• Mycobiology
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• v.49 no.4
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• pp.363-375
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• 2021

Penicillium species are known to be ubiquitous environmental saprophytes. In the survey of diversity of genus Penicillium, seven new records of Penicillium species belonging to section Lanata-Divaricata were isolated from freshwater and soil samples collected from different locations in Korea. Based on morphological characteristics and multilocus phylogenetic analysis of the rDNA internal transcribed spacer region (ITS), β-tubulin (BenA), and calmodulin (CaM) genes, the isolated strains were identified as P. annulatum, P. camponotum, P. echinulonalgiovense, P. globosum, P. limosum, P. onobense, and P. yunnanense, respectively. This study presents detailed phylogenetic analyses and morphological descriptions of these species that contribute to section Lanata-Divaricata in Korea.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.351-362
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• 2021

Penicillium is the most common fungal genus in various terrestrial and marine environments. The number of new and unrecorded Penicillium species from various substrates and habitats are continuously increasing. As part of a project to discover indigenous fungi in South Korea, three unrecorded Penicillium species were isolated from mudflats and seaweeds. Based on morphological characteristics and phylogenetic analyses of β-tubulin and calmodulin loci, they were identified as P. amaliae, P. infrabuccalum, and P. manginii. Here, we provide a detailed morphological description and sequence information of these previously unrecorded species.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.425-431
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• 2021

New species of arbuscular mycorrhizal fungi (Glomeromycota), Acaulospora jejuensis, was isolated from rhizosphere soils of Miscanthus sinensis in the grassland in Jeju Island of Korea. The species was identified using the morphological characteristics of the spores and the molecular phylogenetic analysis using partial DNA sequences from small subunit rDNA (SSU), internal transcribed spacer (ITS), and large subunit rDNA (LSU). Phylogenetic analysis placed the fungal species in a distinct clade within genus Acaulospora. Also, the species exhibited the morphological characteristics distinct from the other members of the genus. Therefore, Acaulospora jejuensis was described as a novel species from Korea.

• The Korean Journal of Mycology
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• v.49 no.2
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• pp.139-153
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• 2021

The Inocybaceae is a family of ectomycorrhizal fungi belonging to the Agaricales order. Most species in this family produce secondary metabolites, such as muscarine, psilocybin, and other compounds. In the present study, we conducted taxonomic studies on the Inocybaceae based on morphological and molecular phylogenetic analyses for the first time in Korea. As a result, seven species of Inocybaceae were newly recorded: Inocybe caroticolor, I. fuscidula, I. grammatoides, I. latibulosa, I. stellata, Inosperma shawarense, and Mallocybe malenconii. Among them, the genera Inosperma and Mallocybe were described for the first time in Korea. Relevant morphological characteristics, phylogenetic trees, and photographs are provided.

• Mycobiology
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• v.49 no.3
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• pp.213-222
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• 2021

Two new species of Fulvifomes are described from specimens collected in rainforests of Nonggang Nature Reserve of southern China, based on morphological characteristics and molecular phylogenetic analysis of the internal transcribed spacer (ITS) and nuclear large subunit ribosomal DNA (nLSU) sequences. Fulvifomes nonggangensis sp. nov. is characterized by perennial, sessile and solitary basidiocarps, applanate pileus, small cystidioles of 9.9-15.4 × 2.9-3.5 ㎛, large pores of 5-6 per mm, a dimitic hyphal system, and broadly ellipsoid basidiospores of 4.3-5.3 × 3.3-4.2 ㎛. F. tubogeneratus sp. nov. is characterized by perennial, sessile, and imbricate basidiocarps, a duplex context, small pores of 7-8 per mm, a dimitic hyphal system, and ovoid to subglobose basidiospores of 5.72 × 5.00 ㎛.

