Mycobiology

The Korean Society of Mycology (한국균학회)
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Phylogenetic and Morphological Characterization of Cladosporium perangustum Associated with Flyspeck on Shine Muscat Grapes in South Korea

  • Hassan, Oliul (Department of Ecology & Environmental System, College of Ecology & Environmental Sciences, Kyungpook National University) ;
  • Lim, Yang-Sook (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Chang, Taehyun (Department of Ecology & Environmental System, College of Ecology & Environmental Sciences, Kyungpook National University)
  • Received : 2020.08.23
  • Accepted : 2021.02.18
  • Published : 2021.04.30
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Abstract

The Shine Muscat is a table grape, popular in South Korea for its unique mango-flavor taste. Flyspeck is a disease that is characterized by small, black, and circular specks on the grape cuticle was first observed in several commercial orchards in Sangju, South Korea, in August 2019. Here we identified the causal agent of flyspeck based on an advanced diagnosis approach, comprised of both morphological and molecular analyses. Morphological characteristics of the cultures isolated from grape flyspeck were identical to the fungus Cladosporium perangustum. The concatenated sequences of ITS, ACT, and EF1-α were used for molecular phylogenetic analysis, BLAST searches along with Bayesian inference-based phylogeny, confirmed that the causal agent of grape flyspeck is C. perangustum. The cultured fungal isolates also produced flyspeck symptoms on healthy fruits in pathogenicity tests. To the best of my knowledge, this is the first documented evidence of any Cladosporium sp. producing flyspeck symptoms on any plant.

Keywords

Acknowledgement

We would like to thank all the member of Plant Pathology Lab, Kyungpook National University for their help.

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