• Nutrition Research and Practice
• /
• v.10 no.3
• /
• pp.274-281
• /
• 2016

BACKGROUND/OBJECTIVES: The accumulation of amyloid-${\beta}$ ($A{\beta}$) in the brain is a hallmark of Alzheimer's disease (AD) and plays a key role in cognitive dysfunction. Perilla frutescens var. japonica extract (PFE) and its major compound, rosmarinic acid (RA), have shown antioxidant and anti-inflammatory activities. We investigated whether administration of PFE and RA contributes to cognitive improvement in an $A{\beta}_{25-35}$-injected mouse model. MATERIALS/METHODS: Male ICR mice were intracerebroventricularly injected with aggregated $A{\beta}_{25-35}$ to induce AD. $A{\beta}_{25-35}$-injected mice were fed PFE (50 mg/kg/day) or RA (0.25 mg/kg/day) for 14 days and examined for learning and memory ability through the T-maze, object recognition, and Morris water maze test. RESULTS: Our present study demonstrated that PFE and RA administration significantly enhanced cognition function and object discrimination, which were impaired by $A{\beta}_{25-35}$, in the T-maze and object recognition tests, respectively. In addition, oral administration of PFE and RA decreased the time to reach the platform and increased the number of crossings over the removed platform when compared with the $A{\beta}_{25-35}$-induced control group in the Morris water maze test. Furthermore, PFE and RA significantly decreased the levels of nitric oxide (NO) and malondialdehyde (MDA) in the brain, kidney, and liver. In particular, PFE markedly attenuated oxidative stress by inhibiting production of NO and MDA in the $A{\beta}_{25-35}$-injected mouse brain. CONCLUSIONS: These results suggest that PFE and its active compound RA have beneficial effects on cognitive improvement and may help prevent AD induced by $A{\beta}$.

• The Plant Pathology Journal
• /
• v.17 no.4
• /
• pp.236-241
• /
• 2001

Serve outbreaks of anthracnose were observed on perilla plants grown in greenhouses and open fields in several locations in Korea during the disease survey from 1997 to 2000. A total of 53 isolates of Colletotrichum spp. and Glomerella sp. was obtained from diseased perilla plants and identified based on their morphological and cultural characteristics. Forty isolates were identified as Colletotrichum gloeosporioides, three isolates as C. coccodes, five isolates as C. dematium, and the other five isolates as Glomerella cingulata, the teleomorph of C. gloeosporioides. All isolates of C. gloeosporioides tested by artificial inoculation were strongly virulent on perilla plants, but isolates of the other species were weakly or not virulent. Anthracnose symptoms induced on the perilla plants by artificial inoculation with the isolates of C. gloeosporioides were similar to those observed in the fields. This study revealed that C. gloeosporioides is the main causal fungus of perilla anthracnose.

• Natural Product Sciences
• /
• v.22 no.4
• /
• pp.259-262
• /
• 2016

The content analysis of fatty acids in Perilla cultivars and commercial oils is conducted through gas chromatography with a flame ionization detector. Results show that Perilla cultivars, such as Deulsaem and Daesil, contain high amounts of ${\alpha}-linolenic$ acid (262.22 and 261.97 mg/g, respectively). Among commercial oils, Perilla oil contains a higher amount of ${\alpha}-linolenic$ acid (515.20 mg/g). Accordingly, ${\alpha}-linolenic$ acid is a major fatty acid of Perilla cultivars and oil. Therefore, Perilla cultivars could be used as a food supplement for nutritional and pharmaceutical purposes.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2007.11a
• /
• pp.362.1-362.1
• /
• 2007

• Korean Journal of Breeding Science
• /
• v.43 no.4
• /
• pp.288-296
• /
• 2011

To clarify the seed germination characteristics among cultivated Perilla crop and their weedy types in Korea, we studied the germination percent and germination energy of 162 accessions (102 cultivated var. frutescens, 41 weedy var. frutescens, and 19 weedy var. crispa) in both conditions of $4^{\circ}C$ low temperature treatment and non-cold treatment. In our study, most accessions of cultivated var. frutescens showed more than 50% in both germination percent and germination energy in both cold and non-cold treatment conditions. Whereas, most accessions of weedy var. frutescens and weedy var. crispa showed lower than 50% in both germination percent and germination energy in both cold and non-cold treatment. In addition, most accessions of Perilla crop and their weedy types showed much higher germination percent and germination energy in $4^{\circ}C$ low temperature treatment condition compared to the seeds under non-cold treatment condition. The information provided in this research may help for our understanding the variation of seed germination characteristics among accessions of cultivated Perilla crop and their weedy types in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.36 no.2
• /
• pp.143-146
• /
• 1991

The present experiment was carried out to investigate the effects of direct sowing time culture of Perilla frutescens var. acuta KUPO on growth yield and it's components. The number of days from seeding to emergence was recognized linear negative correlation between seeding dates. Stem length was the longest in Apr. 1 seeding, and was short in the early, and the late seeding. Stem diameter was the thickest in Apr. 1 seeding, and was thin in the early, and the late seeding. Dry weight of leaf and stem was increased on Apr. 1 seeding. Grain yield was the highest in Apr. 1 seeding date. Therefore, optimum time for direct sowing was on Apr. 1.