• Korean Journal of Breeding Science
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• v.42 no.1
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• pp.28-34
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• 2010

Diversity of 30 Korean potato cultivars was evaluated using 14 microsatellite markers. Twelve microsatellite markers representing 12 loci in the potato genome detected 84 polymorphisms among 30 cultivars and revealed alleles with a mean of 7.00 alleles per primer. The polymorphism information content (PIC) value ranged from 0.57 to 0.93 with average of 0.82. Based on polymorphism, cluster analysis was conducted by the unweighted pair-group method with arithmetic average (UPGMA) methods. Thirty potato varieties were distinctly separated into 2 groups and similarity coefficient of cluster ranged from 0.58 to 0.95. Thirty tetraploid cultivars were evaluated for six important agronomic traits. One-way analysis of variance was done to look for the degree of relationships between individual markers and traits. K1 and K2 markers showed a significant association with amylose contents, starch contents, and specific gravity.

• Mycobiology
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• v.47 no.2
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• pp.173-179
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• 2019

Cladophialophora is characterized by simple hyphomycetes with brown hyphae that give rise to branched chains of pale brown conidia and shows affinities with the Herpotrichiellaceae. A fungal strain belonging to the genus Cladophialophora was isolated from soil in Daegu, Korea. This strain produces numerous greenish to dark black lanose aerial mycelia with hair like structures. It is morphological similar to C. chaetospira, C. inabaensis, and C. multiseptata; however, the conidiophores and conidia sizes of the newly isolated strain (KNU16-032) are clearly different from them. The novelty of the strain was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region of and the partial sequence of 28S ribosomal DNA region along with the cultural characteristics. Because morphological observations and phylogenetic analysis indicated that the strain is distinct from previously known Cladophialophora species, we propose this species as a new species Cladophialophora lanosa sp. nov., and provide the detailed descriptions in this study.

• The Korean Journal of Mycology
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• v.47 no.1
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• pp.35-42
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• 2019

We isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Buan-gun, Jeollabuk-do, Korea. We identified the isolated fungal strains based on phylogenetic analysis performed using the nucleotide sequences of the internal transcribed spacer, large subunit, and beta-tubulin. We confirmed the presence of three novel endophytic fungi in Korea, namely Paracamarosporium hawaiiense, Tubakia dryina, and Zasmidium fructigenum. In this report, we described the morphological characteristics of these fungal strains and the results of their phylogenetic analysis.

• Research in Plant Disease
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• v.27 no.3
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• pp.107-114
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• 2021

During a disease survey on weeds and minor cultivated crops in Korea, a brown leaf spot disease was observed on Sonchus asper. Leaf lesions were round or irregular in shape, and grayish brown to brown with a purple margin. In severe infection, lesions enlarged and coalesced, resulting in blighting of the leaves. The isolates from these leaf lesions were identified as Alternaira sonchi based on morphological characteristics and phylogenetic analyses of Internal transcribed spacer region, Alternaria allergen a1, glyceraldehyde 3-phosphate dehydrogenase, RNA polymerase II, and translation elongation factor genes. This study provides a comprehensive description of the morphological characteristics and phylogenetical traits of A. sonchi causing brown leaf spot on S. asper in Korea.

• Mycobiology
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• v.49 no.2
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• pp.183-187
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• 2021

The Shine Muscat is a table grape, popular in South Korea for its unique mango-flavor taste. Flyspeck is a disease that is characterized by small, black, and circular specks on the grape cuticle was first observed in several commercial orchards in Sangju, South Korea, in August 2019. Here we identified the causal agent of flyspeck based on an advanced diagnosis approach, comprised of both morphological and molecular analyses. Morphological characteristics of the cultures isolated from grape flyspeck were identical to the fungus Cladosporium perangustum. The concatenated sequences of ITS, ACT, and EF1-α were used for molecular phylogenetic analysis, BLAST searches along with Bayesian inference-based phylogeny, confirmed that the causal agent of grape flyspeck is C. perangustum. The cultured fungal isolates also produced flyspeck symptoms on healthy fruits in pathogenicity tests. To the best of my knowledge, this is the first documented evidence of any Cladosporium sp. producing flyspeck symptoms on any plant.