• 한국작물학회지
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• 제46권6호
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• pp.439-442
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• 2001

제주지역에서 파종기이동(4월 25일, 5월 10일, 5월 25일, 6월 10일, 6월 25일)에 따른 양마의 생육특성, 수량성 및 사료 가치를 구명하기 위하여 1999년 4월 25일에서 6월 25일까지 15일 간격으로 시험하였다. 1. 초장은 4월 25일 파종구에서 278.6 cm 였던 것이 파종기가 지연됨에 따라 점차적으로 작아져서 6월 25일 파종에서 초장은 205.7 cm로 짧아졌다. 엽수, 고엽수, 경직경, 분지수 및 개체당 무게는 초장과 비슷한 경향이었다. 2. 생초, 건초, 단백질 및 TDN수량은 4월 25일 파종구에서 각각 98.5MT/㏊, 20.7MT/㏊, 2.9MT/㏊, 11.6MT/㏊로 가장 높았으나 만파할수록 점차적으로 감수되어 6월 25일 파종에서 생초수량은 45.9MT/㏊, 건초수량은 8.2MT/㏊, 단백질수량은 1.3MT/㏊, TDN수량은 5MT/㏊로 감수되었다. 엽과 경의 생초, 건초, 단백질 및 TDN수량도 만파할수록 감소되었다. 3. 파종기가 4월 25일에서 6월 25일로 지연됨에 따라 엽의 단백질 함량은 21.5%에서 24.4%로, 조지방 함량은 5.2%에서 6.1%로, NFE 함량은 39.9%에서 41.2%로, TDN 함량은 64.3%에서 69.7%로 증가한 반면에 조섬유 함량은 엽에서 20.8%에서 17.5%로, 조회분 함량은 7.9%에서 7.0%로 감소되는 경향이었다. 경의 조단백, 조지방, NFE, TDN함량은 만파 할수록 증가되었으나 조회분 및 조섬유 함량은 반대의 경향이었다. 이상의 결과로 보아 제주도 기상, 토양의 환경조건하에서 양마의 파종적기는 4월 25일로 판단되었다.

• 한국초지조사료학회지
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• 제23권3호
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• pp.143-150
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• 2003

본 시험은 제주지역의 화산회토양에서 인산시비량 차이(0, 50, 100, 150, 200, 250kg/ha)에 따른 제주재래 대두의 생육형질, 수량 및 사료가치 변화를 구명하기 위하여 2002년 5월 11일부터 2002년 9월 10일까지 시험하였다. 인산시비량이 0에서 250kg/ha로 증가함에 따라 개화기까지의 일수는 92일에서 97일로 지연되었고, 초장은 109cm에서 124cm로 커졌다. 분지수, 엽수, 경직경, 근장 및 근중 등도 인산시비량이 증가함에 따라 점차적으로 우세하였다. 생초수량은 무인산구에서 26.5MT/ha였으나 인산시비량 증가에 따라 점차적으로 증가되어 200kg과 250kg/ha 시비구에서는 각각 36.5MT/ha, 36.9MT/ha로 증수되었으나 두 시비구간의 유의한 차이는 없었다. 인산시비량이 증가함에 따라 건물수량은 6.0MT/ha에서 7.9MT/ha로, 단백질수량은 0.9MT/ha에서 1.4MT/ha로 TDN 수량은 3.4MT/ha에서 4.9MT/ha로 증수되었다. 인산시비량이 증가함에 따라 조단백질 함량은 15.5%에서 18.3%로, 조지방 함량은 2.2%에서 3.3%로, 가용무질소물은 42.5%에서 43.5%로, TDN 함량은 56.7%에서 61.9%로 증가되었으나 이와는 반대로 조회분은 32.1%에서 28.1%로, 조회분 함량은 7.7%에서 6.8%로 감소되었다. 제주지역의 화산회토양에서 제주재래 대두의 사초수량을 최고로 올릴 수 있는 적정 인산시비량은 200kg/ha로 추정할 수 있었다.

