• Title/Summary/Keyword: Plant defense

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Molecular Mechanism of Plant Immune Response (식물체의 면역반응 기작)

  • Kwon Tack-Min;Nam Jae-Sung
    • Journal of Plant Biotechnology
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    • v.32 no.2
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    • pp.73-83
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    • 2005
  • Disease resistance in plants is often controlled by gene-for-gene mechanism in which avirulence (avr) gene products encoding by pathogens are specifically recognized, either directly or indirectly by plant disease resistance (R) gene products and sequential signal transduction pathways activating defense responses are rapidly triggered. As a results, not only exhibit a resistance against invading pathogens but also plants maintain the systemic acquired resistance (SAR) to various other pathogens. This molecular interaction between pathogen and plant is commonly compared to innate immune system of animal. Recent studies arising from molecular characterization of a number of R genes from various plant species that confer resistance to different pathogens and corresponding avr genes from various pathogens resulted in the accumulation of a wealth of knowledge on molecular mechanism of gene-for-gene interaction. Furthermore, new technologies of genomics and proteomics make it possible to monitor the genome-wide gene regulation and protein modification during activation of disease resistance, expanding our ability to understand the plant immune response and develop new crops resistant to biotic stress.

Infection Structures on the Infected Leaves of Potato Pre-inoculated with Bacterial Strains and DL-3-amino Butyric Acid after Challenge Inoculation with Phytophthora infestans

  • Kim, Hyo-Jeong;Jeun, Yong-Chull
    • The Plant Pathology Journal
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    • v.23 no.3
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    • pp.203-209
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    • 2007
  • Infection structures were observed using a fluorescence microscope at the penetration sites on the leaves of potato plants pre-inoculated with the bacterial strains Pseudomonas putida TRL2-3, Micrococcus luteus TRK2-2, and Flexibacteraceae bacterium MRL412, which mediated an induced systemic resistance on potato plants against late blight disease caused by Phytophthora infestans. In order to compare the infection structures on the leaves expressing systemic acquired resistance, the leaves of potato plants pre-treated with DL-3-amino butyric acid (BABA) were also observed after challenge inoculation with the same pathogen. The infection structures were investigated. The total number of germination and appressorium formation of P. infestans were counted. Furthermore, the frequencies of fluorescent epidermal cells at the penetration sites, which indicate a defense response of plant cell, were estimated. There were no differences on the germination rates of the fungal cysts among the untreated control, BABA pre-treated, and bacterial strains pre-inoculated plants. However, appressorium formation was slightly decreased on the leaves of BABA pre-treated plants compared to those of untreated as well as bacterial strains pre-inoculated plants. Furthermore, the frequencies of fluorescent cells of BABA pre-treated and bacterial strains pre-inoculated were higher than that of untreated plants, indicating an active defense reaction of the host cells against the fungal attack. On the other hand, the pre-treatment with BABA caused a stronger fluorescent of epidermal cells at the penetration sites compared to the pre-inoculation with the bacterial strains. Interestingly, the frequency of fluorescent cells by BABA, however, was lower than that by the bacterial strains. Based on the results it is suggested that the infection structures showing resistance reaction on the leaves of potato plants were different between by pre-inoculation with bacterial strains and by pre-treatment with BABA against the late blight pathogen.

The Study on Minimum Smoke Propellant to Reduce Afterburning Reaction (후연소 반응이 감소된 무연계 고체 추진제에 관한 연구)

  • Yim, Yoojin;Lee, Jongseop;Park, Euiyong;Choi, Sunghan;Yoo, Jichang;Cho, Young
    • Journal of the Korean Society of Propulsion Engineers
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    • v.17 no.5
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    • pp.10-17
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    • 2013
  • This paper describes a study on after-burning suppressant in a solid propellant to reduce the plume formed outside of rocket nozzles, which could expose the launch site and the flight track. The minimum smoke propellant to enhance the stealth ability was formulated in terms of the kinds and the effects of after-burning suppressant on the ballistic performance and the amount of primary smoke. A after-burning suppressant, $K_2SO_4$ of about 1.1% weight content was found to show profound reduction of the rocket plume, giving negligibly slight increase in pressure exponent of burning rate. Also minimum smoke propellant with less than 1.1% of $K_2SO_4$ corresponds to A-class satisfaction in primary smoke by AGARD standard.

