• Molecules and Cells
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• v.40 no.8
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• pp.587-597
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• 2017

MicroRNAs (miRNAs) are essential small RNA molecules that regulate the expression of target mRNAs in plants and animals. Here, we aimed to identify miRNAs and their putative targets in Hibiscus syriacus, the national flower of South Korea. We employed high-throughput sequencing of small RNAs obtained from four different tissues (i.e., leaf, root, flower, and ovary) and identified 33 conserved and 30 novel miRNA families, many of which showed differential tissuespecific expressions. In addition, we computationally predicted novel targets of miRNAs and validated some of them using 5' rapid amplification of cDNA ends analysis. One of the validated novel targets of miR477 was a terpene synthase, the primary gene involved in the formation of disease-resistant terpene metabolites such as sterols and phytoalexins. In addition, a predicted target of conserved miRNAs, miR396, is SHORT VEGETATIVE PHASE, which is involved in flower initiation and is duplicated in H. syriacus. Collectively, this study provides the first reliable draft of the H. syriacus miRNA transcriptome that should constitute a basis for understanding the biological roles of miRNAs in H. syriacus.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.324-327
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• 2009

The white flowered 'Eorayeon 1ho' is the first Kangwon National University cultivar of L. ${\times}$ formolonga, commonly known as seed propagating lily. Crosses were conducted from 1999 to 2003 among the selected selfing lines($S_3$) of L. longiflorum 'Lorina', L. ${\times}$ formolonga 'Augusta' and 'Raizan Herald'. A new cultivar was preliminarily selected as 'E-No.1' in 2003 for its stable upward flowering habit. The evaluation of characteristics was investigated from 2003 to 2004 in Chuncheon and Yungweol, Kangwon province. 'Eorayeon 1ho' blooms in early July at outside, with high resistance to Botrytis and having upward flowering habit. The plant height is 112.4 cm and the percentage of seed germination is 96.4%. This seed propagating cultivar shows good quality as a cut flower and low blasting rate.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.241-244
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• 2010

A new hybrid variety of pasqueflower, 'Yeonhong', was derived from an interspecific cross between Pulsatilla davurica and P. koreana at the Jeollanamdo Agricultural Research and Extension Services (JARES) in Naju, Korea. The original cross was performed in 2001, and the 'Yeonhong' hybrid was established in 2003 after two years of selective breeding. 'Yeonhong' is characterized by deep pink flowers and is self-incompatible. The inherent characteristics of the variety are deep yellow anthers, a deep pink stigma, six petals, and green leaf color. 'Yeonhong' blooms twice a year. The agronomic characteristics of the variety are 26.5 flowers per plant, 58.6 cm in flower height, 46.8 cm in cut flower length, 11.5 cm in bract width, and 34.4 cm in leaf length. Flower characteristics include 8.4 days of longevity for cut flowers and 34 days of longevity for bracts. The new variety is suitable for use as a cut flower.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.471-478
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• 2021

Effective microorganisms (EM), which are sources of fermentation byproducts in herbal medicine, were compared with oyster mushrooms(OM) to identify alternative larval feeds for white-spotted flower chafer (Protaetia brevitarsis seulensis). The nutritional composition of each fermented feed was analyzed. The content of crude protein, crude fat, and fiber was higher in the OM fermented feed, except for crude ash. No difference in nutritional content of larvae based on fermented feed was observed. A comparative weekly analysis of the live weights of larvae was based on the fermented feed used. The average weight was significantly higher in the feeds using EM and OM from third week of observation. The larval survival rate in the presence of fermented feed was 96.7% compared with 9.8% with non-fermented feed. The results suggest that fermented food source is essential for the growth of white-spotted flower chafer, and OM was a stable alternative to EM as a fermentation source for the survival of white-spotted flower chafer.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.2
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• pp.99-104
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• 2018

A new gerbera cultivar 'Ringgo' was bred by the 'Seohaenongsan' in 2013. 'Ma' with yellow and 'Caravan' with salmon orange, which have semi-double flower, were artificially crossed in 2010. After investigation of the characteristics for four years (from 2010 to 2013), it was selected specially for the use of cut flower. The 'Ringgo' had brilliant orange color (RHS O24A) with dark brown center. The flower was semi-double and 11.1 cm in diameter. And inner ray floret and disc diameter are 6.6 cm and 2.8 cm, respectively. It is a vigorous cultivar having a long and thick peduncle of which length and upper width were 54.7 cm and 4.6 mm, respectively. The vase life of 'Harmony' was 10.1 days, which was 1.2 days longer than the standard cultivar 'Dune'. And its average yield was 55.3 stems per plant, which were 6.6 stems more than the control.

