Identification of Amino Acid Conjugates of Indole-3-acetic Acid in Etiolated Pea(Pisum sativum L.) Shoots

완두 유묘중 IAA-amino acid 복합체의 확인

  • Park, Chang-Kyu (Department of Agricultural Chemistry, College of Agriculture, Seoul National University) ;
  • Park, Ro-Dong (Department of Agricultural Chemistry, College of Agriculture, Chonnam National University)
  • 박창규 (서울대학교 농대 농화학과) ;
  • 박노동 (전남대학교 농대 농화학과)
  • Published : 1985.06.30

Abstract

To identify amino acid conjugates of indole-3-acetic acid(IAA) in plants, 23 amino acid conjugates of IAA were synthesized and characterized by UV and IR spectroscopies, and thinlayer and high performance liquid chromatographies. In etiolated pea(Pisum sativum L. var. Sparkle) shoots, aspartic and glutamic acid conjugates of IAA were tentatively identified as metabolites of endogenous IAA by thin-layer and high performance liquid chromatography, and by alkaline hydrolysis of the conjugates.

식물체중의 IAA-아미노산 복합체를 확인하기 위하여 23종의 IAA-아미노산 복합체를 합성하여 이들의 UV와 IR spectra, TLC, HPLC 그리고 가수분해등 화학적 증거를 토대로, 암실재배한 완두 유묘의 줄기에서 IAA-Asp와 IAA-Glu를 잠정적으로 동정하였다. IAA-Asp는 식물체에서 IAA의 대사산물로 이미 확인된 화합물(化合物)이지만 IAA-Glu는 아직 확인되지 않고 있다.

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