• 대한물리치료과학회지
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• 제18권3호
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• pp.87-92
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• 2011

Purpose : The purpose of this study is to have examined the influence on the blood circulation by comparing the differences between stimulating the sympathetic ganglion and the muscle group among the stimulation variables in interferential current stimulation and to have found out the most effective stimulating mode for the improvement the peripheral blood circulation in the interferential current stimulation. Method : The subjects of the study is the men and women in the twenties, who are in great condition and have no pathological report for the blood circulation influence. The intensity of the inferential current stimulation is the medium degree, 100 bps constant current, which is the comfort and overt degree to confirm the muscle contraction. The areas stimulated by the interferential current stimulation are the stellate ganglion area in the seventh cervical vertebrae and the forearm muscle area. The stimulating time is twenty minutes long. After stimulating the two areas, the change of the blood circulation has been measured. Results : Both stimulating the sympathetic ganglion and stimulating the muscle, before and after interferential current stimulation, we have seen that the amount of blood circulation was increased significantly and statistically in both two groups(p<.01) and that the amount of blood circulation was increased significantly and statistically in the case of stimulating the sympathetic ganglion(p<.01). Conclusion : The conclusion we received that inferential current stimulation had the clear influence on increasing the peripheral blood circulation. And stimulating the sympathetic ganglion area is more effective than stimulating muscle area directly.

• IMMUNE NETWORK
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• 제14권6호
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• pp.289-295
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• 2014

Flow cytometric immunophenotyping of peripheral blood lymphocyte subsets is a powerful tool for evaluating cellular immunity and monitoring immune-mediated diseases. The numbers and proportions of blood lymphocyte subsets are influenced by factors such as gender, age, ethnicity, and lifestyle. This study aimed to establish reference ranges for peripheral blood lymphocyte subsets in a healthy Korean population. Blood samples from 294 healthy adults were collected. Lymphocyte subsets were analyzed using a single-platform method with a flow cytometer; white blood cells and lymphocytes were analyzed using an automated hematology analyzer. The mean value of the white blood cell count was $5,665cells/{\mu}l$, and the mean values of the subtype counts (percentages) were as follows: lymphocytes, $1,928cells/{\mu}l$ (35.08%); $CD3^+$ cells, $1,305cells/{\mu}l$ (67.53%); $CD3^+CD4^+$ cells, $787cells/{\mu}l$ (40.55%); $CD3^+CD8^+$ cells, $479cells/{\mu}l$ (25.23%); $CD3^-CD19^+$ cells, $203cells/{\mu}l$ (10.43%); and $CD3^-CD56^+$ cells, $300cells/{\mu}l$ (15.63%). Additionally, the $CD4^+/CD8^+$ ratio was 1.81. In this study, gender and age significantly influenced blood lymphocyte subsets. Our results demonstrate that, as with other populations, a healthy Korean population has its own, region-specific, lymphocyte subset reference ranges.

• 대한방사선협회지
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• 제27권2호
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• pp.181-197
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• 2001

Thus, among peripheral blood, the number of WBC decreased drastically for a certain period after total body irradiation with high dose radiation, and the levels of Seg. neutriphil and lymphocyte decreased significantly after irradiation in the lymphocyte

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.429.1-429.1
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• 2002

In the patients with hematologic and solid tumors. clinical application of peripheral blood stem cells (PBSCs) increases to reconstitute hematopoiesis after high-dose chemotherapy. To mobilize PBSCs. the hematopietic growth factors have been widely used as single treatment or after chemotherapy. The important issue in the collection of PBSCs is to predict their potential capability to reconstitute hematopoiesis. which depends on optimal time of leukapheresis and the quantity of collected PBSCs. (omitted)

• Tuberculosis and Respiratory Diseases
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• 제42권5호
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• pp.654-659
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• 1995

