• The Journal of Advanced Prosthodontics
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• v.6 no.2
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• pp.96-102
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• 2014

PURPOSE. This study was performed to characterize the effects of zirconia coated with calcium phosphate and hydroxyapatite compared to smooth zirconia after bone marrow-derived osteoblast culture. MATERIALS AND METHODS. Bone marrow-derived osteoblasts were cultured on (1) smooth zirconia, (2) zirconia coated with calcium phosphate (CaP), and (3) zirconia coated with hydroxyapatite (HA). The tetrazolium-based colorimetric assay (MTT test) was used for cell proliferation evaluation. Scanning electron microscopy (SEM) and alkaline phosphatase (ALP) activity was measured to evaluate the cellular morphology and differentiation rate. X-ray photoelectron spectroscopy (XPS) was employed for the analysis of surface chemistry. The genetic expression of the osteoblasts and dissolution behavior of the coatings were observed. Assessment of the significance level of the differences between the groups was done with analysis of variance (ANOVA). RESULTS. From the MTT assay, no significant difference between smooth and surface coated zirconia was found (P>.05). From the SEM image, cells on all three groups of discs were sporadically triangular or spread out in shape with formation of filopodia. From the ALP activity assay, the optical density of osteoblasts on smooth zirconia discs was higher than that on surface treated zirconia discs (P>.05). Most of the genes related to cell adhesion showed similar expression level between smooth and surface treated zirconia. The dissolution rate was higher with CaP than HA coating. CONCLUSION. The attachment and growth behavior of bone-marrow-derived osteoblasts cultured on smooth surface coated zirconia showed comparable results. However, the HA coating showed more time-dependent stability compared to the CaP coating.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.2
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• pp.155-162
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• 2004

Different adhesives(CF-clear, arabic gum, cethylmethyl cellulose, polyvinyl alcohol, polyvinyl pyrrolidone, hydroxy propyl cellulose, methyl cellulose) and particulate matters(bentonite, kaolin, calcium carbonate, peatmoss, talcum, vermiculite, zeolite, calcium hydroxide, plaster, calcium phosphate, vermiculite + calcium carbonate, vermiculite + kaolin, vermiculite + talcum) were evaluated by coating index method. According to the weight of single coated seeds, CF-clear and polyvinyl alcohol appeared to be the best coating adhesives for red clover and tall fescue seeds. Both vermiculite and vermiculite + talc(l : 1) for red clover, and vermiculite + talc(l : 1) for tall fescue appeared to be the best particulate matter(P < 0.01). Percent friability showed excellent results for the arabic gum, CF-clear, and polyvinyl alcohol when compared to others(p < 0.01). The coating index showed high with vermiculite and vermiculite + talc(l : 1) in coating red clover seed, and talc, vermiculite, and vermiculite + talc showed good coating index in coating tall fescue seed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.1
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• pp.67-76
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• 2000

An experiment was conducted to evaluate the effects of lime, zeolite, and rock phosphate as coating materials and coated seed size on germination, establishment, and early growth of pasture species at the experimental livestock farm, Yonam College of Agriculture in 1997. Germinating energy and germination of coated tall fescue and orchardgrass seeds were lower than those of non-coated seeds. Coated seed of tall fescue with 70% lime and 15% rock phosphate was the best in germination. The germination of small size seed (< ${\varnothing}2$ mm) was better than those of medium (> ${\varnothing}2$ mm and < ${\varnothing}2$ mm) and large size seeds (> ${\varnothing}2$ mm). Germination of coated orchardgrass seed with 55% lime and 30% rock phosphate treatment was higher than that of other coated seeds, and germination of small size seed was higher than those of medium and large size seeds (p<0.05). Germination energy and germination of coated alfalfa and birdsfoot trefoil seeds were lower than those of non-coated seeds. Germination of coated alfalfa seed with 70% lime and 15% rock phosphate, and 45% lime and 55% rock phosphate was higher compared with other coated seeds. Germination of medium size seed was higher than those of small and large size seeds (p<0.05). Among coated birdsfoot trefoil seed, 45% lime and 40% rock phosphate treatment resulted in higher germinating energy and germination than other treatments. There was no significant difference in establishment and early growth of coated tall fescue and orchardgrass seeds. However, tall fescue and orchardgrass coated with 45 % lime and 40% phosphate rock showed higher establishment and early growth. There was also no significant difference in establishment and early growth of coated alfalfa among the treatments. Among coated treatments, establishment and early growth of alfalfa coated with 75% lime and 10% rock phosphate were the highest and showed 46.4% and $72.6\;g/\textrm{m}^2$, respectively. Establishment and early growth of coated birdsfoot trefoil with 55% lime and 30% rock phosphate were the highest among coated birdsfoot trefoil seed (p<0.05) and recorded 46.4% and $44.6\;g/\textrm{m}^2$, respectively. Results of this study indicate that the germination of coated grass and legume seed may be improved by increasing the percentage of rock phosphate and decreasing that of lime. With regard to seed size, the germination of pasture species with small seed size (< ${\varnothing}2$ mm) may be better than medium and large size seeds except alfalfa. Establishment and early growth of grasses can be also improved by increasing the percentage of rock phosphate and decreasing that of lime.

