• Title, Summary, Keyword: Plant extract bank

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Bioconversion of Ginsenosides in Red Ginseng Extract by Saccharomycescerevisiae and Saccharomyces carlsbergensis (홍삼농축액에서 Saccharomyces cerevisiae와 Saccharomyces carlsbergensis에 의한 Ginsenosides의 bioconversion)

  • Jang, Mi;Min, Jin-Woo;Kim, Ju-Han;Kim, Se-Young;Yang, Deok-Chun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • pp.16-16
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    • 2010
  • Ginseng(Panax ginseng C.A. Meyer) is reported to have many pharmaceutical activities. The minor ginsenosides(Rd, Rg3, Rh2 and compound K) display pharmaceutical properties superior to those of the major ginsenosides. These minor ginsenosides, which contribute a very small percentage, are produced by hydrolysis of the sugar moieties of the major ginsenosides. The pH of red ginseng extracts fermented with S. cerevisiae and S. carlsbergensis decreased rapidly during 3 days of fermentation, with no further significant change thereafter. After 20 days of fermentation, a relatively small difference remained in the acidity of extracts fermented with S. cerevisiae (0.54%) and S. carlsbergensis (0.58%). Reducing sugar in the S. cerevisiae and S. carlsbergensis extracts decreased from 25.86 to 4.54 mg/ml and 4.32 mg/ml glucose equivalents, respectively; and ethanol contents increased from 1.5% at day 0 to 16.0 and 15.0%, respectively, at 20 days. Ginsenosides Rb1, Rb2, Rc, Re, Rf, and Rg1 decreased during the fermentation with S. cerevisiae, but Rd and Rg3 increased by 12 days. Ginsenosides Rb1, Rb2, Rc, Re and Rg1 decreased gradually in the extract with S. carlsbergensis, but Rd and Rg3 were increased at 6 days and 9 days.

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Isolation and Identification of Lactobacillus kimchicus sp.nov and Bioconversion of Ginseng Saponin

  • Liang, Zhi-Qi;Kim, Ho-Bin;Kim, Yeon-Ju;Wang, Hong-Tao;Yang, Deok-Chun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • pp.15-15
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    • 2010
  • Ginseng contained many different kinds of saponin which was the most valuable for people, but its yield cannot satisfy the demand using traditional extract methods. Enzyme transformation is a conformable and highly performed method which was fit for today. A ${\beta}$-glucosidase producing bacterium ($DCY51^T$) was isolated from Korean fermented-vegetable food kimchi. The 16S rRNA gene sequence analysis revealed that the strain $DCY51^T$ belongs to the genus Lactobacillus. The highest sequence similarity was found with Lactobacillus paracollinoides LMG $22473^T$ and Lactobacillus collinoides LMG $9194^T$ with levels of 16S rDNA similarity of 97.4% and 97.3%, respectively. Based on the above results the strain $DCY51^T$ placed in the genus Lactobacillus and proposed a new species, Lactobacillus kimchicus sp. nov. $DCY51^T$ (= KCTC $12976^T$ = JCM $15530^T$). It was culture solution reacted with Red Ginseng extract and $Rb_1$, respectively. The medium of bacteria was the liquid of MRS, the temperatures of growing and reacting between bacteria liquid and saponin were samely $37^{\circ}C$, there spective reacting time were 12 hours and 48 hours. Thus we got different saponins, and TLC and HPLC analysis showed that: enzyme respectively reacted with $Rb_1$ and Red Ginseng extract got the transformed saponin, respectively. The polarity position in TLC was a little higher than Rd; and the polarity position was the same as that of Compound K's, the saponin obtained from HPLC and other experimental results was not Compound K. The constitution of its saponin was hoped to be further confirmed.

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The Screening of Antioxidant Activity in some Korean Medicinal Plants (한국 약식동원 식물자원의 항산화 활성 비교)

  • Park Hong Ju;Lee Sung Hyeon;Kim Dae Ik;Hur Eun Young;Cho Soo Muk
    • The Korean Journal of Community Living Science
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    • v.15 no.4
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    • pp.11-16
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    • 2004
  • This study was conducted to find the powerful free radical scavenging effect in methanol extracts from 35 kinds of Korean medicinal plants obtained from the Plant Extract Bank as natural plant products. Antioxidant activity of the plant extracts was determined by measuring the scavenging effect of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH). In the screening of plant extracts tested, at 200 ppm level, almost all plantex tracts showed significantly high antionidant activity. In the reinvestigation of 15 kinds of Korean medicinal plants at 50 ppm level selected as good sources with good free radical scavenging effect at 200 ppm level, the DPPH scavenging effects were similar to those of L-ascorbic acid in whole plant extract of Fragaria yezoensis (90.4%), in the plant stems and roots extract of Gingko biloba, and the leaves extract of Rhododendron mucronulatum var. ciliatum (90%). The fruits extract of Cornus officinalis showed the strongest antioxidant activity (95%). These results suggest that the methanol extracts from Korean medicinal plants can be a potential source of antioxidants that can be used to control oxidative stress in the body, But further study is needed to measure their safety for consumption

