Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Antioxidant Effect of the Fraction Isolated from Pyrus ussuriensis Leaves

산돌배나무(Pyrus ussuriensis) 잎 분획물의 항산화 효과

  • 이창언 (대구한의대학교 화장품약리학과) ;
  • 김영훈 ((주)메디웨이코리아 피부과학연구소) ;
  • 이병근 (영남대학교 산림자원학과) ;
  • 이도형 (영남대학교 산림자원학과)
  • Received : 2010.08.26
  • Accepted : 2010.10.05
  • Published : 2010.12.30
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Abstract

This study was conducted to confirm the application as ingredients of cosmetics through an examination of the function for anti-oxidant activity of the fraction isolated from Pyrus ussuriensis leaves. The dried leaf of Pyrus ussuriensis were extracted with acetone-$H_2O$ (6:4, v/v), concentrated and fractionated with the upper layer of acetone on a separatory funnel. Each fraction was freeze dried, then a portion of acetone soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents and also used the MIC-gel using a series of aqueous methanol as developing solvent. The isolated compounds were identified by silica-gel TLC. The concentration of total phenolic compound of Pyrus ussuriensis acetate soluble fraction was high, 914 mg/g. The results obtained from the analyses of the anti-oxidanat effects of Pyrus ussuriensis acetate fraction can be summarized as follows: In the result of DPPH scavenging radical activity, Pyrus ussuriensis acetate soluble fraction showed more than 80% at 100 ppm. SOD-like activity of one of Pyrus ussuriensis acetate soluble fractions was 77% at 1000 ppm. Xanthine oxidase inhibition of Pyrus ussuriensis acetate soluble fraction was 38% at 100 ppm. From these results, we confirmed that acetate fraction of Pyrus ussuriensis has a great potential as a natural ingredients with a natural antioxidant and antimicrobial source.

본 연구는 산돌배나무(Pyrus ussuriensis) 잎에서 추출한 분획물의 항산화능을 조사하여 화장품소재로 적용 가능성을 확인하기 위하여 수행하였다. 산돌배나무 잎을 채취하여 건조시킨 후 분쇄하여 60% acetone-$H_2O$(6:4, v/v)로 추출하고, 아세톤 가용부를 분획하여 동결건조 시켰다. 아세톤 및 물 가용부를 Sephadex LH-20으로 충진한 칼럼에서 메탄올 MeOH을 용출용매로 사용하여 칼럼크로마토그래피를 실시하고 또한 MeOH를 전개용매로 사용한 MICgel을 이용했다. 단리된 화합물들은 Silica-gel TLC로 확인하였다. 폴리페놀 함량은 산돌배나무 잎의 아세톤 분획물에서 914 mg/g으로 정량되어 높은 함량이었다. 산돌배나무 잎의 아세톤 분획물을 이용하여 항산화효과를 분석하여 얻은 결론을 요약하면 다음과 같다. 전자공여능의 경우 산돌배나무 잎의 아세톤 분획물의 100 ppm농도에서 80%이상의 전자공여능을 나타내었다. 산돌배나무 잎의 아세톤 분획물 중 한 분획물의 Superoxide dismutase(SOD) 유사활성은 1000 ppm에서 77%의 활성을 보였다. 분획물의 Xanthine oxidase 저해능은 10 ppm에서 38%의 저해률을 보였다. 이상의 결과로 미루어 보아 산돌배나무 잎의 아세톤 분획물은 항산화효과를 가진 천연소재임을 확인할 수 있었다.

