chemistry for Engineering
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• v.35 no.4
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• pp.335-340
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• 2024

In this paper, we synthesized organic and inorganic hybrid materials to introduce antibody functionality to UIO-66 and incorporated them into a surface plasmon resonance (SPR) assay to enhance the sensitivity of detecting small molecules such as oxytocin. A biological marker peptide called oxytocin may help in the diagnosis of heart failure, Alzheimer's disease, and cancer. To detect oxytocin at concentrations as low as a few femtomole (fM), we developed a surface sandwich assay utilizing a pair of oxytocin-specific antibodies for enhancing selectivity and one of metal organic frameworks [e.g., UiO-66-(COOH)₂] possessing high porosity and surface-area as a signal amplifier. Initially, real-time SPR assays were used to confirm that each selected oxytocin-specific antibody binds strongly to oxytocin and to different binding sites on oxytocin. One of these antibodies (e.g., anti-OXT[OTI5G4]) was immobilized on the surface of a thin gold chip. Upon sequential injecting of oxytocin and the other antibody (e.g., anti-OXT[4G11]) conjugated to UiO-66-(COOH)₂ onto the surface to form the surface sandwich complex of anti-OXT[OTI5G4]/oxytocin/UiO-66-(COOH)₂-anti-OXT[4G11]), SPR changes, which varied with oxytocin concentration, were then measured in real time. The results demonstrated that sensitivity was amplified by over a million-fold compared to assays without UiO-66-(COOH)₂, enabling oxytocin detection down to approximately 10 fM.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.37 no.4
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• pp.267-274
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• 2024

Hydrogen embrittlement fracture poses a challenge in ensuring the structural integrity of materials exposed to hydrogen-rich environments. This study advances our comprehension of hydrogen-induced fracture through an integrated numerical modeling approach. In addition, it employs a ductile fracture model named the Gurson-cohesive model (GCM) and hydrogen diffusion analysis. GCM is employed as a fracture model that combines the Gurson model to illustrate the continuum damage evolution and the cohesive zone model to describe crack surface discontinuity and softening behavior. Moreover, porosity and stress triaxiality are considered as crack initiation criteria . A hydrogen diffusion analysis is also integrated with the GCM to account for hydrogen enhanced decohesion (HEDE) mechanisms and their subsequent impacts on crack initiation and propagation. This framework considers the influence of hydrogen on the softening behavior of the traction-separation relationship on the discontinuous crack surface. Parametric studies explore the sensitivity to diffusion properties and hydrogen-induced fracture properties. By combining numerical models of hydrogen diffusion and the ductile fracture model, this study provides an understanding of hydrogen-induced fracture and thereby contributes significantly to the ongoing efforts to design materials that are resilient to hydrogen embrittlement in practical engineering applications.

• Journal of Korean Society of Forest Science
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• v.66 no.1
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• pp.16-36
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• 1984

