Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of sodium diacetate or microbial inoculants on aerobic stability of wilted rye silage

  • Li, Yan Fen (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Wang, Li Li (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Jeong, Eun Chan (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Kim, Hak Jin (Research Institute of Eco-friendly Livestock Science, Institute of GreenBio Science Technology, Seoul National University) ;
  • Ahmadi, Farhad (Research Institute of Eco-friendly Livestock Science, Institute of GreenBio Science Technology, Seoul National University) ;
  • Kim, Jong Geun (Graduate School of International Agricultural Technology, Seoul National University)
  • Received : 2022.04.13
  • Accepted : 2022.05.20
  • Published : 2022.12.01
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Abstract

Objective: The primary goal was to identify the effectiveness of chemical or biological additives in delaying the deterioration of early-harvested wilted rye silage after exposure to air. Methods: Rye harvested as a whole plant at the early heading stage was wilted for 24 h. The wilted forage was divided into treatments including sodium diacetate (SDA) at 3 (SDA3) and 6 g/kg (SDA6), Lactobacillus plantarum (LP), L. buchneri (LB), or their equal mixture (LP+LB) at 1×106 colony-forming unit/g fresh matter. Results: After 60 d of conservation in 20-L silos, lactic acid was greater in LP and LP+LB silages than other treatments (102 vs 90.2 g/kg dry matter [DM]). Acetic acid was greatest in SDA6 (32.0 g/kg DM) followed by LB (26.1 g/kg DM) and was lowest in LP treatment (4.73 g/kg DM). Silage pH was lower with microbial inoculation and the lowest and highest values were observed in LP and untreated silages, respectively. After 60 d, neutral detergent fiber concentration was lowest in SDA6 silages, resulting in the greatest in vitro DM digestibility (846 g/kg DM). Aerobic stability was longest in SDA6 (176 h) followed by LB treatment (134 h). Instability after aerobiosis was greatest in LP silages (68 h), about 8 h less than untreated silages. After aerobic exposure, yeast and mold numbers were lowest in SDA6 silages, resulting in DM loss minimization. Exhaustion of acetic acid and lactic acid after aerobic exposure was lowest with SDA6 but greatest with untreated and LP silages. Conclusion: Treatment of early-cut wilted rye forage with SDA at 6 g/kg resulted in silages with higher feeding value and fermentation quality, and substantially delayed deterioration after aerobic exposure, potentially qualifying SDA at this load for promotion of silage quality and delaying aerobic spoilage of early-harvested (low DM) rye forage.

Keywords

Acknowledgement

This study was financially supported by Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01621302).