Keywords

References

  1. 김태정. 1996. 한국의 자원식물 II. 서울대학교출판부. 삼성문화인쇄. 186p.
  2. Aoshima, H., Tsunoue, H., Koda H. and Y. Kiso. 2004. Aging of whiskey increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Journal of Agricultural Food Chemistry 52: 5240-5244. https://doi.org/10.1021/jf049817s
  3. Association of Analytical Chemists. 1984. Official methods of analysis of the AOAC. 14th ed. Washington (DC): Association of Analytical Chemists INC.
  4. Bauer, G. 2000. Reactive oxygen and nitrogen species: Efficient, selective and interactive signals during intercellular induction of apoptosis. Anticancer Research 20: 4115-4139.
  5. Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  6. Brand-Wiliams, W., Cuvelier, M.E. and Berste, C. 1995. Use free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft und Technologie 28: 25-30.
  7. Choi, H.J., Park, J.H., Han, H.S., Son, J.H., Son, G.M., Bae, J.H. and Choi, C. 2004. Effects of polyphenol compound from Korean pear on lipid metabolism. Korean Journal of Food Science and Technology 33: 299-304.
  8. Choinacki, T. and Vogtman, T. 1984. The occurrence and seasonal distribution of $C_{50}-C_{60}$ polyphenols and of $C_{100}$ and similar long-chain polyphenols in leaves of plant. Acta Biomaterialiia. Acta Biochim. Polinica 31: 115-126.
  9. Frankel, E.N. 1996. Antioxidants in lipid foods and their on food quality. Food Chemistry 57: 51-54. https://doi.org/10.1016/0308-8146(96)00067-2
  10. Hatano, T., Yasuhara, T., Fukuda, T., Noro, T. and Okuda, T. 1989. Phenolic constituents of licorice. II. Structures of licopyranocoumarin, licoarylcoumarin and glisoflavone, and inhibitory effects of licorice phenolics on xanthine oxidase. Chemical & Pharmaceutical Bulletin 37(11): 3005-3009. https://doi.org/10.1248/cpb.37.3005
  11. Hwang, H.S., Lee, Y.K. and Lee, K.G. 2006. Fractionation of Banaba Leaves Extract (Lagerstroemia speciosa L. Pers) and its Antioxidant Activity. Food Engineering Progress 10: 120-124.
  12. Ito, N., Fukushima, S., Hasegawa, A., Shibata, M. and Ogis, O.T. 1983. Carcinogenicity of butylated hydroxy antisole in F344 rats. Health and National Cancer Institute 70: 343-347.
  13. Jeong, C.H., Kang, S.T., Joo, O.S., Lee, S.C., Shin, Y.H., Shin, K.H., Cho, S.H., Choi, S.G. and Heo, H.J. 2009. Phenolic content, antioxidant effect and acetylcholinesterase inhibitory activity of Korean commerG. ltyree, PtivitOolong, and Blac teas. Korean Journal of Food Preservation 16(2): 230-237.
  14. Kelley, W.N and Wyngaarden, J.B. 1974. Enzymology of gout. Advances in Enzymology and Related Areas of Molecular Biology 41: 1-33.
  15. Kim, S.R, Ha, T.Y., Song, H.N., Kim, Y.S and Park, Y.K. 2005. Comparison of nutritional composition and antioxidative activity for kabocha squash and pumpkin. Korean Journal of Food Science and Technology 37: 171-177.
  16. Lee, J.H and Lee, S.R. 1994. Analysis of phenolic substances content of Korea plant foods. Korean Journal of Food Science and Technology 26: 310-316.
  17. Marklund, S. and Marklund, G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47: 468-74.
  18. Moon, J.S., Kim, S.J., Park, Y.M., Hwang, I.S., Kim, E.Y., Park, J.W., Park, I.B., Kim, S.W., Kang, W.G., Park, Y.K. and Jung, S.T. 2004. Anticirobial effect of methanol extracts from some medicinal herbs and content of phenolic compounds. Korean Journal of Food Preservation 11: 207-213.
  19. Pavlii, O.L. and Makarova, G.V. 1968. Studies of flavonoide of Sorbus pendula L. 3.chemical studies of substances GP-1, GP-3. Farmatsevticheskii Zhurnal 23(6): 67-72.
  20. Pryor, W.A. 1986. Oxy-radicals and related species : their formation, lifetimes, and reactions. Annual Review of Physiology 48: 657-667. https://doi.org/10.1146/annurev.ph.48.030186.003301
  21. Shin, D.H. 1997. The study course and movement of natural antioxidants. Korean of Food Science and Technology 30: 14-18.
  22. Stirpe, F. and Della Corte, E. 1969. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). The Journal of Biological Chemistry 244(14): 3855-3863.
  23. Wyngaarden, J.B. and Holmes, E.W. 1977. Molecular nature of enzyme regulation in purine biosynthsis. Ciba Foundation Symposium 48: 43-64.
  24. Yu, T.J. 1989. Sikpumbogam. Munundang, Seoul, Korea pp. 166.