It is very important in forestry to study the shear strength of weathered granitic soil, because the soil covers 66% of our country, and because the majority of land slides have been occured in the soil. In general, the causes of land slide can be classified both the external and internal factors. The external factors are known as vegetations, geography and climate, but internal factors are known as engineering properties originated from parent rocks and weathering. Soil engineering properties are controlled by the skeleton structure, texture, consistency, cohesion, permeability, water content, mineral components, porosity and density etc. of soils. And the effects of these internal factors on sliding down summarize as resistance, shear strength, against silding of soil mass. Shear strength basically depends upon effective stress, kinds of soils, density (void ratio), water content, the structure and arrangement of soil particles, among the properties. But these elements of shear strength work not all alone, but together. The purpose of this thesis is to clarify the characteristics of shear strength and the related elements, such as water content ($w_o$), void ratio($e_o$), dry density (${\gamma}_d$) and specific gravity ($G_s$), and the interrelationship among related elements in order to decide the dominant element chiefly influencing on shear strength in natural/undisturbed state of weathered granitic soil, in addition to the characteristics of soil hardness of weathered granitic soil and root distribution of Pinus rigida Mill and Pinus rigida ${\times}$ taeda planted in erosion-controlled lands. For the characteristics of shear strength of weathered granitic soil and the related elements of shear strength, three sites were selected from Kwangju district. The outlines of sampling sites in the district were: average specific gravity, 2.63 ~ 2.79; average natural water content, 24.3 ~ 28.3%; average dry density, $1.31{\sim}1.43g/cm^3$, average void ratio, 0.93 ~ 1.001 ; cohesion, $ 0.2{\sim}0.75kg/cm^2$ ; angle of internal friction, $29^{\circ}{\sim}45^{\circ}$ ; soil texture, SL. The shear strength of the soil in different sites was measured by a direct shear apparatus (type B; shear box size, $62.5{\times}20mm$; ${\sigma}$, $1.434kg/cm^2$; speed, 1/100mm/min.). For the related element analyses, water content was moderated through a series of drainage experiments with 4 levels of drainage period, specific gravity was measured by KS F 308, analysis of particle size distribution, by KS F 2302 and soil samples were dried at $110{\pm}5^{\circ}C$ for more than 12 hours in dry oven. Soil hardness represents physical properties, such as particle size distribution, porosity, bulk density and water content of soil, and test of the hardness by soil hardness tester is the simplest approach and totally indicative method to grasp the mechanical properties of soil. It is important to understand the mechanical properties of soil as well as the chemical in order to realize the fundamental phenomena in the growth and the distribution of tree roots. The writer intended to study the correlation between the soil hardness and the distribution of tree roots of Pinus rigida Mill. planted in 1966 and Pinus rigida ${\times}$ taeda in 199 to 1960 in the denuded forest lands with and after several erosion control works. The soil texture of the sites investigated was SL originated from weathered granitic soil. The former is situated at Py$\ddot{o}$ngchangri, Ky$\ddot{o}$m-my$\ddot{o}$n, Kogs$\ddot{o}$ng-gun, Ch$\ddot{o}$llanam-do (3.63 ha; slope, $17^{\circ}{\sim}41^{\circ}$ soil depth, thin or medium; humidity, dry or optimum; height, 5.66/3.73 ~ 7.63 m; D.B.H., 9.7/8.00 ~ 12.00 cm) and the Latter at changun-long Kwangju-shi (3.50 ha; slope, $12^{\circ}{\sim}23^{\circ}$; soil depth, thin; humidity, dry; height, 10.47/7.3 ~ 12.79 m; D.B.H., 16.94/14.3 ~ 19.4 cm).The sampling areas were 24quadrats ($10m{\times}10m$) in the former area and 12 in the latter expanding from summit to foot. Each sampling trees for hardness test and investigation of root distribution were selected by purposive selection and soil profiles of these trees were made at the downward distance of 50 cm from the trees, at each quadrat. Soil layers of the profile were separated by the distance of 10 cm from the surface (layer I, II, ... ...). Soil hardness was measured with Yamanaka soil hardness tester and indicated as indicated soil hardness at the different soil layers. The distribution of tree root number per unit area in different soil depth was investigated, and the relationship between the soil hardness and the number of tree roots was discussed. The results obtained from the experiments are summarized as follows. 1. Analyses of simple relationship between shear strength and elements of shear strength, water content ($w_o$), void ratio ($e_o$), dry density (${\gamma}_d$) and specific gravity ($G_s$). 1) Negative correlation coefficients were recognized between shear strength and water content. and shear strength and void ratio. 2) Positive correlation coefficients were recognized between shear strength and dry density. 3) The correlation coefficients between shear strength and specific gravity were not significant. 2. Analyses of partial and multiple correlation coefficients between shear strength and the related elements: 1) From the analyses of the partial correlation coefficients among water content ($x_1$), void ratio ($x_2$), and dry density ($x_3$), the direct effect of the water content on shear strength was the highest, and effect on shear strength was in order of void ratio and dry density. Similar trend was recognized from the results of multiple correlation coefficient analyses. 2) Multiple linear regression equations derived from two independent variables, water content ($x_1$ and dry density ($x_2$) were found to be ineffective in estimating shear strength ($\hat{Y}$). However, the simple linear regression equations with an independent variable, water content (x) were highly efficient to estimate shear strength ($\hat{Y}$) with relatively high fitness. 3. A relationship between soil hardness and the distribution of root number: 1) The soil hardness increased proportionally to the soil depth. Negative correlation coefficients were recognized between indicated soil hardness and the number of tree roots in both plantations. 2) The majority of tree roots of Pinus rigida Mill and Pinus rigida ${\times}$ taeda planted in erosion-controlled lands distributed at 20 cm deep from the surface. 3) Simple linear regression equations were derived from indicated hardness (x) and the number of tree roots (Y) to estimate root numbers in both plantations.