• 한국해양공학회지
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• 제33권2호
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• pp.168-177
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• 2019

KRISO (Korea Research Institute of Ship & Ocean Engineering) started a project to develop the core algorithms for autonomous intervention using an underwater robot in 2017. This paper introduces the development of the robot platform for the core algorithms, which is an ROV (Remotely Operated Vehicle) type with one 7-function manipulator. Before the detailed design of the robot platform, the 7E-MINI arm of the ECA Group was selected as the manipulator. It is an electrical type, with a weight of 51 kg in air (30 kg in water) and a full reach of 1.4 m. To design a platform with a small size and light weight to fit in a water tank, the medium-size manipulator was placed on the center of platform, and the structural analysis of the body frame was conducted by ABAQUS. The robot had an IMU (Inertial Measurement Unit), a DVL (Doppler Velocity Log), and a depth sensor for measuring the underwater position and attitude. To control the robot motion, eight thrusters were installed, four for vertical and the rest for horizontal motion. The operation system was composed of an on-board control station and operation S/W. The former included devices such as a 300 VDC power supplier, Fiber-Optic (F/O) to Ethernet communication converter, and main control PC. The latter was developed using an ROS (Robot Operation System) based on Linux. The basic performance of the manufactured robot platform was verified through a water tank test, where the robot was manually operated using a joystick, and the robot motion and attitude variation that resulted from the manipulator movement were closely observed.

• 환경생물
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• 제37권1호
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• pp.60-67
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• 2019

본 연구에서는, 한약재 부산물을 흰점박이꽃무지 유충의 먹이원으로 활용하기 위해서, 영양성분 함량, 중금속 함량, 유충의 생육효과에 미치는 영향을 분석했다. 먹이원의 영양성분을 비교한 결과, 조섬유를 제외한 조단백, 조지방, 조회분 함량이 미발효, 발효조건 한약재 부산물 모두에서 높게 나타났으며, 특히 조단백질의 함량이 매우 높게 나타났다. 먹이원의 안정성을 확인하기 위하여 한약재 부산물의 카드뮴, 납, 수은 등의 중금속 분석 결과, 모두 불검출로 먹이원으로써의 안정성을 확인할 수 있었다. 먹이원에 따른 생육 비교에서는 참나무 발효톱밥을 급이한 대조구와 한약재 부산물을 급이한 실험구는 1~3주차까지 큰 차이가 없다가 4주차 유충의 중량이 대조구는 0.137 g, 실험구는 0.671 g으로 유의한 수준에서 차이를 보이기 시작했다. 한약재 부산물 첨가별 급이에 따른 흰점박이꽃무지 유충의 중량을 비교한 결과 7주차에서 HMB40이 가장 높은 중량을 보였다. 통계분석 결과 5주차의 HMB40, HMB80의 각각의 중량과 유의적으로 차이를 보이지 않았다. 이러한 결과는 식용곤충의 출하시기를 3령 유충으로 보았을 때 5주차에 이미 출하시점에 도달했음을 의미하며, 이는 HMB40, HMB80 조건의 급이가 가장 효율적이라는 것을 나타낸다.

• Animal Bioscience
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• 제35권1호
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• pp.29-38
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• 2022

Objective: The objective of this study was to compare the feeding value, meat nutrients and associative effects of a diet comprised of various corn silages inclusion with peanut vine or wheat straw in finishing beef cattle. Methods: One hundred and eighty Simmental crossbred beef steers were blocked and assigned to the follow treatments: i) whole plant corn silage-based diet (control, WPCS), ii) mixed forages-based diet (replacing a portion of corn silage with wheat straw, WPCSW), iii) corn stalklage-based diet (CS), and iv) sweet corn stalklage-based diet (SCS). Each group consisted of 5 repeated pens with 9 steers/pen. The diets were formulated to be isonitrogenous and isoenergetic with same forage to concentrate ratio. Experimental diets were fed for 90 d. Results: The effective ruminal degradability of dry matter and crude protein were highest for WPCS diet (p<0.05), for neutral detergent fiber was highest in SCS diet (p<0.05). The average daily gain was greater for cattle offered the WPCS diet, intermediate with WPCSW and SCS and lowest with CS (p<0.001). The concentration of non-esterified fatty acid in serum was higher for steers fed with CS and SCS diets than those offered WPCS and WPCSW steers (p<0.001). The treatments did not affect the general nutritional contents and amino acids composition of Longissimus dorsi of steers (p>0.05). Conclusion: The corn silage-based diet exhibited the highest feeding value. The sweet corn stalklage and wheat straw as an alternative to corn silage offered to beef cattle had limited influence on feeding value and meat nutrients. However, the value of a corn stalklage-based diet was relatively poor. To sum up, when the high quality forage resources, such as corn silage, are in short supply, or the growth rate of beef cattle decreases in the later finishing period, the sweet stalklage and wheat straw could be used as a cheaper alternative in feedlot cattle diet without sharp reducing economic benefits.