• Journal of the Korean institute of surface engineering
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• v.49 no.1
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• pp.1-13
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• 2016

This review deals with one of the surface modification techniques, chemical conversion coating and particularly cerium-based conversion coatings (CeCC) as a promising substitute for chromium and phosphate conversion coating on magnesium and its alloys. The CeCCs are commonly considered environmentally friendly. The effects of surface preparation, coating thickness, bath composition, and e-paint on the corrosion behavior of CeCCs have been studied on the AZ31 magnesium alloy. This review also correlates the coating microstructural, morphological, and chemical characteristics with the processing parameters and corrosion protection. Results showed that the as-deposited coating system consists of a three layer structure (1) a nanocrystalline MgO transition layer in contact with the Mg substrate, (2) a nanocrystalline CeCC layer, and (3) an outer amorphous CeCC layer. The nanocrystalline CeCC layer thickness is a function of immersion time and cerium salt used. The overall corrosion protection was crucially dependent on the presence of coating defects. The corrosion resistance of AZ31 magnesium alloy was better for thinner CeCCs, which can be explained by the presence of fewer and smaller cracks. On the other hand, maximum corrosion protection was achieved when AZ31 magnesium samples with thin CeCCs are e-painted. The e-paint layer further restricts and hinders the movement of chloride and other aggressive ions present in the environment from reaching the magnesium surface.

• Journal of the Korean Vacuum Society
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• v.12 no.S1
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• pp.10-14
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• 2003

Coatings of hydroxyapatite (HA) and tricalcium phosphate/HA (TCP/HA) on titanium were fabricated by ion beam assisted deposition (IBAD). Significant effect of the Ca-P coatings on human Gingival Fibroblasts (HGFs) attachment and formation of type I collagen were found by using immunofluorescence microscope. TCP/HA and HA coatings exerted more HGFs attachment and collagen I formation. Comparing with HA coating, TCP/HA coating exhibited better responses during the late period of the tests. This investigation indicated that this surface modification method may enhance the biological seal at the cervical level of the titanium dental implants.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09a
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• pp.160-161
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• 2006

Seasonal changes have been recognized in particle characteristics and forming characteristics of iron powder with insulated coating for a compacted magnetic core because of its high hygroscopicity, due to its phosphate coating and resin binder additives. For this reason, particle characteristics and molding characteristics of the powder with diverse water absorbtivity have been studied. The result shows that the higher the volume of absorbed water, the worse the fluidity becomes, resulting in the reduction in both springback during the molding process and expansion reduction after the heat treatment. The requirement on dimension accuracy for the finished product can be satisfied with an additional drying process on the material powder, which contributes to maintain its water volume constant.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.29 no.3
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• pp.151-156
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• 2003