• KSBB Journal
• /
• v.19 no.3
• /
• pp.187-191
• /
• 2004

Among 14 medicinal plants selected for the study ethanol (70%) extract of Coptis japonica Makino showed the highest anti-microbial activity against Helicobacter pylori followed by Perilla frutescens var. acuta KUDO, Caesalpinia sappan L. and Schizonepeta tenuifolia Briq. However, anti-urease activity of methanol (80％) extracts was best for Forsythiae Fructus followed by Caesaipinia sappan L. and Schizonepeta tenuifolia Briq. In the second fractionation using water, ethyl acetate and butanol more than 90％ of the anti-urease activity was detected in the ethyl acetate fraction.

• Journal of Environmental Science International
• /
• v.19 no.10
• /
• pp.1301-1305
• /
• 2010

This study was carried out to investigate the effect of KEM and SAF appication on contents change of fatty acids and organic acid of perilla(Perilla Frutescens Britton). Contenst of squalene in perilla leaves on control and KEM/SAF treated were 3.39 mg and 4.22 mg, respectively. Therefore the squalene quantity of KEM/SAF treated leaves was 24.2% more than that of control. A total 6 fatty acids in perilla leaves were analyzed in this study. Percentage of the saturated and unsaturated fatty acid in perilla leaves were 20 and 80%, respectively. Contents of phytosterols in perilla leaf such as campesterol and sitosterol were 2.0 and 20.0 mg, respectively. Therefore sitosterol content was 10 fold more than that of campesterol. The KEM/SAF application on perilla leaf was effective on the change of squalene or phytosterol contents. However effect of that was negligible on the change of fatty acid content.

• Applied Biological Chemistry
• /
• v.50 no.4
• /
• pp.327-333
• /
• 2007

This study was conducted to examine antioxidant activities of Perilla frutescens var. acuta leaf. For the this purpose, DPPH radical scavenging activity, lipid oxidation inhibition, SOD-like activity, and xanthine oxidase inhibitor activity of water extract, ethanol extracts (30, 50, 70, and 95%) and the fractions obtained from these extracts were determined. The electron donating abilities of the chloroform fraction obtained from the 70% and 95% ethanol extracts were 50%, and that of the ethyl acetate fraction for all of the extracts was above 75%. In particular, the electron donating ability of the ethyl acetate fraction of the 70% ethanol extract showed the greatest activity with 200.5 ppm of $RC_{50}$ value. The 70% ethanol extract was most effective to inhibit the automatic oxidation of linoleic acid at $40^{\circ}C$ storage. The highest inhibition effects appeared in the chloroform and ethyl acetate fractions of the water extract, and the 30, 50, and 70% ethanol extracts, and the highest lipid oxidation inhibiting effect of the 95% ethanol extract occurred in the hexane and acetate fractions. The SOD-like activity of the water extract was 30.3%, and the activities of the various concentration of ethanol extracts were 28-32% and the activity of the 70% ethanol extract was the highest. The SOD-like activity of the ethyl acetate fraction of the 70% ethanol extract was highest with 1,549.0 ppm of $RC_{50}$ value. Xanthine oxidase inhibition activity was greatest in the water extract and the activities of the ethanol extracts were 36-41.2%. The xanthine oxidase inhibition activity of the ethyl acetate fraction of the water extract was highest. In summary, we found that electron donating ability, lipid oxidation inhibition, and SOD-like activity of Perilla frutescens var. acuta leaf were greatest in the ethyl acetate fraction of the 70% ethanol extract, and xanthine oxidase inhibition activity was highest in the ethyl acetate fraction of the water extract.

• Korean Journal of Plant Resources
• /
• v.12 no.3
• /
• pp.204-208
• /
• 1999

We carried out this study to confirm the possibility of C. wilfordii cultivation using crop as a substitute for stake or net, and tested sit support crops, i.e., soybean, peanut, Perilla frutescens var. acuta Kudo, Job's tears, sorghum, and corn compared with net support. Effects of support crops on growth and root yield of C. wilfordii were as follows. Attracting efficiency of vine showed 94∼100% among crops. The number of branches per plant was 1 or 2 more in p. frutescens var. acuta Kudo, Job's tears, and sorghum support than in net support. Fresh root yield per 10a was 919kg in net support, while it was 717kg in P. frutescens var. acute Kudo, 656kg in Job' tears, and 652kg in sorghum support. However, the problem of decreased yield in these support crops could be overcome by cost saving of staking installation and by harvesting support crops. Therefore, C. wilfordii cultivation using P. frutescens var. acute Kudo, Job's tears, and sorghum as support crops showed most effective in labor and cost saving.