• 한국균학회지
• /
• 제32권1호
• /
• pp.50-53
• /
• 2004

2003년 3월 경남 남해군 창선면 비닐하우스 및 노지재배 완두포장에서 Botrytis cinerea에 의한 완두 잿빛곰팡이병이 발생하였다. 병징은 잎, 줄기 및 꼬투리에 발생하여 병든 부분이 수침상으로 물러지고 썩으면서 병반부위에 잿빛곰팡이가 많이 생기고, 심할 경우 감염된 부위는 결국 말라죽는다. 분생포자는 무색, 단포이며 난형 또는 타원형으로 크기는 $8{\sim}17{\times}5{\sim}13\;{\mu}m$였고, 분생자경 위에 분생포자가 아주 많이 형성되었다. 분생자경은 갈색으로 격막이 있고, 폭은 $14{\sim}30\;{\mu}m$였다. 균사생육과 균핵형성 적온은 $20^{\circ}C$였다. 병반에서 분리한 병원균은 Botrytis cinerea에 의한 완두 잿빛곰팡이병으로 동정되었다.

• 생명과학회지
• /
• 제25권9호
• /
• pp.1021-1026
• /
• 2015

Invertase (β-D-fructosfuranosidase, EC 3.2.1.26)는 설탕을 포도당과 과당으로 가수분해하는 반응을 촉매한다. 3종류의 invertases [액포(수용성 산), 세포질(수용성 알칼리) 및 세포벽 결합]가 식물에서 연구되어 왔다. 우리는 순차적인 ammonium sulfate 침전, 이온교환크로마토그래피, 흡수크로마토그래피, Green-19 친화크로마토그래피 과정을 통해 완두콩(Pisum sativum L.) 발아체로부터 중성 invertase의 세포막 연결 isoform을 430배 순수 분리하였다. 분리된 세포막과 결합 된insoluble invertase (IN-INV)는 최적 pH는 중성에서 알칼리 사이(pH 6.8-7.5)로 나타났다. 이 효소는 Tris 뿐만 아니라 Hg²⁺ and Cu²⁺와 같은 중금속에 의해 저해되었다. IN-INV 의 K_m과 V_max 값은 각각 12.95 mM과 2.98 U/min으로 측정되었다. IN-INV는 기질로써 과당뿐만 아니라 라피노오스와 반응하기 때문에 진정한 β-fructofuranosidase로 판명되었다. IN-INV의 분자량 20 kDa이었다. 위 결과로 볼 때 GA 영향으로 급속히 자라는 발아체에서 단백질이 분리되었는데 특징적으로 invertase였다.

• 생명과학회지
• /
• 제21권6호
• /
• pp.901-906
• /
• 2011

완두(Pisum sativum)의 종자 경실도(硬實度, seed hardness)와 관련한 지리적 경향성을 조사하기 위하여, 한반도 남부의 북위 $34^{\circ}26'{\sim}38^{\circ}11'$ 사이에 위치한 10개 지역(춘성, 강화, 포천, 하동, 함안, 금릉, 영양. 보성, 고창, 남원)에서 수집된 지역개체군들을 대상으로 종자 내에 함유된 mannose와 galactose의 정량분석을 실시하였다. 각 지역별 mannose의 함량은 최저 5.962 mg/g (영양)에서 최고 10.351 mg/g (강화)의 범위 내에서 다양하게 나타났다. Galactose의 지역별 함량 역시, 최고 19.314 mg/g (하동)에서 최저 7.050 mg/g (영양)에 이르는 다양한 수치를 보였다. 환경적응과 관련한 생태지표로서 종자의 경실도를 반영하고 있는 mannose와 galactose의 함량비를 산출한 결과, 지역에 따라 0.66~0.94의 값을 나타내었다. Mannose와 galactose의 함량비 서열과 함께 기후형 및 온량지수 등치선 등과 같은 지리적 기후구분에 대응하여 완두의 지역개체군들은 크게 중남부형(남원, 영양, 금릉, 고창), 중부형(춘성, 강화, 포천), 그리고 남부형(함안, 하동) 등 3가지 변이유형으로 구분되었고 이질적인 1개 지역개체군(보성)은 이 같은 유형구분에서 배제하였다. Mannose와 galactose의 함량비에 관한 서열에 있어서는, 선행 연구된 야생종들과 비교하여 역의 방향성을 나타내고 있는데, 이는 재배지역의 자연환경에 더하여, 재배식물로서 지속적으로 노출되어 있는 선발 등 인위적 도태압 또한 완두의 형질분화에 중요하게 작용하고 있는 것으로 판단된다. 이상의 결과들은 완두의 지역개체군들 간에 기후형과 연관된 뚜렷한 형질 분화가 이루어졌음을 시사하고 있으며, 이는 식물의 미진화 현상과 관련한 구체적인 증거의 하나로서, 종생태학적인 의의가 큰 것으로 판단된다.

