• Title/Summary/Keyword: Phylogenetic

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Molecular Phylogeny of Poecilostome Copepods Based on the 18S rDNA Sequences

  • Kim, Jihee;Kim, Won
    • Animal cells and systems
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    • v.4 no.3
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    • pp.257-261
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    • 2000
  • To elucidate phylogenetic relationships among poecilostome families 18S rDNA sequence data were generated for seven poecilostome and one cyclopoid copopods by PCR cloning and sequencing techmiques. Phylogenetic trees were constructed by maximum parsimony, neighbor joining, and maximum likelihood methods using cyclopoid sequence as an outgroup. The results from three different analyses showed that the seven poecilostome families were eiridel into two groups: Clausidiidae-Myicolidae-Synaptiphillidae-bomolochidae and Lichomologidae-Chondracanthidae-Ergasilidae. The molecular phylogenies were consistent with those from the morphological characters. Therefore, these analyses porvide further evidence for the utility of 18S rDNA sequences in addressing phylogenetic relationships among poecilostome families.

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Phylogenetic Status of an Unrecorded Species of Curvularia, C. spicifera, Based on Current Classification System of Curvularia and Bipolaris Group Using Multi Loci

  • Jeon, Sun Jeong;Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • Mycobiology
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    • v.43 no.3
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    • pp.210-217
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    • 2015
  • A seed-borne fungus, Curvularia sp. EML-KWD01, was isolated from an indigenous wheat seed by standard blotter method. This fungus was characterized based on the morphological characteristics and molecular phylogenetic analysis. Phylogenetic status of the fungus was determined using sequences of three loci: rDNA internal transcribed spacer, large ribosomal subunit, and glyceraldehyde 3-phosphate dehydrogenase gene. Multi loci sequencing analysis revealed that this fungus was Curvularia spicifera within Curvularia group 2 of family Pleosporaceae.

Characterization of Paecilomyces variotii and Talaromyces amestolkiae in Korea Based on the Morphological Characteristics and Multigene Phylogenetic Analyses

  • Nguyen, Thi Thuong Thuong;Paul, Narayan Chandra;Lee, Hyang Burm
    • Mycobiology
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    • v.44 no.4
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    • pp.248-259
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    • 2016
  • During fungal diversity surveys of the order Eurotiales in Korea, two fungal strains, EML-DG33-1 and EML-NCP50, were isolated from samples of rat dung and fig tree leaf collected at a garden located in Gwangju in 2014. To complete the National Species List of Korea, it is a prerequisite to verify whether many questionable species, which were previously recorded but not confirmed, indeed present in Korea. Herein, the isolates were confirmed as undescribed species, Paecilomyces variotii and Talaromyces amestolkiae based on the combination of morphological and phylogenetic analyses of multigenes including the rDNA internal transcribed spacer, ${\beta}-tubulin$, and RNA polymerase II subunit 2.

Taxonomic Study on the Lichen Genus Cetrelia (Lecanorales, Ascomycota) in South Korea

  • Luo, Heng;Wei, Xin-Li;Han, Keon-Seon;Koh, Young-Jin;Hur, Jae-Seoun
    • Mycobiology
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    • v.35 no.3
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    • pp.117-123
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    • 2007
  • Seventy-two lichen specimens of Cetrelia collected in South Korea since 2003 were examined by both phenotypic and phylogenetic analyses. The phenotypic analysis was based on morphological and chemical characters, and the phylogenetic analysis was based on nrDNA ITS sequences. The result suggested that the presence and absence of isidia, soredia, lobules and medullar reaction C+ or C- are the important characters in the taxonomy of this genus. Four species of Cetrelia, C. chicitae, C. braunsiana, C. japonica, and C. pseudolivetorum have been identified in this study. Description of each species is presented with morphological and chemical characters. A key to the Cetrelia species is also presented.

Taxonomic notes on the genus Alsidium C. Agardh, including the merging of Bryothamnion Kützing (Rhodomelaceae)

  • Garcia-Soto, Gabriela;Lopez-Bautista, Juan
    • ALGAE
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    • v.33 no.3
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    • pp.215-229
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    • 2018
  • In this study, the phylogenetic relationships among the genera Alsidium C. Agardh and Bryothamnion $K{\ddot{u}}tzing$ were investigated. Phylogenetic analyses using the plastid-encoded markers rbcL, psbA, and the mitochondrial barcode region (COI-5P) resolved a well-supported clade that included the species Alsidium corallinum, Bryothamnion seaforthii, and B. triquetrum. Our results indicated that taxonomic recognition of the genus Bryothamnion is not supported and two species of Bryothamnion are reallocated to Alsidium. A reexamination of the morphological definition of Alsidium is provided with an updated diagnosis of the genus and a morphology-based comparison of species that are currently circumscribed under this generic name. Furthermore, we reviewed morphological differences and similarities between Alsidium and the genus Digenea, both belonging to the tribe Alsidieae, discussing the most relevant morphological characters.

