• Proceedings of the Korean Society of Life Science Conference
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• 2008.10a
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• pp.64.1-64.1
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• 2008

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.3
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• pp.220-226
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• 2005

We investigated the effect of $O_3$ on the photosynthetic characteristics of oriental plane (Platanus orientalis L.) that is used as a side tree or ornamental tree in Korea. Two-year-old oriental plane seedlings were transplanted to pots and transferred into a closed $O_3$ chamber, Photosynthetic pigment content and photosynthetic characteristics of leaves were measured every three weeks during 100 ppb $O_3$ fumigation. There was no visible foliar injury by $O_3$ exposure and the content of photosynthetic pigments did not show significant differences between control and $O_3$-treated seedlings. But photosynthetic rate, stomatal conductance, and water use efficiency in leaves of $O_3$-treated seedlings were reduced after six weeks of ozone fumigation. In addition, reduction of carboxylation efficiency and photochemical efficiency was observed in leaves of $O_3$-treated seedlings after three weeks and six weeks. In accordance with our results, carboxylation efficiency, the most sensitive parameter to $O_3$ stress, was considered to be a suitable indicator of $O_3$ sensitivity.

• ALGAE
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• v.24 no.2
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• pp.93-104
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• 2009

Imaging-PAM fluorometry was used to assess the chlorophyll a fluorescence parameter ${\Phi}_{PSII}$ (effective quantum yield) in Frcus vesiculosus. F. disttchus. ssp. distichus and AscophyIIum nodosum. The objective was to show variadon in fluorescence yield associated with age and frond organ, and to illustrate the spatial scales at which photosynthetic parameters vary on fucoid thalli. In addition, our species represented taxa in different but related genera, species with different ecoloeies (rock pool and non rock pool species), morphologies (with and without air bladders) and longevities (several to 20 or more years). A further objective was to determine the extent to which photosynthetic parameters reflected these differences- Effective quantum yield declined substantially with age in F. vesiculosus and F. distichus ssp. distichus, whereas ${\Phi}_{PSII}$ in A. nodosum was maximal after three years. In A. nodosum ${\Phi}_{PSII}$ was still high in branch segments at least seven years old. Older branches of A. nodosum showed relatively higher and more homogeneous photosynthetic capacity relative to Fucus species. Surfaces of air bladders in A. nodosum and F. vesicu- losus had ${\Phi}_{PSII}$ that was not significantly different from the highest rates, achieved in these species. The heterogene- ity of photosynthetic efficiency is consistent with morphological and developmental differences among the species and their ecology. in particular the longevity of A. nodosum fronds.

• Journal of Microbiology and Biotechnology
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• v.12 no.6
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• pp.962-971
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• 2002

The current study proposes a simple monodimensional model to estimate the linear growth rate of photosynthetic microorganisms in flat-plate photobioreactors (FPPBRs) during batch cultivation. As a model microorganism, Chlorella kessleri was cultivated photoautotrophically in FPPBRs using light-emitting diodes (LEDs) as the light sources to provide unidirectional irradiation in the photobioreactors. Various conditions were simulated by adjusting both the intensity of the light and the height of the culture. The validity of the proposed model was examined by comparing the linear growth rates measured with the predicted ones obtained from the proposed model. Accordingly, the value of $\frac{K\cdot\mu m}{\alpha\cdot L}log(I_0\cdot{I_s}^{\varepsilon 1)\cdot {I_c}^{-\varepsilon})$ was proposed as an approximate index for strategies to obtain the maximal lightn yield under light-limiting conditions for high-density algal cultures and as a control parameter to improve the photosynthetic productivity and efficiency.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.208-214
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• 2022

Drought stress is a condition that occurs frequently in the field, it reduces of the agricultural yield of field crops. The aim of the study was to screen drought-adapted genotype of Italian rye grass. The experiments were conducted between the two Italian ryegrass (Lolium multiflorum L.) cultivars viz. Hwasan (H) and Kowinearly (KE). The plants were exposed to drought for 14 days. The results suggest that the morphological traits and biomass yield of KE significantly affected by drought stress-induced oxidative stress as the hydrogen peroxide (H₂O₂) level was induced, while these parameters were unchanged or less affected in H. Furthermore, the cultivar H showed better adaptation by maintaining several physiological parameter including photosystem-II (Fv/Fm), water use efficiency (WUE) and relative water content (RWC%) level in response to drought stress. These results indicate that the cultivar H shows improved drought tolerance by generic variation, improving photosynthetic efficiency and reducing oxidative stress damages under drought stress. These findings can be useful to the breeder and farmer for improving drought tolerance in Italian rye grass through breeding programs.