• Plant Biotechnology Reports
• /
• 제2권1호
• /
• pp.21-31
• /
• 2008

In plants, the oxygen generated by photosynthesis can be excited to form reactive oxygen species (ROS) under excessive sunlight. Excess ROS including singlet oxygen ($^1O_2$) inhibit the growth, development and photosynthesis of plants. To isolate ROS-resistant crop plants, we used paraquat (PQ), a generator of $O_2{^-}$ as a source of screening and mutagen, and obtained two PQ-resistant lines in Pisum sativum, namely R3-1 and R3-2. Both lines showed greater resistance to PQ than their wild type (WT) siblings with respect to germination, root growth, and shoot growth. Biochemical analysis showed differences in these lines, in which ROS-scavenging enzymes undergo changes with a distinguishable increase in Mn-SOD. We further observed that the cytosolic catalases (CATs) in leaves in both lines were shifted in a native-PAGE analysis compared with that of the WT, indicating that the release of bound $^1O_2$ was enhanced. Phenotypic analysis revealed distinguishable differences in leaf development, and in flowering time and position. In addition, R3-1 and R3-2 showed shorter individual inter-node lengths, dwarf plant height, and stronger branching compared with the WT. These results suggested that PQ-induced ROS-resistant Pisum have the potential pleiotropic effects on flowering time and stem branching, and that ROS including $^1O_2$ plays not only important roles in plant growth and development as a signal transducer, but also appears as a strong inhibitor for crop yield.

• 한국환경농학회지
• /
• 제4권1호
• /
• pp.43-51
• /
• 1985

식물체중의 IAA-아미노산 복합체를 확인하기 위하여 23종의 IAA-아미노산 복합체를 합성하여 이들의 UV와 IR spectra, TLC, HPLC 그리고 가수분해등 화학적 증거를 토대로, 암실재배한 완두 유묘의 줄기에서 IAA-Asp와 IAA-Glu를 잠정적으로 동정하였다. IAA-Asp는 식물체에서 IAA의 대사산물로 이미 확인된 화합물(化合物)이지만 IAA-Glu는 아직 확인되지 않고 있다.

• Journal of Plant Biology
• /
• 제39권2호
• /
• pp.123-126
• /
• 1996

The pattern of seed storage protein, vicilin, deposition and site of intracellular localization was examined in cotyledon cells of pea (Pisum sativum) seed using the immunocytochemical methods. The vicilin was confined to the cisternae fo the rough endoplasmic reticulum and dictyosome as well as protein granules newly formed in rough endoplasmic reticulum. Vacuolar protein deposites and protein bodies were also labelled by gold particles. After small protein bodies were formed in the rough endoplasmic reticulum, they were transported to large protein bodies and then fused together. Electron dense protein granule, elaborated in the dictyosome, appears to be transported from dictyosome to protein body. A few unlabelled protein granules seem to be accumulated in other type of proteins than vicilin.