연구배경: 결핵에서의 혈액학적인 변화는 그 양상이 다양하며, 이러한 변화는 단순폐결핵과 속립성결핵에서 차이가 있다고 알려져 있다. 지금까지 국내에서 단순폐결핵에 대한 말초 혈액의 혈액학적 변화에 대한 연구는 있었으나, 속립성결핵에서의 혈액학적 변화에 대한 보고는 거의 없는 실정이다. 이에 속립성결핵에서 말초혈액 및 골수에서의 혈액학적 변화를 알아보고자 본 연구를 시행하였다. 방법 1993년 8월부터 1994년 7월까지 전북대학교 병원에 입원한 환자들 중 단순흉부방사선사진 및 고해상도 전산화 단층사진상 속립성 결핵 소견을 보이며 객담도말과 배양검사, PCR검사에서 결핵균이 증명되었거나 골수검사에서 결핵성육아종을 보인 10예를 대상으로 하였다. 혈액학적 소견의 평가는 Dacie 등에 의해 기술되어진 정의에 따랐으며 혈액체취는 내원당시에 시행하였고 골수검채는 입원후 7일이내에 시행하였다. 결과: 말초혈액소견상 범혈구감소증 1예, 빈혈은 전예에서 있었으며 백혈구증가증은 10%, 백혈구감소증은 20%, 혈소판감소증은 30%, 임파구감소증은 90%, 단구증가증은 40%, 중성구증가증은 20% 그리고 중성구감소증은 환자의 10%에서 나타났다. 골수검사에서 임파구감소증은 30%, 임파구증가증은 20%, 형질세포감소증은 40%, 단구증가증은 전예(100%)에서 나타났으며, 저세포충실도는 30%, 적혈구조혈감소는 30% 그리고 과립구조혈증가와 감소가 각각 환자의 20%에서 나타났다. 골수육아종소견은 환자의 25%에서 나타났다. 결론: 속립성결핵의 혈액학적 변화는 혈구감소증의 경향을 보였으나 단구는 말초 및 골수 모두에서 증가하는 소견을 보였다. 이러한 소견은 속립성결핵의 감별진단에 있어서 도움을 줄 수 있올 것으로 기대된다.

• BMB Reports
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• 제53권1호
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• pp.10-19
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• 2020

Alzheimer's disease (AD) is a chronic neurodegenerative disease characterized by the accumulation of amyloid plaques and neurofibrillary tangles in the brain. The AD pathophysiology entails chronic inflammation involving innate immune cells including microglia, astrocytes, and other peripheral blood cells. Inflammatory mediators such as cytokines and complements are also linked to AD pathogenesis. Despite increasing evidence supporting the association between abnormal inflammation and AD, no well-established inflammatory biomarkers are currently available for AD. Since many reports have shown that abnormal inflammation precedes the outbreak of the disease, non-invasive and readily available peripheral inflammatory biomarkers should be considered as possible biomarkers for early diagnosis of AD. In this minireview, we introduce the peripheral biomarker candidates related to abnormal inflammation in AD and discuss their possible molecular mechanisms. Furthermore, we also summarize the current state of inflammatory biomarker research in clinical practice and molecular diagnostics. We believe this review will provide new insights into biomarker candidates for the early diagnosis of AD with systemic relevance to inflammation during AD pathogenesis.

• Tuberculosis and Respiratory Diseases
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• 제74권6호
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• pp.264-268
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• 2013

Background: Idiopathic pulmonary fibrosis (IPF) is a lethal pulmonary fibrotic disease. In general, the exaggerated activation of the coagulation cascade has been observed during initiation or maintenance of the fibrotic disease. In our recent study, immunohistochemical expression of protease-activated receptor-2 (PAR-2), which plays a key role in coagulation cascade, was observed in surgical specimen of IPF patients, and associated with poor clinical outcome. The aim of this study was to evaluate the overexpression of PAR-2 in inflammatory cells from peripheral blood and bronchoalveolar lavage fluid in IPF patients. Methods: From May 2011 to March 2012, IPF patients and controls were enrolled in Seoul National University Hospital. Peripheral blood and bronchoalveolar lavage fluid were collected for analysis of PAR-2 expression. Flow cytometry and reverse transcription polymerase chain reaction were used for PAR-2 receptor and mRNA assessment. Results: Twelve IPF patients and 14 controls were included in this study. Among them, flow cytometry analysis was conducted from 26 peripheral blood (patient group, 11; control group, 13) and 7 bronchoalveolar lavage fluid (patient group, 5; control group, 2). The expression of PAR-2 receptor was not different between patient and control groups (p=0.074). Among all 24 population, PAR-2 mRNA assessment was performed in 19 persons (patient group, 10; control group, 9). The mRNA expression of PAR-2 was not significant different (p=0.633). Conclusion: In IPF patients, PAR-2 receptor and mRNA expression were not different from control group.