• FLOWER RESEARCH JOURNAL
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• v.17 no.2
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• pp.75-80
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• 2009

The objective of this research was to select the native shade tolerance plant which grows well from landscape objective of the urban. Seven species native ground cover plants were evaluated on the growth and chloropyll under 0, 40, 60, 90% shading treatments. Hosta longipes was showed the quite good growth from 60% level of natural light. The plant growth of Liriope platyphylla was good within $144.2{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ intensity which is 90% shade level of natural light. But, Dryiopteris crassirhizoma was judged with shade tolerance plant which grow quite well from shading condition level of 60% degree. Carex siderosticea was showed the highest plant length and plant width in 40% shading of natural light more than at conditon too much shading. Hosta longipes, Liriope platyphylla, Dryiopteris crassirhizoma, and Carex siderosticea were as shade tolerance plant with increases chlorophyll content well to overcome a light restrictive condition and is adapted to seem with the result.

• FLOWER RESEARCH JOURNAL
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• v.19 no.1
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• pp.30-36
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• 2011

Effect of daminozide concentration and application methods, in combination with different planting period, on the growth of chrysanthemum was investigated for small pot-plant production. Daminozide, a plant growth regulator widely used for plant height control, was applied by drench or sub-application. For pot chrysanthemums of first planting time, plant height was reduced over $4,000mg{\cdot}L^{-1}$, when daminozide was drenched directly into the pot medium. However, $5,000mg{\cdot}L^{-1}$ of daminozide drench affected plant height reduction for secondarily planted pot chrysanthemum. Analysis of variance revealed the plant height was affected by planting time. For sub-application experiment of daminozide, it showed that the daminozide affected the number of flower buds formation and fresh and dry weight. These results suggest that planting period affected growth retardation of chrysanthemum. Therefore, application of growth retardant in combination with planting time and application methods may provide more efficient growth control for pot-chrysanthemum production.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.207-210
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• 2000

We investigated the structure of floral organs and possibility of seed-set to breed a variety in ginger Zingiber officinale Rosc. Floral bud was formed from collected domestic Seosan var, and foregin Thailand var, the number of florets per bud were 8 and 10 in Seosan and Thailand var, respectively, Flowering time ranged from 18 to 25 August irregularly at 4-5 pm. The flower has the long styled with fiber hairs on top of stigma and connected-two anthers. Pollens were mixed of circular and ellips shape and its extine was two layer structure. Callus formation from anther explants was effective with compact and embryogenic on N$_{6}$ medium supplemented 2 mg/l of NAA(NCM). Plant regeneration was on the MS medium with BA of 1-2 mg/l from 40 days old callus after transferred callus medium.m.

• Research in Plant Disease
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• v.21 no.4
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• pp.334-336
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• 2015

Sclerotium rot on Pulsatilla koreana was observed in the exhibition field of Jinju Agriculture Technology Center in July 2013. The infected plants showed water-soaked, blighted and rotted symptoms. White mycelial mats spread over lesions, and then numerous sclerotia were formed on flower stalk and flower stem near the soil line. The sclerotia were globoid in shape, 1-3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation on PDA was $30^{\circ}C$ and the hyphal width was $4-8{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. For molecular identification, the complete ITS rDNA sequence of the causal fungus was sequenced and analyzed. On the basis of mycological characteristics, ITS rDNA sequence analysis, and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report of sclerotium rot on Pulsatilla koreana caused by S. rolfsii in Korea.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.325-328
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• 2012

A new Dendranthema grandiflourm 'My Sun' was developed at Gyeonggi-do Agricultural Research & Extension Services (GARES), Korea in 2009. 'My Sun' was initially derived from the cross in 2005 between 'Omega Time Orange', a potted chrysanthemum cultivar with orange single type, and 'Tasman', a potted chrysanthemum cultivar with white single type in 2005. The cultivar has single type flowers with yellow petals. Trial and evaluation was conducted from 2006 to 2009 for the selection of this variety, including a shading culture in spring and a retarding culture in winter. The flowering time of 'My Sun' was October 13th, and year-round flowering is possible by shading or lighting treatment. The diameter of flower is 21.0 mm. Numbers of flowers per stem and petals per flower are 34.4 and 20.4, respectively. Its leaf color was green (Green Group 137A) and plant height was 13.3 cm. Days to flowering under the short day treatment is about 42 in spring, and numbers of branch per plant was 3.4 ea in the winter. This cultivar was resistance to white rust and consumer's preference of new pot-mum is high level than control.