Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Ozone Sensitivity of Physiological Indicators for Stress Evaluation in Four Families of Quercus aliena Blume

갈참나무 4가계에서 스트레스 평가용 생리 지표들의 오존 민감성

  • Kim, Du-Hyun (Department of Forest Resources Development, Korea Forest Research Institute) ;
  • Han, Sim-Hee (Department of Forest Resources Development, Korea Forest Research Institute) ;
  • Lee, Jae-Cheon (Department of Forest Resources Development, Korea Forest Research Institute)
  • 김두현 (국립산림과학원 산림자원육성부) ;
  • 한심희 (국립산림과학원 산림자원육성부) ;
  • 이재천 (국립산림과학원 산림자원육성부)
  • Received : 2010.08.20
  • Accepted : 2010.11.11
  • Published : 2010.12.30
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Abstract

Ozone sensitivity of physiological indicators and the difference of ozone tolerance on 4 families of Quercus aliena seedlings were investigated on the basis of the standardized physiological indicators. Photosynthetic parameters, photosynthetic pigment and malondialdehyde (MDA) content, and antioxidative enzyme activities were measured or analyzed from the leaves of Q. aliena seedlings at the end of ozone fumigation, and ozone tolerance indices among 4 families were calculated with the standardized physiological parameters. After ozone treatment, the reduction of carboxylation efficiency was observed in the leaves of four families, and their reduction were ranged from -24.1% to -56.9% of control seedlings. Photosynthetic pigment content differed significantly among 4 families and treatments. The reduction of total chlorophyll content showed the highest in family SU4 (-40.6%) and the lowest family US2 (-18.8%). Ascorbate-peroxidase (APX) activity showed significant difference among families and treatments, and increased as compared with control in three families, except for family US2. On the basis of the physiological indices, ozone tolerance of four families was ranked in the order of US1 > SU4 > US2 > SU1. In conclusion, photosynthetic parameters, pigment content and APX activity were recommended as appropriate indicators to assess the tolerance against ozone stress of Q. aliena.

오존에 노출된 갈참나무 4가계의 오존 내성 차이와 생리적 지표들의 오존 민감성은 광합성 특성, 광색소 함량, MDA 함량 및 항산화효소 활성과 같은 생리적 지표들을 표준화하여 결정하였다. 오존 처리 후, 갈참나무의 잎내 탄소고정효율 감소는 4가계 모두에서 관찰되었으며, 그들의 감소율은 대조구의 -24.1%에서 -56.9% 범위를 보였다. 광색소 함량은 가계 간 및 처리 간 차이가 뚜렷하였으며, 총 엽록소 함량의 감소는 SU4 가계에서 가장 높았고 (-40.6%), US2 가계에서 가장 낮았다(-18.8%). APX 활성은 가계 간 및 처리 간 차이를 뚜렷하게 보였으며, US2 가계를 제외한 3가계에서 대조구보다 높게 나타났다. 생리적 지표들을 기준으로 한, 갈참나무 4가계의 오존 내성은 US1 > SU4 > US2 > SU1의 순으로 나타났다. 결론적으로 광합성 특성, 광색소 함량과 APX 활성은 갈참나무의 오존 스트레스 내성을 평가하는데 적절한 지표로 판단된다.

Keywords

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