This study was performed to develop a useful nerve conduit which provides favorable environment for Schwann cell viability and proliferation. Milipore membrane of $0.45{\mu}m$ pore size was selected because it permits nutritional inflow from the outside of the conduit and prevents from invading the fibrotic tissue into the conduit. The membrane was rolled and sealed to form a conduit of 2mm diameter and 20mm length. To improve the axonal regeneration and to render better environment for endogenous and exogenous Schwann cell behaviour, the microgeometry and surface of conduit was modified by coating with thin film of calcium phosphate. Cellular viability within the conduit and attachment to its wall were assessed with MTT assay and SEM study. Milipore filter conduit showed significantly higher rate of Schwann cell attachment and viability than the culture dish. However, the reverse was true in case of fibroblast. Coating with thin film of low crystalline calcium phosphate made more favorable environment for both cells with minimal change of pore size. These findings means the porous calcium phosphate coated milipore nerve conduit can provide much favorable environment for endogenous Schwann cell proliferation and exogenous ones, which are filled within the conduit for the more advanced strategy of peripheral nerve regeneration, with potential of reducing fibrotic tissue production.

• Journal of Periodontal and Implant Science
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• v.33 no.4
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• pp.651-671
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• 2003

The influence of calcium phosphate (Ca-P) coating on the bone response of titanium implants was investigated two types of titanium implants, i.e. as -machined ,as -machined with Ca-P coating, were prepared. The Ca-P coating produced by OCT Inc technique. These implants were inserted into the left and right femur of beagle dog, After implantation periods of 3 days, 1weeks, weeks, 4weeks, 8weeks, 12weeks. 24weeks, the bone-implant interface was evaluated histologically, histomorphometrically , and removal torque. Histological evaluation revealed no new bone formation around different implant materials after 2weeks of implantation. After 4 weeks, Ca-P coated implants showed a higher amount of bone contact than either of the non coated implants. After 12weeks, bone healing was almost completed. And implant were removed by reverse torque rotation with torque-measuring device. Mean torque values for 4weeks control were 2.375Kgf.cm and experimental were 2.725Kgf.cm. And mean torque values for 8weeks control were 1.25Kgf.cm and experimental were 1.0Kgf.cm On the basis of these findings, we concluded that deposition of a Ca-P coating on an implant has a beneficial effect on the bone response to this implant during the healing phase. Besides implant surface conditions the bone response is also determined by local implant site condition.

• Korean Journal of Materials Research
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• v.20 no.8
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• pp.418-422
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• 2010

As a starting material, BCP (biphasic calcium phosphate) nano powder was synthesized by a hydrothermal microwave-assisted process. A highly porous BCP scaffold was fabricated by the sponge replica method using 60 ppi (pore per inch) of polyurethane sponge. The BCP scaffold had interconnected pores ranging from $100\;{\mu}m$ to $1000\;{\mu}m$, which were similar to natural cancellous bone. To realize the antibacterial property, a microwave-assisted nano Ag spot coating process was used. The morphology and distribution of nano Ag particles were different depending on the coating conditions, such as concentration of the $AgNO_3$ solution, microwave irradiation times, etc. With an increased microwave irradiation time, the amount of coated nano Ag particles increased. The surface of the BCP scaffold was totally covered with nano Ag particles homogeneously at 20 seconds of microwave irradiation time when 0.6 g of $AgNO_3$ was used. With an increased amount of $AgNO_3$ and irradiation time, the size of the coated particles increased. Antibacterial activities of the solution extracted from the Ag-coated BCP scaffold were examined against gram-negative (Escherichia coli) and gram-positive bacteria (Staphylococcus aureus). When 0.6 g of $AgNO_3$ was used for coating the Ag-coated scaffold, it showed higher antibacterial activities than that of the Ag-coated scaffold using 0.8 g of $AgNO_3$.

• Corrosion Science and Technology
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• v.6 no.5
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• pp.239-244
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• 2007

Corrosion protection of automotive steels has traditionally been assured by using a zinc phosphate metal pretreatment followed by the deposition of a cathodic electrocoat system. This system has been developed and optimized over the years into a highly robust and dependable system with a high performance. However, in terms of efficiency and use of resources and energy, the need is now felt to develop a simpler system with fewer steps, shorter lines, less energy requirements (curing and e-coat deposition) and less stringent waste disposal requirement (phosphate sludge). We report here on the development of a one-step system that can possibly replace both the zinc phosphate and the e-coating processes. Such a system is based on the so-called superprimer concept that we have recently developed for the replacement of chromate pretreatment and chromate-containing primers in the aerospace industry. With some modifications, such systems can also be adapted for use in the automotive industry.