• Korean Journal of Soil Science and Fertilizer
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• v.12 no.4
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• pp.169-178
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• 1980

Ammonium nitrate explosion technique was applied to seek a convenient method for the establishment of orchard on the undulating to rolling land or hill side of Pogog clay loam soil (Fine Aquic Fragiudalfs : Planosols) having high bulk density and low permeability. Explosions were made by three ammonium nitrate explosives placed in the bottom of 90cm deep auger hole with every 2m interval (Explosion I) and 4m interval (Explosion II) respectively. The effect of the explosion on physical properties of the soil was investigated and compared with the effect induced by manual digging, excavation of $1m{\times}1m$ in diameter and depth (Manual digging I) and trenching of $1m{\times}1m{\times}25m$ in width, depth, and length (Manual digging II) respectively. The results investigated after 7 months from the treatments are summarized as follows : 1. The explosion or manual digging reduced bulk density and hardness, whereas the treatments increased porosity, hydraulic conductivity, and available moisture-holding capacity of the soil. 2. The explosion of 4 m interval improved physical properties of the soil to optimum level up to 70cm of the distance from the explosion core in the range of depth 0-60cm, while in the case of depth from 60 to 100cm the optimum level was achieved only within 50cm radius. 3. When exploded in 2 m interval, the effect in the 0-60cm depth was overlapped between two explosion cores. The effect in the depth between 60 and 100cm, however, was found to be independent of the explosion intervals. 4. The manual digging was only costly and laborious but effective only within the work-up zone. 5. For the soils having bulk density higher than $1.4g/cm^3$ after the treatments, the field capacity determined 72 hours after a heavy rain was lower than the laboratory estimate at the suction of 1/3 atm. 6. The top growth of apple tree for the first year revealed that the explosion seemed better treatment than the manual digging, even though the difference was insignificant.

• Journal of Korean Society of Forest Science
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• v.86 no.2
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• pp.186-199
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• 1997

The soils derived from granite gneiss occupy almost one third of the land area in Korea. The soils under forest vegetation, formed on granite gneiss, in Sun chon-shi, Chollanam-do in southern part of Korea, were studied to evaluate the weathering and the transformation of primary minerals into secondary minerals, clay minerals. The studied soils contained large amounts of ferromagnesian minerals, weathered biotites and were well weathered, strongly acid and low in organic matters and in ration exchange capacity. The clay contents in the Bt horizon were almost two times higher than those in the C horizon. The O horizon had a thin layer which consisted of a little decomposed plant components with a granic fabric and high porosity, and showed the micromorphological characteristics of moder humus. The related distribution pattern of the E horizon were enaulic and large amounts of silts and small amounts of sand grains were another characteristics of the E horizon. The most striking micromorphological features were multilaminated clay coating and infillings in the voids in the Bt and C horizons, and generally limpid ferriargillans ejected from the biotites and imparted red color to the soils in the Bt horizon. High clay contents in the Bt horizon was not only due to clay translocation, but also due to intensive in situ mineral weathering in this horizon. The most significant pedogenic process, revealed by the petrographic microscope and SEM, was the formation of iron oxides from biotites, the formation of tubular halloysites and the weathering models of biotites; wedge weathering and layer weathering. The thick coating on the weathering biotites showed the characteristics of the weathering process and the synthetic hematites were revealed in clays by TEM. Total chemical analysis of clays revealed extensive loss of Ca, and Na and the concentration of Fe and Al. Mineralogical studies of clays by XRD showed that micas were almost completely weathered to kaolinite, vermiculite-kaolinite intergrade, hematite, gibbsite, while halloysites from other primary minerals. Some dioctahedral mica appeared to be resistant in the soils. Parent rock of the soils contained a considerable amounts of biotites and this forest soils showed especially a dominant characteristics of biotite weathering.

• Horticultural Science & Technology
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• v.33 no.3
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• pp.435-442
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• 2015