The Road to RNA Silencing is Paved with Plant-Virus Interactions

  • Palukaitis, Peter
    • The Plant Pathology Journal
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    • v.27 no.3
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    • pp.197-206
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    • 2011
  • RNA silencing has had a large impact on biology in general, as well as on our understanding of plant-pathogen interactions, especially interactions between plants and viruses. While most of what we know about the mechanism of RNA silencing was deduced in the last 12 years, many of the interactions between plants and viruses, as well as virus-virus interactions in plants, which we now know are manifestations of RNA silencing, were the subject of decades of work from numerous laboratories. These laboratories were examining the nature and extent of phenomena such as recovery from infection, the formation of dark green islands resistant to re-infection, synergy between unrelated viruses and cross-protection between related viruses, all first described in the late 1920s. In this review, the relationships between these phenomena and their place in the defense mechanism we call RNA silencing will be described, to show how they are all linked.

Combined Genome Mapping of RFLP-AFLP-SSR in Pepper

  • Lee, Je Min;Kim, Byung-Dong
    • Genomics & Informatics
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    • v.1 no.2
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    • pp.108-112
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    • 2003
  • We have constructed a molecular linkage map of pepper (Capsicum spp.) in an interspecific $F_2$ population of 107 plants with 320 RFLP, 136 AFLP, and 46 SSR markers. The resulting linkage map consists of 15 linkage groups covering 1,720 cM with an average map distance of 3.7 cM between framework markers. Most RFLP markers ($80\%$) were pepper-derived clones and these markers were evenly distributed all over the genome. Genes for defense and biosynthesis of carotenoids and capsaicinoids were mapped on this linkage map. By using 30 primer combinations, AFLP markers were generated in the $F_2$ population. For development of SSR markers in Capsicum, microsatellites were isolated from two small-insert genomic libraries and the GenBank database. This combined map provides a starting point for high-resolution QTL analysis, gene isolation, and molecular breeding.

Isolation and Characterization of the Salicylic Acid Induced Gene in Rehmannia glutinosa by Differential Display

  • Kim, Hee-Jong;Kim, Kwon-Jong;Lee, Youn-Su
    • Mycobiology
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    • v.30 no.2
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    • pp.88-92
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    • 2002
  • Rehmannia glutinosa is a perennial medicinal plant belonging to the family Scrophulariaceae with more than 300 species known in the world, especially in temperate regions. Its roots have been used widely in Korea for medicinal purposes. However, it is commonly infected by various pathogens during storage, causing great damage to the roots, and impedes the intensive farming of the crop. Therefore, an attempt has been made to isolate and screen a resistance gene against the pathogen Fusarium oxysporum using differential display. We treated salicylic acid(SA), and isolated a resistance gene that responds to SA. As a result, we found that SA was involved in plant defense mechanism in pathogenicity tests with SA treated and non-treted plants, and we isolated a partial PR-la gene through differential display polymerase chain reaction(DD-PCR) method.

Regulation of Salicylic Acid and N-Hydroxy-Pipecolic Acid in Systemic Acquired Resistance

  • Gah-Hyun, Lim
    • The Plant Pathology Journal
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    • v.39 no.1
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    • pp.21-27
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    • 2023
  • In plants, salicylic acid (SA) is a central immune signal that is involved in both local and systemic acquired resistance (SAR). In addition to SA, several other chemical signals are also involved in SAR and these include N-hydroxy-pipecolic acid (NHP), a newly discovered plant metabolite that plays a crucial role in SAR. Recent discoveries have led to a better understanding of the biosynthesis of SA and NHP and their signaling during plant defense responses. Here, I review the recent progress in role of SA and NHP in SAR. In addition, I discuss how these signals cooperate with other SAR-inducing chemicals to regulate SAR.