• Journal of Marine Bioscience and Biotechnology
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• v.14 no.2
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• pp.112-123
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• 2022

Photosynthetic bacteria (PSB) play an important role in water purification, and their application is beneficial for sustainable aquaculture. However, maintaining the microbial stability of PSB from subculturing to preservation is a challenging task. Since improvement in the microbial stability of PSB is a crucial parameter, optimized conditions for cell immobilization and lyophilization were investigated. In PSB immobilization, 0.1-M CaCl₂ was found to be the most effective divalent metal ion solution in terms of cost-effectiveness, resulting in beads with a 4-mm diameter and high loading (1.91×10⁹ CFU/mL) of viable cells. Maintenance of cell viability, external appearance, and color of PSB beads was best in 3.5% NaCl during storage. In lyophilization, the addition of skim milk (9%) and dextrose (2%) as cryoprotective additives allowed the highest cell viability. Over an 18-week shrimp breeding period, when optimally manufactured beads and lyophilized powder of PSB were applied to shrimp aquaculture water, NH⁴⁺, NO₃^-, and NO₂^- were more effectively removed by 55%, 100%, and 100%, respectively, compared to controls. Thus, microbial stability of PSB through optimized cell immobilization and lyophilization was successfully enhanced, enabling a wide application.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.303-309
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• 1985

Continuous production of hydrogen by Ca alginate-immobilized photosynthetic bacteria was studied in a packed-bed bioreactor. The dilution rate and input concentration of carbonaces substrate were selected as operating parameters. To choose the strain for immobilization, hydrogen productivities of Rhodopseudomonas caposulata 10006 and Rhodospirillum rubrum KS-301 were compared through preliminary batch cultures of their free cells: the former was found to show better hydrogen productivity in spite of its lower specific growth rate. For the continuous production of hydrogen by immobilized R capsulata, the optimum dilution rate was about 0.84 h$^{-1}$ . The Immobilized tells gave better hydrogen yield and conversion efficiency than free ones. And a kinetic parameter K'$_{m}$ was determined for the packed-bed bioreactor, being practically constant for a specific range of dilution rates.s.

• Korean Journal of Agricultural and Forest Meteorology
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• v.12 no.3
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• pp.173-182
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• 2010

This study was conducted to investigate ozone sensitivity of physiological indicators and the difference in ozone tolerance of 8 families of Sophora japonica seedlings on the basis of the standardized physiological indicators. After ozone treatment, photosynthetic parameters, photosynthetic pigments and malondialdehyde (MDA) content, and antioxidative enzyme activities were analyzed from the leaves of S. japonica seedlings. Ozone tolerance indices among 8 families were calculated with the standardized physiological parameters. In addition, the reduction of carboxylation efficiency and apparent quantum yield were observed in the leaves of seven families, except for family No. 6 and 7, respectively. The apparent quantum yield varied from -27% to -61% of the control seedlings. Photosynthetic pigment content differed significantly among 8 families, but was not affected significantly by ozone treatment. Superoxide dismutase (SOD) activity increased from 7% to 64% after ozone exposure, and significant difference existed among 8 families. Ascorbate-peroxidase (APX) activity of 8 families increased by ozone treatment, and the activity of family No. 7 showed the highest increase (218%) in comparison to their respective control plants. On the basis of the standardized indices, family No. 6 showed the lowest tolerance by indicating higher reduction of both photosynthetic parameters and pigment content and lower increase of antioxidative enzyme activities. On the contrary, family No. 7 showed the highest tolerance as indicated by lower reduction of photosynthetic parameters, higher amounts of photosynthetic pigments, and higher enzyme activity.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.3
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• pp.189-193
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• 2001

Critical cell density (CCD), the maximum cell concentration without mutual shading in algal cultures, can be used as a new operating parameter for high-density algal cultures and for the application of the flashing light effect on illuminated algal cultures. CCD is a function of average cell volume and light illumination area. The CCD is thus proposed as an index of estimation of mutual shading in algal cultures. Where cell densities are below the CCD, all the cells in photobioreactors can undergo photosysnthesis at their maximum rate. At cell densities over CCD, mutual shading will occur and some cells in the illumination chamber cannot grow photoautotrophically. When the cell concentration is higher than the CCD, specific oxygen production rates under flashing light were higher than those under continuous light. The CCD was found to be a useful engineering parameter for the application of flashing light, particularly in high-density algal cultures.

• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.878-884
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• 2010

Ozone sensitivity of physiological indicators and the difference of ozone tolerance on 4 families of Quercus aliena seedlings were investigated on the basis of the standardized physiological indicators. Photosynthetic parameters, photosynthetic pigment and malondialdehyde (MDA) content, and antioxidative enzyme activities were measured or analyzed from the leaves of Q. aliena seedlings at the end of ozone fumigation, and ozone tolerance indices among 4 families were calculated with the standardized physiological parameters. After ozone treatment, the reduction of carboxylation efficiency was observed in the leaves of four families, and their reduction were ranged from -24.1% to -56.9% of control seedlings. Photosynthetic pigment content differed significantly among 4 families and treatments. The reduction of total chlorophyll content showed the highest in family SU4 (-40.6%) and the lowest family US2 (-18.8%). Ascorbate-peroxidase (APX) activity showed significant difference among families and treatments, and increased as compared with control in three families, except for family US2. On the basis of the physiological indices, ozone tolerance of four families was ranked in the order of US1 > SU4 > US2 > SU1. In conclusion, photosynthetic parameters, pigment content and APX activity were recommended as appropriate indicators to assess the tolerance against ozone stress of Q. aliena.