The main objective of this work was to identify and evaluate possible substrate alternatives or amendments to peat moss. This study involves the physical and chemical characterization and growth test of wood sawdust and wood fiber in order to evaluate their use as components of horticultural media. The carbohydrate content, C/N ratio, pH, phenolic compound, total porosity and water holding capacity were 58.9%, 425.1, 4.8, 181.8 ($mg{\cdot}g^{-1}$), 82.5% and 47.1% in wood sawdust and 41.1%, 240.8, 5.9, 29.8 ($mg{\cdot}g^{-1}$), 90.6% and 56.2% in wood fiber, respectively. Wood sawdust (K, $998.0mg{\cdot}100g^{-1}$ ; Ca, $1196.0mg{\cdot}100g^{-1}$; Mg, $105.6mg{\cdot}100g^{-1}$) and wood fiber (K, $1250.1mg{\cdot}100g^{-1}$; Ca, $1982.6mg{\cdot}100g^{-1}$; Mg, $173.1mg{\cdot}100g^{-1}$) showed adequate mineral elements properties compared to peat moss (K, $0.02mg{\cdot}100g^{-1}$; Ca, $0.57mg{\cdot}100g^{-1}$; Mg, $0.13mg{\cdot}100g^{-1}$) for their use as growing media. The mixtures of the horticultural media were prepared using different substrate as wood sawdust and wood fiber to grow Chinese cabbage (Brassica campestris L.) in a greenhouse. The seed germination, leaf area and stem height were 75%, $0.50cm^2$ and 2.8 cm in PS substrate (containing 30% peat moss, 10% perlite and 60% wood sawdust) and 95%, $0.65cm^2$ and 3.3 cm in PF substrate (containing 30% peat moss, 10% perlite and 60% wood fiber), respectively. The seed germination and stem height of the PF substrate (containing 30% peat moss, 10% perlite and 60% wood fiber) was higher than those in peat moss (control). Utilization of wood by-product can be considered as an alternative media component to substitute the widely using expensive peat moss.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.3
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• pp.181-188
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• 2007

Objective of this research was to identify by-product composting impacts on paddy soil properties and rice yield. Research was conducted in Iksan (soil was identified as a Jeonbug series) located in Honam plain area from 2001 to 2004. Composts, such as cow manure sawdust compost(CMSC), Chicken manure sawdust compost(ChMSC) and Pig manure sawdust compost(PMSC) were treated in the reseach plots for every, 2, and 3 year term. Some physical properties, such as, soil hardness, and bulk density tended to decrease with application of compost and decreased in order of CMSC, ChMSC, and PMSC, while surface soil depth and porosity were increased in order of CMSC, PMSC, and ChMSC. Some chemical soil properties, such as organic matter, available phosphorus, available silicate, and exchangeable cations were increased with application of compost and every year application plots. Nitrogen uptake was higher in order of CMSC, ChMSC, SF, and PMSC. Nitrogen use efficiency was higher in order of CMSC, ChMSC, SF, and PMSC. Rice yields was increased in all application plot of CMSC, in every other year application plot ChMSC and PMSC compared with SF($5.07Mg\;ha^{-1}$). Also average rice yield on years were increased in all application plot of CMSC and in every other year application plot ChMSC, while decreased in all application plot of PMSC compared with SF($5.27Mg\;ha^{-1}$). Head rice ratio and perfect grain ratio on hulled rice was high in all application plot of PMSC and in every year, in every other year app lication plot of ChMSC while its lowered percentage of 10~13 caused by application of CMSC compared with SF.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.12
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• pp.1727-1734
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• 2005

The fermentation characteristics and mono- and di-saccharides compositions during the early stage of ensiling were studied with a temperate grass, Italian ryegrass (Lolium multiflorum Lam.) and a tropical grass, guineagrass (Panicum maximum Jacq.). The laboratory silos were kept in the room set at 25$^{\circ}C$, and then were opened on 0.5, 1, 2, 3, 5 and 7 days (14 days in Italian ryegrass) after ensiling, respectively. The Italian ryegrass silage showed a fast and large pH decrease caused by a fast and large production of lactic acid during the first 5 days of ensiling and succeeded to achieve lactic acid type fermentation; high lactic acid/acetic acid and lactic acid content at the end of ensiling (14 days), low values of pH (3.74), acetic acid, ethanol and ammonia-N/total nitrogen, none or only small amounts of Butyric acid, valeric acid and propionic acid. The guineagrass silage showed a slow decrease in pH and a slow increase in lactic acid content during the full ensiling period, causing a high final pH value, low contents of lactic acid, acetic acid, total volatile fatty acids and total organic acids. In Italian ryegrass silage, mono- and di-saccharides compositions decreased largely within the initial 0.5 day (12 h) of ensiling. Sucrose disappeared rapidly within the initial 0.5 day of ensiling, but fructose and glucose contents showed an initial rise by the activity of enzymes in plant tissues, and then decreased gradually. On the other hand, the contents of monoand di-saccharides in guineagrass showed the largest decreases due mainly to plant respiration within the initial 0.5 day of ensiling, and no initial rises in fructose and glucose contents during the early stage of ensiling because of the absence of fructans which are hydrolyzed into fructose and glucose in temperate grasses. In both silages, the rate of reduction in mono- and di-saccharides compositions within the initial 5 days of ensiling was ranked in the order of glucose>fructose>sucrose, suggesting that glucose and fructose might be more favorably utilized than sucrose by microorganisms and glucose is the first fermentation substrate. It was concluded that the silage made from Italian ryegrass with high moisture content had a good fermentation quality owing to the dominance of lactic acid bacteria and active lactic acid fermentation during the initial stage of ensiling. These results can be explained by rapid plant sap liberation and the high activity of plant enzyme hydrolyzed fructans into fructose and glucose within the initial 2 days of ensiling, which stimulate the homofermentative lactic acid bacteria growth. In ensiling a temperate grass, the physical characteristics may ensure the rapid onset of fermentation phase, which results from the smaller losses of water-soluble carbohydrates during the initial stage of ensiling and providing sufficient water-soluble carbohydrates for lactic acid bacteria. The silage made from guineagrass with intermediate dry matter and high initial mono- and di-saccharides content was stable silage. This could be explained by the higher incorporation of air during the very early stage of ensiling and the restriction of cell breakdown and juice release due to the properties of a tropical grass with coarse porosity and stemmy structures. These physical characteristics delayed the onset of lactic acid bacteria fermentation phase by extending the phases of respiration and aerobic microorganisms activity, causing the higher loss of water-soluble carbohydrates and the shortage of lactic acid bacteria fermentation substrates.