References

  1. Bruckner P, Raymer P. Factors influencing species and cultivar choice of small grains for winter forage. J Prod Agric 1990;3: 349-55. https://doi.org/10.2134/jpa1990.0349
  2. Kim JG, Chung ES, Seo S, Ham JS, Kang WS, Kim DA. Effects of maturity at harvest and wilting days on quality of round baled rye silage. Asian-Australas J Anim Sci 2001;14:1233-7. https://doi.org/10.5713/ajas.2001.1233
  3. Auerbach H, Theobald P. Additive type affects fermentation, aerobic stability and mycotoxin formation during air exposure of early-cut rye (Secale cereale L.) silage. Agronomy 2020; 10:1432. https://doi.org/10.3390/agronomy10091432
  4. Borreani G, Tabacco E, Schmidt RJ, Holmes BJ, Muck RE. Silage review: Factors affecting dry matter and quality losses in silages. J Dairy Sci 2018;101:3952-79. https://doi.org/10.3168/jds.2017-13837
  5. McDonald P, Henderson AR, Heron SJE. The biochemistry of silage. Marlow, Bucks, UK: Chalcombe Publications; 1991.
  6. Yuan X, Wen A, Desta ST, Wang J, Shao T. Effects of sodium diacetate on the fermentation profile, chemical composition and aerobic stability of alfalfa silage. Asian-Australas J Anim Sci 2017;30:804-10. https://doi.org/10.5713/ajas.16.0773
  7. Okur AA, Gozluklu K, Okur E, Okuyucu B, Koc F, Ozduven ML. Effects of apple vinegar addition on aerobic deterioration of fermented high moisture maize using infrared thermography as an indicator. Sensors 2022;22:771. https://doi.org/10.3390/s22030771
  8. Muck RE, Nadeau EMG, McAllister TA, Contreras-Govea FE, Santos MC, Kung Jr L. Silage review: Recent advances and future uses of silage additives. J Dairy Sci 2018;101:3980-4000. https://doi.org/10.3168/jds.2017-13839
  9. Kleinschmit DH, Kung Jr L. A meta-analysis of the effects of Lactobacillus buchneri on the fermentation and aerobic stability of corn and grass and small-grain silages. J Dairy Sci 2006;89:4005-13. https://doi.org/10.3168/jds.S0022-0302(06)72444-4
  10. Danner H, Holzer M, Mayrhuber E, Braun R. Acetic acid increases stability of silage under aerobic conditions. Appl Environ Microbiol 2003;69:562-7. https://doi.org/10.1128/AEM.69.1.562-567.2003
  11. Oliveira AS, Weinberg ZG, Ogunade IM, et al. Meta-analysis of effects of inoculation with homofermentative and facultative heterofermentative lactic acid bacteria on silage fermentation, aerobic stability, and the performance of dairy cows. J Dairy Sci 2017;100:4587-603. https://doi.org/10.3168/jds.2016-11815
  12. Ferrero F, Tabacco E, Borreani G. Lentilactobacillus hilgardii inoculum, dry matter contents at harvest and length of conservation affect fermentation characteristics and aerobic stability of corn silage. Front Microbiol 2021;12:675563. https://doi.org/10.3389/fmicb.2021.675563
  13. Wilkinson JW, Davies DR. The aerobic stability of silage: key findings and recent developments. Grass Forage Sci 2013;68: 1-19. https://doi.org/10.1111/j.1365-2494.2012.00891.x
  14. Tabacco E, Righi F, Quarantelli A, Borreani G. Dry matter and nutritional losses during aerobic deterioration of corn and sorghum silages as influenced by different lactic acid bacteria inocula. J Dairy Sci 2011;94:1409-19. https://doi.org/10.3168/jds.2010-3538
  15. Driehuis F, Wilkinson JM, Jiang Y, Ogunade I, Adesogan AT. Silage review: animal and human health risks from silage. J Dairy Sci 2018;101:4093-110. https://doi.org/10.3168/jds.2017-13836
  16. Bernardes TF, Daniel JLP, Adesogan AT, et al. Silage review: Unique challenges of silages made in hot and cold regions. J Dairy Sci 2018;101:4001-19. https://doi.org/10.3168/jds.2017-13703
  17. Li YF, Jeong EC, Wang LL, Kim HJ, Ahmadi F, Kim JG. Effects of sodium diacetate or microbial inoculants on fermentative quality of wilted rye forage (Submitted).
  18. Wei SN, Jeong EC, Li YF, Kim HJ, Ahmadi F, Kim JG. Evaluation of forage production, feed value, and ensilability of proso millet (Panicum miliaceum L.). J Anim Sci Technol 2022;64:38-51. https://doi.org/10.5187/jast.2021.e131
  19. Broderick G, Kang J. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 1980;63:64-75. https://doi.org/10.3168/jds.S0022-0302(80)82888-8
  20. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  21. Yemm EW, Willis AJ. The estimation of carbohydrates in plant extracts by anthrone. Biochem J 1954;57:508-14. https://doi.org/10.1042/bj0570508
  22. Goering HK, Van Soest PJ. Forage fiber analyses (apparatus, reagents, procedures, and some applications). Washington DC, USA: US Agricultural Research Service; 1970.
  23. Woolford MK. The silage fermentation, 2 ed., New York, USA: Marcel Dekker; 1984. 350 p.
  24. Driehuis F, Oude Elferink SJWH, Van Wikselaar PG. Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteria. Grass Forage Sci 2001;56: 330-43. https://doi.org/10.1046/j.1365-2494.2001.00282.x
  25. Auerbach H, Theobald P, Kroschewski B, Weiss K. Effects of various additives on fermentation, aerobic stability and volatile organic compounds in whole-crop rye silage. Agronomy 2020;10:1873. https://doi.org/10.3390/agronomy10121873
  26. Comino L, Tabacco E, Righi F, Revello-Chion A, Quarantelli A, Borreani G. Effects of an inoculant containing a Lactobacillus buchneri that produces ferulate-esterase on fermentation products, aerobic stability, and fibre digestibility of maize silage harvested at different stages of maturity. Anim Feed Sci Technol 2014;198:94-106. https://doi.org/10.1016/j.anifeedsci.2014.10.001
  27. Jungbluth K, Maack C, Buscher W, et al. A new ex-situ method to investigate aerobic stability of maize silage faces. J Agric Sci Food Technol 2016;2:49-54.
  28. Jungbluth KH, Trimborn M, Maack G-C, et al. Effects of three different additives and two different bulk densities on maize silage characteristics, temperature profiles, CO2 and O2-dynamics in small scale silos during aerobic exposure. Appl Sci 2017;7:545. https://doi.org/10.3390/app7060545
  29. Kim JS, Lee YH, Kim YI, et al. Effect of microbial inoculant or molasses on fermentative quality and aerobic stability of sawdust-based spent mushroom substrate. Bioresour Technol 2016;216:188-95. https://doi.org/10.1016/j.biortech.2016.05.056
  30. Shan G, Buescher W, Maack C, et al. Dual sensor measurement shows that temperature outperforms pH as an early sign of aerobic deterioration in maize silage. Sci Rep 2021;11:8686. https://doi.org/10.1038/s41598-021-88082-1