A Newly Recorded Sea Star of the Genus Luidia (Asteroidea: Paxillosida: Luidiidae) from the Korea Strait, Korea

  • Kim, Donghwan;Kim, Minkyung;Shin, Sook
    • Animal Systematics, Evolution and Diversity
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    • v.33 no.2
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    • pp.131-135
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    • 2017
  • Asteroid specimens of the genus Luidia were collected at a depth of 95-100 m in the Korea Strait by bottom trawling in April 2016. The specimens were identified as Luidia avicularia Fisher, 1913 (Luidiidae: Paxillosida) based on morphological characteristics and molecular phylogenetic analyses, and the species is new to the Korean fauna. A 648-bp partial nucleotide sequence of mitochondrial cytochrome c oxidase I (mt-COI) gene was obtained from Korea, and then was compared to sequences of related species stored in GenBank using molecular phylogenetic analyses. No sequence differences were detected between the L. avicularia mt-COI gene sequences from Korea and China, and the species described in this report was clearly distinct from L. maculata, which was previously reported in Korean fauna. Three Luidia species have been reported in Korea.

Full-length ORF2 sequence-based genetic and phylogenetic characterization of Korean feline caliciviruses

  • Kim, Sung Jae;Kim, Cheongung;Chung, Hee Chun;Park, Yong Ho;Park, Kun Taek
    • Journal of Veterinary Science
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    • v.22 no.3
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    • pp.32.1-32.8
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    • 2021
  • Feline calicivirus (FCV) is a highly infectious pathogen in cats and widely distributed worldwide with high genetic variation. Full-length open reading frame 2 of 5 from recently isolated Korean FCV isolates were sequenced and compared with those of global isolates. The results of phylogenetic analysis supported dividing global FCV isolates into two genogroups (type I and II) and demonstrated the presence of genogroup II in Korea, indicating their geographic spread in East Asia. High sequence variations in region E of the FCV isolates emphasizes that a novel vaccine needs to be developed to induce protective immunity against various FCV strains.

The complete chloroplast genome of Limonium tetragonum (Plumbaginaceae) isolated in Korea

  • KIM, Yongsung;XI, Hong;PARK, Jongsun
    • Korean Journal of Plant Taxonomy
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    • v.51 no.3
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    • pp.337-344
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    • 2021
  • The chloroplast genome of Limonium tetragonum (Thunb.) Bullock, a halophytic species, was sequenced to understand genetic differences based on its geographical distribution. The cp genome of L. tetragonum was 154,689 bp long (GC ratio is 37.0%) and has four subregions: 84,572 bp of large single-copy (35.3%) and 12,813 bp of small single-copy (31.5%) regions were separated by 28,562 bp of inverted repeat (40.9%) regions. It contained 128 genes (83 protein-coding genes, eight rRNAs, and 37 tRNAs). Thirty-five single-nucleotide polymorphisms and 33 INDEL regions (88 bp in length) were identified. Maximum-likelihood and Bayesian inference phylogenetic trees showed that L. tetragonum formed a sister group with L. aureum, which is incongruent with certain previous studies, including a phylogenetic analysis.

Phylogeny and Morphology of Sarcopodium terrigenum sp. nov., a Novel Fungal Species Isolated from Soil in Korea

  • Elderiny, Nabil Salah;Das, Kallol;Lee, Seung-Yeol;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.49 no.2
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    • pp.175-181
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    • 2021
  • A fungal strain was isolated from a soil sample collected in Korea and designated as YW23-8. Based on a sequence analysis of the internal transcribed spacer (ITS) regions, the isolate was assigned to the genus Sarcopodium. Moreover, a phylogenetic analysis based on the concatenated nucleotide sequences of the ITS regions and the large subunit of the nuclear ribosomal RNA (LSU) gene showed that the strain YW23-8 occupies a distinct phylogenetic position within Sarcopodium. The isolate had significant differences from its closest neighbors, S. circinosetiferum, S. circinatum, S. macalpinei, and S. vanillae. Morphological features such as different conidial structures, the absence of septation in conidia, and the presence of milky white watery droplets along with the results of the phylogenetic analysis clearly distinguish YW23-8 from the closest Sarcopodium species. We therefore conclude that strain YW23-8 represents a novel species of the genus Sarcopodium for which we propose the name Sarcopodium terrigenum.

Morphological and Phylogenetic Characteristics of Tuber himalayense Collected from Rhizosphere of Quercus dentata in Korea

  • Park, Hyeok;Gwon, Ju-Hui;Lee, Jong-Chul;Kim, Hyun Suk;Seo, Geon-Sik;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.49 no.1
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    • pp.101-108
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    • 2021
  • We collected the ascomata of Tuber species from the rhizosphere of Quercus dentata in Danyang, Korea. We observed the morphological characteristics of ectomycorrhizal roots and ascomata, and identified the species based on the results of the phylogenetic analysis conducted using the DNA sequences of an internal transcribed spacer, a large-subunit rDNA, translation elongation factor 1-α DNA (TEF1), and MAT. Finally, we identified the fungal species as Tuber himalayense B.C. Zhang & Minter, which has not been recorded previously in Korea. We evaluated the morphological characteristics and conducted phylogenetic analysis of the ascoma and mycorrhiza (associated with Q. dentata) of T. himalayense.