• Journal of the Microelectronics and Packaging Society
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• v.24 no.4
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• pp.85-90
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• 2017

The $TiCl_4$ as a blocking material is adsorbed in the mesoporous $TiO_2$ electron transfer layer(ETL) of the Perovskite solar cell to prevent the direct contact between the FTO electrode and the photoactive layer(AL), and facilitate the movement of the electrons between $TiO_2:TiCl_4$ ETL and Perovskite AL to improve the photoelectric conversion efficiency(PCE). The structure of the perovskite solar cell is FTO/$TiO_2:TiCl_4$/Perovskite($CH_3NH_3PbI_3$)/spiro-OMeTAD/Ag. It was investigated that the dipping time of the $TiO_2$ into $TiCl_4$ aqueous solution affects on the photoelectric characteristics of the device. By the dipping for 30 minutes, the PCE of the perovskite solar cell with the $TiO_2:TiCl_4$ ETL was the highest 10.46%, which is 27% higher than the cell with $TiO_2$ ETL. From SEM, EDS, and XRD characterization on the $TiO_2:TiCl_4$ ETL and the perovskite AL, it was measured that the decrease of the porosity of the $TiO_2$ layer, the detection of the Cl component by the $TiCl_4$ adsorption, the cube-type morphology of perovskite AL, and shift of the $PbI_2$ peak of the perovskite AL. From these results, it was confirmed that the $TiO_2:TiCl_4$ ETL and the perovskite AL were formed.

• Journal of Wetlands Research
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• v.17 no.1
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• pp.1-10
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• 2015

The bleaching phenomenon of otter spraints is indicates that the age of spraints (fresh or old spraints). As previous studies on the old otter faeces (spraint) have focused only on the external changes of spraints, it has been difficult to explain the difference of the decomposition rates among old spraints. We hypothesized that the micrometeorology difference caused by various characteristics of sprainting sites such as porosity and consistency of soil could affect the extent of the decomposition rate, the solar bleaching phenomenon of spraints used as a measure of the patterns of old spraints. Sprainting sites were classified into four types (Clay, Sand, Rock and Concrete spot) and each type was divided into two groups according to the sunlight condition (Sunny site and Shady site) to measure the effect of solar radiation. Micrometeorology of the sites were evaluated by measuring temperatures and humidities of the sites during the whole season. The variations in micrometeorology of the sunny sites according to the sprainting site types were examined with correlation analyses between the factors of micrometeorology. The analyses on the bleaching phenomenon of the spraints by micrometeorology showed that the highest rate of bleaching was found at the 'Rock spot' and 'Concrete spot' of the sunny site and especially in summer, the highest rate of bleaching ($63.4{\pm}7.6%$) was found at the 'Clay spot' of the sunny sites. The lowest rate of bleaching was found at the 'Sand spot' of the sunny sites. The above findings show that the difference in bleaching rates among sprainting sites was due to the seasonal or sunny condition. In addition, we found that the differences in thermal conductivity among sprainting site characteristics have resulted in different sprainting site temperature which have affected the rate of bleaching of spraints. In conclusion, this study suggests that studies of otters on their population size and home ranges using spraints as an indirect sign, should consider the effects of sprainting site characteristics and weather conditions.