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Fruit Mediated Synthesis of Gold and Silver Nanoparticles Using Lycium chinense and Their Antimicrobial Activity

  • Chokkalingam, Mohan;Huo, Yue;Kang, Jong-Pyo;Mathiyalagan, Ramya;Kim, Yoen-Ju;Yang, Deok-Chun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • pp.94-94
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    • 2018
  • The gold (LC-AuNPs) and silver (LC-AgNPs) nanoparticles were rapidly synthesized by fruit extract of Lycium chinense within 1.15 and 25 min respectively in an eco-friendly way. The synthesized nanoparticles confirmed by relevant surface plasmon resonance peaks for gold and silver nanoparticles at 536 and 480 nm, respectively. FE-TEM results revealed that LC-AuNPs were 20-50 nm and LC-AgNPs were 50-100 nm. The maximum distribution of gold, silver elements and the crystallographic nature of synthesized were confirmed using EDX, elemental mapping and XRD. LC-AgNPs showed inhibitory activity against pathogenic microorganisms such as E. coli and S. aureus, whereas LC-AuNPs did not show inhibitory activity. The LC-AgNps nanoparticles exhibited significant cytotoxicity to human breast cancer MCF7 cell line and less cytotoxicity to non-diseased RAW264.7 (murine macrophage) cells whereas LC-AuNps showed minimal toxicity to both cell lines. In-depth research on this rapid, facile and greenery nanoparticles may play a potential role in biomedical applications.

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Rapid Screening of Apple mosaic virus in Cultivated Apples by RT-PCR

  • Ryu, Ki-Hyun;Park, Sun-Hee
    • The Plant Pathology Journal
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    • v.19 no.3
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    • pp.159-161
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    • 2003
  • The coat protein (CP) gene of Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was selected for the design of virus-specific primers for amplification and molecular detection of the virus in cultivated apple. A combined assay of reverse transcription and polymerase chain reaction (RT-PCR) was performed with a single pair of ApMV-specific primers and crude nucleic acid extracts from virus-infected apple for rapid detection of the virus. The PCR product was verified by restriction mapping analysis and by sequence determination. The lowest concentration of template viral RNA required for detection was 100 fg. This indicates that the RT-PCR for detection of the virus is a 10$^3$times more sensitive, reproducible and time-saving method than the enzyme-linked immunosorbent assay. The specificity of the primers was verified using other unrelated viral RNAs. No PCR product was observed when Cucumber mosaic virus (Cucumovirus) or a crude extract of healthy apple was used as a template in RT-PCR with the same primers. The PCR product (669 bp) of the CP gene of the virus was cloned into the plasmid vector and result-ant recombinant (pAPCP1) was selected for molecule of apple transformation to breed virus-resistant transgenic apple plants as the next step. This method can be useful for early stage screening of in vitro plantlet and genetic resources of resistant cultivar of apple plants.

Biotransformation of Liquiritin in Glycyrrhiza uralensis Fisch Extract into Liquiritigenin by Plant Crude Enzymes (식물 유래 조효소에 의한 감소 Liquiritin의 Liquiritigenin으로의 변환)

  • Park, Min-Ju;Na, In-Su;Min, Jin-Woo;Kim, Se-Yeong;Yang, Deok-Chun
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.2
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    • pp.74-78
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    • 2008
  • Liquiritin in licorice (Glycyrrhiza uralensis Fisch) extract was treated with three different plant crude enzymes (Prunus dulcis enzyme; PDE, P. armeniaca enzyme; PAE and P. persica enzyme; PPE) for biotransformation. The resulting product of liquiritin was analyzed by TLC and HPLC. The ${\beta}glucosidase$ activities of crude enzymes were 259.6 U/g (PDE), 407.6 U/g (PAE) and 445.8 U/g (PPE), respectively. The liquiritin was converted to liquiritigenin after 12 hours of incubation with the crude enzymes. Liquiritigenin content reached its maximum level after the treatment with PPE at $37^{\circ}C$.