• Interdisciplinary Bio Central
• /
• 제5권4호
• /
• pp.8.1-8.3
• /
• 2013

Unlike the traditional view, it is not mysterious about how G. Mendel chose the seven characters of the pea, Pisum sativum, that he studied. He first chose the pea that met three conditions he set up and repeated experiments for two years. Apparently, he knew that those characters were controlled by countable elements. Then, he derived the prediction on the basis of his idea about the elements, and selected the seven characters that satisfied the prediction. He knew "no prediction no science". In population genetics the Hardy-Weinberg principle is well known and cited in many papers and books. However, Mendel already derived the same principle in his paper, because he was acquainted also with physics and mathematics. Actually, the principle was trivial when they derived, but not at all when Mendel did. It is also well known that Mendel's laws were forgotten and rediscovered at the term of the 19th century. That may not be true either. His laws were internationally well known before the rediscovery. In fact, the 1881-year version of the Encyclopedia Britannica contains his laws.

• Journal of Plant Biology
• /
• 제32권1호
• /
• pp.33-40
• /
• 1989

Polysomal polyadenylated mRNAs which were purified from pea leaves were fractionated by sucrose grandient sedimentation. Fractions corresponding to the peak at 11.5S were found to contain mostly mRNA encoding the precursor polypeptide to the small subunit of ribulose bisphosphate carboxylase (rbcS) by in vitro translation in wheat germ extract. Double-stranded cDNA which was synthesized from the 11.5S mRNA was cloned into Hind III site of plasmid pBR 325. A cDNA clone, H24, was identified to code for rbcS. In vitro translation product of the hybridization-selected mRNA was molecular weight 20,000, presumably the precursor of rbcS. The nucleotide sequences of the H24 showed almost complete homology with the sequences encoding the transit peptide of the rbcS-3A gene which was reported by Fluhr et al.(1986).

• BMB Reports
• /
• 제28권6호
• /
• pp.523-526
• /
• 1995

Administering ${\delta}-aminolevulinic$ acid (ALA) to isolated pea (Pisum sativum L.) chloroplasts resulted in an increase of heme synthesis in the heme branch of the tetrapyrrole pathway. At 0.1 mM ALA, in the presence of 1 mM $FeSO_4$ heme synthesis was stimulated up to 7 fold of that in the absence of $FeSO_4$. N-Methylmesoporphyrin IX (NMMP), a powerful inhibitor of ferrochelatase, inhibited heme synthesis by 95% at one micromolar concentration. The addition of A TP to the chloroplasts caused not only heme synthesis, but Mg-protoporphyrin IX synthesis in the chlorophyll branch of the tetrapyrrole pathway. In the presence of NMMP, however, inhibition of Mg-protoporphyrin IX synthesis was not observed whereas heme synthesis was inhibited completely.

• Journal of Plant Biology
• /
• 제38권4호
• /
• pp.343-348
• /
• 1995

Treatment of Pisum sativum tissue with the protein kinase inhibitor staurosphorine resulted in impairment of 3H-indoleacetic acid transport in etiolated stem segments. The transport inhibitiion was accompanied by an increase in net uptake of labeled auxin in the tissue. The magnitude of auxin accumulation in tissue treated with the phytotropin N-1-naphthylphthalaic acid (NPA) which specifically blocks the efflux of auxin in the plasma membrane was reduced by the protein kinase inhibitor, suggesting that inhibition of protein phosphorylation could lead to hindrance of the auxin-exporting function of NPA receptors. The flavonoid genistein which is also known to inhibit protein kinase likewise reduced NPA-induced auxin accumulation. However, the flavonoid did not bring about auxin accumulation by itself, nor did it inhibit auxin transport. In view of the finding that the flavonoid also competes with NPA for a common binding site, a mechanism for the flavonoid effect on the NPA action will be proposed.