• The Korean Journal of Pain
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• 제31권1호
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• pp.43-49
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• 2018

Background: Chronic pain reportedly exerts complex effects on immune function. Natural killer (NK) cells are lymphocytes that play a critical role in cellular and innate immunity. This study examined changes in the subset populations and cytotoxic activity of peripheral blood NK cells in patients with chronic pain. Methods: Thirty patients with chronic moderate-to-severe pain (group P) and age-matched pain-free subjects (group NoP) were enrolled. Peripheral whole blood was analyzed for the percentage and expression of NK cell surface markers (CD56 and CD16) by flow cytometry. Cytotoxic activity was assayed by evaluating CD69 expression on $CD3^-/CD56^+NK$ cells. Results: The percentage of NK cells among total lymphocytes was not significantly different between groups P and NoP ($16.3{\pm}9.3$ vs. $20.2{\pm}10.5%$). Likewise, the percentages of two major NK cell subsets, $CD56^{bright}$ and $CD56^{dim}$, were also not significantly different between the two groups. However, the percentage of $CD56^{bright}/CD16^+$ subset, was slightly but significantly increased in group P ($1.0{\pm}0.9%$; P< 0.01) compared with group NoP ($0.5{\pm}0.6%$). The cytotoxicity of NK cells was not different between the two groups, showing similar CD69 expression (P vs. $NoP=29.2{\pm}15.2$ vs. $32.0{\pm}15.0%$). These findings were not influenced by pain intensity, opioid use, or disease causing pain in group P. Conclusions: NK cell cytotoxic activity and major subset populations, with the exception of an increased percentage of the $CD56^{bright}/CD16^+$ subset, are not significantly altered in patients with chronic severe pain.

• 한국동물위생학회지
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• 제30권1호
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• pp.51-66
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• 2007

The purpose of this study was to evaluate the effect of a subsequent infection of porcine reproductive and respiratory syndrome(PRRS) virus to pigs with A pleuropneumonia. Twenty three 7-week-old commercial pigs were infected intratracheally with PRRS virus and/or A pleuropneumoniae serotype 5. Serum antibody titers were examined by an enzyme-linked immunosorbent assay(ELISA) and proportion of porcine leukocyte subpopulations in peripheral blood was examined by flow cytometry. In this experiment, antibodies against PRRS virus and A pleuropneumoniae were detected at 2 weeks and 1 week postinfection and the number of antibody positive pigs were gradually increased. And in proportion to leukocyte subpopulations in peripheral blood of pigs infected with A pleuropneumoniae compared with pigs administrated with saline, the proportion of PoCD4 and N cells were increased(P<0.1). Furthermore, in proportion to leukocyte subpopulations in peripheral blood of pigs infected with PRRS virus followed by A pleuropneumoniae compared with pigs administrated with saline, the proportion of MHC class II, PoCD4 and B cells were significantly increased(P<0.1). The results indicated that dual infection with PRRS virus and A pleuropneumoniae induced the stronger immune responses associated with macrophages and Th cells in pigs than single infection with PRRS virus or A pleuropneumoniae.

• 한국임상수의학회지
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• 제40권3호
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• pp.175-181
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• 2023

Fucoidan extracted from brown seaweed has a variety of biological activities. Neutrophil extracellular traps (NETs) formation is an immune response for the invasion of pathogens. Neutrophils release granule protein and chromatin that form extracellular fibers that bind microbes. These NETs degrade virulence factors and kill bacteria. The aim of this study was to investigate the effect of fucoidan on NET formation of porcine peripheral blood polymorphonuclear cells (PMNs). The NET formation was determined by fluorescence emission of propidium iodide (PI) in PMNs by a fluorescence microplate reader. The production of tumor necrosis factor (TNF)-α from peripheral blood mononuclear cells (PBMCs) was measured by ELISA method. Fucoidan itself did not show any direct effect on NET formation. However, NET formation of PMNs was increased by the culture supernatant from PBMCs treated with fucoidan. The NET formation of PMNs were also enhanced by treatment with recombinant porcine (rp) TNF-α. The ability of culture supernatant from PBMCs treated with fucoidan to increase the NET formation of PMNs was inhibited by addition of goat anti-rp TNF-α polyclonal antibody (pAb) (IgG) prior to the culture. The increase of NET formation by rp TNF-α was also inhibited by goat anti-rp TNF-α pAb (IgG). The level of TNF-α in culture supernatant from PBMCs was increased by treatment with fucoidan. These results suggest that fucoidan increases porcine NET formation, which is mediated by TNF-α produced from PBMCs.