• Animal Bioscience
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• 제35권7호
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• pp.999-1009
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• 2022

Objective: To improve the feeding value of rice straw (RS), this study evaluated the potential of rice straw fermented with Pleurotus sajor-caju (FRS) as dairy goat feed. Methods: Spent Pleurotus sajor-caju mushroom substrate was used as fungi inoculum to break the lignocellulose linkage of rice straw, which was solid-fermented at 25℃ to 30℃ for 8 weeks. The ruminal degradation of pangolagrass hay (PG), FRS, and RS were measured in situ for 96 hours in three dry Holstein cows, respectively. Effect of fungi fermented RS on milking performance was studied in feeding trials. A total of 21 Alpine goats a trial were divided into 3 groups: a control group in which PG accounted for 15% of the diet dry matter, and FRS or RS was used to replace the PG in the control group. Goats were fed twice a day under two 28-day trial in individual pens. Meanwhile, a 3×3 Latin square trial (14 days/period) was conducted to study the rumen digestion of three diets by using three fistulated dry goats. Rumen contents were collected for metabolite analyses every one to three hours on the last two days. Results: In situ study showed that fermentation could elevate the rumen degradable fraction and effective degradability of RS (p<0.05). Effective degradability of FRS dry matter was significantly increased from 29.5% of RS to 41.7%. Lactating trial results showed that dry matter intake and milk yield in the PG group and FRS group were similar and higher than those in RS group (p<0.05). The concentration of propionic acid and total volatile fatty acid in the RS group tended to be lower than those in PG group (p<0.10). There were no differences in rumen pH value and ammonia nitrogen level among the groups tested. Conclusion: Fermentation of rice straw by spent Pleurotus sajor-caju mushroom substrate could substantially enhance its feeding value to be equivalent to PG as an effective fiber source for dairy goat. The fermented rice straw is recommended to account for 15% in diet dry matter.

• Animal Bioscience
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• 제35권12호
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• pp.1871-1880
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• 2022

Objective: The primary goal was to identify the effectiveness of chemical or biological additives in delaying the deterioration of early-harvested wilted rye silage after exposure to air. Methods: Rye harvested as a whole plant at the early heading stage was wilted for 24 h. The wilted forage was divided into treatments including sodium diacetate (SDA) at 3 (SDA3) and 6 g/kg (SDA6), Lactobacillus plantarum (LP), L. buchneri (LB), or their equal mixture (LP+LB) at 1×10⁶ colony-forming unit/g fresh matter. Results: After 60 d of conservation in 20-L silos, lactic acid was greater in LP and LP+LB silages than other treatments (102 vs 90.2 g/kg dry matter [DM]). Acetic acid was greatest in SDA6 (32.0 g/kg DM) followed by LB (26.1 g/kg DM) and was lowest in LP treatment (4.73 g/kg DM). Silage pH was lower with microbial inoculation and the lowest and highest values were observed in LP and untreated silages, respectively. After 60 d, neutral detergent fiber concentration was lowest in SDA6 silages, resulting in the greatest in vitro DM digestibility (846 g/kg DM). Aerobic stability was longest in SDA6 (176 h) followed by LB treatment (134 h). Instability after aerobiosis was greatest in LP silages (68 h), about 8 h less than untreated silages. After aerobic exposure, yeast and mold numbers were lowest in SDA6 silages, resulting in DM loss minimization. Exhaustion of acetic acid and lactic acid after aerobic exposure was lowest with SDA6 but greatest with untreated and LP silages. Conclusion: Treatment of early-cut wilted rye forage with SDA at 6 g/kg resulted in silages with higher feeding value and fermentation quality, and substantially delayed deterioration after aerobic exposure, potentially qualifying SDA at this load for promotion of silage quality and delaying aerobic spoilage of early-harvested (low DM) rye forage.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권1호
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• pp.35-41
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• 2022

Mulberry is a hardy, perennial, deep-rooted plant capable of thriving under diverse agroclimatic conditions. The selection of suitable land and appropriate variety can help the sustainable mulberry field. However, no conclusive and comprehensive investigation has been conducted on the mulberry soil properties and nutritional composition of mulberry cultivars from Korea local areas in previous studies. In our study, soil properties and mulberry leaf components of Korea local mulberry farms were briefly investigated. In result, the soil organic matter (OM) content was significantly high in Buan (6.81%) and Jangseong (6.14%). In contrast, available phosphate (P₂O₅) was different in each local area. To investigate relationship between soil property and nutritive component of mulberry leaf, Cheongil leaf samples from 8 local areas were analyzed. Among the macrominerals (K⁺, Ca²⁺, Na⁺, and Mg²⁺), the concentration of K varied from (1884±9.36) mg/100 g to (2685±11.5) mg/100 g. The potassium (K⁺) of Cheongil leaf in Sangju was the highest at 2685 mg/100 g. Besides macrominerals, flavonoids, total dietary fiber contents and moisture of Cheongil leaf samples were studied in the 8 local areas. In terms of these contents, the variation was largely depending on the local areas. This study provides a possible industrial use of mulberry, and holds promise to enhance the overall profitability of sericulture.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1610-1610
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• 2001