Fruits Extracts Mediated Synthesis of Zinc Oxide Nanoparticles Using Rubus coreanus and its Catalytic Activity for Degradation of Industrial Dye

  • Rupa, Esrat Jahan;Gokulanathan, Anandapadmanaban;Ahn, Jong-Chan;Mathiyalagan, Ramya;Markus, Josua;Elizabeth, Jimenez Perez Zuly;Soshnikova, Veronika;Kim, Yeon-Ju;Yang, Deok-Chun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • pp.93-93
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    • 2018
  • This study disclosed the aqueous fruits extract of Rubus coreanus as a sustainable agent for the synthesis of Rubus coreanus zinc oxide nanoparticle (Rc-ZnO Nps) using as a reducing and capping precursor for co-precipitation method. The development of Rc-ZnO was assured by white precipitated powder and analyzed by spectroscopic and analytical instruments. The UV-visible (UV-Vis) studies indicate the maximum absorbance at 357nm which confirmed the formation of ZnO Nps and the purity, functional group and monodispersity were assured by field emission transmission electron microscopy (FE-TEM), Fourier Transform Infrared (FTIR) Spectroscopy and dynamic light scattering (DLS). The X-ray powder diffraction (XRD) data revealed the Nps is 23.16 nm in size, crystalline in nature and possess hexagonal wurtzite structure. The Rc-ZnO Nps were subjected for catalytic studies. The Malachite Green dye was degraded by Rc- ZnO NPs in both dark and light (100 W tungsten) conditions and it degraded about 90% at 4 hours observation in both cases. The biodegradable, low cost Rc-ZnO NPs can be a better weapon for waste water treatment.

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DNA Sequencing and Phylogenetic Analysis of the 18S rRNA Gene of Atractylodes japonica Koidz and Analysis of Atractylon (삽주의 18S rRNA 유전자의 염기서열 결정, 계통분류학적 분석 및 atractylon 분석)

  • Bae, Young-Min
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.1
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    • pp.26-32
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    • 2009
  • The region containing 18S rRNA gene, ITS 1 and part of the 5.8S rRNA gene of the Atractylodes japonica Koidz was amplified by PCR and the product cloned in a pBluescript SK II plasmid. DNA sequence of the cloned DNA was determined and submitted to the GenBank (accession number EU678363). Phylogenetic analysis of the ITS 1 DNA showed close similarity with the other plant species of the family Compositae. The extract of the plant materials of five different members of the family Compositae was analyzed by HPLC to detect atractylon. Extract of the A. japonica Koidz showed presence of significant amount of atractylon. However, noticeable amount of atractylon was not detected by the same analyses from the extracts of the other plants belonging to the family Compositae including Artemisia capillaris, Chrysantemum zawadskii, Eclipta prostrata or Taraxacum platycarpum.

Antioxidant activity and phenolic acid content of Gynostemma pentaphyllum leaves according to extraction conditions

  • Ko, Hyun Min;Eom, Tae Kil;Kim, Ju-Sung
    • Korean Journal of Agricultural Science
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    • v.46 no.1
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    • pp.85-92
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    • 2019
  • This study was intended to provide basic data for a health functional food study by exploring antioxidant activity of reflux extract according to the concentration of ethanol and the extract of ultrasonic waves extracted and reflux extracted under the same solvent conditions. In the same solvent condition, the reflux extract ($75.10{\pm}1.99mg$) showed a higher total phenol content than the ultrasonic wave extract ($51.74{\pm}2.28mg$). Flavonoids also had a higher reflux extract ($25.05{\pm}1.53mg$) than did ultrasonic extracts ($16.23{\pm}1.95mg$). Reflux extract according to ethanol concentration was found to have a higher phenol content than the 70% ethanol extract ($40.60{\pm}1.49mg$) in 60% ethanol extract. Flavonoid content was also similar to phenol content in reflux extract as determined by ethanol concentration from 60% ethanol ($25.05{\pm}1.53mg$) to 70% ethanol extract ($6.60{\pm}0.46mg$). In addition, the antioxidant activity (DPPH, TEAC, FRAP, ORAC) of the reflux extract in the same solvent conditions tended to be higher than that of ultrasonic extracts. Also, 60% ethanol extract had better antioxidant activity than 70% ethanol extract. However, an analysis of phenolic acid content through HPLC showed that the ultrasonic extract had a higher content in the same solvent condition than did the reflux extract. Not only the presence of phenolic acid, but also those of other compounds are believed to be attributed to the activity of antioxidants. Therefore, further studies are needed to clarify this phenomenon.