Microbiological examination of silage is of little value in gauging the outcome of silage, and so chemical analysis is more reliable and meaningful indicator of quality. On the other hand chemical assessments of the principal fermentation products provide an unequivocal basis on which to judge quality. Livestock require energy, protein, minerals and vitamins from their food. While fresh forages provide these essential items, conserved forages on the other hand may be deficient in one or more of them. The aim of the conservation process is to preserve as many of the original nutrients as possible, particularly energy and protein components (Woolford, 1984). Silage fermentation is important to preservation of forage with respect of feeding value and animal performance. Chemical and bacteriological changes in the silo during the fermentation process can affect adversely nutrient yield and quality (Moe and Carr, 1984). Many of the important chemical components of silage must be assayed in fresh or by extraction of the fresh material, since drying either by heat or lyophilisation, volatilises components such as acids or nitrogenous components, or effects conversion to other compounds (Abrams et al., 1987). Maize silage dorms the basis of winter rations for the vast majority of dairy and beef cattle production in Uruguay. Since nutrient intake, particularly energy, from forages is influenced by both voluntary dry matter intake and digestibility; there is a need for a rapid technique for predicting these parameters in farm advisory systems. Near Infrared Reflectance Spectroscopy (NIRS) is increasingly used as a rapid, accurate method of evaluating chemical constituents in cereals and dried forages. For many years NIRS was applied to assess chemical composition in dry materials (Norris et al., 1976, Flinn et al., 1992; Murray, 1993, De Boever et al., 1996, De la Roza et al., 1998). The objectives of this study were (1) to determine the potential of NIRS to assess the chemical composition of dried maize samples and (2) to attempt calibrations on undried samples either for farm advisory systems or for animal nutrition research purposes in Uruguay. NIRS were used to assess the chemical composition of whole - plant maize silage samples (Zea mays, L). A representative population of samples (n = 350) covering a wide distribution in chemical characteristics were used. Samples were scanned at 2 nm intervals over the wavelength range 400-2500 nm in a NIRS 6500 (NIRSystems, Silver Spring, MD, USA) in reflectance mode. Cross validation was used to avoid overfitting of the equations. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The calibration statistics were R$^2$ 0. 86 (SECV: 11.4), 0.90 (SECV: 5.7), 0.90 (SECV: 16.9) for dry matter (DM), crude protein (CP), acid detergent fiber (ADF) in g kg$\^$-1/ on dry matter, respectively for maize silage samples. This work demonstrates the potential of NIRS to analyse whole - maize silage in a wide range of chemical characteristics for both advisory farm and nutritive evaluation.

• 환경생물
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• 제39권4호
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• pp.415-422
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• 2021

면화는 중요한 섬유 작물로 종자는 가축의 사료로 사용된다. 작물 생명공학은 농업 분야에서 농업적 형질과 질을 향상시키기 위해 활용되어져 왔다. 국내 식품, 사료, 가공 제품에 유전자변형(LM) 면화의 사용이 증가함에 따라 환경으로의 LM 면화의 비의도적 유출 또한 증가하고 있다. LMO 모니터링 사업에서 수집된 LM 면화를 검정하기 위하여 국내 수입 승인된 LM 면화의 검출법 개발이 필요하다. 본 연구에서는 LM 면화 MON757, MON88792, COT67B, GHB811 4종을 대상으로 동시검출법을 개발하였다. 이벤트에 대한 유전 정보는 유럽 JRC와 농림축산검역본부에서 확보하였다. LM 면화의 동시검출법 개발을 위해 이벤트 특이적인 프라이머를 설계하였으며 특이적인 증폭을 확인하였다. 특이도 검정, 무작위 표준물질 혼합물 분석, 검출한계 분석을 통하여 동시검출법의 정확도와 특이도를 검증하였다. 그 결과 본 동시검출법은 각각의 이벤트를 검출할 수 있으며 LM 표준물질을 활용하여 특이도를 검정하였다. 또한 무작위 표준물질 조합도 정확하게 검출할 수 있다. 검출한계 분석에서는 25 ng의 미량의 주형 DNA로 단회 분석으로 검출이 가능하다. 결론적으로 4종의 LM 면화 동시검출법을 개발하였으며 LM 면화 자생체 분석에 활용될 것으로 사료된다.